1. Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution
- Author
-
Chih-Yung Lin, Jian Jheng Wu, Chih-Yang Lin, Chao Chun Chuang, Yung Chang Chen, Chao-Yuan Yeh, Chang Huain Hsieh, Chang Wei Yeh, Hui-Hao Lin, Guan-Yu Chen, Jenn-Kang Hwang, Cheng Chi Wu, Hao Wei Hsu, Ann-Shyn Chiang, Hsin Jung Chiang, Jing Yi Chen, Hsiu Ming Chang, Chia Chou Wu, Guo Tzau Wang, Ping Chang Lee, Chun Fang Lu, Yu-Tai Ching, Chi-Tin Shih, Ru Fen Ni, and Yun Ann Huang
- Subjects
Male ,Neurons ,Agricultural and Biological Sciences(all) ,Biochemistry, Genetics and Molecular Biology(all) ,Information processing ,Brain ,Wiring diagram ,Biology ,Local variation ,biology.organism_classification ,Bioinformatics ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Visualization ,Biological neural network ,Animals ,Computer Simulation ,Drosophila ,Female ,Drosophila (subgenus) ,General Agricultural and Biological Sciences ,Projection (set theory) ,Neuroscience ,Resolution (algebra) - Abstract
Summary Background Animal behavior is governed by the activity of interconnected brain circuits. Comprehensive brain wiring maps are thus needed in order to formulate hypotheses about information flow and also to guide genetic manipulations aimed at understanding how genes and circuits orchestrate complex behaviors. Results To assemble this map, we deconstructed the adult Drosophila brain into approximately 16,000 single neurons and reconstructed them into a common standardized framework to produce a virtual fly brain. We have constructed a mesoscopic map and found that it consists of 41 local processing units (LPUs), six hubs, and 58 tracts covering the whole Drosophila brain. Despite individual local variation, the architecture of the Drosophila brain shows invariance for both the aggregation of local neurons (LNs) within specific LPUs and for the connectivity of projection neurons (PNs) between the same set of LPUs. An open-access image database, named FlyCircuit, has been constructed for online data archiving, mining, analysis, and three-dimensional visualization of all single neurons, brain-wide LPUs, their wiring diagrams, and neural tracts. Conclusion We found that the Drosophila brain is assembled from families of multiple LPUs and their interconnections. This provides an essential first step in the analysis of information processing within and between neurons in a complete brain.
- Published
- 2011
- Full Text
- View/download PDF