1. INFLUENCE OF HYPOXIA AND HYPOXIA-REOXYGENATION ON ENDOTHELIAL P-SELECTIN EXPRESSION
- Author
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Christèle Closse, Martine Renard, Patrice Dumain, Francis Belloc, Martine Seigneur, Annie Pruvost, and Michel R. Boisseau
- Subjects
Umbilical Veins ,Pathology ,medicine.medical_specialty ,Endothelium ,P-selectin ,Neutrophils ,Surface Properties ,Ischemia ,Biology ,Hypoxic exposure ,Umbilical vein ,Umbilical Cord ,Flow cytometry ,Andrology ,Physiology (medical) ,Gene expression ,medicine ,Humans ,Hypoxia ,Cells, Cultured ,medicine.diagnostic_test ,Chemistry ,Hematology ,Hypoxia (medical) ,Flow Cytometry ,medicine.disease ,Cell Hypoxia ,Oxygen ,P-Selectin ,Spectrometry, Fluorescence ,medicine.anatomical_structure ,Gene Expression Regulation ,Cell culture ,Reperfusion ,Hypoxia reoxygenation ,Endothelium, Vascular ,medicine.symptom ,Neutrophil recruitment - Abstract
P-selectin is an endothelial adhesion molecule involved in the initial step of the neutrophil recruitment. We investigated the effect of hypoxia (95% N2, 5% CO2) and of hypoxia-reoxygenation (95% air, 5% CO2) on the expression of P-selectin by human umbilical vein endothelial cells (HUVEC). P-selectin expression was detected by immunolabelling and quantified by flow cytometric analysis. Our data indicate that hypoxia induces an increase in P-selectin expression with a maximum reached after 90 minutes. A hypoxic exposure of 90 minutes results in a highly significant increase compared to normoxia (p < 0.001, n=13). Furthermore, when a reoxygenation period follows 90 minutes of hypoxia, the initially elevated levels of P-selectin are dramatically enhanced with a maximum obtained after 60 minutes of reoxygenation.
- Published
- 1997
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