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Phage-microbe interactions are appealing systems to study coevolution, and have also been increasingly emphasized due to their roles in human health, disease, and the development of novel therapeutics. Phage-microbe interactions leave diverse signals in bacterial and phage genomic sequences, defined as phage-host interaction signals (PHISs), which include clustered regularly interspaced short palindromic repeats (CRISPR) targeting, prophage, and protein-protein interaction signals. In the present study, we developed a novel tool phage-host interaction signal detector (PHISDetector) to predict phage-host interactions by detecting and integrating diverse in silico PHISs, and scoring the probability of phage-host interactions using machine learning models based on PHIS features. We evaluated the performance of PHISDetector on multiple benchmark datasets and application cases. When tested on a dataset of 758 annotated phage-host pairs, PHISDetector yields the prediction accuracies of 0.51 and 0.73 at the species and genus levels, respectively, outperforming other phage-host prediction tools. When applied to on 125,842 metagenomic viral contigs (mVCs) derived from 3042 geographically diverse samples, a detection rate of 54.54% could be achieved. Furthermore, PHISDetector could predict infecting phages for 85.6% of 368 multidrug-resistant (MDR) bacteria and 30% of 454 human gut bacteria obtained from the National Institutes of Health (NIH) Human Microbiome Project (HMP). The PHISDetector can be run either as a web server (http://www.microbiome-bigdata.com/PHISDetector/) for general users to study individual inputs or as a stand-alone version (https://github.com/HIT-ImmunologyLab/PHISDetector) to process massive phage contigs from virome studies.

Summary Whole-genome sequencing (WGS), a powerful tool for detecting novel coding and non-coding disease-causing variants, has largely been applied to clinical diagnosis of inherited disorders. Here we leveraged WGS data in up to 62,653 ethnically diverse participants from the NHLBI Trans-Omics for Precision Medicine (TOPMed) program and assessed statistical association of variants with seven red blood cell (RBC) quantitative traits. We discovered 14 single variant-RBC trait associations at 12 genomic loci, which have not been reported previously. Several of the RBC trait-variant associations (RPN1, ELL2, MIDN, HBB, HBA1, PIEZO1, and G6PD) were replicated in independent GWAS datasets imputed to the TOPMed reference panel. Most of these discovered variants are rare/low frequency, and several are observed disproportionately among non-European Ancestry (African, Hispanic/Latino, or East Asian) populations. We identified a 3 bp indel p.Lys2169del (g.88717175_88717177TCT[4]) (common only in the Ashkenazi Jewish population) of PIEZO1, a gene responsible for the Mendelian red cell disorder hereditary xerocytosis (MIM: 194380 ), associated with higher mean corpuscular hemoglobin concentration (MCHC). In stepwise conditional analysis and in gene-based rare variant aggregated association analysis, we identified several of the variants in HBB, HBA1, TMPRSS6, and G6PD that represent the carrier state for known coding, promoter, or splice site loss-of-function variants that cause inherited RBC disorders. Finally, we applied base and nuclease editing to demonstrate that the sentinel variant rs112097551 (nearest gene RPN1) acts through a cis-regulatory element that exerts long-range control of the gene RUVBL1 which is essential for hematopoiesis. Together, these results demonstrate the utility of WGS in ethnically diverse population-based samples and gene editing for expanding knowledge of the genetic architecture of quantitative hematologic traits and suggest a continuum between complex trait and Mendelian red cell disorders.

Photocatalytic hydrogen evolution from water is a valid method to face the challenge of energy shortage. TiO2, as the most well-known and investigated semiconductor photocatalyst, has been extensively applied in this field. However, the hydrogen evolution performance of TiO2 suffer from low efficiency due to the high combination of photo-induced carries and the narrow light utilization range resulting from wide band gap. In this work, we use the commercial TiO2 (P25) and fullerene (C60) as precursors to construct an efficient photocatalyst via a mild mixed method. The introduction of C60 not only effectively broaden light absorption, but also suppressed the recombination of photo-generated carriers on TiO2. The photocatalytic water splitting property of the optimum sample achieve 597.2 μmol g−1 h−1, which is 31 times higher than pure P25.

Using a sample of A-share listed companies on the Shanghai and Shenzhen Stock Exchanges, this study examines the impact of voluntary corporate social responsibility (CSR) disclosure on independent audit demand in different institutional environments. The sample covers the 2008–2016 period and excludes firms that are required to disclose CSR information. The findings show that compared with companies that choose to not disclose such information, companies that voluntarily disclose CSR information prefer to employ “small” accounting firms for auditing services, although the auditing fees are much higher. However, as the institutional environment improves, this preference is weakened. Further analyses show that this preference is stronger in enterprises where CSR disclosure is driven by strong political motivations and managerial self-interest motivations. Legitimate motivations and economic motivations do not significantly impact the preference for “small” audit firms. Furthermore, this preference is more obvious in enterprises with poor operational performance and low information disclosure quality, and it increases as CSR disclosure level decreases. The study enriches our understanding of the economic consequences of voluntary CSR disclosure and the factors that influence independent audit demand. The results also have implications for the construction and regulation of China’s system for supervising CSR information disclosure in the transitional phase. Keywords: Voluntary disclosure of corporate social responsibility information, Auditor selection, Auditing fees, Institutional environment

We assessed the efficacy of Ca-bentonite (CB) alone and combined with Ca-hydroxide (CH), tobacco biochar (TB), and zeolite (ZL) aiming to immobilize Cu and Pb and decrease their bioavailability and uptake by pak choi followed by maize in a mining contaminated soil. The CB alone was able to decrease the availability and uptake of Cu and Pb by pak choi and maize. The mono- and multi-combination of CH, TB, and ZL with CB showed contradictory impact on the availability and uptake of Cu and Pb as compared to the mono-application of CB. The combination of CB with ZL and CH + ZL reduced the uptake of Pb by pak choi and maize, while the combination of CB with TB and ZL reduced the uptake of Cu by pak choi and maize as compared to the mono-application of CB. The co-application of CB with CH increased the phytoextraction of Cu by maize and Pb by pak choi shoots as compared to the mono-application of CB. We conclude that modified clays such as CB alone or combined with ZL, TB, and/or CH might be suitable candidates for phytomanagement of Cu and Pb contaminated soils.

Thermal conversion of phytoremediation plant waste into biochar was performed as a safe approach for organic waste management. In this work, a promising phytoremediation plant―Silphium perfoliatum L. was pyrolyzed for biochar production at 350, 550 and 750 °C. The long-term leaching risk of potentially toxic metals (PTMs) in the derived biochars and the oxidation resistance of the biochars were investigated. The results showed that PTMs in the biochar could transform into more stable and less toxic forms with the elevated pyrolysis temperature. PTMs leaching behavior in the 350 °C biochar was considerably sensitive to the leaching while that in the 750 °C biochar was not affected. The biochar produced at a higher temperature possessed a greater oxidation resistance with little amount of C loss, and the stronger oxidation resistance would prevent the release of metals bound to the organic matter. The findings of this study demonstrated that high-temperature pyrolysis was able to reduce the potential risk of PTMs and ensure carbon stability.

A series of NaY(WO 4 ) 2 :Sm 3+ phosphors were prepared by high temperature solid state reaction. When excited by ultraviolet and blue light, their emission spectra cover entirely visible light region, due to intrinsic luminescence of WO 4 2- group as well as Sm 3+ 4f-4f transitions. White light emission was obtained from NaY 0.99 Sm 0.01 (WO 4 ) 2 phosphor under radiation of 265 nm UV light, and intense yellow and red emission from 6 H J(J=5/2, 7/2, 9/2) transitions were observed when pumped Sm 3+ 4 G 5/2 by 405 nm blue light. With incorporation of Sm 3+ into NaY(WO 4 ) 2 host, higher-level emission from Sm 3+ at 650 nm was generated by energy transfer from WO 4 2- to Sm 3+ under excitation of 265 nm. The corresponding energy transfer mechanism was demonstrated to be a dipole-dipole interaction. In addition, tunable emission from blue to white and, finally, to red was realized by increasing Sm 3+ doping concentration. The band gap of NaY(WO 4 ) 2 calculated from diffuse reflection spectra indicates a semiconducting character. All these results show that NaY 1−x Sm x (WO 4 ) 2 phosphor provides promising application for conversion of frequencies emitted by UV or blue LEDs.

Ethnopharmacological relevance Colla Corii Asini is a widely used traditional Chinese medicine to treat anemia with a long history due to its stimulating effect in hematopoiesis, but the components contributing to this effect are still unknown. In this study, we aimed to establish a methodology to isolate the bioactive components and provide pharmacological basis for its usage in treating anemia. Methods 5-FU and γ-ray radiation induced anemic mice models were generated by treating with 5-FU at 150 mg/kg body weight and γ-rays by a 4 MV linear accelerator by total body irradiation using female ICR mice respectively. Oral administration of fraction A was performed by gastric lavage at 1 g/kg and 2 g/kg body weight for 12 days and 25 days and peripheral blood sample was collected from ocular sinus red blood cell (RBC) and white blood cell (WBC) counts every 3 days and 5 days for 5-FU and radiation induced models, respectively. Next, fraction A was separated to A1 and A2 using cation exchange chromatography (IEC) based on ionic strength. Fraction A1 was further separated using reverse phase chromatography (RPC) based on the hydrophobicity first with 0–10% linear gradient, then 20%, 30%, 50% constant gradient of 60% acetonitrile in neutral Na 2 HPO 4 buffer. Peak fractions were pooled, evaporatively dried, and dissolved in ultrapure water. Finally, fraction A11 was analyzed combining tandem mass spectrometry and proteomic tools and two peptides (peptide 11 and 16) were identified. The hematopoietic effects of multiple fractions and the two peptides were measured using colony-forming units-erythroid (CFU-E), an indication of late erythroid progenitor cells and colony-forming units granulocyte-monocyte (CFU-GM), an indication of granulocyte and monocyte progenitor cells respectively on hematopoietic progenitor cells prepared from bone marrow ( Till and Mcculloch 1961 ). Results Fraction A at 1 g/kg and 2 g/kg could increase RBC and WBC counts in 5-FU and radiation induced anemic mice models. Fraction A1 at 0.1 mg/ml and 0.5 mg/ml, exhibited stronger hematopoietic activity than fraction A2, both of which were subfractions from fraction A using IEX, by elevated CFU-E and CFU-GM of mouse bone marrow cells. Furthermore, fraction A11 at 0.1 mg/ml showed stronger CFU-E and CFU-GM than fractions A12 to A14 from RPC separation. Finally, peptide 11 and peptide 16 were identified from tandem mass spectrometry and peptide 11 increased CFU-E and CFU-GM in a dose dependent manner. Conclusions We combined multiple approaches including chromatography, mass spectrometry, cell-based assays, as well as animal studies to identify and demonstrate that the hematopoietic effect of Colla Corii Asini is at least in part from the peptidic components identified using our methodology. This is the first time to isolate peptidic components from Colla Corii Asini , and to provide molecular basis for its usage in treating anemia, which may particularly have the potential to benefit cancer patients suffering from myelosuppression due to radiotherapy or chemotherapy.

The present work was done to explore the joint effect of Streptomyces pactum (Act12) and plant nutrients on phytoremediation of smelter-contaminated soils. The physiological indicators and phytoextraction indices of potherb mustard (Brassica juncea, Coss) grown in Act12 inoculated soil with or without Hoagland’s solution (H), humic acid (HA) and peat (PS) were evaluated. The results indicated that H, HA and PS acted synergistically with Act12, notably increasing chlorophyll and soluble protein contents and thereby promoting plant growth. Soil nutrient treatments reduced the antioxidant activities (PPO, CAT and POD) by 28.2–41.4%, 22.3–90.1% and 15.2–59.4% compared to control, respectively. Act12 and H treatments markedly facilitated plant to accumulate more cadmium (Cd) and zinc (Zn), but it was observed decreases when applied with HA and PS. Metal uptake (MU) values further indicated the differences in phytoextraction efficiency, i.e., H > PS > Control > HA. Taken together, Act12 combined with plant nutrients contributed to alleviating metal toxicity symptoms of plant. Hoagland’s solution and peat were highlighted in the present phytoextraction trial, and recommended as soil additives.

Of the many parameters that affect the enrichment factors in headspace liquid-phase microextraction, in this study, we systematically investigated the influence of the vapor pressure of the extracting solvent. Seven extracting solvents with different vapor pressures were selected and tested. It was found that the vapor pressure of the extracting solvent dramatically affects the enrichment factor and the factor was increasing by decreasing the extracting solvent vapor pressure under given experimental conditions. The result was validated for volatile organic compounds such as polynuclear aromatic hydrocarbons, organochlorine pesticides and polychlorinated biphenyls.