1. First evidence of DAAM1 localization in mouse seminal vesicles and its possible involvement during regulated exocytosis
- Author
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Francesco Aniello, Chiara Fasano, Sergio Minucci, Alessandra Santillo, Massimo Venditti, Venditti, Massimo, Fasano, Chiara, Santillo, Alessandra, Aniello, Francesco, and Minucci, Sergio
- Subjects
Male ,rho GTP-Binding Proteins ,0301 basic medicine ,Cytoplasm ,RHOA ,macromolecular substances ,Exocytosis ,General Biochemistry, Genetics and Molecular Biology ,Exocytosi ,Mice ,03 medical and health sciences ,Smooth muscle ,Cell polarity ,Cell cortex ,Morphogenesis ,Animals ,Cytoskeleton ,Actin ,DAAM1 ,030102 biochemistry & molecular biology ,General Immunology and Microbiology ,biology ,Chemistry ,Microfilament Proteins ,Cell Polarity ,Seminal Vesicles ,General Medicine ,DAAM1, Actin, Exocytosis, Seminal vesicles, Smooth muscle ,Actins ,Seminal vesicle ,Cell biology ,030104 developmental biology ,Formins ,biology.protein ,General Agricultural and Biological Sciences - Abstract
Dishevelled-associated activator of morphogenesis 1 (DAAM1) is a protein belonging to the formin family, which regulates, together with the small GTPase RhoA, the nucleation and the assembly of actin fibres through Wnt-Dishevelled PCP pathway. Its role has been investigated in essential biological processes, such as cell polarity, movement and adhesion during morphogenesis and organogenesis. In this work, we studied the expression of DAAM1 mRNA and protein by PCR and Western blot analyses and its co-localization with actin in adult mouse seminal vesicles by immunofluorescence. We show that both proteins are cytoplasmic: actin is evident at cell–cell junctions and at cell cortex; DAAM1 had a more diffused localization, but is also prominent at the apical plasmatic membrane of epithelial cells. These findings support our hypothesis of a role of DAAM1 in cytoskeletal rearrangement that occurs during the exocytosis of secretory vesicles, and in particular concerning actin filaments. We were also able to detect DAAM1 and actin association in the smooth muscle cells that surround the epithelium too. In this case, we could only speculate the possible involvement of this formin in muscular cells in the maintenance and the regulation of the contractile structures. The present results strongly suggest that DAAM1 could have a pivotal role in vesicle exocytosis and in the physiology of mouse seminal vesicles.
- Published
- 2018
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