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The psychometric properties of the revised Early Childhood Environment Rating Scale (ECERS–R) were examined using data from the German National Study of Child Care in Early Childhood (NUBBEK). Our findings on the validity of the ECERS–R replicate prior research on the scale’s response process validity, structural validity, and criterion validity. The Partial Credit Model (PCM) identified disorder of rating categories. Factor analyses did not identify a single global factor of quality of child care, but three factors. Regression analyses revealed small effect sizes for predicting child outcomes and small to moderate effect sizes for predicting alternative measures of quality. Implications for the use and revision of the scale and the development of other measures of child care quality are discussed.

The primary goal of this study was the broad assessment and modeling of scientific reasoning in elementary school age. One hundred fifty-five fourth graders were tested on 20 recently developed paper-and-pencil items tapping four different components of scientific reasoning (understanding the nature of science, understanding theories, designing experiments, and interpreting data). As confirmed by Rasch analyses, the scientific reasoning items formed a reliable scale. Model comparisons differentiated scientific reasoning as a separate construct from measures of intelligence and reading skills and revealed discriminant validity. Furthermore, we explored the relationship between scientific reasoning and the postulated prerequisites inhibitory control, spatial abilities and problem-solving skills. As shown by correlation and regression analyses, beside general cognitive abilities (intelligence, reading skills) problem-solving skills and spatial abilities predicted performance in scientific reasoning items and thus contributed to explaining individual differences in elementary school children's scientific reasoning competencies.

International audience; Self-renewal and differentiation of pluripotent murine embryonic stem cells (ESCs) is regulated by extrinsic signaling pathways. It is less clear whether cellular metabolism instructs developmental progression. In an unbiased genome-wide CRISPR/Cas9 screen, we identified components of a conserved amino-acid-sensing pathway as critical drivers of ESC differentiation. Functional analysis revealed that lysosome activity, the Ragulator protein complex, and the tumor-suppressor protein Folliculin enable the Rag GTPases C and D to bind and seclude the bHLH transcription factor Tfe3 in the cytoplasm. In contrast, ectopic nuclear Tfe3 represses specific developmental and metabolic transcriptional programs that are associated with peri-implantation development. We show differentiation-specific and non-canonical regulation of Rag GTPase in ESCs and, importantly, identify point mutations in a Tfe3 domain required for cytoplasmic inactivation as potentially causal for a human developmental disorder. Our work reveals an instructive and biomedically relevant role of metabolic signaling in licensing embryonic cell fate transitions.