Search

Cities and urban processes typically have a negative impact on biodiversity via land cover change, high rates of disturbance and high densities of pest species. Increasingly, however, people are being encouraged and empowered to reduce these impacts through urban restoration and backyard conservation initiatives. Internationally, lizards are a common feature of urban biodiversity, but in New Zealand where many species are threatened, little is known about populations of native skinks and geckos in cities. Yet cities may offer unique opportunities for lizard conservation compared with alternatively modified habitats. To explore the potential of cities for the conservation of lizards, we collated knowledge about the current lizard faunas of six New Zealand cities and developed a list of species that would likely have been present in the locations of these cities prior to human settlement. Comparing the two, we found that, although each of the cities has at least one currently urban-dwelling species, the diversity of lizards in all of the cities has declined dramatically since human colonisation. Patterns of species loss in cities reflect those observed across New Zealand more generally; that is, the loss of large-bodied skinks and geckos, probably resulting from predation by introduced mammalian predators, as well as the loss of regionally endemic species. The high diversity of species that are currently, or were historically, present in the locations of New Zealand cities means that urban restoration involving recovery or reintroduction of populations could have significant benefits for lizard conservation and advocacy.

Neuraxial opioids were first used for labour analgesia in 1980 following the description of spinal cord opioid receptors in 1979. Via these receptors in the dorsal horn, opioids modulate both the visceral and the somatic pain of labour. The onset and duration of action of the individual opioid are determined primarily by its relative lipid solubility. Neuraxial opioids have a local anaesthetic sparing effect, allowing the use of lower concentrations of both agents while maintaining analgesia. As a sole agent, intrathecal opioids can be used to provide analgesia during the first stage of labour, especially in the high-risk parturient. They also have a role in the management of perineal pain and the provision of rapid-onset analgesia. Unfortunately maternal and neonatal side-effects can occur, the most important being respiratory depression.

The first spinal anaesthetic for obstetrics was administered in 1900. Epidural anaesthesia, as a single injection technique, was introduced around the same time, and by the 1950s had become the more popular technique. The use of spinal anaesthesia for Caesarean section has increased since the late 1980s due to the introduction of the pencil-point spinal needles which are associated with a low incidence of headache. Spinal anaesthesia has a number of advantages. It is a reliable, easily learned technique which requires a low dose of local anaesthetic and, hence, has a low risk of toxicity. The quality of the block is better and the onset is more rapid than with epidural anaesthesia. Unlike the epidural, spinal anaesthesia largely remains a single injection technique which has some disadvantages. The side-effect profile of spinal anaesthesia is similar to that seen with epidural anaesthesia. In summary, spinal anaesthesia produces rapid, effective, reliable and safe anaesthesia f or Caesarean section while using low doses of local anaesthetic. This makes it the preferable technique for elective procedures.

Diagnosis of Cytomegalovirus Infections and Development of Monocional Antibodies Cytomegalovirus (CMV) is a common infection that can cause serious disease in infants who have acquired the virus in utero, in patients immunosuppressed for organ transplantation, and in other individuals who receive chemotherapy or high levels of corticosteroids for treatment of neoplastic and other medical processes. Only 4 years ago, the standard method for detecting CMV infection in the laboratory was the inoculation of diploid fibroblast cell cultures in tubes with subsequent identification of typical cytopathic effects that required an average time period of 7 8 days for recognition (3). Immunologic methods for rapid detection of CMV in clinical specimens waned for lack of high quality homotypic antisera that could produce sensitive and specific results and, importantly, be commercially available. Subsequently, two monoclonal antibodies were prepared against an early and a late antigen of the ADI69 strain of CMV by Shuster et al. at the Mayo Clinic. These monoclonal antibodies provided the key to the development of a rapid test for detection of the virus (10). Since 1973, we have used shell vials to diagnose Chlamydia trachomatis infections. This bacterium is .only 3 4 times larger than the CMV virion; nevertheless, centrifugation of the specimen inoculum at low speed (700 x g) has been shown to enhance the rate and ultimate infectivity of this organism and viruses in susceptible cell cultures. Because we wanted to be able to detect CMV as rapidly as possible after inoculation of cell cultures in the laboratory, we used the monoclonal antibody prepared to an early antigen (72,000 d) to assay for the viral components synthesized after 16 hr incubation by the indirect immunofluorescence test (monoclonal antibody available from DuPont Specialty Diagnostics, Wilmington, DE).