1. Cytotoxic cycloartane triterpenoids from the leaves of Markhamia stipulata var. canaense
- Author
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Van Son Dang, Dinh Tri Mai, Trong Nghia Ngo, Tan Phat Nguyen, Nhat Minh Phan, Cong Luan Tran, and Trong Dat Bui
- Subjects
biology ,Traditional medicine ,010405 organic chemistry ,Stereochemistry ,Ethyl acetate ,Plant Science ,biology.organism_classification ,01 natural sciences ,Biochemistry ,Jurkat cells ,0104 chemical sciences ,Markhamia stipulata ,HeLa ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Cell culture ,Cytotoxic T cell ,Cytotoxicity ,Agronomy and Crop Science ,IC50 ,Biotechnology - Abstract
From the most cytotoxic fraction of the ethyl acetate extract of Markhamia stipulata var. canaense V.S. Dang leaves, two new cycloartane-type triterpenoids, named Markhacanasin A (1) and Markhacanasin B (2); were isolated by various chromatographic methods Their structures were elucidated by IR, UV, HR-ESI–MS, NMR (1D & 2D) experiments. The cytotoxicity of two new compounds against five human cancer cell lines (HeLa, HepG2, MCF-7, Jurkat and NCI-H460) were evaluated by SRB assay. As results, 1 exhibited significant cytotoxic activity against all cancer cell lines (IC50 ranged from 14.72 to 29.55 μM) while 2 did not show activity.
- Published
- 2017
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