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Your search keyword '"Duan, Kang"' showing total 35 results
Start Over Author "Duan, Kang" Publisher elsevier bv
35 results on '"Duan, Kang"'

4. Inhibition of Cyclin-Dependent Kinase 8/Cyclin-Dependent Kinase 19 Suppresses Its Pro-Oncogenic Effects in Prostate Cancer

Author

Anne Offermann, Vincent Joerg, Finn Becker, Marie C. Roesch, Duan Kang, Anna-Lena Lemster, Lars Tharun, Jochen Behrends, Axel S. Merseburger, Zoran Culig, Verena Sailer, Johannes Brägelmann, Jutta Kirfel, and Sven Perner

Subjects
Male, Carcinogenesis, Androgens, Humans, Prostatic Neoplasms, Androgen Antagonists, Cyclin-Dependent Kinase 8, Cyclin-Dependent Kinases, Pathology and Forensic Medicine
Abstract

Progression of prostate cancer (PCa) is characterized by metastasis and castration resistance after response to androgen deprivation. Therapeutic options are limited, causing high morbidity and lethality. Recent work reported pro-oncogenic implications of the Mediator subunits cyclin-dependent kinase (CDK) 8 and 19 for the progression of PCa. The current study explored the underlying molecular mechanisms of CDK8/CDK19 and tested effects of novel CDK8/CDK19 inhibitors. PC3, DU145, LNCaP, and androgen-independent LNCaP Abl were used for in vitro experiments. Two inhibitors and CDK19 overexpression were used to modify CDK8/CDK19 activity. MTT assay, propidium iodide staining, wound healing assay, Boyden chamber assay, and adhesion assay were used to investigate cell viability, cell cycle, migration, and adhesion, respectively. Peptide-kinase screen using the PamGene platform was conducted to identify phosphorylated targets. Combining CDK8/CDK19 inhibitors with anti-androgens led to synergistic antiproliferative effects and sensitized androgen-independent cells to bicalutamide. CDK8/CDK19 inhibition resulted in reduced migration and increased collagen I-dependent adhesion. Phosphorylation of multiple peptides linked to cancer progression was identified to be dependent on CDK8/CDK19. In summary, this study substantially supports recent findings on CDK8/CDK19 in PCa progression. These findings contribute to a better understanding of underlying pro-oncogenic effects, which is needed to develop CDK8/CDK19 as a therapeutic target in PCa.

Published
2022

31. Numerical investigation of the direct tensile behaviour of laminated and transversely isotropic rocks containing incipient bedding planes with different strengths

Author

Shang, Junlong, Duan, Kang, Gui, Yilin, Handley, Kirk, Zhao, Zhiye, Shang, Junlong, Duan, Kang, Gui, Yilin, Handley, Kirk, and Zhao, Zhiye

Abstract

The anisotropic direct tensile behaviour of laminated and transversely isotropic Midgley Grit sandstone (MGS) was investigated in this paper using a particle-based discrete element method (DEM) with consideration of the incipiency of bedding planes. Laboratory experiments were conducted to quantify the direct tensile strength of the incipient bedding planes, and the results were used to calibrate the numerical model. The effects of bedding planes with different relative positions and spacing were discussed. A comparison between the simulated results of this study and the predicted results from failure criteria was completed, and a good agreement was observed.

Published
2018

34. Depression of pyrite in alkaline medium and its subsequent activation by copper

Author

Duan Kang, Yuqiong Li, Jianhua Chen, and Jin Guo

Subjects
Mechanical Engineering, Inorganic chemistry, chemistry.chemical_element, General Chemistry, engineering.material, Calcium, Geotechnical Engineering and Engineering Geology, Copper, Ion, chemistry.chemical_compound, Adsorption, chemistry, Dixanthogen, Control and Systems Engineering, engineering, Xanthate, Pyrite, Froth flotation
Abstract

The adsorption of hydroxyl and calcium hydroxyl ions (OH− and CaOH+) on pyrite (1 0 0) surfaces was studied using first-principles calculations to investigate the depression of pyrite by NaOH and CaO. The calculation results showed that the adsorption of CaOH+ on pyrite surfaces was stronger than the adsorption of OH−. The surface Fe atoms were the active sites for the two adsorbates, which is not conducive to the interaction of xanthate with the pyrite surface. In addition, partial surface S atoms were covered by Ca in CaOH+, which would not be favourable for the copper activation of pyrite. The OH− obtained electrons from the surface, whereas CaOH+ lost electrons to the surface. The loss of electrons resulted in the accumulation of electrons on the surface, which hindered the oxidation of pyrite and the formation of dixanthogen. Flotation tests showed that the Cu activation of pyrite after depression by CaO was more difficult than after depression by NaOH. This result was consistent with the calculated result that partial surface S sites, which were the active sites of Cu adsorption, were covered by Ca; this coverage resulted in the Cu activation becoming difficult.

Published
2012

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