1. Brainstem and hypothalamic areas involved in respiratory chemoreflexes: a Fos study in adult rats
- Author
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Nicole Larnicol, Patrick Berquin, L. Bodineau, Françoise Gros, Dumonceaud, Corinne, Neuropeptides centraux et régulations hydrique et cardiovasculaire, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Labex MemoLife, Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Dysrégulations Métaboliques Acquises et Génétiques. Approches moléculaires, physiologiques et neurobiologiques, Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (LBVPAM), Université Jean Monnet [Saint-Étienne] (UJM), Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre interdisciplinaire de recherche en biologie (CIRB), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Labex MemoLife, Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Dysrégulations Métaboliques Acquises et Génétiques. Approches moléculaires, physiologiques et neurobiologiques - EA UPJV 3901 (EA3901), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (BVPAM), and Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Neurons ,MESH: Anoxia ,MESH: Rats, Sprague-Dawley ,Supraoptic nucleus ,MESH: Charcoal ,Hypercapnia ,Rats, Sprague-Dawley ,0302 clinical medicine ,Neural Pathways ,MESH: Renal Dialysis ,MESH: Animals ,MESH: Chemoreceptors ,Hypoxia ,[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Neurons ,0303 health sciences ,MESH: Kidney, Artificial ,MESH: Proto-Oncogene Proteins c-fos ,General Neuroscience ,MESH: Aluminum ,Solitary tract ,Immunohistochemistry ,Chemoreceptor Cells ,medicine.anatomical_structure ,Hypothalamus ,Respiratory Physiological Phenomena ,Brainstem ,Proto-Oncogene Proteins c-fos ,circulatory and respiratory physiology ,medicine.medical_specialty ,MESH: Rats ,Central nervous system ,Biology ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Molecular Biology ,Medulla ,MESH: Respiratory Physiology ,030304 developmental biology ,MESH: Neural Pathways ,MESH: Immunohistochemistry ,MESH: Hypothalamus ,Pons ,Rats ,Endocrinology ,nervous system ,MESH: Hypercapnia ,MESH: Oxides ,MESH: Brain Stem ,Neurology (clinical) ,MESH: Adsorption ,Neuroscience ,Nucleus ,030217 neurology & neurosurgery ,Brain Stem ,Developmental Biology - Abstract
The adaptation to hypoxia and hypercapnia requires the activation of several anatomical structures along the neuraxis. In this study, using Fos immunoreactivity, we sought to map neuronal populations involved in chemoreflex networks activated during the responses to moderate hypoxia (O(2) 11%), and hypercapnia (CO(2) 5%) in the brainstem and the hypothalamus of the rat. In the medulla, hypoxia elicited marked and significant staining in the nucleus of the solitary tract (NTS), and in parapyramidal neurons located near the ventral surface, whereas hypercapnia evoked significantly c-fos only near the ventral surface in paraolivar neurons. In contrast, within pontine and suprapontine structures, both hypoxia and hypercapnia evoked similarly Fos immunoreactivity in the lateral parabrachialis area, the central grey, the caudal hypothalamus (dorsomedial and posterior hypothalamic nuclei), and in a ventro-lateral hypothalamic area, extending from the rostral limit of the mammillary nuclei to the retrochiasmatic area. More rostrally, labelling was observed in the paraventricular nucleus of the hypothalamus in response to hypercapnia, and in the supraoptic nucleus in response to hypoxia. These results support the hypothesis that chemoreflexes pathways are not only restricted to medulla and pons but also involved mesencephalic and hypothalamic regions. The parabrachialis area and the central grey may be key relays between caudal and ventral hypothalamic neurons, and medullary neurons involved in the response to hypoxia and hypercapnia.
- Published
- 2000
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