1. Compensatory induction of Fads1 gene expression in heterozygous Fads2-null mice and by diet with a high n-6/n-3 PUFA ratio
- Author
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Yuan Xiang Pan, Xingguo Wang, Hang Su, Manabu T. Nakamura, and Dan Zhou
- Subjects
Fatty Acid Desaturases ,0301 basic medicine ,fatty acid/desaturases ,030204 cardiovascular system & hematology ,Biochemistry ,Mice ,chemistry.chemical_compound ,Delta-5 Fatty Acid Desaturase ,0302 clinical medicine ,Endocrinology ,brain lipids ,heterocyclic compounds ,FADS1 Gene ,Fatty Acid Desaturase 1 ,Research Articles ,Mice, Knockout ,chemistry.chemical_classification ,Arachidonic Acid ,biology ,food and beverages ,Fatty Acids, Unsaturated ,lipids (amino acids, peptides, and proteins) ,Arachidonic acid ,diet and dietary lipids ,Polyunsaturated fatty acid ,Heterozygote ,medicine.medical_specialty ,animal structures ,Docosahexaenoic Acids ,Genotype ,FADS2 ,QD415-436 ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Internal medicine ,Fatty Acids, Omega-3 ,medicine ,Animals ,Humans ,RNA, Messenger ,adipocyte protein 2 ,fatty acid/elongases ,Fatty acid ,Cell Biology ,cytokines ,Diet ,030104 developmental biology ,Fatty acid desaturase ,chemistry ,inflammation ,biology.protein - Abstract
In mammals, because they share a single synthetic pathway, n-6/n-3 ratios of dietary PUFAs impact tissue arachidonic acid (ARA) and DHA content. Likewise, SNPs in the human fatty acid desaturase (FADS) gene cluster impact tissue ARA and DHA. Here we tested the feasibility of using heterozygous Fads2-null-mice (HET) as an animal model of human FADS polymorphisms. WT and HET mice were fed diets with linoleate/α-linolenate ratios of 1:1, 7:1, and 44:1 at 7% of diet. In WT liver, ARA and DHA in phospholipids varied >2× among dietary groups, reflecting precursor ratios. Unexpectedly, ARA content was only
- Published
- 2016
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