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This article contains raw and processed data related to research published in “Role of the polypeptide N-acetylgalactosaminyltransferase 3 in ovarian cancer progression: possible implications in abnormal mucin O-glycosylation” [1]. The data presented here was obtained with the application of a bioorthogonal chemical reporter strategy analyzing differential glycoprotein expression following the knock-down (KD) of the GALNT3 gene in the epithelial ovarian cancer (EOC) cell line A2780s. LC-MS/MS mass spectrometry analysis was then performed and the processed data related to the identified glycoproteins show that several hundred proteins are differentially expressed between control and GALNT3 KD A2780s cells. The obtained data also uncover numerous novel glycoproteins; some of which could represent new potential EOC biomarkers and/or therapeutic targets. Keywords: GALNT3, Glycosylation, Ac4GalNAz labeling, Label free quantification, NetOGlyc and NetNGlyc prediction analysis, Glycoproteomics

Hybrid materials based on one organic dye, the carminic acid, and montmorillonite have been prepared and characterized by a multi-technical approach. Aluminum and tin II cations were used in the experimental mixtures in order to have a chelating effect with the carminic acid. X-Ray diffraction patterns (XRD) did not highlight any intercalation of the organic molecule in the interlayer space, a result confirmed by transmission electron microscopy (TEM) micrographs. The fixation of the organic molecules on the phyllosilicates was then investigated by 13 C solid state nuclear magnetic resonance (NMR) and time resolved fluorescence spectroscopies. The spectra showed the role that the ketone and catechol functions of the dye played for its fixation and stressed on the decrease of its mobility when the lake pigment was formed. The role of the clay edges and/or surface was highlighted by this set of experimental data.

Methylene is found in the repeat units of many polymers including proteins. In some cases it appears to be a useful reporter of variation in local environment whilst in other contexts average behaviour seems to dominate. In this paper we apply a particular 2DIR technique to a range of systems containing methylene groups, showing that mode frequencies, linewidths and splittings can be easily extracted even when the infrared absorption bands are too congested to allow reliable analysis. 2DIR spectra of polyethylene and several liquid alkanes are compared and it is shown for the case of l -arginine that the methylene scissor modes are split and that this can be resolved by tracking the 2DIR spectrum as a function of time. Calculations from first principles reveal that for most of the methylene modes studied, electrical anharmonicity is the dominant contributor to the 2DIR cross-peak intensity, with the mechanical anharmonicity making only a small contribution.

Femtosecond visible pulses at 800 nm are observed to induce permanent spectral changes in the CO internal stretch region of CO/Pt(1 1 1) at 300 K, using sum frequency generation vibrational spectroscopy. By contrast, IR femtosecond, and visible picosecond pulses do not induce any change. Defects, disordering, water coadsorption or laser induced CO dissociation can be ruled out to account for the observed changes, which seem to be due to the formation of a metastable structure. With this structure, the CO coverage seems to increase reversibly in equilibrium with gas phase CO, which normally occurs on various surfaces, but not on Pt(1 1 1). An unusually high frequency for linearly bonded CO at 300 K is obtained above ≈10 −7 mbar. More experimental work using other techniques is required to confirm the occurrence of a metastable structure and to characterize it. Nevertheless the available spectroscopic and structural data concerning CO on Pt(1 1 1) do not support an interpretation where the laser induced metastable structure would remain close to the regular c(4×2) structure.

Second harmonic non-linear susceptibilities of Pd(1 1 1) are derived at 800 nm fundamental wavelength from measurements of second harmonic generation (SHG) as a function of azimuth and input and output polarizations. By contrast to other metal surfaces and to other wavelengths, Pd(1 1 1) does not exhibit any anisotropy related to azimuth at 800 nm. SHG is also measured during CO adsorption at 1064 and 800 nm. Opposite trends are observed at the two wavelengths. The results are discussed in the light of previously reported experiments on Pd(1 1 1) at other wavelengths and on other metals. At 800 nm, free electrons and/or the Shockley surface state probably dominate the SHG response of Pd(1 1 1). CO chemisorption probably reduces the density of the d-band states probed at 1064 nm, and on the contrary enhances the density of the states probed at 800 nm.