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22 results on '"Gerrard J"'

2. Complementation of Der p 2–induced histamine release from human basophils sensitized with monoclonal IgE: Not only by IgE, but also by IgG antibodies directed to a nonoverlapping epitope of Der p 2☆☆☆★★★

Author

Geoffrey A. Mueller, Janine Schuurman, Gerrard J. Perdok, and Rob C. Aalberse

Subjects
Allergy, Recombinant Fusion Proteins, Immunology, Biology, medicine.disease_cause, Immunoglobulin E, Histamine Release, Polymerase Chain Reaction, Epitope, Epitopes, Mice, chemistry.chemical_compound, Allergen, medicine, Animals, Humans, Immunology and Allergy, Antigens, Dermatophagoides, Cloning, Molecular, Anaphylaxis, Sensitization, Glycoproteins, Antibodies, Monoclonal, medicine.disease, Basophils, Mutagenesis, Insertional, Basophil activation, medicine.anatomical_structure, chemistry, Immunoglobulin G, biology.protein, Antibody, Immunoglobulin Heavy Chains, Histamine
Abstract

The interaction of free allergen with two (or more) IgE molecules bound to the high-affinity receptor for IgE (FcϵRI) on mast cells and basophilic granulocytes results in the release of inflammatory mediators. The role of allergen-specific IgG antibodies in the allergic reaction in human beings is less clear. We produced two chimeric IgE antibodies, hIgE-Dp2A and hIgE-Dp2B, directed to two nonoverlapping epitopes (A and B) of the house dust mite allergen Der p 2. Chimeric IgG1 and IgG4 variants of these antibodies were produced also. Basophil activation by the house dust mite allergen Der p 2 was induced after sensitization of basophils with a mixture of chimeric hIgE-Dp2A and hIgE-Dp2B antibodies but not after sensitization by the individual IgE antibodies alone. Basophil activation was also shown after sensitization with hIgE-Dp2A and stimulation with Der p 2 incubated with hIgG1-Dp2B or hIgG4-Dp2B antibodies. Both IgE and IgG antibodies directed to the other nonoverlapping epitope complemented the sensitization by the hIgE-Dp2A antibody. Nonsensitized basophils were not activated by the Der p 2/hIgG-Dp2 mixtures. These results indicate that allergen-specific IgG can complement an IgE-dependent reaction and therefore under certain conditions can act as an anaphylactic antibody. (J Allergy Clin Immunol 1998;101:404-9.)

Published
1998
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10. Effect of age and state of wakefulness on transcutaneous oxygen vlaues in preterm infants: A longitudinal study

Author

Hendrik Hak, Henry Levison, F. John McLaughlin, Gerrard J. Canny, Marjeta Pintar, and Jacqueline Y. Q. Mok

Subjects
Male, Aging, Longitudinal study, business.industry, Infant, Newborn, Infant, Low Birth Weight, Reference Values, Anesthesia, Pediatrics, Perinatology and Child Health, Humans, Medicine, Female, Wakefulness, Longitudinal Studies, Transcutaneous oxygen, business, Blood Gas Monitoring, Transcutaneous, Infant, Premature
Published
1988
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22. Preliminary Communication

Author

Bickel H, Gerrard J, and Hickmans Em

Subjects
medicine.medical_specialty, Endocrinology, Phenylketonurias, Phenylalanine intake, business.industry, Internal medicine, medicine, Phenylalanine, General Medicine, business, Phenylalanine metabolism
Published
1953
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