1. Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu
- Author
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Jie Bai, Xiaojing Wang, Ayixiaguli Bakasi, Caixia Dong, Kai Zhou, and Hailiqian Taoerdahong
- Subjects
Magnetic Resonance Spectroscopy ,Cell Survival ,02 engineering and technology ,Xylose ,Nitric Oxide ,Polysaccharide ,Methylation ,Biochemistry ,Pyrus ,Gel permeation chromatography ,Mice ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,Polysaccharides ,Structural Biology ,Animals ,Immunologic Factors ,Viability assay ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Molecular Structure ,biology ,Plant Extracts ,Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Molecular Weight ,Xyloglucan ,RAW 264.7 Cells ,Pyrus × sinkiangensis ,Cytokines ,Cytokine secretion ,ARAF ,0210 nano-technology - Abstract
Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu. by using the combined techniques of ion-exchange and gel permeation chromatography. According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan. PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues. PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL. It was suggested that PSNP-1 and PSNP-2 may increase macrophage-mediated immunostimulatory activity.
- Published
- 2020
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