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Your search keyword '"Hattori, Masa-Aki"' showing total 19 results
Start Over Author "Hattori, Masa-Aki" Publisher elsevier bv
19 results on '"Hattori, Masa-Aki"'

1. Improvement of the cellular quality of cryopreserved bovine blastocysts accompanied by enhancement of the ATP-binding cassette sub-family B member 1 expression

Author

Mori, Miyuki, primary, Kasa, Shojiro, additional, Isozaki, Yoshihiro, additional, Kamori, Tsugumitsu, additional, Yamaguchi, Shoichiro, additional, Ueda, Shuji, additional, Kuwano, Toshio, additional, Eguchi, Minako, additional, Isayama, Keishiro, additional, Nishimura, Shotaro, additional, Tabata, Shoji, additional, Yamauchi, Nobuhiko, additional, and Hattori, Masa-aki, additional

Published
2013
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16. Lutropin molecules synthesized by rat gonadotroph primary cultures in the presence of swainsonine: their oligosaccharides and biological potencies

Author

Hattori Masa-aki, Wakabayashi Katsumi, and Ozawa Kazunori

Subjects
Male, Time Factors, Wheat Germ Agglutinins, Oligosaccharides, Biochemistry, chemistry.chemical_compound, Alkaloids, Endocrinology, Pituitary Gland, Anterior, Lectins, Acetylglucosaminidase, Mannosidases, Animals, Testosterone, Molecular Biology, Cells, Cultured, chemistry.chemical_classification, Dose-Response Relationship, Drug, biology, Swainsonine, Sepharose, Leydig Cells, Lectin, Rats, Inbred Strains, Luteinizing Hormone, Oligosaccharide, In vitro, Wheat germ agglutinin, Rats, Sialic acid, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, chemistry, Concanavalin A, biology.protein, Plant Lectins, Luteinizing hormone, Pituitary Hormone-Releasing Hormones
Abstract

The biosynthesis of lutropin (luteinizing hormone, LH) containing abnormally processed oligosaccharides has been studied in rat gonadotroph-enriched primary culture in the presence of swainsonine, an inhibitor of the N-linked oligosaccharide processing in the Golgi complex. The inhibitor led to time- and dose-dependent accumulation of highly alkaline LH molecules (pI 9.6, 10.0) with high binding affinity for concanavalin A (ConA), and concomitant decreases of less alkaline components (pI 8.0, 8.5, 8.8) with less affinity for the lectin. The accumulation of LH molecules was much amplified after gonadotrophin-releasing hormone (GnRH) stimulation. The characterization of the oligosaccharide structure performed by means of enzyme treatment and serial chromatography on immobilized ConA, Ricinin communis agglutinin-120 (RCA-120), and wheat germ agglutinin (WGA) showed that swainsonine-induced LH contained principally the hybrid-type oligosaccharide, which was processed by the addition of terminal sialic acid residues on one branch of each N-linked oligosaccharide in both subunits. Small amounts of LH molecules containing asialo or partially sialylated hybrid oligosaccharides were also synthesized. The data for in vitro steroidogenic activities of swainsonine-induced LH indicated that terminal sialic acid on one branch of the hybrid-type oligosaccharides decreased the full agonist activity by approximately 50%.

Published
1988

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