Search

Your search keyword '"Ho, Chai-Ling"' showing total 32 results
Start Over Author "Ho, Chai-Ling" Publisher elsevier bv
32 results on '"Ho, Chai-Ling"'

5. RNA-sequencing of methyl-jasmonate treated turmeric (Curcuma longa) reveals novel protease inhibitor transcripts

Author

Noor Azmi Shaharuddin, Norliza Abu Bakar, Ho Chai-Ling, Maziah Mahmood, and Seow-Neng Chan

Subjects
0106 biological sciences, Candidate gene, Methyl jasmonate, Protease, medicine.medical_treatment, fungi, Plant Science, Computational biology, Biology, biology.organism_classification, 01 natural sciences, 0104 chemical sciences, Transcriptome, 010404 medicinal & biomolecular chemistry, chemistry.chemical_compound, chemistry, Plant defense against herbivory, medicine, Curcuma, KEGG, Gene, 010606 plant biology & botany
Abstract

Turmeric (Curcuma longa) has long been known in Southeast Asia as a medicinal plant and been used as folk remedies to treat minor illnesses like diarrhea or skin diseases. Recent studies on turmeric have shown numerous pharmacological properties such as anti-oxidant, anti-inflammatory, anti-pathogenic including anti-viral protease activities. All these beneficial properties make turmeric a suitable candidate for the discovery of novel protease inhibitors (PIs). PIs are commonly found in all organisms to regulate biological processes. In plant, PIs are reported to play important roles in plant defense mechanism. A number of these PIs have been genetically engineered into crop plants to enhance protection against microorganism and pest insects. However over time, the pathogens and pest insects are slowly adapting to the current strategies and overcome the additional defense barrier. Hence, this study was conducted to identify novel PIs genes from methyl jasmonate (MeJA)-treated turmeric plants through the whole transcriptome sequencing approach. From the raw data reads obtained from the RNA-sequencing of MeJA-treated leaf tissues, a single reference transcriptome was assembled de novo using Trinity software. A total of 105,529 contiguous sequences were obtained. Sequence annotation and homology search were performed onto several protein databases such as Uniprot, Pfam, GO and KEGG which resulted in around 50% of the transcripts showed similarity hits to known proteins. The individual expression profile of the transcripts from the control and MeJA-treated turmeric samples were generated and compared in order to identify differential expressed genes. A total of 4274 transcripts had been identified to be differentially expressed where there were 1599 upregulated transcripts and 2715 downregulated transcripts. A total of 21 transcripts showed sequence similarity hits to PIs families and three of the transcripts were identified to be upregulated from the MeJA treatment. These identified PIs transcript can serve as candidate genes for further functional studies and applications.

Published
2019

8. pH affects growth, physiology and agar properties of agarophyte Gracilaria changii (Rhodophyta) under low light intensity from Morib, Malaysia

Author

Lee, Wei-Kang, Lim, Yi Yi, Ho, Chai Ling, Lee, Wei-Kang, Lim, Yi Yi, and Ho, Chai Ling

Abstract

Changes in coastal water pH alter inorganic carbon chemistry and impose abiotic stress on photosynthetic marine organisms. The red algal cell wall contains sulfated agar which protects them against environmental stresses. In this study, we investigated the effects of three different pHs (6.61, 8.04 and 9.30) on Gracilaria changii cultured in artificial seawater for 3 and 6 days, respectively. The growth rate of G. changii was the highest and the lowest at pH 6.61 and pH 9.30, respectively. Partial thallus degradation was observed in seaweeds treated at pH 9.30. Upon a 3-day treatment, the levels of allophycocyanin, total phycobilins in G. changii cultured at pH 6.61, and all photosynthetic pigments in G. changii cultured at pH 9.30, were significantly lower than those cultured at pH 8.04. G. changii exposed to pH 9.30 for 6 days also had significantly lower levels of chlorophyll a and allophycocyanin than those treated at pH 8.04. A six-day treatment at pH 6.61 caused a decline in the content of chlorophyll a and carotenoids, but an increase in the levels of phycoerythrin, phycocyanin, and total phycobilins, compared to those treated at pH 8.04. G. changii samples treated at pH 6.61 and pH 9.30 have a higher agar content compared to those cultured at 8.04. Gel strength was significantly lower in seaweed cultured at pH 9.30, compared to those cultured at pH 8.04. Gelling temperature and 3,6-anhydrogalactose content of agar were significantly affected by different pHs, but no significant changes were found in the melting temperature, gel syneresis and sulfate content of agar upon treatments. These information enhance our knowledge on physiological response and agar production in G. changii at different pHs, and useful for optimization of seaweed cultivation system in future.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results