1. Biochemical and physiological studies on cells isolated from the medullary thick ascending limb of Henle's loop
- Author
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Rolf K. H. Kinne, Jill Eveloff, Eckehard Bayerdörffer, and Winfried Haase
- Subjects
Calcitonin ,medicine.medical_specialty ,Biological Transport, Active ,Adenylate kinase ,chemistry.chemical_element ,In Vitro Techniques ,Biology ,Biochemistry ,Oxygen ,Cyclase ,Ouabain ,Oxygen Consumption ,Internal medicine ,Renal medulla ,medicine ,Animals ,Kidney Medulla ,Cell Membrane ,Sodium ,Furosemide ,gamma-Glutamyltransferase ,Alkaline Phosphatase ,Molecular biology ,Arginine Vasopressin ,Enzyme Activation ,Kinetics ,Glucose ,Kidney Tubules ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Loop of Henle ,Alkaline phosphatase ,Rabbits ,Sodium-Potassium-Exchanging ATPase ,Bumetanide ,Adenylyl Cyclases ,medicine.drug - Abstract
1. 1. Viable cells were isolated from the thick ascending limb of Henle's loop (TALH) of rabbit renal medulla. 2. 2. The TALH cells were characterized by high activities of calcitonin-stimulated adenylate cyclase and Na-K-ATPase and low activities of alkaline phosphatase, γ-glutamyl-transpeptidase and ADH-sensitive adenylate cyclase. 3. 3. The oxygen consumption of the TALH cells was inhibitable in a dose dependent manner by furosemide and bumetanide. 4. 4. Ouabain (10 −4 M) also inhibited oxygen consumption as did replacement of either Na or Cl in the incubation medium. 5. 5. Preliminary experiments with plasma membrane vesicles prepared from the TALH cells indicated the presence of a coupled NaCl transport system.
- Published
- 1980
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