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Timely diagnosis and identification of etiology of pediatric mild-to-moderate sensorineural hearing loss (SNHL) are both medically and socioeconomically important. However, the exact etiologic spectrum remains uncertain. We aimed to establish a genetic etiological spectrum, including copy-number variations (CNVs) and efficient genetic testing pipeline, of this defect. A cohort of prospectively recruited pediatric patients with mild-to-moderate nonsyndromic SNHL from 2014 through 2018 (n = 110) was established. Exome sequencing, multiplex ligation-dependent probe amplification (MLPA), and nested customized polymerase chain reaction (PCR) for exclusion of a pseudogene, STRCP, from a subset (n = 83) of the cohort, were performed. Semen analysis was also performed to determine infertility (n = 2). Genetic etiology was confirmed in nearly two-thirds (52/83 = 62.7%) of subjects, with STRC-related deafness (n = 29, 34.9%) being the most prevalent, followed by MPZL2-related deafness (n = 9, 10.8%). This strikingly high proportion of Mendelian genetic contribution was due particularly to the frequent detection of CNVs involving STRC in one-third (27/83) of our subjects. We also questioned the association of homozygous continuous gene deletion of STRC and CATSPER2 with deafness–infertility syndrome (MIM61102). Approximately two-thirds of sporadic pediatric mild-to-moderate SNHL have a clear Mendelian genetic etiology, and one-third is associated with CNVs involving STRC. Based on this, we propose a new guideline for molecular diagnosis of these children.

Malaria is a life-threatening infectious disease caused by parasites of the genus Plasmodium. Understanding the biological features of various parasite forms is important for the optical diagnosis and defining pathological states, which are often constrained by the lack of ambient visualization approaches. Here, we employ a label-free tomographic technique to visualize the host red blood cell (RBC) remodeling process and quantify changes in biochemical properties arising from parasitization. Through this, we provide a quantitative body of information pertaining to the influence of host cell environment on growth, survival, and replication of P. falciparum and P. vivax in their respective host cells: mature erythrocytes and young reticulocytes. These exquisite three-dimensional measurements of infected red cells demonstrats the potential of evolving 3D imaging to advance our understanding of Plasmodium biology and host-parasite interactions.

We investigated the effect of asenapine, a commonly used atypical antipsychotic, on voltage-dependent K

Imported malaria due to Plasmodium ovale curtisi and P. ovale wallikeri infections from African countries has increased recently (2011-2014) in Chinese travelers. We report novel genotypes, their prevalence and the predominant haplotypes of P. ovale curtisi and P. ovale wallikeri circumsporozoite protein (CSP) from 20 African countries in Chinese travelers. These genotypes should be considered while designing a CSP-based vaccine against P. ovale malaria.

Aims We investigated the changes in large-conductance Ca2+-activated K+ (BKCa) channels from human umbilical arterial smooth muscle cells experiencing gestational diabetes mellitus (GDM). Main methods Whole-cell patch-clamp technique, arterial tone measurement, RT-PCR, Quantitative real-time PCR, western blot were performed in human umbilical arterial smooth muscle cells. Key findings Whole-cell BKCa current density was decreased in the GDM group compared with the normal group. The vasorelaxant effects of the synthetic BKCa channel activator NS-1619 (10 μM) were impaired in the GDM group compared with the normal group. Reverse-transcription polymerase chain reaction (RT-PCR), real-time RT-PCR, and western blot analyses suggested that the mRNA, total RNA, and protein expression levels of the BKCa channel were decreased in the GDM group relative to the normal group. In addition, the expression levels of protein kinase A and protein kinase G, which regulate BKCa channel activity, remained unchanged between the groups. Applying the BKCa channel inhibitor paxilline (10 μM) induced vasoconstriction and membrane depolarization of isolated umbilical arteries in the normal group but showed less of an effect on umbilical arteries in the GDM group. Significance Our results demonstrate for the first time impaired BKCa current and BKCa channel-induced vasorelaxation activities that were not caused by impaired BKCa channel-regulated protein kinases, but by decreased expression of the BKCa channels, in the umbilical arteries of GDM patients.

Aims The main action of proton pump inhibitors (PPIs) is to inhibit gastric acid secretion, and PPIs are widely used to treat gastric ulcer (GU). However, if the action of promoting gastric mucosal regeneration is added, the effectiveness of GU treatment can be enhanced. Thus, in order to improve the therapeutic effect on GU, we tried to develop combination therapy promoting regeneration in injured tissue besides suppressing gastric acid secretion. Main methods Polydeoxyribonucleotide (PDRN) was selected to evaluate tissue regeneration, and pantoprazole was chosen as one of the PPIs. GU was induced by oral administration of indomethacin once a day for 7 days. Rats in drug-administered groups were intraperitoneally injected with 100 μL normal saline, containing each drug at the indicated concentration, once a day for 14 days after inducing GU. Key findings PDRN and PPI combination therapy potently improved tissue regeneration and inhibited production of pro-inflammatory cytokines. PDRN treatment with or without PPI increased the concentration of cyclic adenosine-3,5′-monophosphate (cAMP) and the ratio of phosphorylated cAMP response element-binding protein (p-CREB) to cAMP response element-binding protein (CREB). PDRN treatment with or without PPI also increased the expressions of vascular endothelial growth factor (VEGF) and adenosine A2A receptor. Significance PDRN and PPI combination therapy showed more potent therapeutic effect on GU compared to the PDRN monotherapy or PPI monotherapy. The excellent therapeutic effect of PDRN and PPI combination therapy on GU appeared by promoting regeneration of damaged tissue as well as inhibiting gastric acid secretion.

A sparse population of neurons active during a learning event has been identified as memory engram cells. However, cells that are recruited to support memory when experience is repeated have been scarcely explored. Evidence from previous studies provides contradictory views. To address these questions, we employed learning-dependent cell labeling in the lateral amygdala (LA) and applied electrophysiological recording, spine imaging, and optogenetic tools to the labeled neurons with or without retraining. We found that engram cells established from original fear learning became dispensable for memory retrieval specifically with relearning, and this correlated with a reduction of synaptic transmission and loss of dendritic spines in these neurons. Despite such decreased connectivity, direct activation of these neurons resulted in fear-memory recall. We further identified that repeated memory was encoded in neurons active during relearning. These results suggest a shift in neuronal ensembles encoding fear memory in the LA by relearning through disconnection of the existing engram neurons established from original experience.

Aims Canagliflozin is an anti-diabetic agent and sodium glucose co-transporter-2 inhibitor. Despite numerous clinical trials demonstrating its beneficial effects on blood pressure, the cellular mechanisms underlying the effects of canagliflozin on vascular reactivity have yet to be clarified. We investigated the vasodilatory effect of canagliflozin on aortic rings isolated from rabbits. Main methods We used rabbit thoracic aortic rings and its arterial tone was tested by using wire myography system. Key findings Canagliflozin caused concentration-dependent vasodilation in aortic rings pre-constricted with phenylephrine or high K+. However, the degree of canagliflozin-induced vasodilation of the aortic rings pre-constricted with high K+ was less than that of rings pre-constricted with phenylephrine. Application of 4-aminopyridine, a voltage-dependent K+ (Kv) channel inhibitor, reduced canagliflozin-induced vasodilation. However, pre-incubation of an inwardly rectifying K+ channel inhibitor, a large-conductance Ca2+-activated K+ channel inhibitor, and an ATP-sensitive K+ inhibitor did not modulate the vasodilatory effects of canagliflozin. Indeed, canagliflozin increased Kv currents in aortic smooth muscle cells. Pre-treatment with thapsigargin or cyclopiazonic acid, a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump inhibitors, reduced the vasodilatory effects of canagliflozin. Conversely, pre-treatment with a Ca2+ channel inhibitor, adenylyl cyclase/PKA inhibitors, and guanylyl cyclase/PKG inhibitors did not modulate the vasodilatory effects of canagliflozin. Endothelium removal, and pre-treatment with the nitric oxide synthase inhibitor L-NAME, and small- and intermediate-conductance Ca2+-activated K+ channel inhibitor apamin and TRAM-34, did not diminish the vasodilatory effects of canagliflozin. Significance Our results indicate that canagliflozin induces vasodilation, which is dependent on the robust SERCA activity and Kv channel activation.

The dentate gyrus (DG) of the hippocampus is essential for contextual and spatial memory processing. While lesion or silencing of the DG impairs contextual memory encoding and recall, overly activated DG also prevents proper memory retrieval. Abnormally elevated activity in the DG is repeatedly reported in amnesic mild cognitive impairment (aMCI) patients or aged adults. Although the correlation between memory failure and abnormally active hippocampus is clear, their causal relationship or the underlying nature of such interfering activity is not well understood. Using optogenetics aided by a carefully controlled adeno-associated virus infection system, we were able to examine the differential effects of abnormally activated hippocampus on mice motor behavior and memory function, depending on the extent of the stimulation. Optogenetic stimulation of massive proportion of dorsal DG cells resulted in memory retrieval impairment, but also induced increase in general locomotion. Random additional activity in a sparse population of dorsal DG neurons, however, interfered with contextual memory recall without inducing hyperactivity. Our findings thus establish the causal role of elevated DG activity on memory recall failure, suggesting such aberrant DG activity may contribute to amnesic symptoms in aMCI patients and aged adults.

The CT finding of "vulnerable plaque" is widely regarded as similar to that of a culprit lesion in an acute coronary syndrome (ACS). However, this hypothesis may not be accurate, since "vulnerable plaques" may substantially change their morphology when they rupture to cause an ACS.We retrospectively evaluated coronary CT angiography data sets of 25 patients with ACS who had vulnerable (n = 10) or culprit plaques (n = 15). We analyzed CT features including positive remodeling (PR), low attenuation plaque (LAP), the napkin ring sign (NRS), degree of stenosis (normal,50%, 50-99%, 100%), and myocardial hypoperfusion in the left ventricle.There was no difference in the prevalence of PR, NRS, or LAP between vulnerable and culprit plaques. In contrast, a majority (80%, 8/10) of vulnerable plaques were associated with50% luminal stenosis while total occlusion was identified in 47% (7/15) of culprit plaques (p = .037). In all patients with occlusion, myocardial hypoperfusion was demonstrated in the corresponding arterial territory on CT.CT features of vulnerable and culprit plaques differ in cases with thrombotic occlusion reflecting dynamic plaque changes related to the episode of ACS.

Objective Sleep problems are common among patients with attention-deficit hyperactivity disorder (ADHD), and are considered major causes of behavioral and cognitive dysfunction in ADHD patients. In the present study, we investigated the relationship between sleep parameters and cognitive function in drug-naive children with ADHD. Methods Twenty-eight patients were recruited to participate in the study, and a polysomnography was used to measure sleep parameters of the subjects. Cognitive measurements were collected, utilizing the Wechsler Intelligence Scale for Children-III (WISC-III), and the Matching Familiar Figure Test for Korean Children (MFFT-KC), while behavioral characteristics of the subjects were assessed using Conners' Global Index-Parent version (CGI-P). Descriptive statistics were calculated for demographic data, sleep parameters, and neurocognitive characteristics of ADHD patients. Spearman's correlation analyses were performed to determine the association between sleep parameters and neurocognitive measures. Moreover, multiple regression analyses were used to identify the best predictors of cognitive function among the various sleep parameters. Results The regression analyses revealed several meaningful correlations, suggesting that slow wave sleep, stage 2 sleep, REM sleep, and limb movement index with arousals (LMAs) as predictors of cognitive function in ADHD patients. Conclusion Based on our study results, sleep parameters and cognitive function were closely associated in ADHD patients; further research should be directed at clarifying this crucial link.

One of the challenges in shrinking immunoassays to smaller sizes is to immobilize the biological molecules to nanometer-scaled spots. To overcome this complication, we have employed a particle-based immunoassay to create a nanostructured platform with a regular array of sensing elements. The technique makes use of an electrophoretic particle entrapment system (EPES) to immobilize nanoparticles that are coated with biological reagents into wells using a very small trapping potential. To provide useful information for controlling the trapping force and optimal design of the nanoarray, electrophoretic trapping of a nanoparticle was modeled numerically. The trapping efficiency, defined as the fraction of wells occupied by a single particle, was 91%. The performance of the array was demonstrated with a competitive immunoassay for a small molecule analyte, 3-phenoxybenzoic acid (214.2 g mole(-1)). The limit of detection determined with a basic fluorescence microscope was 0.006 μg l(-1) (30 pM); this represented a sixteen-fold improvement in sensitivity compared to a standard 96-well plate-based ELISA; the improvement was attributed to the small size of the sample volume and the presence of light diffraction among factors unique to this structure. The EPES/nanoarray system promises to offer a new standard in applications that require portable, point-of-care and real-time monitoring with high sensitivity.

Adsorption and desorption of fullerene on a single layer of graphene grown on SiC(0001) were investigated by photoemission spectroscopy (PES). No significant change in the band structure of graphene was observed after fullerene deposition on the graphene layer under vacuum conditions, and subsequent exposure to the air. After annealing the fullerene layer at 275 °C in a vacuum, complete desorption of fullerene was observed without any resulting damage to the graphene structure. The desorption temperature of fullerene was significantly higher than that of pentacene, indicating that fullerene layers show higher stability than pentacene as protection layers of graphene-based devices.

An environmental prediction model was developed for optimal ventilation in a mushroom house utilising a sensible heat balance and a three-dimensional (3-D) computational fluid dynamics (CFD) model. The respiration of the mushrooms and the use of a low-capacity cooler were considered. A mushroom-house-specific ventilation equation was developed to calculate the ventilation rate for a given environmental condition. Calculated ventilation rates were compared with the experimentally measured data for the indoor temperature set to the optimum for growing mushrooms (16.2 °C) with varying outdoor temperature. There was good agreement between the measured and predicted rates (0.2–5.1% error). Calculated ventilation rates (from the sensible heat balance) were used as an input parameter for 3-D CFD model, eliminating the need for experimental measurement of ventilation rate. 3-D CFD simulations were conducted using the same environmental condition to establish the local heat distribution in a mushroom house. The simulation results for temperature were compared with the experimental data at several different locations in a mushroom house and showed negligible errors. The CFD model was also used to improve heat distribution of a mushroom house. It was predicted that enhanced cooling and more uniform temperature distribution could be achieved just by changing the direction of airflow from air inlet ducts and/or installing small fans onto them, but not by changing the directions of airflow from a cooler. This would be a more economical than replacing a cooler or redesigning the entire structure. The model could be used to predict the environmental conditions over different locations in a mushroom house without the need for experimentally determining the ventilation rate.

The distribution of humidity in a greenhouse was studied using three-dimensional (3-D) computational fluid dynamics (CFD). The calculations were validated using experimental data from a single-span greenhouse without plants. Two types of humidity distribution were considered: humidifying using a fog cooling system, and dehumidifying using refrigerative dehumidifiers in addition to a fog cooling system. The simulation errors of RH were 0.1–18.4% with a fog cooling system and 1.1–13.1% with a fog cooling system and refrigerative dehumidifiers at each observation point. Contour maps were obtained from the 3-D CFD simulations to locate any non-uniformity in humidity distribution. The use of refrigerative dehumidifiers reduced the overall difference of humidity between the middle and bottom zones of a greenhouse, but the local distribution of humidity was uneven, especially close to the dehumidifiers. This study suggests that the developed 3-D CFD model can be a useful tool in designing and evaluating greenhouses with various configurations.

SummaryRepeated pulses of serotonin (5-HT) induce long-term facilitation (LTF) of the synapses between sensory and motor neurons of the gill-withdrawal reflex in Aplysia. To explore how apCAM downregulation at the plasma membrane and CREB-mediated transcription in the nucleus, both of which are required for the formation of LTF, might relate to each other, we cloned an apCAM-associated protein (CAMAP) by yeast two-hybrid screening. We found that 5-HT signaling at the synapse activates PKA which in turn phosphorylates CAMAP to induce the dissociation of CAMAP from apCAM and the subsequent translocation of CAMAP into the nucleus of sensory neurons. In the nucleus, CAMAP acts as a transcriptional coactivator for CREB1 and is essential for the activation of ApC/EBP required for the initiation of LTF. Combined, our data suggest that CAMAP is a retrograde signaling component that translocates from activated synapses to the nucleus during synapse-specific LTF.

Pentacene films on Si(1 0 0)-(2 × 1) surface at 300 K were investigated using near edge X-ray absorption fine structure (NEXAFS) at the carbon K-edge. NEXAFS spectra show that pentacene molecules are chemisorbed on the Si(1 0 0)-(2 × 1) surface for monolayer with flat-laying and predominantly physisorbed on the Si(1 0 0)-(2 × 1) surface for multilayer films with an upright molecular orientation. Absorption angle of pentacene molecules were measured through π ∗ transition. The angles between the double bond and the silicon surface were 35–55°, 65° and 76° at monolayer, 24 and 48 nm pentacene deposited on the Si(1 0 0) surface, respectively. We observed that the intermediate flat-laying phase is favored for monolayer coverage, while the films of molecules standing perpendicular to the Si(1 0 0) surface are favored for multilayer coverage.

Gastrin releasing peptide (GRP) is the first peptide isolated from porcine gastric and intestinal tissues and is homologous to the carboxyl terminus of bombesin (Bn) isolated from the skin of the frog Bombina bombina. It is a member of the Bn-like peptides, which are important in numerous biological and pathological processes. The Bn-like peptides show high sequence homology in their C-terminal regions, but they have different selectivity for their receptors. In particular, GRP selectively binds to the GRP receptor (GRPR). However, the molecular basis for this selectivity remains largely unknown. Here, we report the three-dimensional structure of GRP. Hopefully, it could be helpful in a better understanding of the binding selectivity between GRP and GRPR.

SummaryIn Aplysia, long-term synaptic plasticity is induced by serotonin (5-HT) or neural activity and requires gene expression. Here, we demonstrate that ApLLP, a novel nucleolus protein, is critically involved in both long-term facilitation (LTF) and behavioral sensitization. Membrane depolarization induced ApLLP expression, which activated ApC/EBP expression through a direct binding to CRE. LTF was produced by a single pulse of 5-HT 30 min after the membrane depolarization. This LTF was blocked when either ApLLP or ApC/EBP were blocked by specific antibodies. In contrast, ApLLP overexpression induced LTF in response to a single 5-HT treatment. Simultaneously, a siphon noxious stimulus (SNS) to intact Aplysia induced ApLLP and ApC/EBP expression, and single tail shock 30 min after SNS transformed short-term sensitization to long-term sensitization of siphon withdrawal reflex. These results suggest that ApLLP is an activity-dependent transcriptional activator that switches short-term facilitation to long-term facilitation.

The adsorption of 4-NO 2 (C 6 H 4 )COOH (4-nitro benzoic acid) on the in situ prepared OH/Si(1 0 0) 2 × 1 surface was investigated using X-ray photoelectron spectroscopy (XPS) and near-edge X-ray absorption fine structure (NEXAFS). XPS and NEXAFS results reveal that one 4-nitro benzoic acid (4-NBA) adsorbs on a OH group on the Si(1 0 0) through C–O–Si linkage induced by a dehydrating reaction between OH on the Si(1 0 0) and OH in the carboxyl group. Upon irradiating 4-NBA/OH/Si(1 0 0) surface by photons of 0.24 mJ/(s cm 2 ) (600 eV monochromatic beam), NO 2 moiety in the molecule was desorbed, whereas the phenyl ring and carboxyl group remained intact. The irradiation by the photon of 156.4 mJ/(s cm 2 ) (0th order beam) resulted in destruction of molecules on the surface and metathesis of NO 2 into NH 2 .

Background/Aims Mutations in hepatitis B virus (HBV) to lamivudine resistance that arise during prolonged treatment frequently cause amino acid substitutions in the YMDD motif of HBV DNA polymerase. Current methods of detecting such variants are time-consuming, labor intensive, and unsuitable for screening large numbers of samples. Here, we describe the development of a matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) genotyping assay suitable for detecting HBV variants in a sensitive and specific manner. Methods The assay is based on PCR amplification and mass measurement of oligonucleotides containing sites of mutation of the YMDD motif. Results The MALDI-TOF MS-based genotyping assay is sufficiently sensitive to detect as few as 100 copies of HBV genome per millilitre of serum, with superior specificity for determining mixtures of wild-type and variant viruses. When sera from 40 patients were analyzed, the MALDI-TOF MS-based assay correctly identified known viral variants and additional viral quasi-species not detected by previous methods, as well as their relative abundance. Conclusions The sensitivity, accuracy and amenability to high-throughput analysis makes the MALDI-TOF MS-based assay suitable for mass screening of HBV infected patients receiving lamivudine, and can help provide further understanding of disease progression and response to therapy.

The Diagnostic Interview for Genetic Studies (DIGS), developed in 1994 by the National Institute of Mental Health (NIMH), was translated into Korean and tested for reliability and diagnostic validity. Concurrent validity was tested using the Structured Clinical Interview for DSM-IV (SCID) and clinical diagnoses in 53 patients, most of whom had either schizophrenia or bipolar disorder. Inter-rater reliability was tested in 24 patients. Test-retest reliability was also tested in 17 patients. Overall and specific diagnostic validity for the Korean version of DIGS (DIGS-K) was excellent for most diagnoses. Inter-rater and test-retest reliability for overall and specific diagnoses also ranged from fair to excellent. For schizoaffective disorder, the test-retest reliability of DIGS-K was in a fair range, although the level was lower than that of other diagnoses. However, its diagnostic validity and inter-rater reliability was below fair range. In conclusion, DIGS-K appears to be a reliable interview for major psychiatric disorders.

The electrical properties of neurons are produced by the coordinated activity of ion channels. K+ channels play a key role in shaping action potentials and in determining neural firing patterns. Small conductance Ca2+-activated K+ (SK(Ca)) channels are involved in modulating the slow component of afterhyperpolarization (AHP). Here we examine whether rat type 2 SK(Ca) (rSK2) channels can affect the shape of the action potential and the neural firing pattern, by overexpressing rat SK2 channels in Aplysia neuron R15. Our results show that rSK2 overexpression decreased the intra-burst frequency and changed the regular bursting activity of neurons to an irregular bursting or beating pattern in R15. Furthermore, the overexpression of rSK2 channels increased AHP and reduced the duration of the action potential. Thus, our results suggest that ectopic SK(Ca) channels play an important role in regulating the firing pattern and the shape of the action potential.

The chemical and geometrical characteristics of pyrrole on Si(1 0 0)-2 × 1 were studied by using the photoemission spectroscopy (PES) and the near edge X-ray absorption fine structure (NEXAFS). A pyrrole layer was formed by exposing 20 L pyrrole molecules on the Si(1 0 0)-2 × 1 surface at 300 K. PES results confirmed that pyrrole reacts with the surface through the breakage of N H and C H groups, forming Si N bonds and Si C bonds between the Si surface and the pyrrole ring. NEXAFS studies showed that π-bonding state of pyrrole has angle dependence with respect to the surface normal. Thus, pyrrole was dissociatively adsorbed by [2 + 2] cycloaddition reaction with ∼16.5° tilt angle with respect to the surface normal direction at 300 K.

Recent studies have identified immunologic abnormalities in some schizophrenic subjects. This experiment replicates previous findings that serum soluble interleukin-2 receptors (SIL-2Rs) are elevated in schizophrenic patients, and is the first study to describe this phenomenon in non-Caucasian patients. Despite differences between Korean and Caucasian schizophrenic patients in absolute serum SIL-2R levels, both groups were significantly elevated when compared with their respective ethnic control groups (477 +/- 171 U/ml versus 354 +/- 172 U/ml and 763 +/- 347 U/ml versus 567 +/- 231 U/ml, respectively). Neither age, gender, medication status, nor duration of illness correlated with SIL-2R levels. These findings are further evidence that immune activation is present, regardless of ethnic origin, in some schizophrenic patients.

The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.

mal pregnant women (control), underwent overnight pulse oximetry and nasal pressure measurements (Embletta,Medcare) at mean gestation of 31 weeks.We applied The American College of Obstetricians and Gynecologists definitions for pre-eclampsia. Fasting blood samples were drawn for Tumor Necrosing Factor alpha (TNFa), Interleukin 6 (IL-6) and sensitive C Reactive Protein (CRP). Data from clinical evaluation of edema by analog scale (0 = no, 12 = severe edema), pregnancy outcomes, Epworth sleepiness scales (ESS) and Tiredness questionnaire (0 = no, 3 = severe) were collected. Results:No significant differences in age, body mass index (BMI) at sleep study, gestational age, bloodoxygen saturation (SpO2) or ESS were found between PE and controls. The nasal flow was significantly limited in PE women (Table 1), theywere significantly (p < 0.01)more tired than controls, mean (SD) 1.4 (0.5) vs. 0.1 (0.5), and had worse pregnancy outcomes (p < 0.05): 1-min Appgar score 7.9 (1.8) vs. 8.8 (0.4), relative birth weight (z-score) 1.9 (0.8) vs. +0.4 (1.2). The table shows the main findings: Conclusions: Pregnant women with pre-eclampsia showed significantly more nasal flow limitation during the night, higher fasting IL-6 and TNFa plasma levels, more edema and worse pregnancy outcomes than healthy pregnant women.