1. An application of the Rayleigh optics of the ultracentrifuge to molecular weight determinations by the Archibald method
- Author
-
Gene Conway and Joe L. Roberts
- Subjects
Optics and Photonics ,Polynomial ,Chemical Phenomena ,Chemistry ,Research ,Analytical chemistry ,Tangent ,Serum Albumin, Bovine ,Function (mathematics) ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Displacement (vector) ,Molecular Weight ,symbols.namesake ,Ribonucleases ,symbols ,Animals ,Cattle ,Ultracentrifuge ,Rayleigh scattering ,Concentration gradient ,Ultracentrifugation ,Serum Albumin - Abstract
For Archibald molecular weight calculations measurements are made along a single Rayleigh fringe. The displacement of the fringe measured as a function of the radial distance, x , is used to obtain the concentration gradient d c /d x by fitting a second-degree polynomial in x , extrapolating and differentiation. Good agreement with published values of the molecular weight was obtained with solutions of RNAase and of bovine serum albumin. Also described is a graphical method for obtaining d c /d x from a tangent to the fringe drawn upon a photographic enlargement.
- Published
- 1964
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