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The goal of fixation surgery for atlantoaxial instability is to achieve solid bony fusion. Achieving bony fusion as early as possible is beneficial for patients. Although placement of a transverse cross-link connector (XL) provides greater biomechanical strength, XLs have not been able to be placed when performing the Goel/Harms procedure. Recently, placing a XL on the screw head (on-the-head XL [OH-XL]) has become a viable option during the Goel/Harms procedure. However, there is little evidence demonstrating whether applying an OH-XL achieves early solid bony union. A matched-control study was conducted to investigate whether placing an OH-XLs in C1/2 fixation surgery provides earlier bone union or not. Eighteen patients who underwent atlantoaxial fusion with OH-XLs (X-group), and 17 age and sex-matched patients without OH-XLs (NX-group) were compared. Bony union was assessed using reconstructed sagittal and coronal computed tomography images. Six months after surgery, six patients in the X-group and one patient in the NX-group achieved bony union (p = .0338). One year after surgery, 14 patients in the X-group and 4 patients in the NX-group achieved bone union (p = .0010). Two years after surgery, 17 patients in the X-group and 9 patients in the NX-group achieved bony union (p = .0011). This is the first report of the clinical application of OH-XLs for the Goel/Harms procedure. OH-XLs yield earlier bony fusion; thus, placing OH-XLs is beneficial for atlantoaxial fixation surgery using lateral mass screws of the atlas.

The purpose of this study is to clarify the impact of low cost carriers (LCCs) on non-aeronautical revenues in 26 UK airports from 1999 to 2008. Increasingly non-aeronautical revenues have become an important source for airport revenue. Our literature review highlights that there is little consensus in empirical results concerning LCC impact on non-aeronautical revenues, some of these report positive impact while others show negative impact. We estimate a non-aeronautical revenue function which includes frequency share of LCC, Air Transport Movements (ATMs), number of passengers and population of hinterland of each observed airports as explanatory variables. In addition to this, we produce marginal revenue estimates for both the case of capacity constrained and capacity unconstrained circumstance in airports. As a result, in the case of a non-capacity constrained airport, on average, the marginal revenue of an additional LCC ATM is £147 while for non-LCC ATM it is £226. On the other hand, in the case of a capacity constrained airport, on average, substituting one non-LCC ATM with an LCC ATM reduces revenue by £79.

In Japan, the yardstick regulation was implemented as an incentive only for large private railway companies, in 1997. This study examined the effects of yardstick regulation on productivity of private railway companies by comparing TFP (Total Factor Productivity) of a large private railway group with that of a small private railway group in Kansai metropolitan area. We interpreted a railway's service to conduct the stratified 3T activities; Track, Train and Transport and estimated TFP for individual and total 3T activities. Estimation results showed that both railway groups had increased their TFP of total 3T activities contributed by productivity improvements of track and train activities since 1997. On the other hand, we could not find obvious evidences to show that the yardstick regulation for large railway companies had generated productivity improvements compared with small railway companies to which the traditional rate of return regulation is applied.

This study evaluated experimentally the influence of the swirling inflow condition upon the flow field in the cold leg piping, which comprises three 90 ° elbows, of the primary cooling system of Japan sodium-cooled fast reactor. Flow visualization experiment was conducted to reveal the velocity fields of the flow in the piping with swirling flow generator. When comparing to the developed turbulent inflow case, the flow separations in the intrados of the 1st elbow became smaller and the swirling flow downstream of the 2nd elbow became stronger, and there appeared separated regions downstream of the 2nd elbow which was not observed in developed turbulent inflow case. Since the flow separation can be considered as the source of the separated vortices which induce pressure fluctuations, these results imply the possibility that pressure fluctuations downstream of the 1st elbow are reduced and those of the 2nd elbow are enhanced by swirling flows as the inlet condition. The separated region is strongly reduced when a suitable swirling inflow enters the 3rd elbow.

In Japan, High Speed Rail (HSR) extended its network in 2011 and 2015, and Low Cost Carriers (LCC) entered into the domestic airline market in 2012. We compared the effects of the extension of the HSR network and the entry of LCCs on the airfares of the incumbent Full Service Carriers (FSC). We conducted Difference in Differences analysis with passenger level data to evaluate these market changes, and found that the effects of the HSR extension on FSC's airfares were consistently negative and larger in the short haul markets, while the effects of the entry of LCCs were inconsistent. HSR seems to be a stronger competitor than LCC to FSC and this finding could be affected by capacity, which is large for HSR while small for the LCCs.

Introduction Gait disturbance of Parkinsonian syndrome is characterized by dysrhythmic gait cycle including freezing, small steps and slowness. These symptoms are often not sufficiently responsive to dopaminergic therapy. Recently, we developed new rehabilitation system using non-invasive closed–circuit brain stimulation targeting on dysrhythmic gait dysfunction. Methods The objective of this study is to investigate whether the oscillatory closed-circuit brain stimulation mimicking the appropriate gait cycle can modulate the gait speed and cycle in patients with parkinsonian syndrome. Six patients with Parkinsonian syndrome participated in this study. Three were diagnosed as Parkinson’s disease, two as corticobasal syndrome and one as vascular parkinsonism. Before gait rehabilitation, we decided optimal brain stimulation frequency to each subject as 1/averaged gait cycle when they were best on condition. All participants performed gait rehabilitation for total ten days which contained 4 sessions (4 min. walk and 3 min. rest) per each day. During gait rehabilitation, slow oscillatory DC stimulation (current intensity 0–2 mA) was applied over ipsilateral cerebellum as symptom dominant side, which was triggered by their flat foot switch attached to the ipsilateral heels. Closed-circuit system enabled the implementation of the oscillatory brain stimulation with a fixed pre-set phase lag with respect to the intrinsic gait cycle. Ten m gait speed, cadence, laterality of gait cycle and frozen of gait questionnaire (FOGQ) were evaluated before and after gait rehabilitation. Results Gait speed and cadence were significantly increased after gait rehabilitation (speed: 98 ± 39 cm/s before and 116 ± 38 cm/s after, cadence: 114 ± 18 steps/min before and 124 ± 20 steps/min after). Laterality of gait cycle and FOGQ were significantly decreased after the rehabilitation (laterality: 0.07 ± 0.05 before and 0.0021 ± 0.004 after, FOGQ: 10 ± 7 before and 7 ± 5 after). Conclusion Closed-circuit brain stimulation during walking is useful method to parkinsonian gait disturbance possibly by normalizing dysrhythmic and lateralized gait control system in patients with Parkinsonian syndrome. This new system may provide the personalized rehabilitation in patients with Parkinson related disorders.

Resveratrol, a natural polyphenol abundantly found in grape skins and red wine, possesses various beneficial properties for human health. In the present study, we investigated the mechanism underlying the effects of prostaglandin F2α (PGF2α) on osteoprotegerin (OPG) synthesis and of resveratrol on the OPG synthesis in osteoblast-like MC3T3-E1 cells. PGF2α stimulated both the release of the OPG protein and the expression of OPG mRNA. Treatment with PD98059, SB203580 and SP600125, specific inhibitors of MEK1/2, p38 mitogen-activated protein (MAP) kinase and stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) all suppressed the OPG release induced by PGF2α. Resveratrol also significantly reduced the PGF2α-stimulated OPG release and the mRNA levels of OPG. Similarly, treatment with SRT1720, an activator of SIRT1, also suppressed the PGF2α-stimulated OPG release. Resveratrol and SRT1720 both attenuated the phosphorylation of p44/p42 MAP kinase, MEK1/2, Raf-1, p38 MAP kinase and SAPK/JNK induced by PGF2α. These findings strongly suggest that resveratrol suppresses PGF2α-stimulated OPG synthesis by inhibiting the MAP kinase pathways in osteoblasts, and that the effect is mediated via SIRT1 activation.

We previously reported that interleukin-1 (IL-1), a potent bone resorptive cytokine, stimulates the synthesis of interleukin-6 (IL-6) via activation of p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells, and that AMP-activated protein kinase (AMPK) negatively regulates the IL-1-induced IL-6 synthesis through the inhibitor of κB (IκB)/nuclear factor-κB (NF-κB) pathway. On the other hand, it is recognized that catechin possesses a beneficial property for bone metabolism. Among them, (-)-epigallocatechin gallate (EGCG) is an abundant and major bioactive component. In the present study, we investigated the effect of EGCG on the IL-1 stimulated IL-6 synthesis in osteoblast-like MC3T3-E1 cells. EGCG significantly enhanced the IL-1-stimulated IL-6 synthesis in a dose-dependent manner in the range between 50 and 100 μM. EGCG increased the mRNA levels of IL-6 stimulated by IL-1. IL-1-induced phosphorylation of IκB and NF-κB were suppressed by EGCG. On the other hand, EGCG failed to affect the IL-1-induced phosphorylation of p44/p42 MAP kinase, p38 MAP kinase and AMPK. These results strongly suggest that EGCG enhances IL-1-stimulated IL-6 synthesis through inhibiting the AMPK-IκB/NF-κB pathway at the point between AMPK and IκB/NF-κB in osteoblasts.

Stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK)(1) which belongs to the MAP kinase superfamily regulates many cellular events. We previously reported that interleukin 1 (IL-1) stimulates the synthesis of interleukin 6 (IL-6) through activation of ERK and p38 MAP kinase in osteoblast-like MC3T3-E1 cells, and that AMP-activated protein kinase (AMPK) negatively regulates the IL-1-induced IL-6 synthesis through IκB/NF-κB pathway. In the present study, we investigated the role of SAPK/JNK in the IL-1-stimulated IL-6 synthesis in these cells. IL-1 induced the phosphorylation of SAPK/JNK. SP600125, an inhibitor of SAPK/JNK, increased the release and the mRNA expression levels of IL-6 induced by IL-1. IL-1-stimulated IL-6 release was significantly up-regulated in SAPK/JNK-knocked down cells. SP600125 remarkably suppressed the IL-1-induced phosphorylation of both IκB and NF-κB, whereas SP600125 failed to affect the IL-1-induced phosphorylation of AMPK, STAT3 or Src. Compound C, an AMPK inhibitor, attenuated the IL-1-induced phosphorylation of SAPK/JNK. SP600125 enhanced IL-1-stimulated IL-6 release also in normal human osteoblasts. These results strongly suggest that SAPK/JNK negatively regulates IL-1-stimulated IL-6 synthesis and acts at the point between AMPK and IκB/NF-κB in osteoblasts.

Evidence is accumulating that Rho-associated kinase (Rho-kinase) plays important roles not only in vascular smooth muscle cell contraction, but also in a variety of cellular functions, including bone metabolism. In the present study, we investigated the involvement of Rho-kinase in the osteocalcin synthesis induced by triiodothyronine (T3) in osteoblast-like MC3T3-E1 cells. T3 time-dependently induced phosphorylation of myosin phosphatase targeting subunit (MYPT-1), a substrate of Rho-kinase. Y27632, a specific inhibitor of Rho-kinase, attenuated the MYPT-1 phosphorylation induced by T3. T3-stimulated osteocalcin release was significantly enhanced by Y27632. Fasudil, another Rho-kinase inhibitor, amplified the osteocalcin release induced by T3. T3-stimulated osteocalcin release was significantly augmented in Rho-knockdown cells with Rho A-siRNA. Y27632 and fasudil also increased the mRNA expression level of osteocalcin induced by T3. These results strongly suggest that T3 stimulates the activation of Rho-kinase in osteoblasts, which functions as a negative regulator of T3-stimulated osteocalcin synthesis.

In 2003, Japan Airlines and Japan Air System merged to become an equal rival to All Nippon Airways, which had significant market power in the domestic air transportation market in Japan. This paper examines the merger effects on the competition structure of the air transportation market using conduct parameter and theoretical price approaches. We test to see if the merger changed the market structure because there had been a leader–follower relationship among three carriers before the merger, and we had an equal competitor relationship between two carriers after the merger.

Our previous study demonstrated that tumor necrosis factor-alpha (TNF-alpha) stimulates the synthesis of interleukin-6 (IL-6), a potent bone resorptive agent, via p44/p42 mitogen-activated protein (MAP) kinase and phosphatidylinositol 3-kinase/Akt in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether p70 S6 kinase is involved in TNF-alpha-stimulated IL-6 synthesis in MC3T3-E1 cells. TNF-alpha time dependently induced the phosphorylation of p70 S6 kinase. Rapamycin, an inhibitor of p70 S6 kinase, which attenuated the phosphorylation of p70 S6 kinase induced by TNF-alpha, significantly amplified the TNF-alpha-stimulated IL-6 synthesis. TNF-alpha-induced phosphorylations of both p44/p42 MAP kinase and Akt were markedly enhanced by rapamycin. The amplification by rapamycin of TNF-alpha-induced IL-6 synthesis was reduced by PD98059, a specific inhibitor of MEK1/2, or Akt inhibitor. Rapamycin enhanced the IL-6 synthesis and the phosphorylation of Akt induced by TNF-alpha also in human osteoblasts. Taken together, these results strongly suggest that p70 S6 kinase limits the TNF-alpha-stimulated IL-6 synthesis at a point upstream from p44/p42 MAP kinase and Akt in osteoblast-like cells.

Although exercise is believed to reduce the risk of rupture of the myotendinous junction, exercise-induced structural changes in this region have not been studied. We examined exercise-induced ultrastructural changes in the myotendinous junction of the lower legs in rats.Ten adult male LETO rats were used. Five rats were randomly placed in the Exercise group; the remaining five were used as controls and placed in the non-Exercise group. Running exercise was performed every day for 4 weeks. The tibialis anterior and gastrocnemius muscles were then removed from both legs from each animal in the two groups. The specimens were subsequently examined by transmission electron microscopy (TEM). Numerous finger-like processes were observed at the myotendinous junction. The changes in frequency of branching of the finger-like process (the number of times one finger-like process branched) and the direction of the processes (the angle of the major axis of a finger-like process to the longitudinal direction of the muscle fiber) were studied. To evaluate the two indicators above, each 10 fingerlike process was randomly and separately selected from the tibialis anterior and gastrocnemius muscles of rats, providing 50 finger-like processes of both muscles for evaluation per group.In terms of the frequency of branching of the fingerlike processes, the mean values obtained in the non-Exercise group were 0.04 and 0.18 times, respectively, in the tibialis anterior and gastrocnemius muscles and were 0.38 and 1.16 times, respectively, in these two muscles in the Exercise group. Regarding the direction of the finger-like processes, the values were 4.1 degrees and 3.6 degrees, respectively in the non-Exercise group and 10.4 degrees and 14.5 degrees , respectively in the Exercise group. The differences between the two animal groups were significant.Morphological changes in the myotendinous junction occurred as an adaptation to tension increased by exercise.

A cross-shaped tube bundle is proposed for the lower plenum structure in next-generation LWRs. The vibration response of the cross-shaped tube bundle in cross-flow has been measured in water tunnel tests. First, a small-scale test was conducted. Tests were conducted with 3×3 flexible tubes as well as a single flexible tube in a rigid tube bundle. The flexible tubes could vibrate in the lift, drag, and torsional directions. The effect of the arrangement of the tube bundle and the natural frequency ratio of translational and torsional vibrations were considered. Second, a large-scale test was conducted for only one case to check the effect of Reynolds number, in which the Reynolds number was 10 times larger than that of the small-scale test. In all the cases, large-amplitude vibration appeared when the flow velocity became larger than a critical value, and self-excited vibration was found to occur. The nondimensional critical gap velocity of the large-scale test agreed well with that of the small-scale test, which suggested that the effect of Reynolds number was not so large. A design guideline to prevent self-excited vibration is proposed for the cross-shaped tube bundle.

A cross-shaped tube bundle with small ratio of the gap width to tube diameter may be designed for the lower plenum structure in a next-generation BWR, though the characteristics of flow-induced vibration of this type of tube bundle remain virtually unknown. In this study, fluid dynamic forces acting on a cross-shaped tube bundle in a normal arrangement with small ratio of the gap width to tube diameter subjected to cross-flow were measured by water tunnel tests with two types of scale model. One was a small-scale model to measure local fluid dynamic forces and their correlation length in the lift and drag directions. The other was a large-scale model to investigate the effect of the Reynolds number on fluid dynamic forces in the lift, drag and torsional directions. Free oscillation tests with another small-scale model were also conducted to measure vibration amplitude by random excitation force. The following results were obtained. The measured fluid dynamic drag, lift and torque acting on the cross-shaped tube bundle in cross-flow were successfully compiled into the correlation equations, which include the nondimensional power spectral density (NPSD) of the local fluid dynamic force and the spanwise correlation length. The Reynolds effects on the NPSD can be considered negligible at least in the range of Reynolds number 6 . No remarkable spectral peaks of vortex shedding appeared in the NPSD, and the ratios of the correlation lengths to the tube diameter for the cross-shaped tube bundle were much shorter than that of the random excitation force acting on circular tube bundles. It is considered that this was because the ratio of gap width to tube diameter was small for the tested arrangement.

A new 2,5-bis(ferrocenyl)ruthenacyclopentatriene complex was synthesized by the metallacycle formation reaction of RuBrCp(cod) (Cp=η 5 -C 5 H 5 ) and ethynylferrocene. Cyclic voltammetry of the complex showed a reversible one-electron reduction due to the ruthenacycle and a two-step one-electron oxidation of the ferrocenyl moieties. Separation of the redox potentials of the ferrocenyl moieties was 0.24 V, and the electronic spectrum of one-electron oxidized species exhibited an intervalence-transfer band at 1180 nm. These results indicate the existence of significant electronic interactions between two ferrocenyl moieties through the ruthenacyclopentatriene ring.

In the present study, we developed a device for the non-destructive detection of abnormal eggs (mainly eggs with cracked shells, eggs with leaking cracks, unclean eggs, and eggs with blood spots). This device incorporates color and monochrome CCD cameras. Determination of eggs with a cracked shell is done by processing the transmission image of the egg acquired using the color CCD camera. To determine eggs with blood spots, eggs are irradiated with light of the wavelength band that absorbs blood and the permeating light is captured with the monochrome CCD camera, after which the image data are processed. The results suggest that all types of abnormal eggs can be detected with a high precision of 95.0%~98.5%.

Microgram or nanogram quantities of heavy metals adsorbed on suspended solids are selectively floated to the solution surface by bubbling for 15 s. Prior to the flotation, negatively charged suspended solids are rendered hydrophobic and coagulated by stirring with a cationic surfactant and sodium chloride. Metal ions, humic acid complexes and colloidal hydrated metal oxides are not floated at all. After the separation of the suspended solids, the trace heavy metals are desorbed by ultrasound in 4 M nitric acid and determined by electrothermal atomic absorption spectrometry. This rapid and convenient flotation technique is successfully applied to the analysis of river, pond and waste waters.

The disintegration behaviour of liposomes in polyoxyethylene lauryl ether (PLE) and sodium cholate solutions was studied by the turbidity disappearance method. In maximally solubilized systems of liposomes, the molar ratios (phosphatidylcholine/surfactant) were 0.43 and 1.8 for PLE and sodium cholate, respectively. The disintegration process of either unilamellar or multilamellar liposomes followed first-order kinetics. Based on a physical model in which liposomes heterogeneous in size were assumed to disintegrate from the outermost shell one by one, a mathematical expression of the turbidity disappearance rate was introduced and applied to explain the data thus obtained. Model calculations suggested that the number of disintegrated shells would not be so large, even if up to 50% reduction of the initial turbidity was observed. From the dependence of the pseudo-first-order rate constant ( k obs ) on the surfactant concentration for unilamellar liposomes, it was assumed in general that k obs consists of the contributions of the monomer and micellar fractions: for PLE, both fractions shared in the disintegration, but only the micellar fraction with sodium cholate. Furthermore, in the latter case, k obs depended on the initial liposome concentration. These results are likely to be consistent with the proposed modes of surfactant action classified as type A and type B (Helenius, A. and Simons, K. (1975) Biochim. Biophys. Acta 415, 29–79).