1. Evaluation of Measles Igm Antibody Detection Assays During the 2018-2019 Outbreak in New York State
- Author
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Kyle J. Carson, Heidi R. Tucker, Kelly Howard, Margaux Hales, Patrick W. Bryant, Kirsten St. George, Karen E. Kulas, and William T. Lee
- Subjects
Microbiology (medical) ,History ,Polymers and Plastics ,New York ,Enzyme-Linked Immunosorbent Assay ,General Medicine ,Antibodies, Viral ,Sensitivity and Specificity ,Industrial and Manufacturing Engineering ,Disease Outbreaks ,Infectious Diseases ,Immunoglobulin M ,Humans ,Serologic Tests ,Business and International Management ,Measles - Abstract
Although measles was eliminated in the United States in 2000, a severe outbreak occurred between October 2018 and September 2019. New York was especially hard hit. Serology played an integral role in determining immune status (IgG) and identifying, along with molecular analyses, acute measles infections (IgM). Although an indirect immunofluorescence assay (IFA) was historically used by the New York State Department of Health for measles IgM detection, a higher throughput assay was needed to address the increased specimen numbers. Four commercial enzyme-linked immunosorbent assays (ELISAs) were evaluated for sensitivity and specificity in detecting measles IgM. Two ELISA formats were compared, indirect ELISA and IgM antibody capture. Both formats had comparable specificity as determined by cross-reactivity to non-measles specimens. Overall, the sensitivity of the capture ELISAs was greater than the indirect ELISAs and comparable to the indirect immunofluorescence assay with benefits regarding capacity, cost, and turnaround time.
- Published
- 2022
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