1. High-performance liquid chromatographic analysis of sulfinpyrazone and its metabolites in biological fluids
- Author
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Thorir D. Bjornsson, Katherine M. Wolfram, Philip A. Routledge, and David G. Shand
- Subjects
Male ,chemistry.chemical_classification ,Chromatography ,Base (chemistry) ,Metabolite ,Extraction (chemistry) ,General Chemistry ,Sulfinpyrazone ,Sulfone ,chemistry.chemical_compound ,chemistry ,Sodium hydroxide ,Butanes ,medicine ,Humans ,Sample preparation ,Sulfones ,Ethylene Dichlorides ,Quantitative analysis (chemistry) ,Chromatography, High Pressure Liquid ,medicine.drug - Abstract
A rapid, senstivie, and specific high-performance liquid chromatographic method is described for the quantitative analysis of sulfinpyrazone and its sulfone and p-hydroxy metabolites in plasma and urine. The method uses two different procedures for sample preparation: (1) a rapid and convenient procedure using a single extraction with 1-chlorobutane and subsequent back-extraction into sodium hydroxide solution for the analysis of sulfinpyrazone and its sulfone metabolite, and (2) a more time consuming procedure using triple extraction with ethylene dichloride, a buffer wash, and back extraction into the base for the additional analysis of the p-hydroxy metabolite. The lower limit of sensitivity for fulfinpyrazone is 50 ng/ml. Concentrations of sulfinpyrazone between 0.05 and 0.1 and 50 micrograms/ml were measured with an average coefficient of variation of 3.9%, ranging from 1.5 to 6.1%.
- Published
- 1980
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