Search

International audience; We propose in the present work a numerical solution employed to treat the coupled Monte Carlo method and Poisson equations. This technique is capable of capturing some important features of semiconductor devices.Numerical results are presented for one-dimensional Hg0.8Cd0.2Te n nn structure, the presence of velocity overshoot has been observed and it is recognized that the fluctuation of velocity and energy term plays an important role in the simulation of semiconductor devices.

Introduction Highly specific targeting of human malignant cells with the purpose of recognition and delivery of specific drugs into them is a very promising but not well developed complex of diagnostic and therapeutic strategies. It is a major focus of current molecular cancer research, Nanomedicine, Biotechnology and Synthetic Biology. Material and methods THP-1 and primary human AML cells were used in our work. We constructed a complex, where 5 nm AuNPs carried a 27 kDa single chain antibody against T cell immunoglobulin and mucin domain 3 (Tim-3) in the ratio – 1 AuNP: 1 antibody molecule. The rest of the gold surface was covered with rapamycin immobilised in the form of glutathione-SH ester. Transmission electron microscopy and synchrotron radiation circular dichroism spectroscopy were used to characterise nanomaterials. Western blot analysis, FACS, in cell Western and ELISA were used to conduct experiments. Results and discussions In this study we demonstrated for the first time a new approach for highly specific targeting of human AML cells by functionalised gold nanoconjugates. The immune receptor Tim-3 is highly expressed in human AML cells. It is one of the key components of Tim-3/galectin-9 secretory pathway which is crucial for survival of malignant cells since it determines their ability to escape host immune surveillance.1 Thus, Tim-3 can be used as a target for specific recognition of AML cells. Rapamycin inhibits activity of mammalian target of rapamycin (mTOR), a master regulator of translational pathways in AML cells. Inhibiting the mTOR leads to a rapid killing of AML cells. Using these nanoconjugates we managed to successfully deliver rapamycin into the AML cells reaching attenuation of the mTOR activity. Concentration of rapamycin required to reach such an effect is at least 50 times lower compared to the one of free rapamycin required to achieve similar effect. Conclusion We therefore concluded that our technology is of potential use for highly specific targeting of AML cells for the purpose of diagnosis and possibly therapy.2 The nanoconjugates can be used to specifically identify malignant blood cells thus allowing rapid AML diagnosis. References Goncalves Silva I, Yasinska IM, et al. E Bio Medicine 2017;22:44–57. Yasinska IM, et al. Nanoscale 2018 (in press).

In order to give an overview of the changes that have occurred in the coastal landscape of Italy, we have selected four examples for discussion. Two lie along the Adriatic Sea (the delta of the Tagliamento River and a section of the Ravenna coastline); the other two lie along the Tyrrhenian Sea (the delta of Tiber River and a section of the Tuscany coastline). The present day landscape evidences many changes. Whereas, in the past, there were extensive ridges of sand dunes and marshy areas, there are now new settlements. The coincidence of the natural processes of shore erosion and tourist activities along these sandy shores has led to the construction of breakwaters and other defensive structures, resulting in almost complete artificial stabilization of these shorelines.