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Aberrant angiogenesis is a hallmark of cancer and is critically associated with tumor progression. Perivascular cells are essential components of blood vessels, and the role of tumor perivascular cell-derived extracellular vesicles (TPC-EVs) in angiogenesis remains elusive. In the present study, using genetic mouse models and pharmacological inhibitors, we found that ablation of perivascular cells inhibited angiogenesis in allografted colorectal cancer tumors. Further studies demonstrated that TPC-EVs promoted the proliferation, migration, invasion, viability, and tube formation of HUVECs. They also facilitated vessel spouting in rat aortic rings and induced neovascularization in chick chorioallantoic membranes (CAMs). Silencing of Gas6 or blockade of the Axl pathway suppressed TPC-EV-induced angiogenesis in vitro and ex vivo. Moreover, inhibition of the Gas6/Axl signaling pathway impaired TPC-EV-mediated angiogenesis in vivo. Our findings present a deeper insight into the biological functions of TPCs and TPC-EVs in tumor angiogenesis and demonstrate that TPC-EV-derived Gas6 could be an attractive and innovative regulator of tumor angiogenesis.

Fibroblast-like synoviocytes (FLSs) are essential for joint destruction in rheumatoid arthritis (RA). 6-Shogaol, a phenolic extract isolated from ginger, has been found to have potential benefits in the treatment of diverse inflammatory and immune disorders. However, the role of 6-shogaol in RA has yet to be explored.To reveal the effect of 6-shogaol on RA FLSs and MH7A cells and to investigate the molecular mechanism of 6-shogao in RA.We performed MTT, EdU, cell apoptosis, cell migration and invasion, RT-qPCR, western blot analysis, and immunofluorescence to elucidate the effect of 6-shogaol on the proliferation, apoptosis, and migration of RA FLSs and MH7A cells and revealed its modulation of the PI3K/AKT/NF-κB pathway. The in vivo therapeutic effect of 6-shogaol was verified in mice with collagen-induced arthritis (CIA).6-Shogaol suppressed proliferation, migration, and invasion, and induced apoptosis in RA FLSs and MH7A cells. 6-Shogaol also reduced the production of TNF-α, IL-1β, IL-6, IL-8, MMP-2, and MMP-9. Molecular analysis revealed that 6-shogaol inhibited the PI3K/AKT/NF-κB pathway by activating PPAR-γ. Treatment with 6-shogaol ameliorated joint destruction of mice with CIA.This study revealed that 6-shogaol inhibited proliferation, migration, invasion, cytokine, and MMPs production, and induced apoptosis in RA FLSs via the PI3K/AKT/NF-κB pathway, providing a new natural potential drug for future RA treatments.

Dynamic simulation experiments were conducted on calcium carbonate fouling formation in shell and tube heat exchangers by using a self-designed online evaluation experimental platform of the electromagnetic anti-fouling effect to obtain the experimental data of conductivity, pH, dissolved oxygen and fouling resistance with the electromagnetic anti-fouling treatment (EAT). And the Elman neural network (Elman NN) was optimized using the genetic algorithm (GA) to derive the GA–Elman neural network (GA–Elman NN). On the basis of GA–Elman NN, a fouling resistance prediction model was established with conductivity, pH, and dissolved oxygen as the input variables and fouling resistance as the output variable. Prediction results indicated that GA–Elman NN improved the weight and threshold, overcame the drawback of falling into the local minimum, and strengthened the capability of finding the optimal solution, thereby improving the prediction accuracy significantly. Moreover, the GA–Elman NN prediction model presented enhanced generalization capability. The mean absolute percent error was 6.07%, and the total error was 8.78% with the experimental system uncertainty. These values indicate that the GA-Elman NN prediction model possesses the high prediction accuracy and is rational and feasible in predicting fouling resistance.

© 2019 Elsevier Ltd A new hybrid system was developed in this study for the treatment of drinking water consisting of pre-coagulation using polyaluminium chloride (PACl) and membrane filtration (MF) with sponge cubes acting as biomass carriers (P-SMF). When compared to a conventional MF (CMF) and a MF after coagulation by utilizing PACl (P-MF), better removal of nutrients, UV254 and dissolved organic carbon (DOC) (>65%) was obtained from the P-SMF. The accumulation of biopolymers (including polysaccharides and proteins), humic substances, hydrophilic organics, and other small molecular weight (MW) organic matter in the CMF led to the most severe membrane fouling coupled with the highest pore blocking and cake resistance. Pre-coagulation was ineffective in eliminating small MW and hydrophilic organic matter. Conversely, the larger MW organics (i.e. biopolymers and humic substances), small MW organics and hydrophilic organic compounds could be removed in significantly larger quantities in the P-SMF by PACl coagulation. This was achieved via adsorption and the biodegradation by attached biomass on these sponges and by the suspended sludge. Further analyses of the microbial community indicated that the combined addition of PACl and sponges generated a high enrichment of Zoolgloea, Amaricoccus and Reyranella leading to the reduction of biopolymers, and Flexibacter and Sphingobium were linked to the degradation of humic substances. Moreover, some members of Alphaproteobacteria in the P-SMF may be responsible for the removal of low MW organics. These results suggest that the pre-coagulation process coupled with adding sponge in the MF system is a promising technology for mitigating membrane fouling.

The aggressive phenotype displayed by fibroblast-like synoviocytes (FLSs) contributes to cartilage and bone destruction in rheumatoid arthritis (RA). Betulinic acid has been demonstrated to have a positive therapeutic effect on tumor, inflammation and immune disorder, however, the effects of betulinic acid on RA FLSs have not been verified. Therefore, in the present study, we observed the effect of betulinic acid on the migration and invasion of RA FLSs and explored its underlying signal mechanisms. Our results showed that betulinic acid treatment suppressed the migration, invasion and reorganization of the actin cytoskeleton of RA FLSs. In addition, we found that the mRNA expression of IL-1β, IL-6, IL-8 and IL-17A were markedly down-regulated by treatment with betulinic acid in TNF-α-induced RA FLSs. To gain insight into the molecular mechanisms, we evaluated the effect of betulinic acid on NF-κB activation in RA FLSs. The results indicated that betulinic acid treatment reduced the TNF-α-induced activation of NF-κB signal pathway and the NF-κB nuclear accumulation. We also observed that treatment with betulinic acid attenuated synovial inflammation and joint destruction in mice with CIA. Taken together, these results suggest that betulinic acid inhibits the migration and invasion of RA FLSs by blocking NF-κB signal pathway activation.

Angiogenesis is a hallmark of cancer and is required for tumor growth and progression. Antiangiogenic therapy has been revolutionarily developing and was approved for the treatment of various types of cancer for nearly two decades, among which bevacizumab and sorafenib continue to be the two most frequently used antiangiogenic drugs. Although antiangiogenic therapy has brought substantial survival benefits to many cancer patients, resistance to antiangiogenic drugs frequently occurs during clinical treatment, leading to poor outcomes and treatment failure. Cumulative evidence has demonstrated that the intricate interplay among tumor cells, bone marrow-derived cells, and local stromal cells critically allows for tumor escape from antiangiogenic therapy. Currently, drug resistance has become the main challenge that hinders the therapeutic efficacies of antiangiogenic therapy. In this review, we describe and summarize the cellular and molecular mechanisms conferring tumor drug resistance to antiangiogenic therapy, which was predominantly associated with redundancy in angiogenic signaling molecules (e.g., VEGFs, GM-CSF, G-CSF, and IL17), alterations in biological processes of tumor cells (e.g., tumor invasiveness and metastasis, stemness, autophagy, metabolic reprogramming, vessel co-option, and vasculogenic mimicry), increased recruitment of bone marrow-derived cells (e.g., myeloid-derived suppressive cells, tumor-associated macrophages, and tumor-associated neutrophils), and changes in the biological functions and features of local stromal cells (e.g., pericytes, cancer-associated fibroblasts, and endothelial cells). We also review potential biomarkers to predict the response to antiangiogenic therapy in cancer patients, which mainly consist of imaging biomarkers, cellular and extracellular proteins, a certain type of bone marrow-derived cells, local stromal cell content (e.g., pericyte coverage) as well as serum or plasma biomarkers (e.g., non-coding RNAs). Finally, we highlight the recent advances in combination strategies with the aim of enhancing the response to antiangiogenic therapy in cancer patients and mouse models. This review introduces a comprehensive understanding of the mechanisms and biomarkers associated with the evasion of antiangiogenic therapy in cancer, providing an outlook for developing more effective approaches to promote the therapeutic efficacy of antiangiogenic therapy.

Depression is a prevalent emotion disorder which is thought to be due to neuronal structural alterations and/or functional impairment within specific brain regions. Several studies have shown that microRNAs are involved in the pathogenesis of depression. As a Chinese herbal formula, Xiaoyaosan (XYS) could have antidepressive effects, although the mechanisms associated with microRNAs are poorly understood.In this study, we investigated whether inhibition of the miR-200a/b-3p/NR3C1 pathway in the prefrontal cortex is involved in the anti-neuronal apoptosis and anti-stress effects of XYS and then further delineated the underlying mechanism.To evaluate the efficacy of XYS in relieving stress behaviors and altering the expression of miRNAs involved in the regulation of these behaviors in vivo, a chronic unpredictable mild stress (CUMS) rodent model and RNA-seq were performed. Primary cortical neurons were used to evaluate the molecular function of miR-200a/b-3p and detect the in vitro neuroprotective function of paeoniflorin, which is one of the main components of XYS. To investigate the function of miR-200a/b-3p in stress behaviors, stereotactic microinjection of AAV2/9-Syn-miR-200a/b-3p was performed to deliver the treatment to the rat mPFC.XYS reduced the anxiety and depression-like behaviors associated with chronic stress and reduced the expression of miR-200a/b-3p and neuronal apoptosis in the prefrontal cortex (PFC). The overexpression of miR-200a/b-3p in primary cortical neurons reduced the expression of the target gene NR3C1, increased the protein expression of cleaved caspase-3 and Bax, and decreased the anti-apoptotic protein Bcl-2. One of the active ingredients of XYS, paeoniflorin, can inhibit miR-200a/b-3p-mediated apoptosis of primary neurons and abnormal expression of apoptosis-related proteins. After overexpressing miR-200a/b-3p in vivo (vmPFC), the rats eventually showed significant anxiety-like behaviors similar to those caused by chronic stress.Our findings indicate that XYS can inhibit the CUMS-induced expression of miR-200a/b-3p, regulate miR-200a/b-3p/NR3C1 signaling in the PFC caused by chronic stress, and reduce neuronal apoptosis and stress-related behaviors.

Anaerobic membrane bioreactors (AnMBRs), the combination of anaerobic digestion and membrane technology, have gained increasing popularity due to their remarkable advantages over aerobic membrane bioreactors, such as biogas production and potential energy use. However, membrane fouling remains a challenging issue that deteriorates the performance of membrane and shortens its lifespan. Pretreatment of feed wastewater by adding fouling reduction enhancers, such as adsorbents and flocculants, into anaerobic membrane bioreactor can effectively mitigate membrane fouling by altering the feed properties. Activated carbon, such as powdered activated carbon (PAC) and granular activated carbon (GAC), has been widely applied as an adsorbent to aerobic and anaerobic membrane bioreactors for membrane fouling control. Organic enhancers such as biochar and waste yeast, and inorganic enhancers like polyaluminum chloride and zeolite have also been applied to AnMBRs promoting flocculation and coagulation. Thus, this review discusses the impacts of different fouling reduction enhancers under anaerobic conditions as well as AnMBR system. In addition, the mechanisms of the enhancers mitigating the membrane fouling are also summarized for better understanding of the effects of the enhancers in AnMBRs.

T-cell receptor (TCR)-transduced T (TCR-T) cell therapy has shown promising efficacy in the clinical treatment of malignant cancers. However, the populations covered by reported TCRs are still limited. Tumor infiltrating lymphocytes (TILs) are natural reservoirs of tumor-reactive T cells and TCRs. Approaches are required for the fast and cost-effective identification of tumor-reactive TCRs from TILs. The widely employed TCR identification approaches by the clonal expansion of TILs involve a TCR singularization process for the direct pairing of TCR Vα and the Vβ chain. However, the clonal expansion of T cells is well known to require extensive time and effort due to the involvement of T cell cultures. Several single-cell multiplexing PCR methods followed by Sanger sequencing have been developed, representing a cost-effective and fast approach for single-cell TCR identification. In this study, an RNA-based preamplification step was included in the single-cell TCR sequencing, which can reduce the multiplexing PCR amplification to one round. Moreover, the cDNA product of RNA preamplification is derived from the whole genome mRNA, instead of TCR mRNA only by multiplexing primers-based DNA preamplification, which is valuable for many other analyses (e.g., phenotypic analysis) of the tumor-reactive T cells that can be correlated with the identified TCRs. The feasibility for both single α chain and dual α chain TILs of this approach highlights its potential value as a rapid and cost-effective sequencing strategy for the development of TCR-T therapies for solid cancers.

This study investigated the possibility of using a novel type of surface conductive hollow fiber membrane during the anaerobic membrane bioreactor process (AnMBR). In particular, the membrane fouling control mechanisms of Surface electric field AnMBR (S-AnEMBR) were evaluated through comparing with the operation of Conventional AnMBR (AnMBR) and Electric field AnMBR (AnEMBR). Results showed that S-AnEMBR had longer running time, stronger anti-fouling capability, and more stable total organic carbon (TOC) removal efficiency. And S-AnEMBR could extend the running time of the rapid fouling phase (Stage 2). Electrochemical analysis and microbial community analysis showed that enhanced electron transfer in S-AnEMBR accelerated the decomposition of organic matter and further improved acidification efficiency from 83.75% to 234.78%, indicating the methane concentration ranged between 24.56 mmol/L and 36.99 mmol/L. Cake layer compression was reduced because significant changes were observed in increased particle size and more negative Zeta potential. In addition, basophilic methanogens (48%) were enriched at cathode, which improved electrochemical activity of carbon electrode biocathode and promoted anaerobic digestion. Therefore, it was found that S-AnEMBR was more conducive to controlling the properties and structure of the gel layer, and further achieving the resistance towards protein and polysaccharide adhesion in membrane. This work demonstrated that the surface conductive hollow fiber membrane possessed good anti-fouling ability in AnMBR, and provided an alternative membrane material for AnMBR application.

To tackle membrane fouling and limited removals of pollutants (nutrients and emerging pollutants) that hinder the wide applications of membrane bioreactor (MBR), attached growth MBR (AGMBR) combining MBR and attached growth process has been developed. This review comprehensively presents the up-to-date developments of media used in both aerobic and anaerobic AGMBRs for treating wastewaters containing conventional and emerging pollutants. It also elaborates the properties of different media, characteristics of attached biomass, and their contributions to AGMBR performance. Conventional media, such as biological activated carbon and polymeric carriers, induce formation of aerobic, anoxic and/or anaerobic microenvironment, increase specific surface area or porous space for biomass retention, improve microbial activities, and enrich diverse microorganisms, thereby enhancing pollutants removal. Meanwhile, new media (i.e. biochar, bioaugmented carriers with selected strain/mixed cultures) do not only eliminate conventional pollutants (i.e. high concentration of nitrogen, etc.), but also effectively remove emerging pollutants (i.e. micropollutants, nonylphenol, adsorbable organic halogens, etc.) by forming thick and dense biofilm, creating anoxic/anaerobic microenvironments inside the media, enriching special functional microorganisms and increasing activity of microorganisms. Additionally, media can improve sludge characteristics (i.e. less extracellular polymeric substances and soluble microbial products, larger floc size, better sludge settleability, etc.), alleviating membrane fouling. Future studies need to focus on the development and applications of more new functional media in removing wider spectrum of emerging pollutants and enhancing biogas generation, as well as scale-up of lab-scale AGMBRs to pilot or full-scale AGMBRs.

Diabetic nephropathy (DN) contributes to end-stage renal disease and kidney dysfunction with a proverbial feature of podocyte injury. Inflammation and insulin resistance is recently implicated in the pathogenesis of diabetic kidney injury. Celastrol exerts critical roles in inflammatory diseases and injury progression. However, its function and mechanism in DN remains elusive. Here, celastrol dose-dependently restored podocyte viability under high glucose (HG) conditions, but with little cytotoxicity in podocyte. Preconditioning with celastrol counteracted HG-evoked cell apoptosis, LDH release, ROS production and podocyte depletion. Additionally, HG-elevated high transcripts and secretions of pro-inflammatory cytokines were reversed following celastrol treatment, including IL-1β, TNF-α, IL-6. Simultaneously, the inhibitory effects of HG on insulin-triggered glucose uptake and nephrin expression were overturned after celastrol exposure. Intriguingly, celastrol restored HG-induced deficiency of autophagy pathway. Nevertheless, blocking the autophagy signaling by its antagonist 3-MA muted celastrol-protected against HG-evoked cell injury, inflammation and insulin resistance. Importantly, celastrol enhanced heme oxygenase-1 (HO-1) expression in HG-stimulated podocytes. Notably, HO-1 cessation depressed autophagy pathway activation and subsequently blunted beneficial effects of celastrol on HG-exposed podocytes. These finding suggest that celastrol may protect against HG-induced podocyte injury, inflammation and insulin resistance by restoring HO-1-mediated autophagy pathway, implying a promising therapeutic strategy against DN.

A novel laboratory-scale aerobic submerged membrane bioreactor integrating sponge-plastic biocarriers (SPSMBR) was conducted to study the removal and degradation mechanisms of sulfonamide antibiotics (SAs). Experimental results indicated that SPSMBR had a better removal of sulfadiazine (91% SDZ) and sulfamethoxazole (88% SMZ) than that of a conventional aerobic submerged membrane bioreactor (CSMBR) (76% SDZ and 71% SMZ, respectively). Material balance calculations suggested that biodegradation is the primary removal mechanism of SDZ and SMZ. Protein (tyrosine-like materials) significantly affected the removal of SAs. Moreover, the SPSMBR exhibited its better performance in removing SAs due to more abundance of tyrosine-like materials. The 16S rRNA sequencing showed that biocarriers could promote the enrichment of slow growing bacteria, especially Thermomonas, associated with the removal of SAs. Valuable insights into the removal and degradation mechanisms of SAs in the SPSMBR systems are documented here.

This study developed a new microbial fuel cell (MFC) system (Sponge-MFC), which consisted of a cathodic chamber with an added sponge and two anodic chambers, for low carbon/nitrogen (C/N) wastewater treatment. When operating in the closed-circuit state, the Sponge-MFC(C) demonstrated its superior electrochemical performance compared to the closed-circuit MFC. This superiority took the form of higher coulombic efficiencies, voltage outputs, current densities and power densities. Adding a sponge could reduce the cathode’s charge transfer resistance and solution resistance, and improve its capacitance, thus increasing cathodic reaction rate and power outputs. Simultaneous nitrification denitrification (SND) and bioelectrochemical denitrification processes on the cathode coupled with the sponge’s SND process were responsible for efficient removal of nitrogen from the Sponge-MFC(C). Fluorescent in situ hybridization (FISH) analysis revealed that nitrifying bacteria and highly diversified denitrifying bacteria were distributed at the cathode’s outer layer and inner layer, respectively. Higher phosphorus removal efficiencies (82.06 ± 1.21%) in the Sponge-MFC(C) than that in the MFC(C) (53.97 ± 2.32%) could be ascribed to biological phosphorus removal and precipitation of phosphate salts on the cathode. These results suggested the Sponge-MFC(C) could accomplish better electrochemical behaviors and nutrient removal due to sponge addition when treating wastewater with low C/N ratio.

The structural, electrical and mid-infrared optical properties of wurtzite structured ZnSe nanowires with different Chromium impurity distribution are investigated using first-principles calculation based on density-functional theory (DFT). The formation energies have been calculated to study the relative stabilities of different Cr doping positions. It is shown that when the Cr doping position shifted from the center to the edge, the splitting energy between 5T2 and 5E levels of Cr d-orbitals is decreased and a redshift is observed in the calculated infrared absorption spectra. A probable reason for these effects of the impurity distribution is discussed. Keywords: First-principles, Nanowires, Impurity distribution, Cr-doped ZnSe

The effect of powdered activated carbon (PAC) addition to a conventional anaerobic membrane bioreactor (AnMBR) on removal performance and membrane fouling was explored in this study. The optimal one-off PAC dose could increase average chemical oxygen demand (COD) and total organic carbon (TOC) removal rates up to 15.7% and 15.6%, respectively. The PAC addition exhibited not only lower TMP increase rate, but also reduced soluble microbial product (SMP) and extracellular polymeric substances (EPS). Higher protein to polysaccharide ratio of SMP and higher zeta potential in AnMBR with PAC could enhance hydrophobicity and flocculation ability, thereby effectively alleviating membrane fouling. Lower total membrane resistance and pore blocking resistance indicated that PAC addition could prevent both severe pore blocking and irreversible fouling, due to the lower polysaccharide level in SMP of the cake layer. Moreover, PAC addition facilitated the decrease in fouling-related bacteria such as Cloacibacterium and Paludibacter.

This study investigated the environmental burdens concerning the recycling/recovery process of a wastewater treatment plant's construction material waste and biogas. Detailed data inventories of case studies were employed in several scenarios to explore the role of end-of-life treatment methods. The ReCiPe 2016 and the Greenhouse gas Protocol life cycle impact methods were conducted to measure the impact categories. The construction and demolition phases were considered for recycling potential assessment, while the operational phase was examined for assessing the advantages of energy recovery. Metal and concrete recycling show environmental benefits. Increasing the reprocessing rate requires more water consumption but results in: firstly, a decrease of 18.8% in total damage; secondly, reduces problematic mineral scarcity by 3.9%; and thirdly, a shortfall in fossil fuels amounting to 11.6%. Recycling concrete helps to reduce the amount of GHG emissions 1.4-fold. Different biogas treatment methods contribute to various outcomes. Biogas utilization for on-site energy purposes has more advantages than flaring and offsite consumption. Electricity and heat generation originating from biogas can provide 70% of the energy requirement and replace 100% natural gas usage. Biomethane production from biogas requires extreme power and more resources. Meanwhile, producing heat and electricity can offset 102.9 g of fossil CO2, and manufacturing biomethane contributes the equivalent of 101.2 g of fossil fuel-derived CO2. Reducing 10% of recovered electricity creation could rise 19.19% global warming indicator of the wastewater treatment plant.

We demonstrate the synthesis of C–Cl-codoped titania/attapulgite (TiO2/ATT) composites containing a mixture of TiO2 phases by a facile sol-gel method at 70 °C using titanium tetraisopropoxide as the TiO2 precursor and ATT as a support for the TiO2 nanoparticles. The photocatalytic activity of the C–Cl-codoped TiO2/ATT composites with mixed anatase/brookite/rutile phases obtained at pH = 3.0 was much higher than that of commercially available Degussa P25 for the photocatalytic degradation of acid red G under visible-light irradiation. The excellent photocatalytic activity of the developed composite originates from the nonmetal codoping, which extends the absorption edge of TiO2 into visible region, and the presence of multiple phases, which slow the recombination of photoexcited electron/hole pairs. The formation of hydroxyl radicals during the photocatalytic degradation process was detected by photoluminescence probing using terephthalic acid. A mechanism for photocatalysis over the C–Cl-codoped TiO2/ATT composites was proposed.

In this study, a numerical model was introduced for simulation of preparation of TiO2 by non-premixed combustion of Ti particles in counter-flow arrangement. Preheating, reaction, melting, and vaporization processes were modeled using the asymptotic concept. To reach a viable model, convective and radiative heat losses were included in the model derivation. The conservation equations for mass and energy were presented in each zone with the heat loss terms. Boundary and jump conditions were employed to solve the governing equations to preserve continuity throughout the system. The numerical modeling was first validated against literature data, where good agreement was achieved. Then, the focus of results and discussion were given to the influence of heat loss terms on the spatial distributions of temperature and species mass fractions. It was clearly shown in that heat losses can significantly shorten the distances between the stagnation plane and flame fronts. This is the also the case for melting and vaporization fronts. However, the heat losses can obviously suppress thermophoretic force. Finally, the effects of initial fuel temperature on positions of flame, melting, and vaporization fronts, where almost linear dependences were found that higher initial fuel temperature results in closer fronts to the stagnation plane.

© 2016 Elsevier Ltd The anaerobic digestion process has been primarily utilized for methane containing biogas production over the past few years. However, the digestion process could also be optimized for producing volatile fatty acids (VFAs) and biohydrogen. This is the first review article that combines the optimization approaches for all three possible products from the anaerobic digestion. In this review study, the types and configurations of the bioreactor are discussed for each type of product. This is followed by a review on optimization of common process parameters (e.g. temperature, pH, retention time and organic loading rate) separately for the production of VFA, biohydrogen and methane. This review also includes additional parameters, treatment methods or special additives that wield a significant and positive effect on production rate and these products’ yield.

© 2016 Elsevier Ltd. In this study, new sponge modified plastic carriers for moving bed biofilm reactor (MBBR) was developed. The performance and membrane fouling behavior of a hybrid MBBR-membrane bioreactor (MBBR-MBR) system were also evaluated. Comparing to the MBBR with plastic carriers (MBBR), the MBBR with sponge modified biocarriers (S-MBBR) showed better effluent quality and enhanced nutrient removal at HRTs of 12 h and 6 h. Regarding fouling issue of the hybrid systems, soluble microbial products (SMP) of the MBR unit greatly influenced membrane fouling. The sponge modified biocarriers could lower the levels of SMP in mixed liquor and extracellular polymeric substances in activated sludge, thereby mitigating cake layer and pore blocking resistances of the membrane. The reduced SMP and biopolymer clusters in membrane cake layer were also observed. The results demonstrated that the sponge modified biocarriers were capable of improving overall MBBR performance and substantially alleviated membrane fouling of the subsequent MBR unit.

Potential energy curves and permanent dipole moments of the 6Σ+ and 8Σ+ ground state of CrX (X = Li, Na, K, Rb and Cs) are calculated by employing the complete active space self-consistent field (CASSCF) and multi-reference configuration interaction (MRCI) methods. The spectroscopic constants for the 6Σ+ and 8Σ+ ground state of these molecules are calculated. Moreover, CrK, CrRb and CrCs molecules with large values of permanent dipole moment (CrK: 5.553 D, CrRb: 6.341 D and CrCs: 6.731 D) at the equilibrium bond distance are potentially interesting candidates for ultracold anisotropic long-range dipole–dipole interactions and many-body physics studies.

Background : Therapeutic angiogenesis is a novel strategy for the treatment of ischemic diseases that involves promotion of angiogenesis in ischemic tissues via the use of proangiogenic agents. However, effective proangiogenic drugs that activate the Ang2/Tie2 signaling pathway remain scarce. Purpose : We aimed to investigate the proangiogenic activity of notoginsenoside R1 (NR1) isolated from total saponins of Panax notoginseng with regard to activation of the Ang2/Tie2 signaling pathway. Methods : We examined the proangiogenic effects of NR1 by assessing the effects of NR1 on the proliferation, migration, invasion and tube formation of human umbilical vein endothelial cells (HUVECs). An aortic ring assay and vascular endothelial growth factor receptor inhibitor (VRI)-induced vascular regression in a zebrafish model were used to confirm the proangiogenic effects of NR1 ex vivo and in vivo. Furthermore, the molecular mechanism was investigated by Western blot analysis. Results : We found that NR1 promoted the proliferation, mobility and tube formation of HUVECs in vitro. It also increased the number of sprouting vessels in rat aortic rings and rescued VRI-induced vascular regression in zebrafish. NR1-induced angiogenesis was dependent on Tie2 receptor activation mediated by increased autocrine Ang2 in HUVECs, and inhibition of the Ang2/Tie2 pathway abrogated the proangiogenic effects of NR1. Conclusions : Our results suggest that NR1 promotes angiogenesis by activating the Ang2/Tie2 signaling pathway. Thus, NR1-induced activation of the Ang2/Tie2 pathway is an effective proangiogenic approach. NR1 may be useful agent for the treatment of ischemic diseases.

No critical nitrogen (N) curve, used for diagnosing the in-season N status of crop plants, has been constructed for sweetpotato. Moreover, the critical root N curve has rarely been studied. This study determined three new critical N curves based on shoot (SDM), root (RDM), and plant (PDM) dry matter to assess N nutrition in sweetpotato in China. Three field experiments were conducted with varying N application rates (0–180 kg N ha–1) using two sweetpotato cultivars, Xinxiang and Shang 19. The relationship between dry matter and N concentration was analysed, and a model of critical N concentration for sweetpotato was constructed. Critical shoot N for sweetpotato based on SDM (Ns) is described by the equation Ns = 5.14 SDM–0.661 (SDM ≥ 1.7 t ha–1); for SDM

The feasibility of a novel bioflocculant (GemFloc™) for membrane fouling mitigation in membrane bioreactor (MBR) was investigated during real municipal wastewater treatment. When compared to the conventional MBR (CMBR), suspended sludge in the MBR with GemFloc™ (G-MBR) showed less soluble microbial products (SMP), higher ratios of proteins to polysaccharides in SMP (SMPP/SMPC) and loosely bound extracellular polymeric substances (LB-EPS). Adding GemFloc™ also enlarged floc size (> 200 µm), and increased tightly bound EPS levels, zeta potential and relative hydrophobicity of sludge flocs, further reduced cake layer and pore blocking resistances. Moreover, more diverse microbial community and enrichment of fouling reduction microbes such as Arenimonas and Flavihumibacter were observed in the G-MBR, together with less abundant microbes (e.g. Sphaerotilus and Povalibacter) which could aggravate membrane fouling. Therefore, GemFloc™ has high capability in improving sludge characteristics, mitigating membrane fouling and increasing diversity of special functional bacterial community in MBR.

Per- and polyfluoroalkyl substances (PFAS) are pollutants have attracted major concern due to their high persistence and bioaccumulation. They are causing increasingly serious epidemiological problems in many communities globally due to consuming PFAS-contaminated water sources. Necessarily, the behavior of PFAS in water and wastewater needs to be understood better. This study attempts to comprehensively review, analyze and discuss PFAS based on the following key aspects: (i) sources, (ii) occurrence in water and wastewater, (iii) transformation, fate and migration, and (iv) remediation technologies. Studies indicated that modern water and wastewater treatment plants cannot deal completely with PFAS and in some cases, the removal efficiency is minus -3500-fold. The main reasons are the high hydrophobicity of PFAS and presence of PFAS precursors. Precursors can account for 33–63% of total PFAS concentration in water and wastewater. Detection and identification of precursors are challenging due to the requirement of advanced analytical instrument and standard chemicals. Several technologies have been developed for PFAS remediation involving two main mechanisms: separation-concentration and destruction. The most widespread in-use technology is adsorption because it is reasonably affordable. Anion exchange resin and synthesized materials are the most effective sorbents having a sorption capacity of 100–2000 mg PFAS/g sorbent, effective within a few hours. The destruction technology such as plasma can also be a promising one for degrading PFAS to below health-based standard in 1 min. However, plasma is costly and not yet ready for full scale application.

We simulated rapid flow in transient plane Couette flows of granular particles using the smoothed particle hydrodynamics (SPH) solutions of a set of continuum equations. This simulation was performed to test the viability of SPH in solving the equations for the solid phase of the two-fluid model associated with fluidization. We found that SPH requires the handling of fewer particles in simulating the collective behavior of rapid granular flow, thereby bolstering expectations of solving the equations for the solid phase in the two-fluid modeling of fluidization. Further work is needed to investigate the effect of terms describing pressure and viscous stress of solids on stability in simulations. (C) 2014 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

Background Hepatocellular carcinoma (HCC) ranks third among the most common causes of cancer-related deaths worldwide. The chemotherapy for HCC is still insufficient, so far. In searching for effective anti-HCC agents from traditional Chinese medicine, we discovered that aloperine (ALO), a quinolizidine alkaloid from Sophora alopecuroides L., exerts anti-HCC activities. However, the effects of ALO on HCC have been rarely studied, and its underlying mechanisms remain unknown. Purpose This study aims to evaluate the anti-HCC activities of ALO and explore its underlying mechanisms. Methods MTT assay and colony formation assay were used to investigate the anti-proliferative effects of ALO on human HCC Hep3B and Huh7 cells. Hoechst 33258 staining was used to observe the morphological changes of cells after ALO treatment. Flow cytometry was used to analyze apoptosis induction, the collapse of the mitochondrial membrane potential and cell cycle distribution. Western blotting was used to examine the expression levels of proteins associated with apoptosis and cell cycle arrest, and key proteins in the PI3K/Akt signaling pathway. Small interfering RNA (siRNA) transfection was used to investigate the role of Akt in ALO-induced apoptosis and cell cycle arrest. Zebrafish tumor model was used to evaluate the anti-HCC effects of ALO in vivo. Results ALO inhibited the proliferation of Hep3B and Huh7 cells. ALO induced apoptosis in HCC cells, which was accompanied by the loss of mitochondrial potential, the release of cytochrome c into cytosol, as well as the increased cleavages of caspase-9, caspase-3 and PARP. Moreover, ALO induced G2/M cell cycle arrest by downregulating the expression levels of cdc25C, cdc2 and cyclin B1. In addition, ALO inhibited activation of the PI3K/Akt signaling pathway by decreasing the expression levels of p110α, p85, Akt and p-Akt (Ser473). Further study showed that inhibition of Akt by siRNA augmented ALO-mediated apoptosis and G2/M cell cycle arrest in HCC cells. Critically, ALO inhibited the growth of Huh7 cells in vivo. Conclusion We first demonstrated that ALO induced apoptosis and G2/M cell cycle arrest in HCC cells through inhibition of the PI3K/Akt signaling pathway. This study provides a rationale for ALO as a potential chemotherapeutic agent for HCC.

A two-fluid smoothed particle hydrodynamics (TF-SPH) method was proposed in this work for the simulation of gas solid fluidization, which combines an Eulerian solution of the gas phase and a Lagrangian SPH solution of the solid phase. The basic governing equations of TF-SPH are the same with those of the two-fluid model (TFM). The SPH solution of the solid phase allows significant reduction of the amount of particles that is actually simulated as compared to that encountered in industrial reactors. Thus, it can be expected to speed up the simulation. The sensitivity of SPH parameters to smoothing length and its ratio to particle space was tested. Quantitative comparison was also provided against the experimental data in literature. The TF-SPH method can be expected to be a promising approach for simulation of gas solid fluidization. (C) 2013 Elsevier Ltd. All rights reserved.

A selective and sensitive ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC–MS) method was developed for the simultaneous determination of ten biogenic amines (tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, adrenaline, dopamine and spermine) in a thymopolypeptides injection from the Chinese market for the first time. Biogenic amines (BAs) were pre-column derivatised by dansyl chloride after direct sample dilution. Dansylated amines were separated on an ACQUITY UPLC BEH Shield RP18 column (2.1 mm × 150 mm I.D., 1.7 μm) using a gradient elution. Quantification was done by monitoring fragment ions of each derivative under the MS mode of multiple reaction monitoring (MRM). A satisfactory result of method validation was obtained. The linearity ranged from 0.32 to 1182.9 μg/L and the correlation coefficients ( r ) for all amines were above 0.99. The LOD ranged from 0.08 μg/L for 2-phenylethylamine and tyramine to 8.00 μg/L for adrenaline; the LOQ ranged from 0.32 μg/L for 2-phenylethylamine to 12.12 μg/L for dopamine. The recovery ranged from 75.8 to 110.3% after spiking standard solutions of BAs to a sample at three levels. The intra and inter-day precision RSD were 0.78–8.85% and 1.39–9.93% respectively. Eighty-four injections were analyzed by this method. Nine biogenic amines were found in them except adrenaline. Moreover, the relationship between the result of test for depressor substances and the content of BAs was statistically analyzed.

With the dramatic development of computational science and technology, computer simulation is playing an increasingly important role in scientific research and engineering practice, and is believed to bring about a profound revolution to the mode and means of these activities. For chemical engineering, it will promote the transition from an experience-and-experiment-based research and development mode to the one based on virtual process engineering (VPE). However, such a revolutions till requires tremendous improvements in the Accuracy of physical modeling and numerical methods, the Capability of the computing hardware and software, and the Efficiency of the simulation activities, or in short, ACE. This article will systematically review the 3-decade endeavors at IPE, CAS(Institute of Process Engineering, Chinese Academy of Sciences) on upgrading ACE by establishing a multi-scale computing paradigm focusing on meso-scale structures. We also report recent developments in this direction with projections on future work.

The TiO 2 /rectorite composites with a bicrystalline (anatase and rutile) framework were synthesized by a facile sol–gel method using titanium tetraisopropoxide as a TiO 2 precursor at different temperatures. The as-obtained composites were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), UV–vis diffuse reflectance spectrum, and Brunauer–Emmett–Teller (BET). XRD and HRTEM analyses confirm that the layered structure of rectorite in the TiO 2 /rectorite composites was destroyed to some extent. The TiO 2 /rectorite composites prepared at low temperature (80 °C) without calcination exhibited strong adsorbility for acid red G (ARG), and showed a high photocatalytic activity for the decomposition of ARG and the persistent organic pollutant 4-nitrophenol (4-NP) under UV irradiation. The excellent photocatalytic activity of the as-obtained composites can be attributed to the synergetic effect of the anatase–rutile mixed phase and bimodal pore structure in the composites. A possible mechanism of the photocatalysis over the TiO 2 /rectorite composites was proposed.