1. Seeding depth and seeding rate regulate apical hook formation by inducing GhHLS1 expression via ethylene during cotton emergence
- Author
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Zhen Luo, Jingyuan Zhou, Hezhong Dong, Dongmei Zhang, Changle Ma, Weijiang Li, Lu Hequan, Xue Li, Hui Zhang, Xiangqiang Kong, and Wei Tang
- Subjects
0106 biological sciences ,0301 basic medicine ,Gossypium ,Ethylene ,Physiology ,Chemistry ,Arabidopsis ,Plant Science ,Ethylenes ,01 natural sciences ,Apical hook ,03 medical and health sciences ,Mechanical pressure ,Horticulture ,chemistry.chemical_compound ,030104 developmental biology ,Gene Expression Regulation, Plant ,Seedlings ,Gene expression ,Genetics ,Gene silencing ,Seeding ,010606 plant biology & botany - Abstract
Apical hook formation is essential for the emergence and stand establishment of cotton plants. Searching for agronomic measures to regulate apical hook formation and clarifying its mechanism are important for full stand establishment in cotton. In this study, cotton seeds were sown at varying seeding rates or depths in sand to determine if and how apical hook formation was regulated by seeding rates or depths. The results showed that deep seeding or low seeding rates increased mechanical pressure and then increased ethylene content by increasing GhACO1 and GhACS2 expression to improve apical hook formation. Silencing of the GhACO1 and GhACS2 genes or exogenous application of 1-methylcyclopropene (1-MCP) decreased the ethylene content and inhibited apical hook formation in the cotton seedlings. Deep seeding, a low seeding rate, or 1-amino cyclopropane-1-carboxylic acid (ACC) treatment increased the expression of GhHLS1 and GhPIF3 genes, but their expression was decreased in theVIGS-ACO1 and VIGS-ACS2 seedlings. Silencing of the GhHLS1 and GhPIF3 genes inhibited apical hook formation, although the expression of GhACO1 and GhACS2 was unchanged. GhPIF3 may act upstream of GhHLS1, as the expression of GhPIF3 in the VIGS-HLS1 seedlings was unchanged, while the expression of GhHLS1 in the VIGS-PIF3 seedlings decreased. These results suggested that raised mechanical pressure could increase ethylene content by inducing GhACO1 and GhACS2 gene expression, which promoted apical hook formation by increasing the expression of GhHLS1. Therefore, adjusting the mechanical pressure through changing the seeding depth or seeding rate is an important means to regulate apical hook formation and emergence.
- Published
- 2021
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