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The transcriptional co-activator YAP1 oncogene is the downstream effector of the Hippo pathway, which regulates tissue homeostasis, organ size, regeneration, and tumorigenesis. Multiple cancers are dependent on sustained expression of YAP1 for cell proliferation, survival, and tumorigenesis, but the molecular basis of this oncogene dependency is not well understood. To identify genes that can functionally substitute for YAP1, we performed a genome-scale genetic rescue screen in YAP1-dependent colon cancer cells expressing an inducible YAP1-specific shRNA. We found that the transcription factor PRDM14 rescued cell proliferation and tumorigenesis upon YAP1 suppression in YAP1-dependent cells, xenografts, and colon cancer organoids. YAP1 and PRDM14 individually activated the transcription of calmodulin 2 (CALM2) and a glucose transporter SLC2A1 upon YAP1 suppression, and CALM2 or SLC2A1 expression was required for the rescue of YAP1 suppression. Together, these findings implicate PRDM14-mediated transcriptional upregulation of CALM2 and SLC2A1 as key components of oncogenic YAP1 signaling and dependency.

This study aimed to identify any distinction between first-time and repeat convention attendees based on their perspectives of convention quality dimensions, multidimensional value, and behavioral intentions. The findings showed that accessibility and extra convention opportunities were not found to affect multidimensional value from first-timers and repeaters. First-timers valued professional education the most, followed by social networking and site environment in evaluating multidimensional value. Repeaters relied on social networking more than on education in assessing value. Site environment positively contributed to multidimensional value for first-timers but not for repeaters. Multidimensional value affected behavioral intentions more positively for repeaters than for first-timers. These findings will enable convention organizers to better understand the distinctions between the two groups and thus gain insight into how to attract first-timers and turn them into repeaters.

Cell-cell cooperativity in populations of motile and transiently interacting immune cells has been difficult to assess in the absence of tools to control proximity and communication. Here, we describe the generation of cell-laden microwells that can precisely control contact-mediated interactions and multicellular 'quorum' decisions in lymphocytes. Different types of fate decisions for activating T cells can be shown to variously obey 'binary' or 'density' outcomes, correlated with cell-cell contact, using this new platform.