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147 results on '"Moniuszko A"'

1. Global and risk-group stratified well-being and mental health during the COVID-19 pandemic in adults: Results from the international COH-FIT Study

Author

Solmi, Marco, primary, Thompson, Trevor, additional, Estradé, Andrés, additional, Agorastos, Agorastos, additional, Radua, Joaquim, additional, Cortese, Samuele, additional, Dragioti, Elena, additional, Leisch, Friedrich, additional, Vancampfort, Davy, additional, Thygesen, Lau Caspar, additional, Aschauer, Harald, additional, Schlögelhofer, Monika, additional, Aschauer, Elena, additional, Schneeberger, Andres, additional, Huber, Christian G., additional, Hasler, Gregor, additional, Conus, Philippe, additional, Cuénod, Kim Q. Do, additional, von Känel, Roland, additional, Arrondo, Gonzalo, additional, Fusar-Poli, Paolo, additional, Gorwood, Philip, additional, Llorca, Pierre-Michel, additional, Krebs, Marie-Odile, additional, Scanferla, Elisabetta, additional, Kishimoto, Taishiro, additional, Rabbani, Golam, additional, Skonieczna-Żydecka, Karolina, additional, Brambilla, Paolo, additional, Favaro, Angela, additional, Takamiya, Akihiro, additional, Zoccante, Leonardo, additional, Colizzi, Marco, additional, Bourgin, Julie, additional, Kamiński, Karol, additional, Moghadasin, Maryam, additional, Seedat, Soraya, additional, Matthews, Evan, additional, Wells, John, additional, Vassilopoulou, Emilia, additional, Gadelha, Ary, additional, Su, Kuan-Pin, additional, Kwon, Jun Soo, additional, Kim, Minah, additional, Lee, Tae Young, additional, Papsuev, Oleg, additional, Manková, Denisa, additional, Boscutti, Andrea, additional, Gerunda, Cristiano, additional, Saccon, Diego, additional, Righi, Elena, additional, Monaco, Francesco, additional, Croatto, Giovanni, additional, Cereda, Guido, additional, Demurtas, Jacopo, additional, Brondino, Natascia, additional, Veronese, Nicola, additional, Enrico, Paolo, additional, Politi, Pierluigi, additional, Ciappolino, Valentina, additional, Pfennig, Andrea, additional, Bechdolf, Andreas, additional, Meyer-Lindenberg, Andreas, additional, Kahl, Kai G., additional, Domschke, Katharina, additional, Bauer, Michael, additional, Koutsouleris, Nikolaos, additional, Winter, Sibylle, additional, Borgwardt, Stefan, additional, Bitter, Istvan, additional, Balazs, Judit, additional, Czobor, Pál, additional, Unoka, Zsolt, additional, Mavridis, Dimitris, additional, Tsamakis, Konstantinos, additional, Bozikas, Vasilios P., additional, Tunvirachaisakul, Chavit, additional, Maes, Michael, additional, Rungnirundorn, Teerayuth, additional, Supasitthumrong, Thitiporn, additional, Haque, Ariful, additional, Brunoni, Andre R., additional, Costardi, Carlos Gustavo, additional, Schuch, Felipe Barreto, additional, Polanczyk, Guilherme, additional, Luiz, Jhoanne Merlyn, additional, Fonseca, Lais, additional, Aparicio, Luana V., additional, Valvassori, Samira S., additional, Nordentoft, Merete, additional, Vendsborg, Per, additional, Hoffmann, Sofie Have, additional, Sehli, Jihed, additional, Sartorius, Norman, additional, Heuss, Sabina, additional, Guinart, Daniel, additional, Hamilton, Jane, additional, Kane, John, additional, Rubio, Jose, additional, Sand, Michael, additional, Koyanagi, Ai, additional, Solanes, Aleix, additional, Andreu-Bernabeu, Alvaro, additional, Cáceres, Antonia San José, additional, Arango, Celso, additional, Díaz-Caneja, Covadonga M., additional, Hidalgo-Mazzei, Diego, additional, Vieta, Eduard, additional, Gonzalez-Peñas, Javier, additional, Fortea, Lydia, additional, Parellada, Mara, additional, Fullana, Miquel A., additional, Verdolini, Norma, additional, Andrlíková, Eva, additional, Janků, Karolina, additional, Millan, Mark J., additional, Honciuc, Mihaela, additional, Moniuszko-Malinowska, Anna, additional, Łoniewski, Igor, additional, Samochowiec, Jerzy, additional, Kiszkiel, Łukasz, additional, Marlicz, Maria, additional, Sowa, Paweł, additional, Marlicz, Wojciech, additional, Spies, Georgina, additional, Stubbs, Brendon, additional, Firth, Joseph, additional, Sullivan, Sarah, additional, Darcin, Asli Enez, additional, Aksu, Hatice, additional, Dilbaz, Nesrin, additional, Noyan, Onur, additional, Kitazawa, Momoko, additional, Kurokawa, Shunya, additional, Tazawa, Yuki, additional, Anselmi, Alejandro, additional, Cracco, Cecilia, additional, Machado, Ana Inés, additional, Estrade, Natalia, additional, De Leo, Diego, additional, Curtis, Jackie, additional, Berk, Michael, additional, Carvalho, Andre F., additional, Ward, Philip, additional, Teasdale, Scott, additional, Rosenbaum, Simon, additional, Marx, Wolfgang, additional, Horodnic, Adrian Vasile, additional, Oprea, Liviu, additional, Alexinschi, Ovidiu, additional, Ifteni, Petru, additional, Turliuc, Serban, additional, Ciuhodaru, Tudor, additional, Bolos, Alexandra, additional, Matei, Valentin, additional, Nieman, Dorien H., additional, Sommer, Iris, additional, van Os, Jim, additional, van Amelsvoort, Therese, additional, Sun, Ching-Fang, additional, Guu, Ta-wei, additional, Jiao, Can, additional, Zhang, Jieting, additional, Fan, Jialin, additional, Zou, Liye, additional, Yu, Xin, additional, Chi, Xinli, additional, de Timary, Philippe, additional, van Winkel, Ruud, additional, Ng, Bernardo, additional, Pena, Edilberto, additional, Arellano, Ramon, additional, Roman, Raquel, additional, Sanchez, Thelma, additional, Movina, Larisa, additional, Morgado, Pedro, additional, Brissos, Sofia, additional, Aizberg, Oleg, additional, Mosina, Anna, additional, Krinitski, Damir, additional, Mugisha, James, additional, Sadeghi-Bahmani, Dena, additional, Sheybani, Farshad, additional, Sadeghi, Masoud, additional, Hadi, Samira, additional, Brand, Serge, additional, Errazuriz, Antonia, additional, Crossley, Nicolas, additional, Ristic, Dragana Ignjatovic, additional, López-Jaramillo, Carlos, additional, Efthymiou, Dimitris, additional, Kuttichira, Praveenlal, additional, Kallivayalil, Roy Abraham, additional, Javed, Afzal, additional, Afridi, Muhammad Iqbal, additional, James, Bawo, additional, Seb-Akahomen, Omonefe Joy, additional, Fiedorowicz, Jess, additional, Daskalakis, Jeff, additional, Yatham, Lakshmi N., additional, Yang, Lin, additional, Okasha, Tarek, additional, Dahdouh, Aïcha, additional, Gerdle, Björn, additional, Tiihonen, Jari, additional, Shin, Jae Il, additional, Lee, Jinhee, additional, Mhalla, Ahmed, additional, Gaha, Lotfi, additional, Brahim, Takoua, additional, Altynbekov, Kuanysh, additional, Negay, Nikolay, additional, Nurmagambetova, Saltanat, additional, Jamei, Yasser Abu, additional, Weiser, Mark, additional, and Correll, Christoph U., additional

Published
2024
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6. Validation of the Collaborative Outcomes study on Health and Functioning during Infection Times (COH-FIT) questionnaire for adults

Author

Solmi, Marco, primary, Thompson, Trevor, additional, Estradé, Andrés, additional, Agorastos, Agorastos, additional, Radua, Joaquim, additional, Cortese, Samuele, additional, Dragioti, Elena, additional, Leisch, Friedrich, additional, Vancampfort, Davy, additional, Thygesen, Lau Caspar, additional, Aschauer, Harald, additional, Schlögelhofer, Monika, additional, Aschauer, Elena, additional, Schneeberger, Andres, additional, Huber, Christian G., additional, Hasler, Gregor, additional, Conus, Philippe, additional, Do Cuénod, Kim Q., additional, von Känel, Roland, additional, Arrondo, Gonzalo, additional, Fusar-Poli, Paolo, additional, Gorwood, Philip, additional, Llorca, Pierre-Michel, additional, Krebs, Marie-Odile, additional, Scanferla, Elisabetta, additional, Kishimoto, Taishiro, additional, Rabbani, Golam, additional, Skonieczna-Żydecka, Karolina, additional, Brambilla, Paolo, additional, Favaro, Angela, additional, Takamiya, Akihiro, additional, Zoccante, Leonardo, additional, Colizzi, Marco, additional, Bourgin, Julie, additional, Kamiński, Karol, additional, Moghadasin, Maryam, additional, Seedat, Soraya, additional, Matthews, Evan, additional, Wells, John, additional, Vassilopoulou, Emilia, additional, Gadelha, Ary, additional, Su, Kuan-Pin, additional, Kwon, Jun Soo, additional, Kim, Minah, additional, Lee, Tae Young, additional, Papsuev, Oleg, additional, Manková, Denisa, additional, Boscutti, Andrea, additional, Gerunda, Cristiano, additional, Saccon, Diego, additional, Righi, Elena, additional, Monaco, Francesco, additional, Croatto, Giovanni, additional, Cereda, Guido, additional, Demurtas, Jacopo, additional, Brondino, Natascia, additional, Veronese, Nicola, additional, Enrico, Paolo, additional, Politi, Pierluigi, additional, Ciappolino, Valentina, additional, Pfennig, Andrea, additional, Bechdolf, Andreas, additional, Meyer-Lindenberg, Andreas, additional, Kahl, Kai G., additional, Domschke, Katharina, additional, Bauer, Michael, additional, Koutsouleris, Nikolaos, additional, Winter, Sibylle, additional, Borgwardt, Stefan, additional, Bitter, Istvan, additional, Balazs, Judit, additional, Czobor, Pál, additional, Unoka, Zsolt, additional, Mavridis, Dimitris, additional, Tsamakis, Konstantinos, additional, Bozikas, Vasilios P., additional, Tunvirachaisakul, Chavit, additional, Maes, Michael, additional, Rungnirundorn, Teerayuth, additional, Supasitthumrong, Thitiporn, additional, Haque, Ariful, additional, Brunoni, Andre R., additional, Costardi, Carlos Gustavo, additional, Schuch, Felipe Barreto, additional, Polanczyk, Guilherme, additional, Luiz, Jhoanne Merlyn, additional, Fonseca, Lais, additional, Aparicio, Luana V., additional, Valvassori, Samira S., additional, Nordentoft, Merete, additional, Vendsborg, Per, additional, Hoffmann, Sofie Have, additional, Sehli, Jihed, additional, Sartorius, Norman, additional, Heuss, Sabina, additional, Guinart, Daniel, additional, Hamilton, Jane, additional, Kane, John, additional, Rubio, Jose, additional, Sand, Michael, additional, Koyanagi, Ai, additional, Solanes, Aleix, additional, Andreu-Bernabeu, Alvaro, additional, Cáceres, Antonia San José, additional, Arango, Celso, additional, Díaz-Caneja, Covadonga M., additional, Hidalgo-Mazzei, Diego, additional, Vieta, Eduard, additional, Gonzalez-Peñas, Javier, additional, Fortea, Lydia, additional, Parellada, Mara, additional, Fullana, Miquel A., additional, Verdolini, Norma, additional, Andrlíková, Eva, additional, Janků, Karolina, additional, Millan, Mark John, additional, Honciuc, Mihaela, additional, Moniuszko-Malinowska, Anna, additional, Łoniewski, Igor, additional, Samochowiec, Jerzy, additional, Kiszkiel, Łukasz, additional, Marlicz, Maria, additional, Sowa, Paweł, additional, Marlicz, Wojciech, additional, Spies, Georgina, additional, Stubbs, Brendon, additional, Firth, Joseph, additional, Sullivan, Sarah, additional, Darcin, Asli Enez, additional, Aksu, Hatice, additional, Dilbaz, Nesrin, additional, Noyan, Onur, additional, Kitazawa, Momoko, additional, Kurokawa, Shunya, additional, Tazawa, Yuki, additional, Anselmi, Alejandro, additional, Cracco, Cecilia, additional, Machado, Ana Inés, additional, Estrade, Natalia, additional, De Leo, Diego, additional, Curtis, Jackie, additional, Berk, Michael, additional, Ward, Philip, additional, Teasdale, Scott, additional, Rosenbaum, Simon, additional, Marx, Wolfgang, additional, Horodnic, Adrian Vasile, additional, Oprea, Liviu, additional, Alexinschi, Ovidiu, additional, Ifteni, Petru, additional, Turliuc, Serban, additional, Ciuhodaru, Tudor, additional, Bolos, Alexandra, additional, Matei, Valentin, additional, Nieman, Dorien H., additional, Sommer, Iris, additional, van Os, Jim, additional, van Amelsvoort, Therese, additional, Sun, Ching-Fang, additional, Guu, Ta-wei, additional, Jiao, Can, additional, Zhang, Jieting, additional, Fan, Jialin, additional, Zou, Liye, additional, Yu, Xin, additional, Chi, Xinli, additional, de Timary, Philippe, additional, van Winkel, Ruud, additional, Ng, Bernardo, additional, Pena, Edilberto, additional, Arellano, Ramon, additional, Roman, Raquel, additional, Sanchez, Thelma, additional, Movina, Larisa, additional, Morgado, Pedro, additional, Brissos, Sofia, additional, Aizberg, Oleg, additional, Mosina, Anna, additional, Krinitski, Damir, additional, Mugisha, James, additional, Sadeghi-Bahmani, Dena, additional, Sheybani, Farshad, additional, Sadeghi, Masoud, additional, Hadi, Samira, additional, Brand, Serge, additional, Errazuriz, Antonia, additional, Crossley, Nicolas, additional, Ristic, Dragana Ignjatovic, additional, López-Jaramillo, Carlos, additional, Efthymiou, Dimitris, additional, Kuttichira, Praveenlal, additional, Kallivayalil, Roy Abraham, additional, Javed, Afzal, additional, Afridi, Muhammad Iqbal, additional, James, Bawo, additional, Seb-Akahomen, Omonefe Joy, additional, Fiedorowicz, Jess, additional, Carvalho, Andre F., additional, Daskalakis, Jeff, additional, Yatham, Lakshmi N., additional, Yang, Lin, additional, Okasha, Tarek, additional, Dahdouh, Aïcha, additional, Gerdle, Björn, additional, Tiihonen, Jari, additional, Shin, Jae Il, additional, Lee, Jinhee, additional, Mhalla, Ahmed, additional, Gaha, Lotfi, additional, Brahim, Takoua, additional, Altynbekov, Kuanysh, additional, Negay, Nikolay, additional, Nurmagambetova, Saltanat, additional, Jamei, Yasser Abu, additional, Weiser, Mark, additional, and Correll, Christoph U., additional

Published
2023
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7. Implementation of the User-Friendly Odds Ratio Calculator for Unvaccinated Individuals in a Country with a High COVID-19 Death Toll

Author

Miroslaw Kwasniewski, Urszula Korotko, Karolina Chwialkowska, Magdalena Niemira, Jerzy Jaroszewicz, Barbara Sobala-Szczygiel, Beata Puzanowska, Anna Moniuszko-Malinowska, Sławomir Pancewicz, Anna Parfieniuk-Kowerda, Diana Martonik, Dorota Zarębska-Michaluk, Krzysztof Simon, Monika Pazgan-Simon, Iwona Mozer-Lisewska, Maciej Bura, Agnieszka Adamek, Krzysztof Tomasiewicz, Małgorzata Pawłowska, Anna Piekarska, Aleksandra Berkan-Kawińska, Andrzej Horban, Justyna Kowalska, Regina Podlasin, Piotr Wasilewski, Arsalin Azzadin, Miroslaw Czuczwar, Michal Borys, Pawel Piwowarczyk, Slawomir Czaban, Jacek Bogocz, Magdalena Ochab, Anna Kruk, Sandra Uszok, Agnieszka Bielska, Anna Szalkowska, Justyna Raczkowska, Gabriela Sokolowska, Joanna Chorostowska-Wynimko, Aleksandra Jezela-Stanek, Adroana Rozy, Urszula Lechowicz, Urszula Polowianiuk, Agnieszka Tycinska, Kamil Grubczak, Aleksandra Starosz, Wiktoria Izdebska, Tadeusz Faustyn Krzeminski, Jean Bousquet, Milena Sokolowska, Genoveffa Franchini, Jennifer Hadlock, Adam Kretowski, Andrzej Eljaszewicz, Robert Flisiak, and Marcin Moniuszko

Subjects
History, Polymers and Plastics, Business and International Management, Industrial and Manufacturing Engineering
Published
2022
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9. Implementation of the User-Friendly Odds Ratio Calculator for Unvaccinated Individuals in a Country with a High COVID-19 Death Toll

Author

Kwasniewski, Miroslaw, primary, Korotko, Urszula, additional, Chwialkowska, Karolina, additional, Niemira, Magdalena, additional, Jaroszewicz, Jerzy, additional, Sobala-Szczygiel, Barbara, additional, Puzanowska, Beata, additional, Moniuszko-Malinowska, Anna, additional, Pancewicz, Sławomir, additional, Parfieniuk-Kowerda, Anna, additional, Martonik, Diana, additional, Zarębska-Michaluk, Dorota, additional, Simon, Krzysztof, additional, Pazgan-Simon, Monika, additional, Mozer-Lisewska, Iwona, additional, Bura, Maciej, additional, Adamek, Agnieszka, additional, Tomasiewicz, Krzysztof, additional, Pawłowska, Małgorzata, additional, Piekarska, Anna, additional, Berkan-Kawińska, Aleksandra, additional, Horban, Andrzej, additional, Kowalska, Justyna, additional, Podlasin, Regina, additional, Wasilewski, Piotr, additional, Azzadin, Arsalin, additional, Czuczwar, Miroslaw, additional, Borys, Michal, additional, Piwowarczyk, Pawel, additional, Czaban, Slawomir, additional, Bogocz, Jacek, additional, Ochab, Magdalena, additional, Kruk, Anna, additional, Uszok, Sandra, additional, Bielska, Agnieszka, additional, Szalkowska, Anna, additional, Raczkowska, Justyna, additional, Sokolowska, Gabriela, additional, Chorostowska-Wynimko, Joanna, additional, Jezela-Stanek, Aleksandra, additional, Rozy, Adroana, additional, Lechowicz, Urszula, additional, Polowianiuk, Urszula, additional, Tycinska, Agnieszka, additional, Grubczak, Kamil, additional, Starosz, Aleksandra, additional, Izdebska, Wiktoria, additional, Krzeminski, Tadeusz Faustyn, additional, Bousquet, Jean, additional, Sokolowska, Milena, additional, Franchini, Genoveffa, additional, Hadlock, Jennifer, additional, Kretowski, Adam, additional, Eljaszewicz, Andrzej, additional, Flisiak, Robert, additional, and Moniuszko, Marcin, additional

Published
2022
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10. Assessment of tick-borne pathogens presence in Dermacentor reticulatus ticks in north-eastern Poland

Author

Adam Trzeszczkowski, Justyna Dunaj, Sławomir Pancewicz, Krzysztof Rutkowski, and Anna Moniuszko-Malinowska

Subjects
Bartonella, Ixodes ricinus, animal diseases, Tick, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Dermacentor reticulatus, Bartonella Infections, parasitic diseases, Animals, Humans, Medicine, 030212 general & internal medicine, Rickettsia, Dermacentor, Lyme Disease, biology, business.industry, Ehrlichiosis, Rickettsia Infections, General Medicine, bacterial infections and mycoses, biology.organism_classification, Coxiella burnetii, Anaplasma phagocytophilum, Tick-Borne Diseases, Borrelia burgdorferi, 030220 oncology & carcinogenesis, Babesia, Babesia canis, bacteria, Poland, Q Fever, business
Abstract

Purpose Dermacentor reticulatus is the second most common tick species in Poland after Ixodes ricinus. The aim of the study was to analyze the presence of pathogen DNA in D. reticulatus. Materials and methods Ticks were collected in The Protected Landscape Area of the Bug and Nurzec Valley (52°40′ N and 22°28’ E) between 2016 and 2017. End-point PCR for Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Babesia spp., Rickettsia spp., Bartonella spp. and Coxiella burnetii detection was performed. Results Tick-borne pathogens’ DNA was detected in 11.3% of 301 ticks: B. burgdorferi s.l. in 3.6%, Babesia spp. in 6.3%, A. phagocytophilum in 0.7% and B. burgdorferi s.l.-Babesia spp. co-infection in 0.7%. In all 21 Babesia spp. positive samples, sequence analysis confirmed the presence of Babesia canis with an 80.3%–98.3% homology with the B. canis sequences in GenBank. C. burnetii, Bartonella spp., and Rickettsia spp. DNA were not detected. Conclusions Dermacentor reticulatus from north-eastern Poland were found to carry three of the most common tick-borne pathogens (B. burgdorferi s.l., Babesia canis, A. phagocytophilum) which lead to single and mixed infections. Babesia canis was the most prevalent pathogen identified in D. reticulatus.

Published
2021
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12. Inflammatory and thrombotic parameters associated with the COVID-19 course in Poland (SARSTer study)

Author

Czupryna, Piotr, primary, Moniuszko-Malinowska, Anna, additional, Rogalska, Magdalena, additional, Zarębska-Michaluk, Dorota, additional, Lorenc, Beata, additional, Rorat, Marta, additional, Kozielewicz, Dorota, additional, Tudrujek-Zdunek, Magdalena, additional, Sikorska, Katarzyna, additional, Berkan-Kawińska, Aleksandra, additional, Bolewska, Beata, additional, Mazur, Włodzimierz, additional, Kowalska, Justyna, additional, Oczko-Grzesik, Barbara, additional, Podlasin, Regina, additional, Piekarska, Anna, additional, Kłos, Krzysztof, additional, Szymczak, Aleksandra, additional, Leszczyński, Piotr, additional, Maciejewska, Katarzyna, additional, Pancewicz, Sławomir, additional, Jaroszewicz, Jerzy, additional, Milewski, Robert, additional, Groth, Monika, additional, and Flisiak, Robert, additional

Published
2022
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13. The association of airborne particulate matter and benzo[a]pyrene with the clinical course of COVID-19 in patients hospitalized in Poland

Author

Rzymski, Piotr, primary, Poniedziałek, Barbara, additional, Rosińska, Joanna, additional, Rogalska, Magdalena, additional, Zarębska-Michaluk, Dorota, additional, Rorat, Marta, additional, Moniuszko-Malinowska, Anna, additional, Lorenc, Beata, additional, Kozielewicz, Dorota, additional, Piekarska, Anna, additional, Sikorska, Katarzyna, additional, Dworzańska, Anna, additional, Bolewska, Beata, additional, Angielski, Grzegorz, additional, Kowalska, Justyna, additional, Podlasin, Regina, additional, Oczko-Grzesik, Barbara, additional, Mazur, Włodzimierz, additional, Szymczak, Aleksandra, additional, and Flisiak, Robert, additional

Published
2022
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14. New Treatment of Wound Healing With Allogenic Acellular Human Skin Graft: Preclinical Assessment and In Vitro Study

Author

Piotr Fiedor, Jacek Pliszczyński, Magdalena Nita, Dariusz Śladowski, Slawomir Majewski, Marcin Moniuszko, Cezary Kowalewski, Artur Kamiński, Andrzej Eljaszewicz, Zbigniew Zimek, Maciej Kosieradzki, and Katarzyna Woźniak

Subjects
Acellular Dermis, Pathology, medicine.medical_specialty, Human skin, In Vitro Techniques, medicine, Humans, Transplantation, Homologous, Basement membrane, Wound Healing, Transplantation, Biological Dressings, Tissue Engineering, integumentary system, business.industry, Multipotent Stem Cells, Skin Transplantation, medicine.disease, medicine.anatomical_structure, Multipotent Stem Cell, Surgery, Epidermolysis bullosa, Stem cell, business, Wound healing
Abstract

Background Nonhealing wounds can be a major clinical problem. Impaired wound healing is often related to massive tissue injury, concomitant wound healing deficiencies (chronic wounds), burn injury, or congenital conditions. We propose a novel biological dressing as an alternative surgical approach. The dressing is a form of an allogenic human skin graft equivalent with further use of allogeneic stem cells classified as an advanced therapy medicinal product. This new allogenic acellular human skin graft has been specifically developed to address the clinical indications for dressing wound lesions and promoting tissue repair in specific rare genetic diseases. Methods This case report illustrates the use of an acellular human skin allograft seeded with multipotent stem cells in the treatment of tissue injuries (burns), congenital conditions, and chronic wounds. Donor-tissue processing yields an acellular dermal matrix with integral collagen bundling and organization, as well as an intact basement membrane complex. Results Preclinical observations show prolonged viability of acellular human skin grafts with multipotent stem cells. This was confirmed with histological and electron-microscopic evaluation of biopsies, which demonstrated host-cell infiltration and neovascularization of the biological dressing. Moreover, the dressings were characterized by low immunogenicity, as confirmed by histology exam and T-cell proliferation assays in vitro. Conclusion Our data confirmed the safety and efficacy of the evaluated acellular human skin grafts, which may be used in patients with rare diseases, such as epidermolysis bullosa, burn injuries, and chronic wounds.

Published
2020
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15. T102 Has the SARS-COV-2 pandemic affected cardiovascular risk parameters in the general population?

Author

Chlabicz, M., primary, Jamiołkowski, J., additional, Szum-Jakubowska, A., additional, Sowa, P., additional, Dubatówka, M., additional, Łapińska, M., additional, Kondraciuk, M., additional, Motyka, J., additional, Chlabicz, M., additional, Szpakowicz, A., additional, Ławicki, S., additional, Moniuszko-Malinowska, A., additional, and Kamiński, K., additional

Published
2022
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16. The first SARS-CoV-2 genetic variants of concern (VOC) in Poland: The concept of a comprehensive approach to monitoring and surveillance of emerging variants

Author

Charkiewicz, Radosław, primary, Nikliński, Jacek, additional, Biecek, Przemysław, additional, Kiśluk, Joanna, additional, Pancewicz, Sławomir, additional, Moniuszko-Malinowska, Anna M., additional, Flisiak, Robert, additional, Krętowski, Adam J., additional, Dzięcioł, Janusz, additional, Moniuszko, Marcin, additional, Gierczyński, Rafał, additional, Juszczyk, Grzegorz, additional, and Reszeć, Joanna, additional

Published
2021
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18. The collaborative outcomes study on health and functioning during infection times in adults (COH-FIT-Adults): Design and methods of an international online survey targeting physical and mental health effects of the COVID-19 pandemic

Author

Solmi, Marco, primary, Estradé, Andrés, additional, Thompson, Trevor, additional, Agorastos, Agorastos, additional, Radua, Joaquim, additional, Cortese, Samuele, additional, Dragioti, Elena, additional, Leisch, Friedrich, additional, Vancampfort, Davy, additional, Thygesen, Lau Caspar, additional, Aschauer, Harald, additional, Schloegelhofer, Monika, additional, Akimova, Elena, additional, Schneeberger, Andres, additional, Huber, Christian G., additional, Hasler, Gregor, additional, Conus, Philippe, additional, Cuénod, Kim Q. Do, additional, von Känel, Roland, additional, Arrondo, Gonzalo, additional, Fusar-Poli, Paolo, additional, Gorwood, Philip, additional, Llorca, Pierre-Michel, additional, Krebs, Marie-Odile, additional, Scanferla, Elisabetta, additional, Kishimoto, Taishiro, additional, Rabbani, Golam, additional, Skonieczna-Żydecka, Karolina, additional, Brambilla, Paolo, additional, Favaro, Angela, additional, Takamiya, Akihiro, additional, Zoccante, Leonardo, additional, Colizzi, Marco, additional, Bourgin, Julie, additional, Kamiński, Karol, additional, Moghadasin, Maryam, additional, Seedat, Soraya, additional, Matthews, Evan, additional, Wells, John, additional, Vassilopoulou, Emilia, additional, Gadelha, Ary, additional, Su, Kuan-Pin, additional, Kwon, Jun Soo, additional, Kim, Minah, additional, Lee, Tae Young, additional, Papsuev, Oleg, additional, Manková, Denisa, additional, Boscutti, Andrea, additional, Gerunda, Cristiano, additional, Saccon, Diego, additional, Righi, Elena, additional, Monaco, Francesco, additional, Croatto, Giovanni, additional, Cereda, Guido, additional, Demurtas, Jacopo, additional, Brondino, Natascia, additional, Veronese, Nicola, additional, Enrico, Paolo, additional, Politi, Pierluigi, additional, Ciappolino, Valentina, additional, Pfennig, Andrea, additional, Bechdolf, Andreas, additional, Meyer-Lindenberg, Andreas, additional, Kahl, Kai G., additional, Domschke, Katharina, additional, Bauer, Michael, additional, Koutsouleris, Nikolaos, additional, Winter, Sibylle, additional, Borgwardt, Stefan, additional, Bitter, Istvan, additional, Balazs, Judit, additional, Czobor, Pal, additional, Unoka, Zsolt, additional, Mavridis, Dimitris, additional, Tsamakis, Konstantinos, additional, Bozikas, Vasilios P., additional, Tunvirachaisakul, Chavit, additional, Maes, Michael, additional, Rungnirundorn, Teerayuth, additional, Supasitthumrong, Thitiporn, additional, Haque, Ariful, additional, Brunoni, Andre R., additional, Costardi, Carlos Gustavo, additional, Schuch, Felipe Barreto, additional, Polanczyk, Guilherme, additional, Luiz, Jhoanne Merlyn, additional, Fonseca, Lais, additional, Aparicio, Luana V., additional, Valvassori, Samira S., additional, Nordentoft, Merete, additional, Vendsborg, Per, additional, Hoffmann, Sofie Have, additional, Sehli, Jihed, additional, Sartorius, Norman, additional, Heuss, Sabina, additional, Guinart, Daniel, additional, Hamilton, Jane, additional, Kane, John, additional, Rubio, Jose, additional, Sand, Michael, additional, Koyanagi, Ai, additional, Solanes, Aleix, additional, Andreu-Bernabeu, Alvaro, additional, Cáceres, Antonia San José, additional, Arango, Celso, additional, Díaz-Caneja, Covadonga M., additional, Hidalgo-Mazzei, Diego, additional, Vieta, Eduard, additional, Gonzalez-Peñas, Javier, additional, Fortea, Lydia, additional, Parellada, Mara, additional, Fullana, Miquel A., additional, Verdolini, Norma, additional, Fárková, Eva, additional, Janků, Karolina, additional, Millan, Mark, additional, Honciuc, Mihaela, additional, Moniuszko-Malinowska, Anna, additional, Łoniewski, Igor, additional, Samochowiec, Jerzy, additional, Kiszkiel, Łukasz, additional, Marlicz, Maria, additional, Sowa, Paweł, additional, Marlicz, Wojciech, additional, Spies, Georgina, additional, Stubbs, Brendon, additional, Firth, Joseph, additional, Sullivan, Sarah, additional, Darcin, Asli Enez, additional, Aksu, Hatice, additional, Dilbaz, Nesrin, additional, Noyan, Onur, additional, Kitazawa, Momoko, additional, Kurokawa, Shunya, additional, Tazawa, Yuki, additional, Anselmi, Alejandro, additional, Cracco, Cecilia, additional, Machado, Ana Inés, additional, Estrade, Natalia, additional, De Leo, Diego, additional, Curtis, Jackie, additional, Berk, Michael, additional, Ward, Philip, additional, Teasdale, Scott, additional, Rosenbaum, Simon, additional, Marx, Wolfgang, additional, Horodnic, Adrian Vasile, additional, Oprea, Liviu, additional, Alexinschi, Ovidiu, additional, Ifteni, Petru, additional, Turliuc, Serban, additional, Ciuhodaru, Tudor, additional, Bolos, Alexandra, additional, Matei, Valentin, additional, Nieman, Dorien H., additional, Sommer, Iris, additional, van Os, Jim, additional, van Amelsvoort, Therese, additional, Sun, Ching-Fang, additional, Guu, Ta-wei, additional, Jiao, Can, additional, Zhang, Jieting, additional, Fan, Jialin, additional, Zou, Liye, additional, Yu, Xin, additional, Chi, Xinli, additional, de Timary, Philippe, additional, van Winke, Ruud, additional, Ng, Bernardo, additional, Pena, Edilberto, additional, Arellano, Ramon, additional, Roman, Raquel, additional, Sanchez, Thelma, additional, Movina, Larisa, additional, Morgado, Pedro, additional, Brissos, Sofia, additional, Aizberg, Oleg, additional, Mosina, Anna, additional, Krinitski, Damir, additional, Mugisha, James, additional, Sadeghi-Bahmani, Dena, additional, Sadeghi, Masoud, additional, Hadi, Samira, additional, Brand, Serge, additional, Errazuriz, Antonia, additional, Crossley, Nicolas, additional, Ristic, Dragana Ignjatovic, additional, López-Jaramillo, Carlos, additional, Efthymiou, Dimitris, additional, Kuttichira, Praveenlal, additional, Kallivayalil, Roy Abraham, additional, Javed, Afzal, additional, Afridi, Muhammad Iqbal, additional, James, Bawo, additional, Seb-Akahomen, Omonefe Joy, additional, Fiedorowicz, Jess, additional, Carvalho, Andre F., additional, Daskalakis, Jeff, additional, Yatham, Lakshmi N., additional, Yang, Lin, additional, Okasha, Tarek, additional, Dahdouh, Aïcha, additional, Gerdle, Björn, additional, Tiihonen, Jari, additional, Shin, Jae Il, additional, Lee, Jinhee, additional, Mhalla, Ahmed, additional, Gaha, Lotfi, additional, Brahim, Takoua, additional, Altynbekov, Kuanysh, additional, Negay, Nikolay, additional, Nurmagambetova, Saltanat, additional, Jamei, Yasser Abu, additional, Weiser, Mark, additional, and Correll, Christoph U., additional

Published
2022
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19. Physical and mental health impact of COVID-19 on children, adolescents, and their families: The Collaborative Outcomes study on Health and Functioning during Infection Times - Children and Adolescents (COH-FIT-C&A)

Author

Solmi, Marco, primary, Estradé, Andrés, additional, Thompson, Trevor, additional, Agorastos, Agorastos, additional, Radua, Joaquim, additional, Cortese, Samuele, additional, Dragioti, Elena, additional, Leisch, Friedrich, additional, Vancampfort, Davy, additional, Thygesen, Lau Caspar, additional, Aschauer, Harald, additional, Schloegelhofer, Monika, additional, Akimova, Elena, additional, Schneeberger, Andres, additional, Huber, Christian G., additional, Hasler, Gregor, additional, Conus, Philippe, additional, Cuénod, Kim Q. Do, additional, von Känel, Roland, additional, Arrondo, Gonzalo, additional, Fusar-Poli, Paolo, additional, Gorwood, Philip, additional, Llorca, Pierre-Michel, additional, Krebs, Marie-Odile, additional, Scanferla, Elisabetta, additional, Kishimoto, Taishiro, additional, Rabbani, Golam, additional, Skonieczna-Żydecka, Karolina, additional, Brambilla, Paolo, additional, Favaro, Angela, additional, Takamiya, Akihiro, additional, Zoccante, Leonardo, additional, Colizzi, Marco, additional, Bourgin, Julie, additional, Kamiński, Karol, additional, Moghadasin, Maryam, additional, Seedat, Soraya, additional, Matthews, Evan, additional, Wells, John, additional, Vassilopoulou, Emilia, additional, Gadelha, Ary, additional, Su, Kuan-Pin, additional, Kwon, Jun Soo, additional, Kim, Minah, additional, Lee, Tae Young, additional, Papsuev, Oleg, additional, Manková, Denisa, additional, Boscutti, Andrea, additional, Gerunda, Cristiano, additional, Saccon, Diego, additional, Righi, Elena, additional, Monaco, Francesco, additional, Croatto, Giovanni, additional, Cereda, Guido, additional, Demurtas, Jacopo, additional, Brondino, Natascia, additional, Veronese, Nicola, additional, Enrico, Paolo, additional, Politi, Pierluigi, additional, Ciappolino, Valentina, additional, Pfennig, Andrea, additional, Bechdolf, Andreas, additional, Meyer-Lindenberg, Andreas, additional, Kahl, Kai G., additional, Domschke, Katharina, additional, Bauer, Michael, additional, Koutsouleris, Nikolaos, additional, Winter, Sibylle, additional, Borgwardt, Stefan, additional, Bitter, Istvan, additional, Balazs, Judit, additional, Czobor, Pal, additional, Unoka, Zsolt, additional, Mavridis, Dimitris, additional, Tsamakis, Konstantinos, additional, Bozikas, Vasilios P., additional, Tunvirachaisakul, Chavit, additional, Maes, Michael, additional, Rungnirundorn, Teerayuth, additional, Supasitthumrong, Thitiporn, additional, Haque, Ariful, additional, Brunoni, Andre R., additional, Costardi, Carlos Gustavo, additional, Schuch, Felipe Barreto, additional, Polanczyk, Guilherme, additional, Luiz, Jhoanne Merlyn, additional, Fonseca, Lais, additional, Aparicio, Luana V., additional, Valvassori, Samira S., additional, Nordentoft, Merete, additional, Vendsborg, Per, additional, Hoffmann, Sofie Have, additional, Sehli, Jihed, additional, Sartorius, Norman, additional, Heuss, Sabina, additional, Guinart, Daniel, additional, Hamilton, Jane, additional, Kane, John, additional, Rubio, Jose, additional, Sand, Michael, additional, Koyanagi, Ai, additional, Solanes, Aleix, additional, Andreu-Bernabeu, Alvaro, additional, Cáceres, Antonia San José, additional, Arango, Celso, additional, Díaz-Caneja, Covadonga M., additional, Hidalgo-Mazzei, Diego, additional, Vieta, Eduard, additional, Gonzalez-Peñas, Javier, additional, Fortea, Lydia, additional, Parellada, Mara, additional, Fullana, Miquel A., additional, Verdolini, Norma, additional, Fárková, Eva, additional, Janků, Karolina, additional, Millan, Mark, additional, Honciuc, Mihaela, additional, Moniuszko-Malinowska, Anna, additional, Łoniewski, Igor, additional, Samochowiec, Jerzy, additional, Kiszkiel, Łukasz, additional, Marlicz, Maria, additional, Sowa, Paweł, additional, Marlicz, Wojciech, additional, Spies, Georgina, additional, Stubbs, Brendon, additional, Firth, Joseph, additional, Sullivan, Sarah, additional, Darcin, Asli Enez, additional, Aksu, Hatice, additional, Dilbaz, Nesrin, additional, Noyan, Onur, additional, Kitazawa, Momoko, additional, Kurokawa, Shunya, additional, Tazawa, Yuki, additional, Anselmi, Alejandro, additional, Cracco, Cecilia, additional, Machado, Ana Inés, additional, Estrade, Natalia, additional, De Leo, Diego, additional, Curtis, Jackie, additional, Berk, Michael, additional, Ward, Philip, additional, Teasdale, Scott, additional, Rosenbaum, Simon, additional, Marx, Wolfgang, additional, Horodnic, Adrian Vasile, additional, Oprea, Liviu, additional, Alexinschi, Ovidiu, additional, Ifteni, Petru, additional, Turliuc, Serban, additional, Ciuhodaru, Tudor, additional, Bolos, Alexandra, additional, Matei, Valentin, additional, Nieman, Dorien H., additional, Sommer, Iris, additional, van Os, Jim, additional, van Amelsvoort, Therese, additional, Sun, Ching-Fang, additional, Guu, Ta-wei, additional, Jiao, Can, additional, Zhang, Jieting, additional, Fan, Jialin, additional, Zou, Liye, additional, Yu, Xin, additional, Chi, Xinli, additional, de Timary, Philippe, additional, van Winke, Ruud, additional, Ng, Bernardo, additional, Pena, Edilberto, additional, Arellano, Ramon, additional, Roman, Raquel, additional, Sanchez, Thelma, additional, Movina, Larisa, additional, Morgado, Pedro, additional, Brissos, Sofia, additional, Aizberg, Oleg, additional, Mosina, Anna, additional, Krinitski, Damir, additional, Mugisha, James, additional, Sadeghi-Bahmani, Dena, additional, Sadeghi, Masoud, additional, Hadi, Samira, additional, Brand, Serge, additional, Errazuriz, Antonia, additional, Crossley, Nicolas, additional, Ristic, Dragana Ignjatovic, additional, López-Jaramillo, Carlos, additional, Efthymiou, Dimitris, additional, Kuttichira, Praveenlal, additional, Kallivayalil, Roy Abraham, additional, Javed, Afzal, additional, Afridi, Muhammad Iqbal, additional, James, Bawo, additional, Seb-Akahomen, Omonefe Joy, additional, Fiedorowicz, Jess, additional, Carvalho, Andre F., additional, Daskalakis, Jeff, additional, Yatham, Lakshmi N., additional, Yang, Lin, additional, Okasha, Tarek, additional, Dahdouh, Aïcha, additional, Gerdle, Björn, additional, Tiihonen, Jari, additional, Shin, Jae Il, additional, Lee, Jinhee, additional, Mhalla, Ahmed, additional, Gaha, Lotfi, additional, Brahim, Takoua, additional, Altynbekov, Kuanysh, additional, Negay, Nikolay, additional, Nurmagambetova, Saltanat, additional, Jamei, Yasser Abu, additional, Weiser, Mark, additional, and Correll, Christoph U., additional

Published
2022
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20. Loss of regulatory capacity in Treg cells following rhinovirus infection

Author

Jansen, Kirstin, primary, Wirz, Oliver F., additional, van de Veen, Willem, additional, Tan, Ge, additional, Mirer, David, additional, Sokolowska, Milena, additional, Satitsuksanoa, Pattraporn, additional, Message, Simon D., additional, Kebadze, Tatiana, additional, Glanville, Nicholas, additional, Mallia, Patrick, additional, Skiepko, Roman, additional, Eljaszewicz, Andrzej, additional, Moniuszko, Marcin, additional, Cardoso, Carlos, additional, Gern, James E., additional, Papadopoulos, Nikolaos G., additional, Akdis, Cezmi A., additional, Johnston, Sebastian L., additional, Nadeau, Kari C., additional, and Akdis, Mübeccel, additional

Published
2021
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21. The promoting effect of neutrophil-derived BAFF molecule on the proliferation and life span of CAL-27 oral squamous carcinoma cells

Author

Ewa, Jablonska, Agnieszka, Iwaniuk, Wioletta, Ratajczak-Wrona, Kamil, Grubczak, Dorota, Dziemianczyk-Pakiela, Marcin, Moniuszko, Karolina, Nowak, Jan, Borys, and Marzena, Garley

Subjects
Flavonoids, Neutrophils, Longevity, Immunology, Antineoplastic Agents, Apoptosis, Hematology, Transforming Growth Factor beta, Cell Line, Tumor, B-Cell Activating Factor, Carcinoma, Squamous Cell, Tumor Microenvironment, Humans, Immunology and Allergy, Mouth Neoplasms, Apoptosis Regulatory Proteins, Cell Proliferation
Abstract

Tumor-associated neutrophils (TANs) are the major cellular component of the tumor microenvironment and have been shown to release of different bioactive molecules such as B-cell activating factor (BAFF). The data on the interactions between OSCC cells and neutrophils are limited and do not explain the actual role of the BAFF in the development of the OSCC. In the present study we examined the direct effect of neutrophils-derived BAFF on the OSCC cell line CAL-27 proliferation and apoptosis. PMNs of OSCC patients and healthy control were isolated from whole blood and separated by magnetic selection with monoclonal anti-human CD16 antibodies. CD-16 - positive neutrophils were incubated in the presence of TGF-β and/or LPS as well as flavonoids (luteolin and quercetin). CAL-27 cells were co-incubated with supernatants of neutrophils. BAFF expression in neutrophils, BAFF-R expression on CAL-27 cells and apoptosis of CAL-27 cells were assessed by flow cytometry. To determine the CAL-27 cells proliferation, the MTT test was used. Expression of select mitochondrial proteins in CAL-27 cells were measured by Western blot. Neutrophils from OSCC patients showed significantly higher expression of BAFF than those from the healthy controls. The results obtained revealed upregulation of the proliferation and downregulation of the apoptosis of the CAL-27 cells in the presence of the supernatants of TGF-β-treated neutrophils. Flavonoids reduced BAFF expression in neutrophils of patients with OSCC and control group. Lower intensity of apoptosis in CAL-27 cells was associated with the increased expression of anti-apoptotic Bcl-2, Mcl-1 and activated form of PI3K kinase (pPI3K) and simultaneously reduced expression of pro-apoptotic Bax protein in the presence of rhBAFF, as well as of supernatants of neutrophils derived from OSCC patients. In conclusion, the data presented confirm the previously suggested role of neutrophil-derived BAFF in OSCC development. The favorable effects of examined flavonoids on tumor-promoting BAFF expression in neutrophils suggest that they might be promising candidates as chemo-preventive agents in the therapy of patients with OSCC.

Published
2022
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22. The association of airborne particulate matter and benzo[a]pyrene with the clinical course of COVID-19 in patients hospitalized in Poland

Author

Piotr Rzymski, Barbara Poniedziałek, Joanna Rosińska, Magdalena Rogalska, Dorota Zarębska-Michaluk, Marta Rorat, Anna Moniuszko-Malinowska, Beata Lorenc, Dorota Kozielewicz, Anna Piekarska, Katarzyna Sikorska, Anna Dworzańska, Beata Bolewska, Grzegorz Angielski, Justyna Kowalska, Regina Podlasin, Barbara Oczko-Grzesik, Włodzimierz Mazur, Aleksandra Szymczak, and Robert Flisiak

Subjects
Adult, Air Pollutants, Health, Toxicology and Mutagenesis, COVID-19, Environmental Exposure, General Medicine, Toxicology, Pollution, Hospitalization, Air Pollution, Benzo(a)pyrene, Humans, Particulate Matter, Poland
Abstract

Air pollution can adversely affect the immune response and increase the severity of the viral disease. The present study aimed to explore the relationship between symptomatology, clinical course, and inflammation markers of adult patients with coronavirus disease 2019 (COVID-19) hospitalized in Poland (n = 4432) and air pollution levels, i.e., mean 24 h and max 24 h level of benzo(a)pyrene (B(a)P) and particulate matter10 μm (PM

Published
2022
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23. Anaplasmosis in Poland – analysis of 120 patients

Author

Moniuszko-Malinowska, Anna, primary, Dunaj, Justyna, additional, Andersson, Martin O., additional, Chmielewski, Tomasz, additional, Czupryna, Piotr, additional, Groth, Monika, additional, Grygorczuk, Sambor, additional, Zajkowska, Joanna, additional, Kondrusik, Maciej, additional, Kruszewska, Ewelina, additional, and Pancewicz, Sławomir, additional

Published
2021
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25. Markers of anaphylaxis – a systematic review

Author

Agnieszka Lis, Anna Bodzenta-Łukaszyk, Maciej Klimek, Maria Magdalena Tomasiak-Łozowska, and Marcin Moniuszko

Subjects
0301 basic medicine, Tryptase, Stimulus (physiology), 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, medicine, Humans, Platelet, Platelet Activating Factor, Anaphylaxis, Platelet-activating factor, biology, business.industry, General Medicine, Mast cell, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, chemistry, Immunology, biology.protein, Tryptases, Differential diagnosis, business, Biomarkers, Histamine, 030215 immunology
Abstract

Anaphylaxis is defined as severe, life-threatening, systemic or general, immediate reaction of hypersensitivity, with repeatable symptoms caused by the dose of stimulus which is well tolerated by healthy persons. The proper diagnosis, immediate treatment and differential diagnosis are crucial for saving patient's life. However, anaphylaxis is relatively frequently misdiagnosed or confused with other clinical entities. Thus, there is a continuous need for identifying detectable markers improving the proper diagnosis of anaphylaxis. Here we presented currently known markers of anaphylaxis and discussed in more detail the most clinically valuable ones: tryptase, platelet activacting factor (PAF), PAF-acethylhydrolase, histamine and its metabolites.

Published
2018
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26. Intrathecal expression of IL-5 and humoral response in patients with tick-borne encephalitis

Author

Joanna Zajkowska, Justyna Dunaj, Sambor Grygorczuk, Anna Moniuszko-Malinowska, Piotr Czupryna, Renata Świerzbińska, Sławomir Pancewicz, and Maciej Kondrusik

Subjects
Adult, Male, 0301 basic medicine, Adolescent, Leukocytosis, Enzyme-Linked Immunosorbent Assay, Biology, Antibodies, Viral, Microbiology, Immunoglobulin G, Encephalitis Viruses, Tick-Borne, Serology, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Blood serum, medicine, Humans, Prospective Studies, Pleocytosis, Aged, Retrospective Studies, Inflammation, B-Lymphocytes, Antibody titer, Tick-borne encephalitis, Middle Aged, medicine.disease, Antibodies, Anti-Idiotypic, Immunity, Humoral, 030104 developmental biology, Infectious Diseases, Immunoglobulin M, Insect Science, Immunology, biology.protein, Female, Parasitology, Interleukin-5, Encephalitis, Tick-Borne, 030217 neurology & neurosurgery, Encephalitis
Abstract

Aim The aim of the study was to assess the role of an early specific humoral response in human infection with a tick-borne encephalitis virus (TBEV) and the role of IL-5 as its potential mediator and marker. Materials and methods The retrospective study involved a cohort of 199 patients diagnosed with TBE, in whom anti-TBEV IgM and IgG antibody titers were analyzed on admission and compared with clinical presentation and basic laboratory parameters. The prospective study included 50 TBE patients in whom IL-5 serum and CSF concentration was measured with ELISA on admission in the TBE neurologic phase and in selected patients before discharge, at follow-up or in samples obtained before the neurologic phase onset. Results The serum anti-TBEV IgM correlated with good clinical outcome and the CSF anti-TBEV IgM with more pronounced CSF inflammation on admission, but also with its more complete resolution on follow-up. The serum anti-TBEV IgG correlated with milder presentation and better outcome. Concentration of IL-5 was increased in CSF but not in the serum of TBE patients. IL-5 concentration index on admission favored its intrathecal synthesis. IL-5 did not correlate significantly with clinical presentation and specific IgM and IgG titers. Conclusions Specific anti-TBEV IgM systemic and intrathecal response and IgG systemic response are protective, together favoring milder presentation, better outcome and resolution of central nervous system (CNS) inflammation. IL-5 is expressed intrathecally in TBE, but its pathogenetic role remains unclear.

Published
2018
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28. Trained immunity and tolerance in innate lymphoid cells, monocytes, and dendritic cells during allergen-specific immunotherapy

Author

Eljaszewicz, Andrzej, primary, Ruchti, Fiorella, additional, Radzikowska, Urszula, additional, Globinska, Anna, additional, Boonpiyathad, Tadech, additional, Gschwend, Anna, additional, Morita, Hideaki, additional, Helbling, Arthur, additional, Arasi, Stefania, additional, Kahlert, Helga, additional, Berek, Nadine, additional, Nandy, Andreas, additional, Akdis, Mübeccel, additional, Willers, Christoph, additional, Moniuszko, Marcin, additional, Akdis, Cezmi A., additional, and Sokolowska, Milena, additional

Published
2021
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32. The lymphocyte populations and their migration into the central nervous system in tick-borne encephalitis

Author

Grygorczuk, Sambor, primary, Osada, Joanna, additional, Toczyłowski, Kacper, additional, Sulik, Artur, additional, Czupryna, Piotr, additional, Moniuszko-Malinowska, Anna, additional, Kondrusik, Maciej, additional, Świerzbińska, Renata, additional, Dunaj, Justyna, additional, Pancewicz, Sławomir, additional, and Dąbrowska, Milena, additional

Published
2020
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36. Tick-borne infections and co-infections in patients with non-specific symptoms in Poland

Author

Sławomir Pancewicz, Joanna Zajkowska, Krzysztof Rutkowski, Maciej Kondrusik, Izabela Swiecicka, Justyna Dunaj, Grzegorz Zambrowski, Sambor Grygorczuk, Anna Moniuszko-Malinowska, Piotr Czupryna, Martin O. Andersson, and Renata Świerzbińska

Subjects
Adult, Male, 0301 basic medicine, 030106 microbiology, Tick, Serology, 03 medical and health sciences, parasitic diseases, Humans, Medicine, Borrelia burgdorferi, Whole blood, biology, Coinfection, business.industry, Sequence Analysis, DNA, General Medicine, bacterial infections and mycoses, biology.organism_classification, Anaplasma phagocytophilum, Tick-Borne Diseases, Immunology, Babesia, Vomiting, Female, Poland, Headaches, medicine.symptom, business
Abstract

Aim The aim of the study was the evaluation of the frequency of infections and co-infections among patients hospitalized because of non-specific symptoms after a tick bite. Materials and methods Whole blood, serum and cerebrospinal fluid samples from 118 patients hospitalised for non-specific symptoms up to 8 weeks after tick bite from 2010 to 2013 were examined for tick-borne infections. ELISA, Western blot and/or molecular biology (PCR; fla gene; 16S rRNA; sequencing) and thin blood smears (MDD) were used. Control group included 50 healthy blood donors. All controls were tested with PCR and serology according to the same procedure as in patients. Results Out of 118 patients 85 (72%) experienced headaches, 15 (13%) vertigo, 32 (27%) nausea, 17 (14%) vomiting, 37 (31%) muscle pain, 73 (62%) fever and 26 (22%) meningeal signs. 47.5% were infected with at least one tick-borne pathogen. Borrelia burgdorferi sensu lato infection was confirmed with ELISA, Western blot in serum and/or (PCR (fla gene) in whole blood in 29.7% cases. In blood of 11.9% patients Anaplasma phagocytophilum DNA (16S rRNA gene) was detected; in 0.9% patients 1/118 Babesia spp. DNA (18S rRNA gene) was also detected. Co-infections were observed in 5.1% of patients with non-specific symptoms. B. burgdorferi s.l. − A. phagocytophilum co-infection (5/118; 4.2%) was most common. In 1/118 (0.8%) A. phagocytophilum – Babesia spp. co-infection was detected. All controls were negative for examined pathogens. Conclusions Non-specific symptoms after tick bite may be caused by uncommon pathogens or co-infection, therefore it should be considered in differential diagnosis after tick bite.

Published
2018
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37. Systematic biobanking, novel imaging techniques, and advanced molecular analysis for precise tumor diagnosis and therapy: The Polish MOBIT project

Author

Rodryg Ramlau, Barbara Mroczko, Bogusław Kędra, Magdalena Paczkowska, Anetta Sulewska, Lothar R. Pilz, Radoslaw Charkiewicz, Jolanta Malyszko, Zenon Mariak, Christian Manegold, Robert Mróz, Jacek Niklinski, Dorota Jurgilewicz, Magdalena Niemira, Adam Kretowski, Michal Ciborowski, Joanna Reszeć, Lukasz Minarowski, Monika M. Kaczmarek, Miroslaw Kwasniewski, Angelika Edyta Charkiewicz, Miroslaw Kozlowski, Heike Allgayer, Cezary Piwkowski, Anna Michalska-Falkowska, Marcin Moniuszko, Joanna Kisluk, Ewa Sierko, Mohammed Abba, Pawel Kurzawa, Jacek Szamatowicz, Adam Cybulski, Wojciech Naumnik, Andrzej Ciereszko, Hartmut Juhl, Frauke Koch, and Tomasz Wasniewski

Subjects
0301 basic medicine, medicine.medical_specialty, Proteome, Genomics, Disease, Bioinformatics, 03 medical and health sciences, 0302 clinical medicine, Predictive Value of Tests, Neoplasms, Biomarkers, Tumor, medicine, Humans, Medical physics, Molecular Targeted Therapy, Precision Medicine, Biological Specimen Banks, business.industry, Cancer, General Medicine, Precision medicine, medicine.disease, Biobank, Molecular Imaging, Molecular analysis, 030104 developmental biology, 030220 oncology & carcinogenesis, Metabolome, Identification (biology), Personalized medicine, business
Abstract

Personalized and precision medicine is gaining recognition due to the limitations by standard diagnosis and treatment; many areas of medicine, from cancer to psychiatry, are moving towards tailored and individualized treatment for patients based on their clinical characteristics and genetic signatures as well as novel imaging techniques. Advances in whole genome sequencing have led to identification of genes involved in a variety of diseases. Moreover, biomarkers indicating severity of disease or susceptibility to treatment are increasingly being characterized. The continued identification of new genes and biomarkers specific to disease subtypes and individual patients is essential and inevitable for translation into personalized medicine, in estimating both, disease risk and response to therapy. Taking into consideration the mostly unsolved necessity of tailored therapy in oncology the innovative project MOBIT (molecular biomarkers for individualized therapy) was designed. The aims of the project are: (i) establishing integrative management of precise tumor diagnosis and therapy including systematic biobanking, novel imaging techniques, and advanced molecular analysis by collecting comprehensive tumor tissues, liquid biopsies (whole blood, serum, plasma), and urine specimens (supernatant; sediment) as well as (ii) developing personalized lung cancer diagnostics based on tumor heterogeneity and integrated genomics, transcriptomics, metabolomics, and radiomics PET/MRI analysis. It will consist of 5 work packages. In this paper the rationale of the Polish MOBIT project as well as its design is presented. (iii) The project is to draw interest in and to invite national and international, private and public, preclinical and clinical initiatives to establish individualized and precise procedures for integrating novel targeted therapies and advanced imaging techniques.

Published
2017
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38. Gene Expression Signature Differentiates Histology But Not Progression Status of Early-Stage NSCLC

Author

Anetta Sulewska, Wieslawa Niklinska, Joanna Reszeć, Jacek Niklinski, Anna Michalska-Falkowska, Jürgen Claesen, Radoslaw Charkiewicz, Miroslaw Kozlowski, Wojciech Naumnik, and Marcin Moniuszko

Subjects
0301 basic medicine, Oncology, Original article, Cancer Research, medicine.medical_specialty, Biology, Bioinformatics, lcsh:RC254-282, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, Text mining, Internal medicine, Gene expression, medicine, Gene, business.industry, Histology, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, Gene expression profiling, 030104 developmental biology, 030220 oncology & carcinogenesis, Gene chip analysis, Adenocarcinoma, business
Abstract

Advances in molecular analyses based on high-throughput technologies can contribute to a more accurate classification of non-small cell lung cancer (NSCLC), as well as a better prediction of both the disease course and the efficacy of targeted therapies. Here we set out to analyze whether global gene expression profiling performed in a group of early-stage NSCLC patients can contribute to classifying tumor subtypes and predicting the disease prognosis. Gene expression profiling was performed with the use of the microarray technology in a training set of 108 NSCLC samples. Subsequently, the recorded findings were validated further in an independent cohort of 44 samples. We demonstrated that the specific gene patterns differed significantly between lung adenocarcinoma (AC) and squamous cell lung carcinoma (SCC) samples. Furthermore, we developed and validated a novel 53-gene signature distinguishing SCC from AC with 93% accuracy. Evaluation of the classifier performance in the validation set showed that our predictor classified the AC patients with 100% sensitivity and 88% specificity. We revealed that gene expression patterns observed in the early stages of NSCLC may help elucidate the histological distinctions of tumors through identification of different gene-mediated biological processes involved in the pathogenesis of histologically distinct tumors. However, we showed here that the gene expression profiles did not provide additional value in predicting the progression status of the early-stage NSCLC. Nevertheless, the gene expression signature analysis enabled us to perform a reliable subclassification of NSCLC tumors, and it can therefore become a useful diagnostic tool for a more accurate selection of patients for targeted therapies. This work was supported by the National Science Centre [grant no. NN401 3785 39], Leading National Research Centre (KNOW) [Cancer/Mutagenesis research area]. Equipment used in this study was purchased by the Medical University of Bialystok as part of the OP DEP 2007-2013, Priority Axis I.3, Contract No. POPW.01.03.00-20-022/09.

Published
2017
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39. Bufadienolides from Kalanchoe daigremontiana as thrombin inhibitors— In vitro and in silico study

Author

Anna Stochmal, Joanna Kolodziejczyk-Czepas, Barbara Moniuszko-Szajwaj, Łukasz Pecio, Michal B. Ponczek, Pawel Nowak, and Malgorzata Sieradzka

Subjects
Kalanchoe, 0301 basic medicine, Protein Conformation, Bufadienolide, 030204 cardiovascular system & hematology, Fibrinogen, Biochemistry, Argatroban, Fibrin, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Thrombin, Structural Biology, medicine, Computer Simulation, Protease Inhibitors, Molecular Biology, biology, General Medicine, biology.organism_classification, Amides, Bufanolides, Molecular Docking Simulation, 030104 developmental biology, chemistry, Coagulation, Proteolysis, biology.protein, medicine.drug, Discovery and development of direct thrombin inhibitors
Abstract

Thrombin is an active plasma coagulation factor II, critical for the formation of fibrin clot during blood coagulation. For that reason, this protein is also a crucial target for different anti-thrombotic therapies. The work is based on in vitro evaluation of the inhibitory effect of bufadienolide-rich fraction, isolated from roots of Kalanchoe daigremontiana (1-50μg/ml) on enzymatic properties of a serine proteinase - thrombin. The efficacy of the inhibition of amidolytic activity of thrombin (measured as a hydrolysis of the chromogenic substrate S-2238, Chromogenix) attained about 10 and 66%, respectively. The IC50, established for the examined bufadienolide fraction was 2.79μg/ml, while the IC50 calculated for argatroban (reference compound) was 0.78μg/ml. Linearization conducted using Lineweaver-Burk plot indicated that the K. daigremontiana fraction contains compounds that are uncompetitive inhibitors of thrombin. K. daigremontiana fraction was also able to reduce the proteolytic activity of thrombin towards its physiological substrate, i.e. fibrinogen. Additionally, this study is supported by in silico analysis of interactions of the most common compounds, identified in the examined in Kalanchoe extract to crystal structure of this enzyme.

Published
2017
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40. Anaplasmosis in Poland – analysis of 120 patients

Author

Joanna Zajkowska, Ewelina Kruszewska, Justyna Dunaj, Sambor Grygorczuk, Anna Moniuszko-Malinowska, Piotr Czupryna, Martin O. Andersson, Maciej Kondrusik, Tomasz Chmielewski, Monika Groth, and Sławomir Pancewicz

Subjects
Adult, Male, 0301 basic medicine, Bartonella, Anaplasmosis, 030231 tropical medicine, Biology, Tick, Microbiology, Serology, Young Adult, 03 medical and health sciences, Ticks, 0302 clinical medicine, parasitic diseases, medicine, Animals, Humans, Borrelia burgdorferi, Aged, Coinfection, Incidence, Middle Aged, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Virology, Anaplasma phagocytophilum, 030104 developmental biology, Infectious Diseases, Tick-Borne Diseases, Insect Science, Babesia, bacteria, Female, Parasitology, Poland, Encephalitis
Abstract

The aim of our study was to clarify the clinical picture of anaplasmosis through analysis of the symptoms and clinical signs presented by infected patients in a cohort of tick-bitten individuals. The study included 1375 patients with suspicion of tick-borne disease. Finally, 120 patients (8.7%) were diagnosed with anaplasmosis (HGA). Blood samples were examined by PCR for Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis, Borrelia burgdorferi sensu lato, Babesia spp., and Bartonella spp.. Based on analysis of 120 patients with HGA we concluded that anaplasmosis is not as rare in Europe, as it is thought to be and often appears as a co-infection with other tick-borne pathogens. The co-infection rate of patients with A. phagocytophilum infection in tick endemic areas is high. Co-infection of A. phagocytophilum with B. burgdorferi s.l. or tick-borne encephalitis virus may influence symptom frequency. PCR together with medical history, clinical picture and basic laboratory tests is a sufficient method for the diagnosis of anaplasmosis. Doxycycline is an effective drug leading to complete recovery.

Published
2021
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41. The chemotactic cytokines in the cerebrospinal fluid of patients with neuroborreliosis

Author

Justyna Dunaj, Renata Świerzbińska, Sławomir Pancewicz, Sambor Grygorczuk, Anna Moniuszko-Malinowska, and Piotr Czupryna

Subjects
Adult, Male, 0301 basic medicine, Leukocytosis, medicine.medical_treatment, Lymphocyte, Immunology, Lymphocytic pleocytosis, Biochemistry, CCL5, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Cerebrospinal fluid, stomatognathic system, CSF pleocytosis, immune system diseases, medicine, Humans, Lyme Neuroborreliosis, Immunology and Allergy, Pleocytosis, Molecular Biology, Aged, business.industry, Hematology, Middle Aged, medicine.disease, stomatognathic diseases, 030104 developmental biology, Cytokine, medicine.anatomical_structure, Blood-Brain Barrier, Erythema, 030220 oncology & carcinogenesis, CXCL9, Female, Chemokines, business, Follow-Up Studies
Abstract

Background The outcome of neuroborreliosis (NB) is variable and may partially depend on host-related immune factors. In NB, the cerebrospinal fluid (CSF) contains a large population of T lymphocytes, but the mechanisms and consequences of their recruitment have not been fully elucidated. We have studied expression of T lymphocyte chemoattractant cytokines in association with CSF cytometric parameters and clinical data in NB patients. Methods The blood and CSF of 17 patients with NB and blood of 12 patients with erythema migrans (EM) were obtained before the antibiotic administration, and in fraction of NB patients during and/or after antibiotic treatment. The control samples came from blood donors (blood) and patients in whom neuroinfection was excluded by a lumbar puncture (CSF). Concentrations of IL-16, CXCL9, CXCL10, CXCL11, CCL2 and CCL5 in serum and CSF were measured with commercial ELISA. Data were analyzed with non-parametric tests, p Results The serum concentrations of IL-16, CXCL9, CXCL10 and CCL5 were increased, higher in NB than in EM. In CSF all the cytokines were upregulated, CXCL10, CXCL9 and IL-16 over ten-fold. The CSF concentration index favored the intrathecal synthesis of all the cytokines except CCL5, for which it could not be reliably estimated. CCL2, CXCL10 and CXCL9 created concentration gradients towards CSF. The intrathecal expression of IL-16, CCL5 and CXCL9 correlated with CSF lymphocyte counts, of IL-16, CXCL9 and CXCL10 - with a blood-brain barrier disruption, and of CXCL9 and CXCL10 with intrathecal specific IgG synthesis. The expression of CCL2, CXCL10 and CXCL11 peaked early after NB onset and decreased naturally afterwards. High initial CSF CXCL9, CXCL10 and CXCL11 levels associated with a persistent CSF pleocytosis and BBB disruption after treatment, but no cytokine was predictive of clinical outcome. In follow up (post-treatment) examinations, CSF CXCL10 and CCL5 associated positively and CCL2 negatively with a protracted lymphocytic pleocytosis. Conclusions Several cytokines chemotactic for T lymphocytes are upregulated intrathecally in NB, with different dynamics and relation to other inflammatory parameters, suggesting their distinct pathogenetic roles. CXCL10 and CXCL9 are vividly upregulated and seem deeply involved in the pathogenesis of the intrathecal inflammation. IL-16 and CCL5 may directly drive T lymphocyte migration from periphery, but their ability to create an adequate chemotactic gradient remains to be confirmed. A delayed normalization of pleocytosis is accompanied by higher intrathecal expression of Th1-related and lower of Th2-related chemokines, in agreement with the protective role of Th1 to Th2 transition in the course of NB.

Published
2021
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42. The lymphocyte populations and their migration into the central nervous system in tick-borne encephalitis

Author

Justyna Dunaj, Artur Sulik, Renata Świerzbińska, Maciej Kondrusik, Sławomir Pancewicz, Kacper Toczylowski, Milena Dąbrowska, Joanna Osada, Sambor Grygorczuk, Anna Moniuszko-Malinowska, and Piotr Czupryna

Subjects
Central Nervous System, 0301 basic medicine, Lymphocyte, 030231 tropical medicine, Biology, Microbiology, CCL5, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, medicine, Humans, Pleocytosis, Tick-borne encephalitis, Meningoencephalitis, medicine.disease, Lymphocyte Subsets, CCL20, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Insect Science, Immunology, Parasitology, Meningitis, Encephalitis, Tick-Borne, Encephalitis
Abstract

In tick-borne encephalitis (TBE) the cerebrospinal fluid (CSF) cytosis is dominated by T CD3+CD4+ and T CD3+CD8+ lymphocytes, but their pathogenetic roles and mechanisms of migration into central nervous system (CNS) are unclear. Currently, we have studied CSF lymphocyte subsets and chemotactic axes in TBE patients stratified according to the clinical presentation. Blood and CSF were obtained from 51 patients with TBE (presenting as meningitis in 30, meningoencephalitis in 18 and meningoencephalomyelitis in 3), 20 with non-TBE meningitis and 11 healthy controls. We have studied: (1) abundances of the main lymphocyte subsets and (2) CXCR3 and CCR5 expression on CD3+CD4+ and CD3+CD8+ lymphocytes cytometrically with fluorochrome-stained monoclonal antibodies; (3) concentrations of chemotactic cytokines: CCL5 (CCR5 ligand), CXCL10 (CXCR3 ligand), IL-16, CCL2, CCL20 and CXCL5 with ELISA. Cytokine concentrations were additionally studied in 8 pediatric TBE patients. Data were analyzed with non-parametric tests, p 0.05 considered significant. The higher CSF lymphocyte counts were associated with symptoms of CNS involvement, especially with altered consciousness (B, Th and Tc cells) and focal neurologic deficits (B cells). The minor fraction of double-positive T CD4+CD8+ cells was unique in associating negatively with encephalitis and altered consciousness. CSF CD3+CD4+ and CD3+CD8+ lymphocyte population was enriched in CCR5-positive cells and CCL5 concentration in CSF was increased and associated with a milder presentation. Although CXCL10 was vividly up-regulated intrathecally and correlated with CSF T lymphocyte counts, the CXCR3 expression in CSF T lymphocytes was low. Serum and CSF concentrations of CCL2, CXCL5 and IL-16 were increased in adult TBE patients, CCL2 created a chemotactic gradient towards CSF and both CCL2 and IL-16 concentrations correlated positively with CSF lymphocyte counts. The particular lymphoid cell populations in CSF associate differently with the clinical presentation of TBE, suggesting their distinct roles in pathogenesis. CCR5/CCL5 axis probably contributes to T lymphocyte migration into CNS. CXCL10 mediates the intrathecal immune response, but is probably not directly responsible for T cell migration. Additional chemotactic factors must be involved, probably including CCL2 and IL-16.

Published
2020
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44. Analysis of CCL-4, CCL-17, CCL-20 and IL-8 concentrations in the serum of patients with tick-borne encephalitis and anaplasmosis

Author

Guziejko, Katarzyna, primary, Czupryna, Piotr, additional, Pancewicz, Sławomir, additional, Świerzbińska, Renata, additional, Dunaj, Justyna, additional, Kruszewska, Ewelina, additional, Król, Monika Emilia, additional, Zajkowska, Joanna, additional, Grygorczuk, Sambor, additional, Żebrowska, Agnieszka, additional, Kondrusik, Maciej, additional, and Moniuszko-Malinowska, Anna, additional

Published
2020
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46. Expression of serine proteases in neutrophils from women and men: Regulation by endocrine disruptor bisphenol A

Author

Ratajczak-Wrona, Wioletta, primary, Nowak, Karolina, additional, Garley, Marzena, additional, Grubczak, Kamil, additional, Dabrowska, Dorota, additional, Iwaniuk, Agnieszka, additional, Wilk, Sara, additional, Moniuszko, Marcin, additional, Czerniecki, Jan, additional, Wolczynski, Slawomir, additional, and Jablonska, Ewa, additional

Published
2019
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49. Increased expression of Fas receptor and Fas ligand in the culture of the peripheral blood mononuclear cells stimulated with Borrelia burgdorferi sensu lato

Author

Sławomir Pancewicz, Joanna Osada, Joanna Zajkowska, Justyna Dunaj, Milena Dąbrowska, Renata Świerzbińska, Anna Moniuszko, Maciej Kondrusik, and Sambor Grygorczuk

Subjects
Adult, Fas Ligand Protein, Population, Apoptosis, Lymphocyte Activation, Microbiology, Peripheral blood mononuclear cell, Fas ligand, Young Adult, Transforming Growth Factor beta, parasitic diseases, medicine, Animals, Humans, fas Receptor, Borrelia burgdorferi, education, Aged, Aged, 80 and over, Lyme Disease, education.field_of_study, biology, Middle Aged, bacterial infections and mycoses, biology.organism_classification, medicine.disease, Fas receptor, Interleukin-12, Molecular biology, Interleukin-10, Interleukin 10, Infectious Diseases, Insect Science, Immunology, Leukocytes, Mononuclear, Cytokines, Parasitology, Borrelia Infections, Acrodermatitis chronica atrophicans
Abstract

Apoptosis of the lymphocytes plays an essential role in the regulation of inflammatory/immune responses and its abnormalities may contribute to a chronic infection, persistent inflammation and autoimmunity. Its role in the pathogenesis of the late Lyme borreliosis manifestations has not been studied so far. We have measured Th lymphocyte apoptosis rate, membrane expression of pro-apoptotic Fas receptor, and supernatant concentrations of selected soluble pro- and anti-apoptotic mediators in cultures of peripheral blood mononuclear cells from 16 patients with disseminated Lyme borreliosis (6 with osteoarticular symptoms, 7 with neuroborreliosis and 3 with acrodermatitis chronica atrophicans) and 8 healthy controls. The cultures stimulated for 48 h with live Borrelia burgdorferi sensu stricto, B. garinii or B. afzelii spirochetes. Fraction of the apoptotic Th (CD3+CD4+) lymphocytes and expression of Fas in this cell population was measured cytometrically and concentrations of soluble Fas, soluble Fas ligand, IL-10, IL-12 and TGF-β in culture supernatant with ELISA assays. The expression of IL-10, soluble and membrane Fas and soluble Fas ligand was increased under stimulation and higher in the presence of B. burgdorferi sensu stricto than the other species. Apoptosis rate was not affected. There was no difference between Lyme borreliosis patients and controls. IL-10 concentration correlated negatively with the membrane Fas expression and apoptosis under stimulation with B. afzelii and B. garinii . Expression of Fas/FasL system is up-regulated under stimulation with B. burgdorferi , but without corresponding increase in lymphocyte apoptosis. Variable responses observed with different B. burgdorferi species may reflect differences in the pathogenesis of the infection in vivo .

Published
2015
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50. Prognostic significance of ligands belonging to tumour necrosis factor superfamily in acute lymphoblastic leukaemia

Author

Janusz Dzięcioł, Marcin Moniuszko, U. Bolkun-Skornicka, Anna Szumowska, Dorota Lemancewicz, Ewa Jabłońska, Lukasz Bolkun, and Janusz Kloczko

Subjects
Adult, Male, Cancer Research, Programmed cell death, Neoplasm, Residual, Necrosis, Adolescent, Tumor Necrosis Factor Ligand Superfamily Member 13, Enzyme-Linked Immunosorbent Assay, TNF-Related Apoptosis-Inducing Ligand, Pathogenesis, Young Adult, hemic and lymphatic diseases, B-Cell Activating Factor, Biomarkers, Tumor, medicine, Humans, B-cell activating factor, Neoplasm Staging, Ligand, business.industry, SUPERFAMILY, Hematology, Middle Aged, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Prognosis, Survival Rate, Oncology, Apoptosis, Case-Control Studies, Immunology, Female, Tumor necrosis factor alpha, medicine.symptom, business, Follow-Up Studies
Abstract

Altered activities of ligands belonging to tumour necrosis factor (TNF) superfamily, namely B-cell activating factor (BAFF), a proliferation-inducing ligand (APRIL) and apoptosis inducing ligand (TRAIL) were demonstrated in several haematological diseases including acute lymphoblastic leukaemia (ALL). BAFF, APRIL and TRAIL provide crucial survival signals to immature, naive and activated B cells. These ligands are capable of activating a broad spectrum of intracellular signalling cascades that can either induce apoptosis or protect from programmed cell death. BAFF and APRIL, which can directly activate the NF-κB pathway, have been identified as crucial survival factors for ALL cells. Here, we have analyzed serum BAFF, APRIL and TRAIL concentrations in 48 patients with newly diagnosed ALL and 44 healthy volunteers. The levels of APRIL and BAFF were significantly higher in ALL patients as compared to healthy volunteers. In contrast, concentrations of TRAIL were significantly lower in ALL patients. Moreover, following induction, the levels of APRIL, but not BAFF or TRAIL, were significantly lower in a group of patients with complete remission (CR) as compared to non-respondent (NR) ALL patients. Furthermore, we demonstrated statistically significant differences in concentrations of APRIL between CR MRD-negative and CR, MRD-positive ALL patients. Notably detection of higher concentrations of APRIL was associated with shorter leukaemia-free survival and overall survival. Altogether, our data indicate that APRIL can play an important role in the pathogenesis of ALL and the measurement of APRIL levels can improve prognostication in ALL patients.

Published
2015
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