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Cervical cancer is one of the most common diseases in women and a role of human papilloma virus (HPV) as the trigger of malignization is considered to be proved. Virus replication influence alterations in gene expression pattern provoking proliferative and antiapoptotic activity of infected cell - fundamental features of malignization. We analyzed KI67, Aurora A, Birc5, cyclin B1, CTSL2, MMP11, Her2/neu, GRB7, BCL2, BAG1, BAX, NDRG1, ESR, PRG, PTEN, MGB and hTERT expression by qPCR in normal HPV positive/negative cervical epithelium (CE), through cervical intraepithelial neoplasia progression (CIN I-III) and in cervical cancer (CC). Materials and methods mRNA expression level of studied genes was quantified by qPCR in 76 tissue samples taken from women with CIN I (n = 17), CIN II (n = 11), CIN III (n = 12) and CC (n = 16). Negative control group included 20 samples of morphologically normal cervical epithelium (NE). Results Expression of KI67, CCNB1 and hTERT was increased during disease progression from normal epithelium through CIN to cervical cancer, but BCL2, BAG and BAX expression was simultaneously decreased (p = 0,0120). Expression of CCNB1 (p = 0,0042), BAG1 (p = 0,012) and hTERT (p = 0,0220) is much higher in CIN III than in CIN I-II. Besides gene expression alterations allow to classify normal, CIN and cancer tissues with 75-92% specificity (p Conclusion HPV persistence changes proliferative and antiapoptotic activity of NE, CIN and CC cells in different manner. Gene expression alterations in different degree of CIN and CC may be used as a classification model for determination of high risk neoplastic lesions in clinical practice.

Background: Long-term results of radiotherapy alone or in combined treatment with patients with locally advanced oral cancer are not very impressive with 5 year overall survival not higher than 56%. Photodynamic (PDT) therapy possesses its own anti-tumor activity and may sensitize to radioand chemotherapy. Purpose: To evaluate the potential significance of PDT in multimodality treatment of oral cancer. Materials and Methods: 35 patients (26 78 y.o.) with primary T2 4N0 1M0 and recurrent squamous cell oral cancer (tongue, superior/inferior lip, mouth basis) with contradictions to traditional radioor combined treatment were included in the current study. Photosensitizers “FOSCAN”, “FOTOLON”, “RADOCHLORIN” were used by intravenous injections, then patients were treated by light (semiconductor laser, wavelength 652 667 nm, output power 1W, light doses 50 300 J/cm2) in 3 96 h after injection. Adjuvant RT on neck lymph nodes begun on 10 14 days after PDT, in 3Gy/fraction 5 times a week, TD 30 36Gy (TDeq 40 44Gy) in N0 and 45 48Gy (TD eq 56 60Gy) in N1 patients. In 15 patients (42.9%) with primary tumour volume >3 cm3 or positive regional lymph nodes platinum-based neoadjuvant chemotherapy was used, with PDT after adequate tumour regression (tumour volume 0.5 2.5 cm3) or brachytherapy (60Co LDR, 192Ir HDR) in patients with larger tumours. Results: Objective response 30 patients (85.7%): complete response was achieved in 23 patients (65.7%), partial in 7 (20%). In 24 months period objective response was 26 patients (74.3%) patients, including cancer of lip 100%, tongue 70%, mouth basis 66%. No severe complications were recorder. Treatment had good cosmetic effect and high functional rehabilitation. Conclusion: PDT as a step of multimodality program expands the possibilities for radical treatment and improves long-term results in patients with oral cancer.