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1. The Alpine ibex (Capra ibex) gut microbiome, seasonal dynamics and potential application in lignocellulose bioconversion

Author

Nanetti, Enrico, primary, Scicchitano, Daniel, additional, Palladino, Giorgia, additional, Interino, Nicolò, additional, Corlatti, Luca, additional, Pedrotti, Luca, additional, Zanetti, Federica, additional, Pagani, Elena, additional, Esposito, Erika, additional, Brambilla, Alice, additional, Grignolio, Stefano, additional, Marotti, Ilaria, additional, Turroni, Silvia, additional, Fiori, Jessica, additional, Rampelli, Simone, additional, and Candela, Marco, additional

Published
2024
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4. Refinement of the NISECI ecological index reference conditions for Italian freshwater fish communities in the eastern Emilia-Romagna region

Author

Marchi, Andrea, primary, Bertaccini, Andrea, additional, Fan, Wenqu, additional, Zuffi, Gianluca, additional, Sacchetti, Stefano, additional, Nanetti, Matteo, additional, Lee, Chloe, additional, Agostini, Alessandra, additional, Lucchini, Daniela, additional, Bianconcini, Silvia, additional, Zaccanti, Francesco, additional, Goffredo, Stefano, additional, and Caroselli, Erik, additional

Published
2023
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5. Towards a free wild-caught fishmeal, fish oil and soy protein in European sea bass diet using by-products from fishery and aquaculture

Author

Marchi, Arianna, primary, Bonaldo, Alessio, additional, Di Biase, Andrea, additional, Cerri, Roberto, additional, Scicchitano, Daniel, additional, Nanetti, Enrico, additional, Candela, Marco, additional, Picone, Gianfranco, additional, Capozzi, Francesco, additional, Dondi, Francesco, additional, Gatta, Pier Paolo, additional, and Parma, Luca, additional

Published
2023
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6. Extreme phenotypic heterogeneity in non-expansion spinocerebellar ataxias

Author

Cunha, Paulina, primary, Petit, Emilien, additional, Coutelier, Marie, additional, Coarelli, Giulia, additional, Mariotti, Caterina, additional, Faber, Jennifer, additional, Van Gaalen, Judith, additional, Damasio, Joana, additional, Fleszar, Zofia, additional, Tosi, Michele, additional, Rocca, Clarissa, additional, De Michele, Giovanna, additional, Minnerop, Martina, additional, Ewenczyk, Claire, additional, Santorelli, Filippo M., additional, Heinzmann, Anna, additional, Bird, Thomas, additional, Amprosi, Matthias, additional, Indelicato, Elisabetta, additional, Benussi, Alberto, additional, Charles, Perrine, additional, Stendel, Claudia, additional, Romano, Silvia, additional, Scarlato, Marina, additional, Le Ber, Isabelle, additional, Bassi, Maria Teresa, additional, Serrano, Mercedes, additional, Schmitz-Hübsch, Tanja, additional, Doss, Sarah, additional, Van Velzen, Gijs A.J., additional, Thomas, Quentin, additional, Trabacca, Antonio, additional, Ortigoza-Escobar, Juan Dario, additional, D'Arrigo, Stefano, additional, Timmann, Dagmar, additional, Pantaleoni, Chiara, additional, Martinuzzi, Andrea, additional, Besse-Pinot, Elsa, additional, Marsili, Luca, additional, Cioffi, Ettore, additional, Nicita, Francesco, additional, Giorgetti, Alejandro, additional, Moroni, Isabella, additional, Romaniello, Romina, additional, Casali, Carlo, additional, Ponger, Penina, additional, Casari, Giorgio, additional, De Bot, Susanne T., additional, Ristori, Giovanni, additional, Blumkin, Lubov, additional, Borroni, Barbara, additional, Goizet, Cyril, additional, Marelli, Cecilia, additional, Boesch, Sylvia, additional, Anheim, Mathieu, additional, Filla, Alessandro, additional, Houlden, Henry, additional, Bertini, Enrico, additional, Klopstock, Thomas, additional, Synofzik, Matthis, additional, Riant, Florence, additional, Zanni, Ginevra, additional, Magri, Stefania, additional, Di Bella, Daniela, additional, Nanetti, Lorenzo, additional, Sequeiros, Jorge, additional, Oliveira, Jorge, additional, Van de Warrenburg, Bart, additional, Schöls, Ludger, additional, Taroni, Franco, additional, Brice, Alexis, additional, and Durr, Alexandra, additional

Published
2023
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8. Seasonal dynamics of the microbiome-host response to pharmaceuticals and pesticides in Mytilus galloprovincialis farmed in the Northwestern Adriatic Sea

Author

Palladino, Giorgia, primary, Rampelli, Simone, additional, Scicchitano, Daniel, additional, Nanetti, Enrico, additional, Iuffrida, Letizia, additional, Wathsala, Rajapaksha Haddokara Gedara Rasika, additional, Interino, Nicolò, additional, Marini, Mauro, additional, Porru, Emanuele, additional, Turroni, Silvia, additional, Fiori, Jessica, additional, Franzellitti, Silvia, additional, and Candela, Marco, additional

Published
2023
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9. Generation of an iPSC line from a patient with spastic paraplegia type 10 carrying a novel mutation in KIF5A gene

Author

Santangelo, Serena, primary, Bossolasco, Patrizia, additional, Magri, Stefania, additional, Colombrita, Claudia, additional, Invernizzi, Sabrina, additional, Gellera, Cinzia, additional, Nanetti, Lorenzo, additional, Di Bella, Daniela, additional, Silani, Vincenzo, additional, Taroni, Franco, additional, and Ratti, Antonia, additional

Published
2023
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10. Seasonal dynamics of the microbiome-host response to pharmaceuticals and pesticides in Mytilus galloprovincialis farmed in the Northwestern Adriatic Sea

Author

Giorgia Palladino, Simone Rampelli, Daniel Scicchitano, Enrico Nanetti, Letizia Iuffrida, Rajapaksha Haddokara Gedara Rasika Wathsala, Nicolò Interino, Mauro Marini, Emanuele Porru, Silvia Turroni, Jessica Fiori, Silvia Franzellitti, and Marco Candela

Subjects
Environmental Engineering, Environmental Chemistry, Pollution, Waste Management and Disposal
Published
2023
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12. Digenic inheritance of STUB1 variants and TBP polyglutamine expansions explains the incomplete penetrance of SCA17 and SCA48

Author

Magri, Stefania, primary, Nanetti, Lorenzo, additional, Gellera, Cinzia, additional, Sarto, Elisa, additional, Rizzo, Elena, additional, Mongelli, Alessia, additional, Ricci, Benedetta, additional, Fancellu, Roberto, additional, Sambati, Luisa, additional, Cortelli, Pietro, additional, Brusco, Alfredo, additional, Bruzzone, Maria Grazia, additional, Mariotti, Caterina, additional, Di Bella, Daniela, additional, and Taroni, Franco, additional

Published
2022
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14. Different patterns of movement-related cortical oscillations in patients with myoclonus and in patients with spinocerebellar ataxia

Author

Anna Castaldo, Alessia Mongelli, Laura Canafoglia, Caterina Mariotti, Elisa Visani, Silvana Franceschetti, Lorenzo Nanetti, and Ferruccio Panzica

Subjects
Adult, Male, Myoclonus, medicine.medical_specialty, Ataxia, Movement, Alpha (ethology), Motor Activity, 050105 experimental psychology, Lateralization of brain function, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Physiology (medical), Internal medicine, Reaction Time, medicine, Humans, Spinocerebellar Ataxias, 0501 psychology and cognitive sciences, In patient, Cortical Synchronization, Evoked Potentials, Cerebral Cortex, Cued speech, Electromyography, business.industry, 05 social sciences, Electroencephalography, Middle Aged, medicine.disease, Sensory Systems, medicine.anatomical_structure, Neurology, Spinocerebellar ataxia, Cardiology, Female, Neurology (clinical), medicine.symptom, business, 030217 neurology & neurosurgery, Motor cortex
Abstract

Objective To assess whether different patterns of EEG rhythms during a Go/No-go motor task characterize patients with cortical myoclonus (EPM1) or with spinocerebellar ataxia (SCA). Methods We analyzed event-related desynchronization (ERD) and synchronization (ERS) in the alpha and beta-bands during visually cued Go/No-go task in 22 patients (11 with EPM1, 11 with SCA) and 11 controls. Results In the Go condition, the only significant difference was a reduced contralateral beta-ERS in the EPM1 patients compared with controls; in the No-go condition, the EPM1 patients showed prolonged alpha-ERD in comparison with both controls and SCA patients, and reduced or delayed alpha- and beta-ERS in comparison with controls. In both conditions, the SCA patients, unlike EPM1 patients and controls, showed minimal or absent lateralization of alpha- and beta-ERD. Conclusions EPM1 patients showed abnormal ERD/ERS dynamics, whereas SCA patients mainly showed defective ERD lateralization. Significance A different behavior of ERS/ERD distinguished the two patient groups: the pattern observed in EPM1 suggests a prominent defect of inhibition occurring in motor cortex contralateral to activated segment, whereas the pattern observed in SCA suggested a defective lateralization attributable to the damage of cerebello-cortical network, which is instead marginal in patients with cortical myoclonus.

Published
2019
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15. Digenic inheritance of STUB1 variants and TBP polyglutamine expansions solves the enigma of SCA17 and SCA48 incomplete penetrance

Author

Franco Taroni, Elisa Sarto, Luisa Sambati, Caterina Mariotti, Daniela Di Bella, Pietro Cortelli, Cinzia Gellera, Maria Grazia Bruzzone, Elena Rizzo, Lorenzo Nanetti, Benedetta Ricci, Alfredo Brusco, Roberto Fancellu, Stefania Magri, and Alessia Mongelli

Subjects
Genetics, History, Ataxia, Polymers and Plastics, Biology, medicine.disease, Digenic inheritance, Penetrance, Industrial and Manufacturing Engineering, Missing heritability problem, medicine, Spinocerebellar ataxia, Business and International Management, Allele, medicine.symptom, Trinucleotide repeat expansion, Gene
Abstract

Purpose: To unravel the genetic factors underlying missing heritability in spinocerebellar ataxia type 17, caused by polyglutamine-encoding CAG/CAA repeat expansions in the TBP gene. Alleles with >49 CAG/CAA are fully penetrant. Most patients, however, carry intermediate TBP 41-49 alleles that show incomplete penetrance. Methods: Using NGS approaches, we investigated 40 SCA17/TBPM41-54 index patients, their affected (n=55) and unaffected (n=51) relatives, and a cohort of ataxia patients (n=292). Results: All but one (30/31) index cases with TBP 41-46 alleles carried a heterozygous pathogenic variant in the STUB1 gene associated with spinocerebellar ataxias SCAR16 (recessive) and SCA48 (dominant). No STUB1 variant was found in patients carrying TBP 47-54 alleles. TBP expansions and STUB1 variants cosegregate in all affected family members, while the presence of either one alone was never associated with disease. Conclusions: Our data reveal an unexpected interaction between STUB1 and TBP in the pathogenesis of SCA17 and raise questions on the existence of SCA48 as a monogenic disease, with crucial implications for diagnosis and counselling. They provide a conclusive explanation for the incomplete penetrance of intermediate TBP alleles and demonstrate a dual inheritance pattern for SCA17, which is a monogenic dominant disorder for TBP ≥47 alleles and a digenic TBP/STUB1 disease (SCA17-DI) for intermediate expansions.

Published
2021
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16. Progression characteristics of the European Friedreich's Ataxia Consortium for Translational Studies (EFACTS): a 4-year cohort study

Author

Reetz, Kathrin, primary, Dogan, Imis, additional, Hilgers, Ralf-Dieter, additional, Giunti, Paola, additional, Parkinson, Michael H, additional, Mariotti, Caterina, additional, Nanetti, Lorenzo, additional, Durr, Alexandra, additional, Ewenczyk, Claire, additional, Boesch, Sylvia, additional, Nachbauer, Wolfgang, additional, Klopstock, Thomas, additional, Stendel, Claudia, additional, Rodríguez de Rivera Garrido, Francisco Javier, additional, Rummey, Christian, additional, Schöls, Ludger, additional, Hayer, Stefanie N, additional, Klockgether, Thomas, additional, Giordano, Ilaria, additional, Didszun, Claire, additional, Rai, Myriam, additional, Pandolfo, Massimo, additional, Schulz, Jörg B, additional, Labrum, Robyn, additional, Thomas-Black, Gilbert, additional, Manso, Katarina, additional, Solanky, Nita, additional, Gellera, Cinzia, additional, Mongelli, Alessia, additional, Castaldo, Anna, additional, Fichera, Mario, additional, Palau, Francesc, additional, O'Callaghan, Mar, additional, Biet, Marie, additional, Monin, Marie Lorraine, additional, Eigentler, Andreas, additional, Indelicato, Elisabetta, additional, Amprosi, Matthias, additional, Radelfahr, Florentine, additional, Bischoff, Almut T., additional, Holtbernd, Florian, additional, Brcina, Nikolina, additional, Hohenfeld, Christian, additional, Koutsis, Georgios, additional, Breza, Marianthi, additional, Bertini, Enrico, additional, and Vasco, Gessica, additional

Published
2021
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17. Multiple system atrophy and CAG repeat length: A genetic screening of polyglutamine disease genes in Italian patients

Author

Alessia Mongelli, Nicoletta Meucci, Gianni Pezzoli, Elena Rizzo, Caterina Mariotti, Lidia Sarro, Franco Taroni, Stefano Goldwurm, Lorenzo Nanetti, and Cinzia Gellera

Subjects
Male, 0301 basic medicine, Genotype, Disease, 030105 genetics & heredity, 03 medical and health sciences, 0302 clinical medicine, Atrophy, Gene Frequency, stomatognathic system, parasitic diseases, mental disorders, medicine, Humans, Genetic Predisposition to Disease, Genetic Testing, Allele, Ataxin-1, Aged, Ataxin-2, Genetic testing, Cerebellar ataxia, medicine.diagnostic_test, business.industry, General Neuroscience, Parkinsonism, Middle Aged, Multiple System Atrophy, medicine.disease, nervous system diseases, Italy, nervous system, Immunology, Etiology, Spinocerebellar ataxia, Female, medicine.symptom, Peptides, Trinucleotide Repeat Expansion, business, 030217 neurology & neurosurgery
Abstract

Multiple system atrophy (MSA) is an adult onset, progressive, neurodegenerative disorder of unknown etiology characterized by autonomic dysfunction, parkinsonism (MSA-P) and cerebellar ataxia (MSA-C). The phenotypic spectrum may present overlapping features with other neurodegenerative diseases, particularly the autosomal dominant inherited polyglutamine disorders. To investigate the possible contribution of CAG expansions in the MSA phenotype, we analyzed the triplet repeat length in the autosomal dominant causative genes for spinocerebellar ataxia (SCA) type 1, 2, 3, 6, 7, 17, dentatorubral-pallidoluysian atrophy (DRPLA) and Huntington disease (HD) in a cohort of 246 Italian MSA patients. As comparison, 223 controls were also analyzed. The alleles were classified on the basis of CAG repeat length as "normal", "intermediate" or "expanded" according to literature. The MSA patients (101 men/145 women) had a mean age at onset of 58 years and a mean age at genetic testing of 63 years. MSA-C patients had significantly younger age at onset and at examination in comparison to MSA-P (p 0.0001). We identified a SCA1 intermediate allele in a MSA-C subject (36 CAG), a SCA2 intermediate allele in a MSA-P patient (31 CAG), and a pathologically expanded SCA2 allele (36 CAG) in a patient initially misdiagnosed as MSA-C. No intermediate or expanded SCA alleles were detected in controls. The distribution of CAG repeat length was similar among groups except for SCA1 gene that showed a higher percentage of longer normal alleles in MSA-C as compared to MSA-P and controls (p 0.0001). This study supports the utility of polyQ genetic testing in the differential diagnosis of MSA, and may suggest a possible role of SCA1 repeat length as risk factor for MSA-C. SCA1 and SCA2 genetic screening is recommended in MSA Italian patients.

Published
2018
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18. Cortical thickness, stance control, and arithmetic skill: An exploratory study in premanifest Huntington disease

Author

Caterina Mariotti, Valeria Elisa Contarino, Marina Grisoli, Anne-Catherine Bachoud-Lévi, Maria Grazia Bruzzone, Elena Rizzo, Lidia Sarro, Franco Taroni, Maria Giavazzi, Serena Frittoli, Anna Castaldo, Cinzia Gellera, Lorenzo Nanetti, and Andrea Ciammola

Subjects
Adult, Male, 0301 basic medicine, Heterozygote, Prodromal Symptoms, Disease, Motor symptoms, Upper Extremity, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Basal ganglia, Humans, Medicine, Cognitive Dysfunction, Arithmetic, Postural Balance, Cerebral Cortex, business.industry, Posturography, Neurodegeneration, Symbol digit modalities test, Cognition, Mathematical Concepts, Middle Aged, medicine.disease, Magnetic Resonance Imaging, Corpus Striatum, Huntington Disease, 030104 developmental biology, Neurology, Valid Biomarker, Female, Neurology (clinical), Geriatrics and Gerontology, business, Psychomotor Performance, 030217 neurology & neurosurgery
Abstract

Huntington disease (HD) is an inherited neurodegenerative disorder most commonly manifesting in adulthood. Identification of biomarkers tracking neurodegeneration before the onset of motor symptoms is important for future interventional studies. Our study aimed to contribute in the phenotypic characterization of the premanifest HD phase.28 premanifest subjects (preHD), 25 age-matched controls, and 12 manifest HD patients were enrolled for the study. The participants underwent a multimodal protocol including cognitive evaluations, arithmetic ability test, posturography, composite cerebellar functional test (CCFS), and brain 3T-MRI. PreHD were divided at the group median for predicted years to expected onset into "far-from-onset" (15 years, PreHD-far), and "close-to-onset" (≤15 years, preHD-close). Basal ganglia volumes and cortical thickness were computed using FreeSurfer.PreHD-close showed significantly lower scores than controls in Symbol Digit Modalities Test (p = 0.017), Arithmetic subtraction task (p = 0.04), and MMSE (p 0.006). At posturography, preHD-close showed increased sway velocity (0.04) and distance (p 0.02) compared to controls. PreHD-close had reduced striatum and globus pallidus volumes and left occipital cortical thinning compared to controls. Compared to PreHD far-from-onset, PreHD-close showed bilateral cortical thinning in occipital and parahippocampal regions, inversely correlating with burden score and prognostic index for HD. CCFS only differed between controls and manifest HD. PreHD far-from-onset did not show significant differences in comparison with controls.We confirmed that quantitative brain MRI represents a valid biomarker of neurodegeneration in preHD. Posturography and Arithmentic tests seem promising tools for detecting early changes in premanifest HD, but need to be further confirmed in large cohorts.

Published
2018
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19. Digenic inheritance of STUB1 variants and TBP polyglutamine expansions solves the enigma of SCA17 and SCA48 incomplete penetrance

Author

Magri, Stefania , primary, Nanetti, Lorenzo, additional, Gellera, Cinzia, additional, Sarto, Elisa, additional, Rizzo, Elena , additional, Mongelli , Alessia, additional, Ricci, Benedetta  , additional, Fancellu , Roberto, additional, Sambati , Luisa, additional, Cortelli , Pietro, additional, Brusco, Alfredo, additional, Grazia Bruzzone , Maria, additional, Mariotti, Caterina, additional, Di Bella, Daniela, additional, and Taroni, Franco, additional

Published
2021
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21. Conversion of individuals at risk for spinocerebellar ataxia types 1, 2, 3, and 6 to manifest ataxia (RISCA): a longitudinal cohort study

Author

Jacobi, Heike, primary, du Montcel, Sophie Tezenas, additional, Romanzetti, Sandro, additional, Harmuth, Florian, additional, Mariotti, Caterina, additional, Nanetti, Lorenzo, additional, Rakowicz, Maria, additional, Makowicz, Grzegorz, additional, Durr, Alexandra, additional, Monin, Marie-Lorraine, additional, Filla, Alessandro, additional, Roca, Alessandro, additional, Schöls, Ludger, additional, Hengel, Holger, additional, Infante, Jon, additional, Kang, Jun-Suk, additional, Timmann, Dagmar, additional, Casali, Carlo, additional, Masciullo, Marcella, additional, Baliko, Laszlo, additional, Melegh, Bela, additional, Nachbauer, Wolfgang, additional, Bürk-Gergs, Katrin, additional, Schulz, Jörg B, additional, Riess, Olaf, additional, Reetz, Kathrin, additional, and Klockgether, Thomas, additional

Published
2020
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22. Progression Characteristics in Friedreich's Ataxia: A 4-Year Observational Study

Author

Reetz, Kathrin, primary, Dogan, Imis, additional, Hilgers, Ralf-Dieter, additional, Giunti, Paola, additional, Mariotti, Caterina, additional, Nanetti, Lorenzo, additional, Durr, Alexandra, additional, Ewenczyk, Claire, additional, Boesch, Sylvia, additional, Nachbauer, Wolfgang, additional, Klopstock, Thomas, additional, Stendel, Claudia, additional, Javier Rodríguez de Rivera Garrido, Francisco, additional, Schöls, Ludger, additional, Hayer, Stefanie, additional, Klockgether, Thomas, additional, Giordano, Ilaria, additional, Didszun, Claire, additional, Rai, Myriam, additional, Pandolfo, Massimo, additional, Schulz, Jörg B., additional, and Group, EFACTS Study, additional

Published
2020
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23. Effect of 1-y oral supplementation with vitaminized olive oil on platelets from healthy postmenopausal women

Author

Eleonora Salvolini, Jacopo Sabbatinelli, Laura Mazzanti, Laura Nanetti, Arianna Vignini, Nelvio Cester, Francesca Raffaelli, and Veronica Quagliarini

Subjects
Blood Platelets, medicine.medical_specialty, Antioxidant, Mediterranean diet, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Osteoporosis, 030209 endocrinology & metabolism, 030204 cardiovascular system & hematology, Nitric Oxide, Placebo, Nitric oxide, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Peroxynitrous Acid, Internal medicine, medicine, Membrane fluidity, Humans, Olive Oil, Cholecalciferol, Nutrition and Dietetics, business.industry, Vitamin K 1, Vitamins, Middle Aged, medicine.disease, Vitamin B 6, Postmenopause, Menopause, Oxidative Stress, Endocrinology, chemistry, Nitrosative Stress, Dietary Supplements, Female, business, Peroxynitrite
Abstract

Objective Olive oil is the main fat source in the Mediterranean diet and shows a protective role against aging and related diseases. Osteoporosis represents a serious health problem worldwide and is associated with an increased risk for fractures and mortality. Nutrition should be part of bone disease prevention strategies, especially in light of the aging population and the effect of diet on bone health. The aim of this study was to investigate whether oral supplementation with extra virgin olive oil (VOO) enriched with vitamins D3, K1, and B6 (VitVOO) is able to modify some physicochemical and functional plasma membrane properties and nitrosative stress markers status. Methods In this single-center, randomized placebo-controlled trial, 60 postmenopausal women were administered either VitVOO or placebo (PlaVOO). After 1 y of oral supplementation, platelet membrane fluidity changes, Na+/K+-ATPase activity, serum nitric oxide, and peroxynitrite levels were determined in participants. Results After 1 y (time 1), women taking VitVOO showed lower nitric oxide levels than those taking PlaVOO; the same trend was found for peroxynitrite levels. As far as membrane fluidity was concerned, a significant decrease in anisotropy of diphenylhexatriene and trimethylammonium-diphenylhexatriene at time 1 in VitVOO participants compared with PlaVOO was found. Finally, Na+/K+-ATPase activity showed a significant increase after VitVOO supplementation. Conclusion The supplementation of VitVOO into the diet of postmenopausal women could represent a proper tool for platelet function and a useful strategy against nitrosative stress and related diseases, thus confirming the antioxidant role played by the added vitamins.

Published
2017
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24. Long-term disease progression in spinocerebellar ataxia types 1, 2, 3, and 6: a longitudinal cohort study

Author

Massimo Pandolfo, Sylvia Boesch, Laszlo Baliko, Thomas Klockgether, Sandra Szymanski, Ludger Schöls, Alessandro Filla, Anna Sulek, Arron Cook, Bart P.C. van de Warrenburg, Heike Jacobi, Maria Rakowicz, Jun Suk Kang, Holger Hengel, Michael H Parkinson, Dagmar Timmann, Anna Sobanska, Jon Infante, Alexis Brice, Peter Bauer, Jörg B. Schulz, Lorenzo Nanetti, Sophie Tezenas du Montcel, Antonella Antenora, Cecilia Marelli, Alexandra Durr, Paola Giunti, Robyn Labrum, Marta Panzeri, Béla Melegh, Alhassane Diallo, Perrine Charles, Caterina Mariotti, Sonia Molho, José Berciano, Tanja Schmitz-Hübsch, Jacobi, Heike, du Montcel, Sophie Tezena, Bauer, Peter, Giunti, Paola, Cook, Arron, Labrum, Robyn, Parkinson, Michael H, Durr, Alexandra, Brice, Alexi, Charles, Perrine, Marelli, Cecilia, Mariotti, Caterina, Nanetti, Lorenzo, Panzeri, Marta, Rakowicz, Maria, Sulek, Anna, Sobanska, Anna, Schmitz Hübsch, Tanja, Schöls, Ludger, Hengel, Holger, Baliko, Laszlo, Melegh, Bela, Filla, Alessandro, Antenora, Antonella, Infante, Jon, Berciano, José, van de Warrenburg, Bart P, Timmann, Dagmar, Szymanski, Sandra, Boesch, Sylvia, Kang, Jun Suk, Pandolfo, Massimo, Schulz, Jörg B, Molho, Sonia, Diallo, Alhassane, and Klockgether, Thomas

Subjects
Male, Pediatrics, Medizin, physiopathology [Spinocerebellar Ataxias], Cohort Studies, genetics [Ataxin-1], ATXN2 protein, human, genetics [Spinocerebellar Ataxias], Young adult, Ataxin-3, Ataxin-1, Ataxin-2, CACNA1A protein, human, medicine.diagnostic_test, Middle Aged, Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3], classification [Spinocerebellar Ataxias], Natural history, genetics [Ataxin-2], genetics [Calcium Channels], Disease Progression, Spinocerebellar ataxia, Female, medicine.symptom, Cohort study, Adult, PubMed, congenital, hereditary, and neonatal diseases and abnormalities, medicine.medical_specialty, Ataxia, Referral, Young Adult, genetics [Ataxin-3], statistics & numerical data [PubMed], medicine, Spinocerebellar Ataxias, Humans, ATXN1 protein, human, ddc:610, Genetic testing, business.industry, ATXN3 protein, human, medicine.disease, Repressor Proteins, genetics [Repressor Proteins], Sample size determination, Physical therapy, Calcium Channels, Neurology (clinical), business
Abstract

Item does not contain fulltext BACKGROUND: Spinocerebellar ataxias are dominantly inherited neurodegenerative diseases. As potential treatments for these diseases are being developed, precise knowledge of their natural history is needed. We aimed to study the long-term disease progression of the most common spinocerebellar ataxias: SCA1, SCA2, SCA3, and SCA6. Furthermore, we aimed to establish the order and occurrence of non-ataxia symptoms, and identify predictors of disease progression. METHODS: In this longitudinal cohort study (EUROSCA), we enrolled men and women with positive genetic testing for SCA1, SCA2, SCA3, or SCA6 and with progressive, otherwise unexplained ataxia who were aged 18 years or older from 17 ataxia referral centres in ten European countries. Patients were seen every year for 3 years, and at irregular intervals thereafter. The primary outcome was the scale for the assessment and rating of ataxia (SARA), and the inventory of non-ataxia signs (INAS). We used linear mixed models to analyse progression. To account for dropouts, we applied a pattern-mixture model. This study is registered with ClinicalTrials.gov, number NCT02440763. FINDINGS: Between July 1, 2005, and Aug 31, 2006, 526 patients with SCA1, SCA2, SCA3, or SCA6 were enrolled. We analysed data for 462 patients with at least one follow-up visit. Median observation time was 49 months (IQR 35-72). SARA progression data were best fitted with a linear model in all genotypes. Annual SARA score increase was 2.11 (SE 0.12) in patients with SCA1, 1.49 (0.07) in patients with SCA2, 1.56 (0.08) in patients with SCA3, and 0.80 (0.09) in patients with SCA6. The increase of the number of non-ataxia signs reached a plateau in SCA1, SCA2, and SCA3. In patients with SCA6, the number of non-ataxia symptoms increased linearly, but more slowly than in patients with SCA1, SCA2, and SCA3 (p

Published
2015
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28. Age-related changes on platelet membrane: A study on elderly and centenarian monozygotic twins

Author

Laura, Nanetti, Nanetti, Laura, Cinzia, Moroni, Moroni, Cinzia, Arianna, Vignini, Vignini, Arianna, Patrizia, Vannini, Vannini, Patrizia, Claudio, Franceschi, Franceschi, Claudio, Laura, Mazzanti, Mazzanti, Laura, Nanetti L., Moroni C., Vignini A., Vannini P., Franceschi C., and Mazzanti L.

Subjects
Adult, Blood Platelets, Lipid Peroxides, Aging, medicine.medical_specialty, Membrane Fluidity, media_common.quotation_subject, Longevity, Monozygotic twin, Fluorescence Polarization, Biology, Biochemistry, Basal (phylogenetics), Endocrinology, Malondialdehyde, Internal medicine, Genetics, Membrane fluidity, medicine, Humans, Molecular Biology, Cellular Senescence, Aged, media_common, Aged, 80 and over, Successful aging, Lipid peroxide, Cell Membrane, Twins, Monozygotic, Cell Biology, Middle Aged, N-Acetylneuraminic Acid, Sodium-Potassium-Exchanging ATPase, Centenarian, Cell aging
Abstract

Cellular senescence is a biological process associated with aging and longevity. Successful aging is believed to be related to the ability to cope with different environmental stresses. The objective of this study was to investigate if cellular senescence is associated with platelet membrane modifications on subjects of different age, in particular on monozygotic twins and if these changes might be affected by both genetic components and environmental factors. The work was performed on 81 monozygotic twin pairs of different age. Platelet membranes from centenarian twins showed: decreased both basal lipid peroxide levels and membrane fluidity compared with elderly subjects; Na(+)/K(+)-ATPase activity and SA content are similar to those evaluated in young group, suggesting one of their important roles in the successful aging. We concluded that platelet membranes from centenarians show deeper structural and functional modifications than in elderly subjects and that these changes might play a protective role against oxidative damage. No statistical difference in biochemical parameters was observed between two sibpairs in each twin pair highlighting that environmental factors (diet, life-style) affect age-related platelet membrane changes less than their common genetic component. Thus genetic factors might play an important role in the mechanisms at the basis of successful aging.

Published
2005
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29. Biological and clinical characteristics of the European Friedreich's Ataxia Consortium for Translational Studies (EFACTS) cohort: a cross-sectional analysis of baseline data

Author

Kathrin Reetz, Wolfgang Nachbauer, Ana Sofia Costa, Thomas Klopstock, Marta Panzeri, Alexandra Durr, Michael H Parkinson, Katrin Bürk, Javier Arpa, Jörg B. Schulz, Lorenzo Nanetti, Kathrin Fedosov, Manuel Dafotakis, Chantal Depondt, Imis Dogan, Thomas Klockgether, Massimo Pandolfo, Sylvia Boesch, Perrine Charles, Ilaria Giordano, Ludger Schöls, Jennifer Müller vom Hagen, Ivan Karin, Paola Giunti, Mary G. Sweeney, Irene Sanz-Gallego, and Caterina Mariotti

Subjects
Adult, Male, congenital, hereditary, and neonatal diseases and abnormalities, Pediatrics, medicine.medical_specialty, methods [Translational Research, Biomedical], Ataxia, Adolescent, Databases, Factual, Late onset, Cohort Studies, Translational Research, Biomedical, Young Adult, Trinucleotide Repeats, diagnosis [Friedreich Ataxia], Activities of Daily Living, medicine, Humans, ddc:610, methods [Translational Medical Research], Longitudinal Studies, Prospective Studies, Child, Prospective cohort study, Aged, biology, epidemiology [Friedreich Ataxia], epidemiology [Europe], Middle Aged, medicine.disease, genetics [Friedreich Ataxia], Europe, Cross-Sectional Studies, Friedreich Ataxia, genetics [Trinucleotide Repeats], Cohort, Spinocerebellar ataxia, Physical therapy, Frataxin, biology.protein, Female, Neurology (clinical), Age of onset, medicine.symptom, Psychology, Cohort study
Abstract

Summary Background Friedreich's ataxia is a rare autosomal recessive neurodegenerative disorder. Here we report cross-sectional baseline data to establish the biological and clinical characteristics for a prospective, international, European Friedreich's ataxia database registry. Methods Within the European Friedreich's Ataxia Consortium for Translational Studies (EFACTS) framework, we assessed a cohort of patients with genetically confirmed Friedreich's ataxia. The primary outcome measure was the Scale for the Assessment and Rating of Ataxia (SARA) and secondary outcome measures were the Inventory of Non-Ataxia Signs (INAS), the performance-based coordination test Spinocerebellar Ataxia Functional Index (SCAFI), the neurocognitive phonemic verbal fluency test, and two quality-of-life measures: the activities of daily living (ADL) part of the Friedreich's Ataxia Rating Scale and EQ-5D. The Friedreich's ataxia cohort was subdivided into three groups: early disease onset (≤14 years), intermediate onset (15–24 years), and late onset (≥25 years), which were compared for clinical characteristics and outcome measures. We used linear regression analysis to estimate the annual decline of clinical outcome measures based on disease duration. This study is registered with ClinicalTrials.gov, number NCT02069509. Findings We enrolled 592 patients with genetically confirmed Friedreich's ataxia between Sept 15, 2010, and April 30, 2013, at 11 sites in seven European countries. Age of disease onset was inversely correlated with the number of GAA repeats in the frataxin ( FXN ) gene: every 100 GAA repeats on the smaller repeat allele was associated with a 2·3 year (SE 0·2) earlier onset. Regression analyses showed significant estimated annual worsening of SARA (regression coefficient 0·86 points [SE 0·05], INAS (0·14 points [0·01]), SCAFI Z scores (−0·09 [0·01]), verbal fluency (−0·34 words [0·07]), and ADL (0·64 points [0·04]) during the first 25 years of disease; the regression slope for health-related quality-of-life state from EQ-5D was not significant (−0·33 points [0·18]). For SARA, the predicted annual rate of worsening was significantly higher in early-onset patients (n=354; 1·04 points [0·13]) and intermediate-onset patients (n=137; 1·17 points [0·22]) than in late-onset patients (n=100; 0·56 points [0·10]). Interpretation The results of this cross-sectional baseline analysis of the EFACTS cohort suggest that earlier disease onset is associated with larger numbers of GAA repeats and more rapid disease progression. The differential estimated progression of ataxia symptoms related to age of onset have implications for the design of clinical trials in Friedreich's ataxia, for which SARA might be the most suitable measure to monitor disease progression. Funding European Commission.

Published
2015
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30. Event related desynchronization and synchronization reveals different dysfunction in SCA with respect to EPM1 patients

Author

Alessia Mongelli, Ferruccio Panzica, Anna Castaldo, S. Franceschetti, D. Rossi Sebastiano, Lorenzo Nanetti, Caterina Mariotti, Laura Canafoglia, and Elisa Visani

Subjects
medicine.medical_specialty, medicine.diagnostic_test, business.industry, Event related desynchronization, Alpha (ethology), Electroencephalography, medicine.disease, Sensory Systems, Lateralization of brain function, Beta band, Neurology, Physiology (medical), Internal medicine, Spinocerebellar ataxia, medicine, Cardiology, Neurology (clinical), Latency (engineering), business, Beta (finance)
Abstract

We evaluated patients with spinocerebellar ataxia (SCA) and Unverricht-Lundborg disease (EPM1) to compare motor related EEG modulation associated with selective cerebellar dysfunction or action myoclonus. We analyzed high-resolution EEG desynchronization/synchronization (ERD/ERS) in alpha and beta bands, occurring in response to a ‘Go’and ‘no-Go’ motor tasks in 11 SCA, in 11 EPM1 patients and in 10 healthy controls. In ‘Go’ condition, alpha-ERD showed a similar time course in patients and controls, while in ‘no-Go’ condition SCA and EPM1 patients showed a significantly protracted ERD duration, compared to controls. The latency to reach maximal alpha ERD peak was significantly shortened in ‘no-Go’ with respect to ‘GO’ condition in controls and SCA patients, but not in EPM1. Only in SCA patients, the area under the ERD was similar on both hemispheres. In the beta band, ERS maximal amplitude was lower and the time to reach its maximal value was reduced only in EPM1 patients. Only in SCA patients the area under ERD was similar on both hemispheres. Both SCA and EPM1 patients showed a disordered ERD/ERS pattern, suggesting a defective ability of EPM1 in no-Go and post movement pattern, and a mainly defective lateralization in SCA patients.

Published
2019
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31. Cortical thickness, stance control, and arithmetic skill: An exploratory study in premanifest Huntington disease

Author

Nanetti, Lorenzo, primary, Contarino, Valeria Elisa, additional, Castaldo, Anna, additional, Sarro, Lidia, additional, Bachoud-Levi, Anne-Catherine, additional, Giavazzi, Maria, additional, Frittoli, Serena, additional, Ciammola, Andrea, additional, Rizzo, Elena, additional, Gellera, Cinzia, additional, Bruzzone, Maria Grazia, additional, Taroni, Franco, additional, Grisoli, Marina, additional, and Mariotti, Caterina, additional

Published
2018
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33. Survival in patients with spinocerebellar ataxia types 1, 2, 3, and 6 (EUROSCA): a longitudinal cohort study

Author

Diallo, Alhassane, primary, Jacobi, Heike, additional, Cook, Arron, additional, Labrum, Robyn, additional, Durr, Alexandra, additional, Brice, Alexis, additional, Charles, Perrine, additional, Marelli, Cecilia, additional, Mariotti, Caterina, additional, Nanetti, Lorenzo, additional, Panzeri, Marta, additional, Rakowicz, Maria, additional, Sobanska, Anna, additional, Sulek, Anna, additional, Schmitz-Hübsch, Tanja, additional, Schöls, Ludger, additional, Hengel, Holger, additional, Melegh, Bela, additional, Filla, Alessandro, additional, Antenora, Antonella, additional, Infante, Jon, additional, Berciano, José, additional, van de Warrenburg, Bart P, additional, Timmann, Dagmar, additional, Boesch, Sylvia, additional, Pandolfo, Massimo, additional, Schulz, Jörg B, additional, Bauer, Peter, additional, Giunti, Paola, additional, Kang, Jun-Suk, additional, Klockgether, Thomas, additional, and Tezenas du Montcel, Sophie, additional

Published
2018
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35. Biological effects of palytoxin-like compounds from Ostreopsis cf. ovata: A multibiomarkers approach with mussels Mytilus galloprovincialis

Author

Stefania Gorbi, Francesca Raffaelli, A. Binelli, Arianna Vignini, Roberta Orletti, Laura Nanetti, Simone Bacchiocchi, Cecilia Totti, Stefano Accoroni, Francesco Regoli, and Raffaella Bocchetti

Subjects
Risk, Environmental Engineering, Harmful Algal Bloom, Health, Toxicology and Mutagenesis, Biology, Ecotoxicology, medicine.disease_cause, Algal bloom, Lipid peroxidation, Mice, chemistry.chemical_compound, Cnidarian Venoms, Palytoxin, Botany, medicine, Animals, Environmental Chemistry, Mytilus, chemistry.chemical_classification, Acrylamides, Analysis of Variance, Principal Component Analysis, Reactive oxygen species, fungi, Public Health, Environmental and Occupational Health, Free Radical Scavengers, General Medicine, General Chemistry, Mussel, Malondialdehyde, biology.organism_classification, Pollution, chemistry, Biochemistry, Dinoflagellida, Biological Assay, Biomarkers, Oxidative stress
Abstract

Massive blooms of the harmful benthic dinoflagellate Ostreopsis cf. ovata are of growing environmental concern in the Mediterranean, having recently caused adverse effects on benthic invertebrates and also some intoxication episodes to humans. The toxicological potential of produced palytoxin-like compounds was investigated in the present study on a typical marine sentinel species, the mussel Mytilus galloprovincialis . Organisms were sampled during various phases of a O . cf. ovata bloom, in two differently impacted sites. The presence of the algal toxins was indirectly assessed in mussels tissues (mouse test and hemolysis neutralization assay), while biological and toxicological effects were evaluated through the measurement of osmoregulatory and neurotoxic alterations (Na + /K + -ATPase and acetylcholinesterase activities), oxidative stress responses (antioxidant defences and total oxyradical scavenging capacity), lipid peroxidation processes (level of malondialdehyde), peroxisomal proliferation, organelle dysfunctions (lysosomal membrane stability, accumulation of lipofuscin and neutral lipids), immunological impairment (granulocytes percentage). Obtained results demonstrated a significant accumulation of algal toxins in mussels exposed to O . cf. ovata. These organisms exhibited a marked inhibition of the Na + /K + -ATPase activity and alterations of immunological, lysosomal and neurotoxic responses. Markers of oxidative stress showed more limited variations suggesting that toxicity of the O. cf. ovata toxins is not primarily mediated by an over production of reactive oxygen species. This study provided preliminary results on the usefulness of a multi-biomarker approach to assess biological alterations and toxicological events associated to blooms of O. cf. ovata in marine organisms.

Published
2012
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36. Nitric oxide platelet production in spontaneous miscarriage in the first trimester

Author

Paola Vitali, Stefano Raffaele Giannubilo, Laura Nanetti, Laura Mazzanti, Andrea L. Tranquilli, Arianna Vignini, Claudia Maria Curzi, Angelo Turi, and Francesca Raffaelli

Subjects
Adult, Blood Platelets, medicine.medical_specialty, Nitric Oxide Synthase Type III, Nitric Oxide Synthase Type II, Abortion, Nitric Oxide, Nitric oxide, Miscarriage, chemistry.chemical_compound, Pregnancy, Peroxynitrous Acid, medicine, Humans, Retrospective Studies, biology, Obstetrics, business.industry, Nitrotyrosine, Obstetrics and Gynecology, Gestational age, medicine.disease, Abortion, Spontaneous, Nitric oxide synthase, Pregnancy Trimester, First, Reproductive Medicine, chemistry, Case-Control Studies, biology.protein, Tyrosine, Gestation, Female, business
Abstract

Objective To investigate the role played by platelet nitric oxide (NO) metabolism in patients with spontaneous miscarriage (SM) and recurrent spontaneous miscarriage (RSM) compared with healthy pregnant women. Design Retrospective case-control study. Setting Patients and controls in an academic research environment. Intervention(s) None. Patient(s) Thirty singleton pregnant women who experienced SM, nine singleton pregnant women who presented with RSM, and 30 singleton healthy pregnant women matched for age, parity, and gestational age were enrolled. Main Outcome Measure(s) NO levels and peroxynitrite (ONOO - ) production; moreover, inducible NO synthase (iNOS), endothelial NO synthase (eNOS), and nitrotyrosine expression (N-Tyr) were observed in the same samples. Result(s) A significant increase was shown in platelet NO and ONOO - levels and in iNOS and N-Tyr both in SM and in RSM pregnant women compared with controls. Conclusion(s) The data herein reported imply that a modified NO pathway might play a key role in the physiological changes of advancing gestation but may also contribute to the pathophysiology of spontaneous miscarriage. Thus, any factors balancing NO metabolism might be useful in the treatment of miscarriage, thus reducing the substantial morbidity and associated mortality.

Published
2010
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38. Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

Author

Moss, Davina J Hensman, primary, Pardiñas, Antonio F, additional, Langbehn, Douglas, additional, Lo, Kitty, additional, Leavitt, Blair R, additional, Roos, Raymund, additional, Durr, Alexandra, additional, Mead, Simon, additional, Holmans, Peter, additional, Jones, Lesley, additional, Tabrizi, Sarah J, additional, Coleman, A, additional, Santos, R Dar, additional, Decolongon, J, additional, Sturrock, A, additional, Bardinet, E, additional, Ret, C Jauff, additional, Justo, D, additional, Lehericy, S, additional, Marelli, C, additional, Nigaud, K, additional, Valabrègue, R, additional, van den Bogaard, SJA, additional, Dumas, E M, additional, van der Grond, J, additional, t'Hart, EP, additional, Jurgens, C, additional, Witjes-Ane, M-N, additional, Arran, N, additional, Callaghan, J, additional, Stopford, C, additional, Frost, C, additional, Jones, R, additional, Hobbs, N, additional, Lahiri, N, additional, Ordidge, R, additional, Owen, G, additional, Pepple, T, additional, Read, J, additional, Say, M, additional, Wild, E, additional, Patel, A, additional, Fox, N C, additional, Gibbard, C, additional, Malone, I, additional, Crawford, H, additional, Whitehead, D, additional, Keenan, S, additional, Cash, D M, additional, Berna, C, additional, Bechtel, N, additional, Bohlen, S, additional, Man, A Hoff, additional, Kraus, P, additional, Axelson, E, additional, Wang, C, additional, Acharya, T, additional, Lee, S, additional, Monaco, W, additional, Campbell, C, additional, Queller, S, additional, Whitlock, K, additional, Campbell, M, additional, Frajman, E, additional, Milchman, C, additional, O'Regan, A, additional, Labuschagne, I, additional, Stout, J, additional, Landwehrmeyer, B, additional, Craufurd, D, additional, Scahill, R, additional, Hicks, S, additional, Kennard, C, additional, Johnson, H, additional, Tobin, A, additional, Rosas, HD, additional, Reilmann, R, additional, Borowsky, B, additional, Pourchot, C, additional, Andrews, S C, additional, Bachoud-Lévi, Anne-Catherine, additional, Bentivoglio, Anna Rita, additional, Biunno, Ida, additional, Bonelli, Raphael, additional, Burgunder, Jean-Marc, additional, Dunnett, Stephen, additional, Ferreira, Joaquim, additional, Handley, Olivia, additional, Heiberg, Arvid, additional, Illmann, Torsten, additional, Landwehrmeyer, G. Bernhard, additional, Levey, Jamie, additional, Ramos-Arroyo, Maria A., additional, Nielsen, Jørgen, additional, Koivisto, Susana Pro, additional, Päivärinta, Markku, additional, Roos, Raymund A.C., additional, Sebastián, A Rojo, additional, Tabrizi, Sarah, additional, Vandenberghe, Wim, additional, Verellen-Dumoulin, Christine, additional, Uhrova, Tereza, additional, Wahlström, Jan, additional, Zaremba, Jacek, additional, Baake, Verena, additional, Barth, Katrin, additional, Garde, Monica Bascuñana, additional, Betz, Sabrina, additional, Bos, Reineke, additional, Callaghan, Jenny, additional, Come, Adrien, additional, Guedes, Leonor Correia, additional, Ecker, Daniel, additional, Finisterra, Ana Maria, additional, Fullam, Ruth, additional, Gilling, Mette, additional, Gustafsson, Lena, additional, Handley, Olivia J, additional, Hvalstedt, Carina, additional, Held, Christine, additional, Koppers, Kerstin, additional, Lamanna, Claudia, additional, Laurà, Matilde, additional, Descals, Asunción Martínez, additional, Martinez-Horta, Saül, additional, Mestre, Tiago, additional, Minster, Sara, additional, Monza, Daniela, additional, Mütze, Lisanne, additional, Oehmen, Martin, additional, Orth, Michael, additional, Padieu, Hélène, additional, Paterski, Laurent, additional, Peppa, Nadia, additional, Di Renzo, Martina, additional, Rialland, Amandine, additional, Røren, Niini, additional, Šašinková, Pavla, additional, Timewell, Erika, additional, Townhill, Jenny, additional, Cubillo, Patricia Trigo, additional, da Silva, Wildson Vieira, additional, van Walsem, Marleen R, additional, Whalstedt, Carina, additional, Witjes-Ané, Marie-Noelle, additional, Witkowski, Grzegorz, additional, Wright, Abigail, additional, Zielonka, Daniel, additional, Zielonka, Eugeniusz, additional, Zinzi, Paola, additional, Bonelli, Raphael M., additional, Lilek, Sabine, additional, Hecht, Karen, additional, Herranhof, Brigitte, additional, Holl, Anna, additional, Kapfhammer, Hans-Peter, additional, Koppitz, Michael, additional, Magnet, Markus, additional, Müller, Nicole, additional, Otti, Daniela, additional, Painold, Annamaria, additional, Reisinger, Karin, additional, Scheibl, Monika, additional, Schöggl, Helmut, additional, Ullah, Jasmin, additional, Braunwarth, Eva-Maria, additional, Brugger, Florian, additional, Buratti, Lisa, additional, Hametner, Eva-Maria, additional, Hepperger, Caroline, additional, Holas, Christiane, additional, Hotter, Anna, additional, Hussl, Anna, additional, Müller, Christoph, additional, Poewe, Werner, additional, Seppi, Klaus, additional, Sprenger, Fabienne, additional, Wenning, Gregor, additional, Boogaerts, Andrea, additional, Calmeyn, Godelinde, additional, Delvaux, Isabelle, additional, Liessens, Dirk, additional, Somers, Nele, additional, Dupuit, Michel, additional, Minet, Cécile, additional, van Paemel, Dominique, additional, Ribaï, Pascale, additional, van Reijen, Dimphna, additional, Klempír, Jirí, additional, Majerová, Veronika, additional, Roth, Jan, additional, Stárková, Irena, additional, Hjermind, Lena E., additional, Jacobsen, Oda, additional, Nielsen, Jørgen E., additional, Larsen, Ida Unmack, additional, Vinther-Jensen, Tua, additional, Hiivola, Heli, additional, Hyppönen, Hannele, additional, Martikainen, Kirsti, additional, Tuuha, Katri, additional, Allain, Philippe, additional, Bonneau, Dominique, additional, Bost, Marie, additional, Gohier, Bénédicte, additional, Guérid, Marie-Anne, additional, Olivier, Audrey, additional, Prundean, Adriana, additional, Scherer-Gagou, Clarisse, additional, Verny, Christophe, additional, Babiloni, Blandine, additional, Debruxelles, Sabrina, additional, Duché, Charlotte, additional, Goizet, Cyril, additional, Jameau, Laetitia, additional, Lafoucrière, Danielle, additional, Spampinato, Umberto, additional, Barthélémy, Rekha, additional, De Bruycker, Christelle, additional, Carette, Maryline Cabaret Anne-Sophie, additional, Defebvre, Eric Decorte Luc, additional, Delliaux, Marie, additional, Delval, Arnaud, additional, Destee, Alain, additional, Dujardin, Kathy, additional, Lemaire, Marie-Hélène, additional, Manouvrier, Sylvie, additional, Peter, Mireille, additional, Plomhouse, Lucie, additional, Sablonnière, Bernard, additional, Simonin, Clémence, additional, Thibault-Tanchou, Stéphanie, additional, Vuillaume, Isabelle, additional, Bellonet, Marcellin, additional, Berrissoul, Hassan, additional, Blin, Stéphanie, additional, Courtin, Françoise, additional, Duru, Cécile, additional, Fasquel, Véronique, additional, Godefroy, Olivier, additional, Krystkowiak, Pierre, additional, Mantaux, Béatrice, additional, Roussel, Martine, additional, Wannepain, Sandrine, additional, Azulay, Jean-Philippe, additional, Delfini, Marie, additional, Eusebio, Alexandre, additional, Fluchere, Frédérique, additional, Mundler, Laura, additional, Anheim, Mathieu, additional, Julié, Celine, additional, Boukbiza, Ouhaid Lagha, additional, Longato, Nadine, additional, Rudolf, Gabrielle, additional, Tranchant, Christine, additional, Zimmermann, Marie-Agathe, additional, Kosinski, Christoph Michael, additional, Milkereit, Eva, additional, Probst, Daniela, additional, Reetz, Kathrin, additional, Sass, Christian, additional, Schiefer, Johannes, additional, Schlangen, Christiane, additional, Werner, Cornelius J., additional, Gelderblom, Harald, additional, Priller, Josef, additional, Prüß, Harald, additional, Spruth, Eike Jakob, additional, Ellrichmann, Gisa, additional, Herrmann, Lennard, additional, Hoffmann, Rainer, additional, Kaminski, Barbara, additional, Kotz, Peter, additional, Prehn, Christian, additional, Saft, Carsten, additional, Lange, Herwig, additional, Maiwald, Robert, additional, Löhle, Matthias, additional, Maass, Antonia, additional, Schmidt, Simone, additional, Bosredon, Cecile, additional, Storch, Alexander, additional, Wolz, Annett, additional, Wolz, Martin, additional, Capetian, Philipp, additional, Lambeck, Johann, additional, Zucker, Birgit, additional, Boelmans, Kai, additional, Ganos, Christos, additional, Heinicke, Walburgis, additional, Hidding, Ute, additional, Lewerenz, Jan, additional, Münchau, Alexander, additional, Schmalfeld, Jenny, additional, Stubbe, Lars, additional, Zittel, Simone, additional, Diercks, Gabriele, additional, Dressler, Dirk, additional, Gorzolla, Heike, additional, Schrader, Christoph, additional, Tacik, Pawel, additional, Ribbat, Michael, additional, Longinus, Bernhard, additional, Bürk, Katrin, additional, Möller, Jens Carsten, additional, Rissling, Ida, additional, Mühlau, Mark, additional, Peinemann, Alexander, additional, Städtler, Michael, additional, Weindl, Adolf, additional, Winkelmann, Juliane, additional, Ziegler, Cornelia, additional, Bechtel, Natalie, additional, Beckmann, Heike, additional, Bohlen, Stefan, additional, Hölzner, Eva, additional, Reilmann, Ralf, additional, Rohm, Stefanie, additional, Rumpf, Silke, additional, Schepers, Sigrun, additional, Weber, Natalia, additional, Dose, Matthias, additional, Leythäuser, Gabriele, additional, Marquard, Ralf, additional, Raab, Tina, additional, Wiedemann, Alexandra, additional, Buck, Andrea, additional, Connemann, Julia, additional, Geitner, Carolin, additional, Kesse, Andrea, additional, Landwehrmeyer, Bernhard, additional, Lang, Christina, additional, Lezius, Franziska, additional, Nepper, Solveig, additional, Niess, Anke, additional, Schneider, Ariane, additional, Schwenk, Daniela, additional, Süßmuth, Sigurd, additional, Trautmann, Sonja, additional, Weydt, Patrick, additional, Cormio, Claudia, additional, Sciruicchio, Vittorio, additional, Serpino, Claudia, additional, de Tommaso, Marina, additional, Capellari, Sabina, additional, Cortelli, Pietro, additional, Galassi, Roberto, additional, Rizzo, Giovanni, additional, Poda, Roberto, additional, Scaglione, Cesa, additional, Bertini, Elisabetta, additional, Ghelli, Elena, additional, Ginestroni, Andrea, additional, Massaro, Francesca, additional, Mechi, Claudia, additional, Paganini, Marco, additional, Piacentini, Silvia, additional, Pradella, Silvia, additional, Romoli, Anna Maria, additional, Sorbi, Sandro, additional, Abbruzzese, Giovanni, additional, di Poggio, Monica Bandettini, additional, Ferrandes, Giovanna, additional, Mandich, Paola, additional, Marchese, Roberta, additional, Albanese, Alberto, additional, Di Bella, Daniela, additional, Castaldo, Anna, additional, Di Donato, Stefano, additional, Gellera, Cinzia, additional, Genitrini, Silvia, additional, Mariotti, Caterina, additional, Nanetti, Lorenzo, additional, Paridi, Dominga, additional, Soliveri, Paola, additional, Tomasello, Chiara, additional, De Michele, Giuseppe, additional, Di Maio, Luigi, additional, Massarelli, Marco, additional, Peluso, Silvio, additional, Roca, Alessandro, additional, Russo, Cinzia Valeria, additional, Salvatore, Elena, additional, Sorrentino, Pierpaolo, additional, Amico, Enrico, additional, Favellato, Mariagrazia, additional, Griguoli, Annamaria, additional, Mazzante, Irene, additional, Petrollini, Martina, additional, Squitieri, Ferdinando, additional, D'Alessio, Barbara, additional, Esposito, Chiara, additional, Bentivoglio, Rita, additional, Frontali, Marina, additional, Guidubaldi, Arianna, additional, Ialongo, Tamara, additional, Jacopini, Gioia, additional, Piano, Carla, additional, Romano, Silvia, additional, Soleti, Francesco, additional, Spadaro, Maria, additional, van Hout, Monique S.E., additional, Verhoeven, Marloes E., additional, van Vugt, Jeroen P.P., additional, de Weert, A. Marit, additional, Bolwijn, J.J.W., additional, Dekker, M., additional, Kremer, B., additional, Leenders, K.L., additional, van Oostrom, J.C.H., additional, van den Bogaard, Simon J.A., additional, Dumas, Eve M., additional, 't Hart, Ellen P., additional, Kremer, Berry, additional, Verstappen, C.C.P., additional, Aaserud, Olaf, additional, C, Jan Frich, additional, Wehus, Ragnhild, additional, Bjørgo, Kathrine, additional, Fannemel, Madeleine, additional, Gørvell, Per F., additional, Lorentzen, Eirin, additional, Retterstøl, Lars, additional, Stokke, Bodil, additional, Bjørnevoll, Inga, additional, Sando, Sigrid Botne, additional, Dziadkiewicz, Artur, additional, Nowak, Malgorzata, additional, Robowski, Piotr, additional, Sitek, Emilia, additional, Slawek, Jaroslaw, additional, Soltan, Witold, additional, Szinwelski, Michal, additional, Blaszcyk, Magdalena, additional, Boczarska-Jedynak, Magdalena, additional, Ciach-Wysocka, Ewelina, additional, Gorzkowska, Agnieszka, additional, Jasinska-Myga, Barbara, additional, Klodowska-Duda, Gabriela, additional, Opala, Gregorz, additional, Stompel, Daniel, additional, Banaszkiewicz, Krzysztof, additional, Bocwinska, Dorota, additional, Bojakowska-Jaremek, Kamila, additional, Dec, Malgorzata, additional, Krawczyk, Malgorzata, additional, Rudzinska, Monika, additional, Szczygiel, Elzbieta, additional, Szczudlik, Andrzej, additional, Wasielewska, Anna, additional, Wójcik, Magdalena, additional, Bryl, Anna, additional, Ciesielska, Anna, additional, Klimberg, Aneta, additional, Marcinkowski, Jerzy, additional, Samara, Husam, additional, Sempolowicz, Justyna, additional, Gogol, Anna, additional, Janik, Piotr, additional, Kwiecinski, Hubert, additional, Jamrozik, Zygmunt, additional, Antczak, Jakub, additional, Jachinska, Katarzyna, additional, Krysa, Wioletta, additional, Rakowicz, Maryla, additional, Richter, Przemyslaw, additional, Rola, Rafal, additional, Ryglewicz, Danuta, additional, Sienkiewicz-Jarosz, Halina, additional, Stepniak, Iwona, additional, Sulek, Anna, additional, Zdzienicka, Elzbieta, additional, Zieora-Jakutowicz, Karolina, additional, Ferreira, Joaquim J, additional, Coelho, Miguel, additional, Mendes, Tiago, additional, Valadas, Anabela, additional, Andrade, Carlos, additional, Gago, Miguel, additional, Garrett, Carolina, additional, Guerra, Maria Rosália, additional, Herrera, Carmen Durán, additional, Garcia, Patrocinio Moreno, additional, Barbera, Miquel Aguilar, additional, Guia, Dolors Badenes, additional, Hernanz, Laura Casas, additional, Catena, Judit López, additional, Ferrer, Pilar Quiléz, additional, Sebastián, Ana Rojo, additional, Carruesco, Gemma Tome, additional, Bas, Jordi, additional, Busquets, Núria, additional, Calopa, Matilde, additional, Robert, Misericordia Floriach, additional, Viladrich, Celia Mareca, additional, Idiago, Jesús Miguel Ruiz, additional, Riballo, Antonio Villa, additional, Cubo, Esther, additional, Polo, Cecilia Gil, additional, Mariscal, Natividad, additional, Rivadeneyra, Perez Jessica, additional, Barrero, Francisco, additional, Morales, Blas, additional, Fenollar, María, additional, García, Rocío García-Ramos, additional, Ortega, Paloma, additional, Villanueva, Clara, additional, Alegre, Javier, additional, Bascuñana, Mónica, additional, Caldentey, Juan Garcia, additional, Ventura, Marta Fatás, additional, Ribas, Guillermo García, additional, de Yébenes, Justo García, additional, Moreno, José Luis López-Sendón, additional, Frech, Fernando Alonso, additional, Ruíz, Pedro J García, additional, Martínez-Descals, Asunción, additional, Guerrero, Rosa, additional, Artiga, María José Saiz, additional, Sánchez, Vicenta, additional, Perea, María Fuensanta Noguera, additional, Fortuna, Lorenza, additional, Manzanares, Salvadora, additional, Reinante, Gema, additional, Torres, María Martirio Antequera, additional, Moreau, Laura Vivancos, additional, González González, Sonia, additional, Guisasola, Luis Menéndez, additional, Salvador, Carlos, additional, Martín, Esther Suaréz San, additional, Ramirez, Inés Legarda, additional, Gorospe, Aranzazú, additional, Lopera, Mónica Rodriguez, additional, Arques, Penelope Navas, additional, Rodríguez, María José Torres, additional, Pastor, Barbara Vives, additional, Gaston, Itziar, additional, Martinez-Jaurrieta, Maria Dolores, additional, Moreno, Jose Manuel Garcia, additional, Lucena, Carolina Mendez, additional, Damas, Fatima, additional, Cortegana, Hermoso Eva Pacheco, additional, Peña, José Chacón, additional, Redondo, Luis, additional, Carrillo, Fátima, additional, Teresa Cáceres, María, additional, Mir, Pablo, additional, Suarez, María José Lama, additional, Vargas-González, Laura, additional, Bosca, Maria E., additional, Brugada, Francisco Castera, additional, Burguera, Juan Andres, additional, Campos, Anabel, additional, Vilaplana, Garcia Carmen Peiró, additional, Berglund, Peter, additional, Constantinescu, Radu, additional, Fredlund, Gunnel, additional, Høsterey-Ugander, Ulrika, additional, Linnsand, Petra, additional, Neleborn-Lingefjärd, Liselotte, additional, Wentzel, Magnus, additional, Loutfi, Ghada, additional, Olofsson, Carina, additional, Stattin, Eva-Lena, additional, Westman, Laila, additional, Wikström, Birgitta, additional, Stebler, Yanik, additional, Kaelin, Alain, additional, Romero, Irene, additional, Schüpbach, Michael, additional, Weber Zaugg, Sabine, additional, Hauer, Maria, additional, Gonzenbach, Roman, additional, Jung, Hans H., additional, Mihaylova, Violeta, additional, Petersen, Jens, additional, Jack, Roisin, additional, Matheson, Kirsty, additional, Miedzybrodzka, Zosia, additional, Rae, Daniela, additional, Simpson, Sheila A, additional, Summers, Fiona, additional, Ure, Alexandra, additional, Vaughan, Vivien, additional, Akhtar, Shahbana, additional, Crooks, Jenny, additional, Curtis, Adrienne, additional, de Souza, Jenny, additional, Piedad, John, additional, Rickards, Hugh, additional, Wright, Jan, additional, Coulthard, Elizabeth, additional, Gethin, Louise, additional, Hayward, Beverley, additional, Sieradzan, Kasia, additional, Armstrong, Matthew, additional, Barker, Roger A., additional, O'Keefe, Deidre, additional, Di Pietro, Anna, additional, Fisher, Kate, additional, Goodman, Anna, additional, Hill, Susan, additional, Kershaw, Ann, additional, Mason, Sarah, additional, Paterson, Nicole, additional, Raymond, Lucy, additional, Swain, Rachel, additional, Guzman, Natalie Valle, additional, Busse, Monica, additional, Butcher, Cynthia, additional, Clenaghan, Catherine, additional, Hunt, Sarah, additional, Jones, Una, additional, Khalil, Hanan, additional, Owen, Michael, additional, Price, Kathleen, additional, Rosser, Anne, additional, Edwards, Maureen, additional, Ho, Carrie, additional, Hughes, Teresa, additional, McGill, Marie, additional, Pearson, Pauline, additional, Porteous, Mary, additional, Smith, Paul, additional, Brockie, Peter, additional, Foster, Jillian, additional, Johns, Nicola, additional, McKenzie, Sue, additional, Rothery, Jean, additional, Thomas, Gareth, additional, Yates, Shona, additional, Burrows, Liz, additional, Chu, Carol, additional, Fletcher, Amy, additional, Gallantrae, Deena, additional, Hamer, Stephanie, additional, Harding, Alison, additional, Klöppel, Stefan, additional, Kraus, Alison, additional, Laver, Fiona, additional, Lewis, Monica, additional, Longthorpe, Mandy, additional, Markova, Ivana, additional, Raman, Ashok, additional, Robertson, Nicola, additional, Silva, Mark, additional, Thomson, Aileen, additional, Wild, Sue, additional, Yardumian, Pam, additional, Evans, Carole, additional, Gallentrae, Deena, additional, Hobson, Emma, additional, Jamieson, Stuart, additional, Musgrave, Hannah, additional, Rowett, Liz, additional, Toscano, Jean, additional, Bourne, Colin, additional, Clapton, Jackie, additional, Clayton, Carole, additional, Dipple, Heather, additional, Freire-Patino, Dawn, additional, Grant, Janet, additional, Gross, Diana, additional, Hallam, Caroline, additional, Middleton, Julia, additional, Murch, Ann, additional, Thompson, Catherine, additional, Alusi, Sundus, additional, Davies, Rhys, additional, Foy, Kevin, additional, Gerrans, Emily, additional, Pate, Louise, additional, Andrews, Thomasin, additional, Dougherty, Andrew, additional, Golding, Charlotte, additional, Kavalier, Fred, additional, Laing, Hana, additional, Lashwood, Alison, additional, Robertson, Dene, additional, Ruddy, Deborah, additional, Santhouse, Alastair, additional, Whaite, Anna, additional, Bruno, Stefania, additional, Doherty, Karen, additional, Haider, Salman, additional, Hensman, Davina, additional, Lahiri, Nayana, additional, Novak, Marianne, additional, Patel, Aakta, additional, Rosser, Elisabeth, additional, Taylor, Rachel, additional, Warner, Thomas, additional, Wild, Edward, additional, Arran, Natalie, additional, Bek, Judith, additional, Craufurd, David, additional, Hare, Marianne, additional, Howard, Liz, additional, Huson, Susan, additional, Johnson, Liz, additional, Jones, Mary, additional, Murphy, Helen, additional, Oughton, Emma, additional, Partington-Jones, Lucy, additional, Rogers, Dawn, additional, Sollom, Andrea, additional, Snowden, Julie, additional, Stopford, Cheryl, additional, Thompson, Jennifer, additional, Trender-Gerhard, Iris, additional, Verstraelen, Nichola, additional, Westmoreland, Leann, additional, Armstrong, Richard, additional, Dixon, Kathryn, additional, Nemeth, Andrea H, additional, Siuda, Gill, additional, Valentine, Ruth, additional, Harrison, David, additional, Hughes, Max, additional, Parkinson, Andrew, additional, Soltysiak, Beverley, additional, Bandmann, Oliver, additional, Bradbury, Alyson, additional, Gill, Paul, additional, Fairtlough, Helen, additional, Fillingham, Kay, additional, Foustanos, Isabella, additional, Kazoka, Mbombe, additional, O'Donovan, Kirsty, additional, Taylor, Cat, additional, Tidswell, Katherine, additional, Quarrell, Oliver, additional, Lau, Puay Ngoh, additional, Pica, Emmanul, additional, and Tan, Louis, additional

Published
2017
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40. Efficacy of repeated trickle applications of oxalic acid in syrup for varroosis control in Apis mellifera: Influence of meteorological conditions and presence of brood

Author

Iosif Papanastasiou, Costas J. Saitanis, Antonio Nanetti, Erifylli Roinioti, and N. Bacandritsos

Subjects
Sucrose, Apiary, Oxalic acid, Biology, Insect Control, Toxicology, chemistry.chemical_compound, Botany, Mite, Animals, Mortality, Sugar, Mites, Dose-Response Relationship, Drug, Greece, General Veterinary, business.industry, Oxalic Acid, Reproduction, Pest control, General Medicine, Bees, biology.organism_classification, Brood, chemistry, Reducing Agents, Varroa destructor, Parasitology, Pest Control, Seasons, business
Abstract

Oxalic acid field trails for the control of varroosis (Varroa destructor) were carried out in an apiary located on the Mt. Imittos (Attica, Greece). The colonies received four successive applications (approximately one every 16 days) with 4.2% oxalic acid (OA) and 60% sugar solution by trickling method with two alternative types of syringes (an automatic self-filling dosing and a single-use) from the broodright to broodless period. The results indicate that the first three applications (from 6th October to 25th November-broodright period) resulted in 65.3% cumulative mite mortality, while only the last application (after the 26th November-broodless period) resulted in 77.3% mite mortality. Very low outern temperatures reduce to the minimum the bee movability, which may result into a slower development of the OA efficacy. No poor colony growth or queen loss were observed even if the bee colonies were received the four successive OA applications with the last one taken place at a very low outern temperature (6.2 degrees C). The trickling method using an automatic-filling syringe seems to be a very quick way for applying oxalic acid in large apiaries (approximately 150hives/h).

Published
2007
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41. Peroxynitrite production and NOS expression in astrocytes U373MG incubated with lipoproteins from Alzheimer patients

Author

Laura Nanetti, Cinzia Moroni, Simona Luzzi, M. Bartolini, Laura Mazzanti, Leandro Provinciali, and Arianna Vignini

Subjects
Male, Apolipoprotein E, medicine.medical_specialty, Lipoproteins, Blotting, Western, Nitric Oxide Synthase Type II, Inflammation, Models, Biological, Cell Line, Nitric oxide, chemistry.chemical_compound, Alzheimer Disease, Peroxynitrous Acid, Internal medicine, medicine, Humans, Molecular Biology, Nitrites, Aged, Aged, 80 and over, biology, General Neuroscience, Cholesterol, HDL, Cholesterol, LDL, Middle Aged, Nitric oxide synthase, medicine.anatomical_structure, Endocrinology, Gene Expression Regulation, chemistry, Astrocytes, Case-Control Studies, Immunology, biology.protein, Neuroglia, Female, Neurology (clinical), Nitric Oxide Synthase, medicine.symptom, Peroxynitrite, Developmental Biology, Lipoprotein, Astrocyte
Abstract

Apolipoprotein E (apo E), a plasma protein involved both in the metabolism of cholesterol and triglycerides, particularly in nervous tissue, has been associated with a higher risk of Alzheimer's disease. It has been shown that apo E increased the production of nitric oxide (NO) from human monocyte-derived macrophages (MDM); this effect could represent an important link between tissue redox balance and inflammation, since inflammation and oxidative stress are involved in chronic neurodegenerative disorders. Moreover, it has been evidenced that an overproduction of NO in the central nervous system (CNS) may play a key role in aging and that the glial cells (microglials cells and probably astrocytes) are able to form consistent amounts of NO through the induction of a nitric oxide synthase (iNOS) isoform so-called inducible or inflammatory. This report was performed in order to elucidate the effects produced by lipoproteins from control subjects, AD patients and first degree relatives (offspring) on human astrocyte cells after a short incubation. Peroxynitrite and NO production and NOS expression in cultured astrocytes were measured. We observed a decreased NO production after incubation with both LDL and HDL and an increased peroxynitrite production. As it concerns NOS expression, densitometric analysis of bands indicated that iNOS protein levels were significantly higher in the cells incubated with both AD lipoproteins and offspring lipoproteins compared to cells incubated with control lipoproteins. These findings suggest the possibility to identify in NO pathway a precocious marker of AD.

Published
2005
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42. Investigation of defects in Fresco substrates by means of the ECoSp imaging system and the principal component image analysis

Author

E. Lodi, N. Ludwig, M. Milazzo, Claudio Bonifazzi, G. Maino, Agostino Tartari, Luca Nanetti, and Valerio Muzzioli

Subjects
Nuclear and High Energy Physics, Photon, Materials science, Spectrometer, business.industry, Scattering, Compton scattering, Sample (graphics), Particle detector, Optics, Principal component analysis, Measuring instrument, business, Instrumentation
Abstract

A new imaging technique is presented, based on the detection of Compton scattering photons and a further statistical analysis of the collected data. The photon detection has been performed by the Enhanced Compton Spectrometer (ECoSp) a recently devised instrument that allows one to collect the backscattered photons by investigating the tested sample from one side only. Photons collected during the planar scanning of a given area are used to describe the electronic density of the sample as a density image; afterwards, the Principal Component Analysis (PCA) was applied to the image. As a case study, a non-destructive testing of plaster substrates supporting mural paintings has been performed using this technique, searching for flaws, defects, fractures and so on. The rationale behind this procedure is that the presence of such gaps, into the otherwise uniform material, can be revealed because the existing correlation between the backscattering radiation detected by ECoSp from each adjacent volume of interest (VOI) during the scanning. The principal component correlation analysis, performed over the resulting data from all VOIs, then reveals each gap in his size, shape and position.

Published
2004
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43. Description of the response in scintillation crystal arrays: analytical versus statistical approaches

Author

M.N. Cinti, Claudio Bonifazzi, Roberto Pani, Agostino Tartari, Luca Nanetti, and Rosanna Pellegrini

Subjects
Physics, Nuclear and High Energy Physics, Scintillation, business.industry, Detector, Linearity, Particle detector, Crystal, Optics, Distortion, Scintillation counter, Measuring instrument, business, Instrumentation
Abstract

An analytical and a statistical approach to describe the scintillation crystal arrays responses are presented in order to identify crystal detectors. They were applied to two flood-field irradiation images, with and without strong distortion and no linearity. The comparison of the outcomes suggests that the first and simplest to apply one is appropriate for light distortion, whereas the second and more complex statistical approach is unavoidable in the presence of strong distortions of the image.

Published
2004
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44. Reduced susceptibility to peroxidation of erythrocyte plasma membranes from centenarians

Author

Claudio Franceschi, Laura Nanetti, Rosa Anna Rabini, N. Moretti, Eleonora Salvolini, Laura Mazzanti, and R. Staffolani

Subjects
Adult, Lipid Peroxides, Aging, medicine.medical_specialty, Membrane Fluidity, Biochemistry, Lipid peroxidation, Membrane Lipids, chemistry.chemical_compound, Endocrinology, Internal medicine, Genetics, medicine, Membrane fluidity, Humans, Molecular Biology, Aged, Aged, 80 and over, chemistry.chemical_classification, Lipid peroxide, Chemistry, Erythrocyte Membrane, Fatty Acids, Cell Biology, Middle Aged, Malondialdehyde, Docosahexaenoic acid, Saturated fatty acid, Arachidonic acid, Lipid Peroxidation, Polyunsaturated fatty acid
Abstract

The plasma membrane composition affects intracellular processes and the cellular susceptibility to free radical attack, which has been associated with the impairment of cellular functions occurring during senescence. The study of the modifications of the plasma membrane in centenarians might elucidate the biological mechanisms at the basis of longevity and successful aging. The work was performed in 190 subjects, divided into five groups according to the age range: (1) 21-40 years (n=25); (2) 41-60 years (n=30); (3) 61-80 years (n=30); (4) 81-99 years (n=50); and (5) centenarians (> or = 100 years) (n=55). The following determinations were performed on erythrocyte membranes: (i) the lipid peroxide level (Lp) evaluated as malondialdehyde content; (ii) susceptibility to in vitro oxidation evaluated as difference in the content of thiobarbituric acid-reactive substances before and after phenylhydrazine addition; (iii) unsaturated/saturated fatty acid ratio and individual polyunsaturated fatty acid composition measured by gas chromatography; and (iv) fluidity studied by means of the anisotropy of the probe 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). Erythrocyte membranes from centenarians showed: (i) decreased basal lipid peroxide levels and reduced susceptibility to peroxidation in comparison with elderly subjects; (ii) increased unsaturated/saturated fatty acid ratio in comparison with every other age group; (iii) higher levels of eicosapentaenoic and docosahexaenoic acid and reduced content of linoleic and arachidonic acid in comparison with elderly subjects; and (iv) decreased anisotropy of TMA-DPH, i.e. higher fluidity compared with all the other age groups. In conclusion, the present work demonstrates that erythrocyte membranes from centenarians show some distinct features in comparison with elderly subjects that might act in a protective way against injuries.

Published
2002
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45. Sialic acid content in erythrocyte membranes from pregnant women affected by gestational diabetes

Author

Laura Nanetti, Laura Mazzanti, Rosa Anna Rabini, M.C. Curzi, N. Cester, L.A. Tranquilli, N. Moretti, and G. Grechi

Subjects
Adult, medicine.medical_specialty, Membrane Fluidity, Endocrinology, Diabetes and Metabolism, ATPase, Blood viscosity, Fluorescence Polarization, chemistry.chemical_compound, Endocrinology, Pregnancy, Diabetes mellitus, Internal medicine, medicine, Membrane fluidity, Humans, biology, Erythrocyte Membrane, medicine.disease, N-Acetylneuraminic Acid, Sialic acid, Gestational diabetes, Diabetes, Gestational, chemistry, biology.protein, Female, Triphosphatase, Sodium-Potassium-Exchanging ATPase, N-Acetylneuraminic acid
Abstract

Sialic acid (SA) content, membrane fluidity, and Na(+)/K(+)-adenosine triphosphatase (ATPase) activity were determined in erythrocyte membrane from 10 nonpregnant women (HNPW), 16 pregnant women affected by gestational diabetes mellitus (GDM), and 25 healthy pregnant women (HPW). In GDM patients the membrane erythrocyte SA content was significantly increased compared with HNPW and membrane fluidity was significantly increased in comparison with HPW. Erythrocyte membrane Na(+)/K(+)-ATPase activity was significantly reduced in GDM patients compared both to HNPW and to HPW subjects. A significant inverse correlation was found between 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) anisotropy and erythrocyte membrane SA content in HNPW and in HPW, while this significant correlation was not observed in GDM. The present results indicate that in comparison with normal pregnancy GDM is characterized by deep alterations of the erythrocyte plasma membrane physicochemical properties (increased fluidity) and functional activities (reduced Na(+)/K(+)-ATPase activity). These modifications might be at the basis of the altered blood viscosity and placental perfusion observed under such conditions. Moreover, these results show that in physiological pregnancy and in the nonpregnant state, the erythrocyte surface membrane fluidity is inversely correlated with SA content, while in GDM there is an unbalance of this relation, which might be associated with the microcirculatory abnormality present in this disease.

Published
2002
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46. Co-occurrence of amyotrophic lateral sclerosis and Charcot-Marie-Tooth disease type 2A in a patient with a novel mutation in the mitofusin-2 gene

Author

Davide Pareyson, Franco Taroni, Chiara Marchesi, Gian Maria Fabrizi, Claudia Ciano, Cinzia Gellera, Ettore Salsano, Antonino Uncini, M Milani, and Lorenzo Nanetti

Subjects
Pathology, medicine.medical_specialty, Weakness, Hearing loss, Comorbidity, Audiology, Charcot-Marie-Tooth neuropathy, Amyotrophic Lateral Sclerosis, CMT2A, Mitofusin-2, Motor neuron disease, GTP Phosphohydrolases, Mitochondrial Proteins, Fasciculation, Dysarthria, Atrophy, Charcot-Marie-Tooth Disease, medicine, Humans, Amyotrophic lateral sclerosis, Genetics (clinical), business.industry, Membrane Proteins, Middle Aged, Motor neuron, medicine.disease, Dysphagia, medicine.anatomical_structure, Neurology, Mutation, Pediatrics, Perinatology and Child Health, Disease Progression, Female, Neurology (clinical), medicine.symptom, business
Abstract

Mitofusin-2 gene (MFN2) mutations cause Charcot-Marie-Tooth type 2A (CMT2A), sometimes complicated by additional features such as optic atrophy, hearing loss, upper motor neuron signs and cerebral white-matter abnormalities. Here we report, for the first time, the occurrence of motor neuron disease, consistent with amyotrophic lateral sclerosis (ALS), in a 62-year-old woman affected by early-onset slowly progressive CMT2A, due to a novel MFN2 mutation. After age 60, rate of disease progression changed and she rapidly developed generalised muscle wasting, weakness, and fasciculations, together with dysarthria and dysphagia. Clinical features, EMG findings, and fast progression were consistent with ALS superimposed on CMT.

Published
2011
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47. Fiavobacteriurrt spp. organisms as opportunistic bacterial pathogens during advanced HIV disease

Author

Anna Nanetti, A. Mastroianni, Roberto Manfredi, Francesco Chiodo, O. Coronado, and M. Ferri

Subjects
Microbiology (medical), Opportunistic infection, medicine.drug_class, Antibiotics, Microbial Sensitivity Tests, Flavobacterium, Microbiology, Risk Factors, Sepsis, Pneumonia, Bacterial, medicine, Humans, Immunodeficiency, AIDS-Related Opportunistic Infections, biology, medicine.disease, biology.organism_classification, Antimicrobial, Anti-Bacterial Agents, Ciprofloxacin, Infectious Diseases, Absolute neutrophil count, Viral disease, Gram-Negative Bacterial Infections, medicine.drug
Abstract

Objective: To assess the role of Flavobacterium spp. infection in patients with HIV disease. Methods: Clinical charts of 2412 consecutive HIV-infected patients hospitalized in a 8-year period were retrospectively reviewed, to identify all cases of Flavobacterium spp. infections, and to evaluate their occurrence and outcome according to several epidemiological, clinical, and laboratory parameters. Results: Six patients out of 2412 (0.25%,), developed Flavobacterium spp. complications: septicaeama in five cases, and pneumonia in the remaining patient, with F. meningosepticum and F. odoratum isolated in two cases and one case, respectively, and unnamed Flavobacterium spp. organisms in the remaining three cases. Flavobacterium spp. organisms were responsible for six out of 1939 overall episodes of non-mycobacterial bacterial diseases observed in our patient group (0.31%). All patients were severely immunocompromised, showing a prior diagnosis of AIDS, a mean CD4+ lymphocyte count of 64.2 (range 12–187) cells/μl, and a mean neutrophil count of 1.143 (range 700–1600) cells (range 700–1600) cells/μl. Antibiotic, corticosteriod, or cotrimoxazole treatment was carried out during the month preceding disease onset by three, two and five patients, respectively Community-acquired and nosocomial Flavobacterium spp. disease were equally frequent, but the latter occurred with a significantly lower mean neutrophil and CD4+ cell count. Antimicrobial susceptibility assays showed complete sensitivity to ciprofloxacin, and variable resistance to ureidopenicillins, ceftazidirne, imipenem, aztreonam, and aminoglycosides. An appropriate antimicrobial regimen obtained clinical and microbiological cure in all cases, in absence of related mortality or relapses. Conclusions: Since only one episode of HIV-associated F. (Sphingobacterium) multivorum complication has been described to date, our series represents the largest one dealing with Flavobacterium spp. infection in the setting of HIV disease. Our experience suggests that Flavobacterium spp. organisms may play a pathogenic role in patients with advanced HIV disease, even when some commonly recognized risk factors are lacking (i.e. indwelling catheters, instrumentation, IV drug abuse), while a very love CD4+ lymphocyte count, leukopaenia-neutropaenia, and concurrent AIDS-related infectious complications may act as important predisposing factors. In view of the infrequent occurrence of these infections, early suspicion is essential for both clinicians and microbiologists facing immunocompromised patients at risk for invasive bacterial complications. Flavobacterium spp. organisms should be taken into consideration as nosocomial- or community-acquired opportunistic pathogens, due to their relationship with advanced immunodeficiency and their elevated resistance to many antimicrobial agents commonly used against Cram-negative bacterial pathogens.

Published
1999
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48. Progression characteristics of the European Friedreich’s Ataxia Consortium for Translational Studies (EFACTS): a 2 year cohort study

Author

Reetz, Kathrin, primary, Dogan, Imis, additional, Hilgers, Ralf-Dieter, additional, Giunti, Paola, additional, Mariotti, Caterina, additional, Durr, Alexandra, additional, Boesch, Sylvia, additional, Klopstock, Thomas, additional, de Rivera, Francisco Javier Rodriguez, additional, Schöls, Ludger, additional, Klockgether, Thomas, additional, Bürk, Katrin, additional, Rai, Myriam, additional, Pandolfo, Massimo, additional, Schulz, Jörg B, additional, Nachbauer, Wolfgang, additional, Eigentler, Andreas, additional, Depondt, Chantal, additional, Benaich, Sandra, additional, Charles, Perrine, additional, Ewenczyk, Claire, additional, Monin, Marie-Lorraine, additional, Dafotakis, Manuel, additional, Fedosov, Kathrin, additional, Didszun, Claire, additional, Ermis, Ummehan, additional, Giordano, Ilaria A, additional, Timmann, Dagmar, additional, Karin, Ivan, additional, Neuhofer, Christiane, additional, Stendel, Claudia, additional, Müller vom Hagen, Jennifer, additional, Wolf, Julia, additional, Panzeri, Marta, additional, Nanetti, Lorenzo, additional, Castaldo, Anna, additional, Arpa, Javier, additional, Sanz-Gallego, Irene, additional, Parkinson, Michael H, additional, and Sweeney, Mary G, additional

Published
2016
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49. Matching between virtual image and real robot world in robot-assisted surgery

Author

Paolo Dario, A. Visani, Maurilio Marcacci, T. Wang, S. Martelli, M. Nanetti, and M. Fadda

Subjects
Set (abstract data type), Engineering, Matching (statistics), Robotic systems, Software, SIMPLE (military communications protocol), Virtual image, business.industry, Calibration, Robot, Computer vision, Artificial intelligence, business
Abstract

This paper focuses on the problems of matching a virtual and a real environment by means of hardware and software tools. The real world is represented by a bone where we want to make a set of cuts by means of robotic system. The virtual world is a 3D model of the bone reconstructed from a set of CT slices. Robotic system is then here not only the instrument to machine bones but also the instrument to perform the fundamental step of registration between the two worlds. An external force sensor is used to adjust robot stiffness in order to perform the tactile searching necessary for the registration. A simple but reliable software algorithm is used to control the robot and hardware tools are self-made and designed. The results show the system proposed is precise enough for our application and tests we have made also clarified the way to improve it.

Published
1994
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50. Long-term disease progression in spinocerebellar ataxia types 1, 2, 3, and 6: a longitudinal cohort study

Author

Jacobi, Heike, primary, du Montcel, Sophie Tezenas, additional, Bauer, Peter, additional, Giunti, Paola, additional, Cook, Arron, additional, Labrum, Robyn, additional, Parkinson, Michael H, additional, Durr, Alexandra, additional, Brice, Alexis, additional, Charles, Perrine, additional, Marelli, Cecilia, additional, Mariotti, Caterina, additional, Nanetti, Lorenzo, additional, Panzeri, Marta, additional, Rakowicz, Maria, additional, Sulek, Anna, additional, Sobanska, Anna, additional, Schmitz-Hübsch, Tanja, additional, Schöls, Ludger, additional, Hengel, Holger, additional, Baliko, Laszlo, additional, Melegh, Bela, additional, Filla, Alessandro, additional, Antenora, Antonella, additional, Infante, Jon, additional, Berciano, José, additional, van de Warrenburg, Bart P, additional, Timmann, Dagmar, additional, Szymanski, Sandra, additional, Boesch, Sylvia, additional, Kang, Jun-Suk, additional, Pandolfo, Massimo, additional, Schulz, Jörg B, additional, Molho, Sonia, additional, Diallo, Alhassane, additional, and Klockgether, Thomas, additional

Published
2015
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