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Your search keyword '"Petrella, C"' showing total 22 results
Start Over Author "Petrella, C" Publisher elsevier bv
22 results on '"Petrella, C"'

1. Nerve growth factor derivative NGF61/100 promotes outgrowth of primary sensory neurons with reduced signs of nociceptive sensitization

Author

Severini, C., primary, Petrocchi Passeri, P., additional, Ciotti, M.T., additional, Florenzano, F., additional, Petrella, C., additional, Malerba, F., additional, Bruni, B., additional, D'Onofrio, M., additional, Arisi, I., additional, Brandi, R., additional, Possenti, R., additional, Calissano, P., additional, and Cattaneo, A., additional

Published
2017
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22. Nerve growth factor derivative NGF61/100 promotes outgrowth of primary sensory neurons with reduced signs of nociceptive sensitization

Author

M.T. Ciotti, Antonino Cattaneo, Ivan Arisi, B. Bruni, Roberta Possenti, Pietro Calissano, P. Petrocchi Passeri, Carla Petrella, Fulvio Florenzano, Mara D'Onofrio, Cinzia Severini, Rossella Brandi, Francesca Malerba, Severini, C, Petrocchi Passeri, P, Ciotti, M. T, Florenzano, F, Petrella, C, Malerba, Francesca, Bruni, B, D'Onofrio, M, Arisi, Ivan, Brandi, R, Possenti, R, Calissano, P, and Cattaneo, Antonino

Subjects
0301 basic medicine, genetic structures, Stimulation, Substance P, Tropomyosin receptor kinase A, chemistry.chemical_compound, 0302 clinical medicine, Dorsal root ganglion neurons, Ganglia, Spinal, Nerve Growth Factor, CGRP, NGF, biology, Bradykinin receptors, Bradykinin receptor, Up-Regulation, Nociception, Neurotrophin, medicine.medical_specialty, Sensory Receptor Cells, Calcitonin Gene-Related Peptide, Primary Cell Culture, TRPV1, Pain, TRPV Cation Channels, Calcitonin gene-related peptide, Bradykinin, Settore BIO/09, 03 medical and health sciences, Cellular and Molecular Neuroscience, Internal medicine, medicine, Animals, Humans, Pharmacology, business.industry, Gene Expression Profiling, Receptors, Bradykinin, Peptide Fragments, Rats, Dorsal root ganglion neuron, 030104 developmental biology, Endocrinology, Nerve growth factor, nervous system, chemistry, biology.protein, Calcium, business, Neuroscience, 030217 neurology & neurosurgery
Abstract

Nerve Growth Factor (NGF) is being considered as a therapeutic candidate for Alzheimer's disease. However, the development of an NGF-based therapy is limited by its potent pain activity. We have developed a “painless” derivative form of human NGF (NGF61/100), characterized by identical neurotrophic properties but a reduced nociceptive sensitization activity in vivo. Here we characterized the response of rat dorsal root ganglia neurons (DRG) to the NGF derivative NGF61/100, in comparison to that of control NGF (NGF61), analyzing the expression of noxious pro-nociceptive mediators. NGF61/100 displays a neurotrophic activity on DRG neurons comparable to that of control NGF61, despite a reduced activation of PLCγ, Akt and Erk1/2. NGF61/100 does not differ from NGF61 in its ability to up-regulate Substance P (SP) and Calcitonin Gene Related Peptide (CGRP) expression. However, upon Bradykinin (BK) stimulation, NGF61/100-treated DRG neurons release a much lower amount of SP and CGRP, compared to control NGF61 pre-treated neurons. This effect of painless NGF is explained by the reduced up-regulation of BK receptor 2 (B2R), respect to control NGF61. As a consequence, BK treatment reduced phosphorylation of the transient receptor channel subfamily V member 1 (TRPV1) in NGF61/100-treated cultures and induced a significantly lower intracellular Ca2+ mobilization, responsible for the lower release of noxious mediators. Transcriptomic analysis of DRG neurons treated with NGF61/100 or control NGF allowed identifying a small number of nociceptive-related genes that constitute an “NGF pain fingerprint”, whose differential regulation by NGF61/100 provides a strong mechanistic basis for its selective reduced pain sensitizing actions.

Published
2017
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