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1. Sequence analysis of respiratory syncytial virus cases reveals a novel subgroup -B strain circulating in north-central Italy after pandemic restrictions

Author

Pierangeli, Alessandra, primary, Midulla, Fabio, additional, Piralla, Antonio, additional, Ferrari, Guglielmo, additional, Nenna, Raffaella, additional, Pitrolo, Antonino Maria Guglielmo, additional, Licari, Amelia, additional, Marseglia, Gian Luigi, additional, Abruzzese, Dario, additional, Pellegrinelli, Laura, additional, Galli, Cristina, additional, Binda, Sandro, additional, Cereda, Danilo, additional, Fracella, Matteo, additional, Oliveto, Giuseppe, additional, Campagna, Roberta, additional, Petrarca, Laura, additional, Pariani, Elena, additional, Antonelli, Guido, additional, and Baldanti, Fausto, additional

Published
2024
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2. Impact of Whole Genome Sequencing to investigate transmission of Serratia marcescens in Neonatal Intensive Care Unit

Author

Cristina, Merla, primary, Marina, Ramus, additional, Angela, Kuka, additional, Irene, Mileto, additional, Stefano, Gaiarsa, additional, Amelia, Di Comite, additional, Marta, Corbella, additional, Antonio, Piralla, additional, Liliana, Lanave Marina, additional, Alba, Muzzi, additional, Stefano, Ghirardello, additional, Fausto, Baldanti, additional, and Patrizia, Cambieri, additional

Published
2024
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3. Dynamics of viral DNA shedding and culture viral DNA positivity in different clinical samples collected during the 2022 mpox outbreak in Lombardy, Italy

Author

Piralla, Antonio, primary, Mileto, Davide, additional, Rizzo, Alberto, additional, Ferrari, Guglielmo, additional, Giardina, Federica, additional, Gaiarsa, Stefano, additional, Petazzoni, Greta, additional, Bianchi, Micol, additional, Salari, Federica, additional, Bracchitta, Fiorenza, additional, Sammartino, Josè Camilla, additional, Ferrari, Alessandro, additional, Gagliardi, Gloria, additional, Mancon, Alessandro, additional, Fenizia, Claudio, additional, Biasin, Mara, additional, Rovida, Francesca, additional, Paolucci, Stefania, additional, Percivalle, Elena, additional, Lombardi, Alessandra, additional, Micheli, Valeria, additional, Nozza, Silvia, additional, Castagna, Antonella, additional, Moschese, Davide, additional, Antinori, Spinello, additional, Gori, Andrea, additional, Bonfanti, Paolo, additional, Rossotti, Roberto, additional, Monforte, Antonella D'arminio, additional, Attanasi, Federica, additional, Tirani, Marcello, additional, Cereda, Danilo, additional, Baldanti, Fausto, additional, Gismondo, Maria Rita, additional, Cutrera, Miriam, additional, Cuomo, Marianna, additional, De Poli, Federica, additional, Campanini, Giulia, additional, Pitrolo, Antonino Maria Guglielmo, additional, Iskandar, Elizabeth, additional, Cassaniti, Irene, additional, Bruno, Raffaele, additional, Rizzardini, Giuliano, additional, Puoti, Massimo, additional, Castelli, Francesco, additional, Corsico, Laura, additional, Giacomelli, Andrea, additional, Pozza, Giacomo, additional, Casalini, Giacomo, additional, Raccagni, Angelo, additional, Trentacapilli, Bendetta, additional, Bertoni, Costanza, additional, Bruzzesi, Elena, additional, Candela, Caterina, additional, Tesoro, Daniele, additional, Mule, Giovanni, additional, Bandera, Alessandra, additional, Bianca Mariani, Antonio Muscatello, additional, Maffeo, Manuel, additional, Vecchio, Riccardo, additional, and Piccinelli, Sara, additional

Published
2024
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4. Impact of SARS-CoV-2 Omicron and Delta variants in patients requiring intensive care unit (ICU) admission for COVID-19, Northern Italy, December 2021 to January 2022

Author

Piralla, Antonio, primary, Mojoli, Francesco, additional, Pellegrinelli, Laura, additional, Ceriotti, Ferruccio, additional, Valzano, Antonia, additional, Grasselli, Giacomo, additional, Gismondo, Maria Rita, additional, Micheli, Valeria, additional, Castelli, Antonio, additional, Farina, Claudio, additional, Arosio, Marco, additional, Lorini, Ferdinando Luca, additional, Fanti, Diana, additional, Busni, Andrea, additional, Laratta, Matteo, additional, Maggi, Fabrizio, additional, Novazzi, Federica, additional, Cabrini, Luca, additional, Callegaro, Anna Paola, additional, Keim, Roberto, additional, Remuzzi, Giuseppe, additional, Cavallero, Annalisa, additional, Malandrin, Sergio Maria Ivano, additional, Rona, Roberto, additional, Giardina, Federica, additional, Ferrari, Guglielmo, additional, Zavaglio, Federica, additional, D'angelo, Piera, additional, Galli, Cristina, additional, Bubba, Laura, additional, Binda, Sandro, additional, Oggioni, Massimo, additional, Uceda Renteria, Sara Colonia, additional, Bono, Patrizia, additional, Baj, Andreina, additional, Ferrante, Francesca Drago, additional, Guarneri, Davide, additional, Tonelli, Marco, additional, Napolitano, Gavino, additional, Nava, Alice, additional, Romeo, Lorenzo, additional, Nicolini, Elena, additional, Valaperta, Rea, additional, Varisano, Ludovica, additional, Mele, Caterina, additional, Liguori, Lucia, additional, Raggi, Monica, additional, Mongodi, Silvia, additional, Pagani, Michele, additional, Severgnini, Paolo, additional, Gasberti, Dario, additional, Bonanomi, Ezio, additional, Gritti, Paolo, additional, Marrazzo, Francesco, additional, Giovannini, Ilaria, additional, Sacchi, Noemi, additional, Sagliocco, Orlando, additional, Cereda, Danilo, additional, Buoro, Sabrina, additional, Baldanti, Fausto, additional, and Pariani, Elena, additional

Published
2023
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6. Vaccination for seasonal influenza, pneumococcal infection and SARS-CoV-2 in patients with solid tumors: recommendations of the Associazione Italiana di Oncologia Medica (AIOM)

Author

Pedrazzoli, P., primary, Lasagna, A., additional, Cassaniti, I., additional, Piralla, A., additional, Squeri, A., additional, Bruno, R., additional, Sacchi, P., additional, Baldanti, F., additional, Di Maio, M., additional, Beretta, G.D., additional, Cinieri, S., additional, and Silvestris, N., additional

Published
2023
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9. On the lookout for influenza viruses in Italy during the 2021-2022 season: Along came A(H3N2) viruses with a new phylogenetic makeup of their hemagglutinin

Author

Galli, Cristina, primary, Pellegrinelli, Laura, additional, Giardina, Federica, additional, Ferrari, Guglielmo, additional, Uceda Renteria, Sara Colonia, additional, Novazzi, Federica, additional, Masi, Elisa, additional, Pagani, Elisabetta, additional, Piccirilli, Giulia, additional, Mauro, Maria Vittoria, additional, Binda, Sandro, additional, Corvaro, Benedetta, additional, Tiberio, Claudia, additional, Lalle, Eleonora, additional, Maggi, Fabrizio, additional, Russo, Cristina, additional, Ranno, Stefania, additional, Vian, Elisa, additional, Pariani, Elena, additional, Baldanti, Fausto, additional, and Piralla, Antonio, additional

Published
2023
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10. EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

Author

Stefania Paolucci, Irene Cassaniti, Federica Novazzi, Loretta Fiorina, Antonio Piralla, Giuditta Comolli, Raffaele Bruno, Renato Maserati, Roberto Gulminetti, Stefano Novati, Francesco Mojoli, Fausto Baldanti, R Bruno, M Mondelli, E Brunetti, A Di Matteo, E Seminari, L Maiocchi, V Zuccaro, L Pagnucco, B Mariani, S Ludovisi, R Lissandrin, A Parisi, P Sacchi, SFA Patruno, G Michelone, R Gulminetti, D Zanaboni, S Novati, R Maserati, P Orsolini, M Vecchia, M Sciarra, E Asperges, M Colaneri, A Di Filippo, M Sambo, S Biscarini, M Lupi, S Roda, TC Pieri, I Gallazzi, M Sachs, P Valsecchi, S Perlini, C Alfano, M Bonzano, F Briganti, G Crescenzi, AG Falchi, R Guarnone, B Guglielmana, E Maggi, I Martino, P Pettenazza, S Pioli di Marco, F Quaglia, A Sabena, F Salinaro, F Speciale, I Zunino, M De Lorenzo, G Secco, L Dimitry, G Cappa, I Maisak, B Chiodi, M Sciarrini, B Barcella, F Resta, L Moroni, G Vezzoni, L Scattaglia, E Boscolo, C Zattera, MF Tassi, V Capozza, D Vignaroli, M Bazzini, G Iotti, F Mojoli, M Belliato, L Perotti, S Mongodi, G Tavazzi, G Marseglia, A Licari, I Brambilla, D Barbarini, A Bruno, P Cambieri, G Campanini, C. Cavanna, G Comolli, M Corbella, R Daturi, M Furione, P Marone, E Monzillo, S Paolucci, M Parea, E Percivalle, A Piralla, F Rovida, A Sarasini, M Zavattoni, G Adzasehoun, M Ardizzone, L Bellotti, V Brunco, E Cabano, G Casali, L Capella, D Devitis, L Dossena, G Frisco, G Garbagnoli, F Gardellini, A Girello, A Guerrizio, V Landini, C Lucchelli, V Maliardi, P Piemontese, S Pezzaia, M Premoli, C Rebuffa, C Zanello, J Bagnarino, F Bergami, A Bonetti, G Caneva, I Cassaniti, A Corcione, R Di Martino, A Di Napoli, A Ferrari, G Ferrari, L Fiorina, A Gallone, F Giardina, A Girardi, A Mercato, C Merla, F Novazzi, G Ratano, B Rossi, G Saveriaempillai, IM Sciabica, M Tallarita, E Vecchio Nepita, J Vitali, A Cerino, S Varchetta, B Oliviero, S Mantovani, D Mele, M Calvi, M Tizzoni, C Nicora, A Triarico, V Petronella, C Marena, A Muzzi, P Lago, S Cutti, V Novelli, F Comandatore, G BatistiBiffignandi, S Gaiarsa, M Rettani, and C Bandi

Subjects
Male, 0301 basic medicine, Human cytomegalovirus, Herpesvirus 4, Human, Epstein-Barr Virus Infections, viruses, Lymphocyte, COVID-19, EBV DNA, Lymphocyte subpopulation, Aged, Aged, 80 and over, CD8-Positive T-Lymphocytes, DNA, Viral, Female, Humans, Intensive Care Units, Killer Cells, Natural, Lymphocyte Count, Lymphocyte Subsets, Middle Aged, Opportunistic Infections, Real-Time Polymerase Chain Reaction, SARS-CoV-2, Viral Load, law.invention, 0302 clinical medicine, law, 80 and over, Killer Cells, Cytotoxic T cell, Viral, 030212 general & internal medicine, biology, musculoskeletal, neural, and ocular physiology, General Medicine, Intensive care unit, Infectious Diseases, medicine.anatomical_structure, Natural, Human herpesvirus 6, Human, Microbiology (medical), 030106 microbiology, macromolecular substances, Article, Virus, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, medicine, lcsh:RC109-216, Parvovirus, business.industry, Herpesvirus 4, DNA, biology.organism_classification, medicine.disease, nervous system, Immunology, business, CD8
Abstract

Highlights • EBV reactivation was observed in patients with COVID-19. • NK + and CD8 + T cell reduction was related to the severity of COVID-19 infection. • An increase of B cells in patients with severe symptoms was documented., Objectives The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. Methods A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Results Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p

Published
2021

12. Characteristics and outcomes of vaccinated and nonvaccinated patients hospitalized in a single Italian hub for COVID-19 during the Delta and Omicron waves in Northern Italy

Author

Rovida, Francesca, primary, Esposito, Giuliana Lucia, additional, Rissone, Marco, additional, Novelli, Viola, additional, Cutti, Sara, additional, Muzzi, Alba, additional, Rona, Claudia, additional, Bertoli, Emanuela, additional, Daglio, Marinella, additional, Piralla, Antonio, additional, Paolucci, Stefania, additional, Campanini, Giulia, additional, Ferrari, Guglielmo, additional, Giardina, Federica, additional, Zavaglio, Federica, additional, Lilleri, Daniele, additional, Grugnetti, Anna Maria, additional, Grugnetti, Giuseppina, additional, Odone, Anna, additional, Marena, Carlo, additional, and Baldanti, Fausto, additional

Published
2022
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15. Comparative analysis of SARS-CoV-2 quasispecies in the upper and lower respiratory tract shows an ongoing evolution in the spike cleavage site

Author

Gaiarsa, Stefano, primary, Giardina, Federica, additional, Batisti Biffignandi, Gherard, additional, Ferrari, Guglielmo, additional, Piazza, Aurora, additional, Tallarita, Monica, additional, Novazzi, Federica, additional, Bandi, Claudio, additional, Paolucci, Stefania, additional, Rovida, Francesca, additional, Campanini, Giulia, additional, Piralla, Antonio, additional, and Baldanti, Fausto, additional

Published
2022
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17. Hydrogen in chondrites: Influence of parent body alteration and atmospheric contamination on primordial components

Author

Dorian Thomassin, Lionel G. Vacher, Thomas Rigaudier, Guillaume Florin, Laurette Piani, Yves Marrocchi, and Maxime Piralla

Subjects
010504 meteorology & atmospheric sciences, Hydrogen, Chemistry, chemistry.chemical_element, Contamination, 010502 geochemistry & geophysics, 01 natural sciences, Parent body, Petrography, Meteorite, 13. Climate action, Geochemistry and Petrology, Chondrite, Environmental chemistry, Earth (classical element), 0105 earth and related environmental sciences, Ordinary chondrite
Abstract

Hydrogen occurs at the near percent level in the most hydrated chondrites (CI and CM) attesting to the presence of water in the asteroid-forming regions. Their H abundances and isotopic signatures are powerful proxies for deciphering the distribution of H in the protoplanetary disk and the origin of Earth's water. Here, we report H contents and isotopic compositions for a set of carbonaceous and ordinary chondrites, including previously analyzed and new samples analyzed after the powdered samples were degassed under vacuum at 120 °C for 48 hours to remove adsorbed atmospheric water. By comparing our results to literature data, we reveal that the H budgets of both H-poor and H-rich carbonaceous chondrites are largely affected by atmospheric moisture, and that their precise quantification requires a specific pre-degassing procedure to correct for terrestrial contamination. Our results show that indigenous H contents of CI carbonaceous chondrites usually considered the most hydrated meteorites might be almost a factor of two lower than those previously reported, with uncontaminated D/H ratios differing significantly from that of Earth's oceans. Without pre-degassing, the H concentrations of H-poor samples (e.g., CVs chondrites) are also affected by terrestrial contamination. After correction for contamination, it appears that the amount of water in chondrites is not controlled by the matrix modal abundance, suggesting that the different chondritic parent bodies accreted variable amounts of water-ice grains. Our results also imply that (i) thermal metamorphism play an important role in determining the H content of both CV and ordinary chondrites but without affecting drastically their H isotopic composition since no clear D enrichment is observed with the increase of petrographic type and (ii) the D enrichment of ordinary chondrite organics does not result from the loss of isotopically light H2 induced by metal oxidation but is rather linked to the persistence of a thermally resistant D-rich component.

Published
2020

18. Human parechovirus type 5 neurological infection in a neonate with a favourable outcome: A case report

Author

S. Perniciaro, Federica Giardina, Agnese De Carli, Massimo Agosti, Serena Ossola, Antonio Piralla, Angela Bossi, and Fausto Baldanti

Subjects
Male, 0301 basic medicine, Microbiology (medical), Pediatrics, medicine.medical_specialty, 030106 microbiology, Breastfeeding, Parechovirus, CSF, Diseases, Neurological examination, Irritability, Infant, Newborn, Diseases, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, CNS infection, Human parechovirus type 5, Molecular characterization, Neonatal infections, Phylogenetic analysis, Humans, Infant, Infant, Newborn, Italy, Nervous System Diseases, Phylogeny, Picornaviridae Infections, medicine, lcsh:RC109-216, 030212 general & internal medicine, biology, medicine.diagnostic_test, business.industry, Crying, Human parechovirus, Meningoencephalitis, General Medicine, Newborn, biology.organism_classification, medicine.disease, Infectious Diseases, Etiology, medicine.symptom, business
Abstract

The majority of parechovirus A type 5 (PeV-A5) infections have been reported in patients with gastrointestinal syndromes. In contrast, a sepsis-like illness associated with PeV-A5 infection has been reported only anecdotally. Herein, we report the first case in Italy of a PeV-A5 neurological infection presenting in a neonate with a sepsis-like syndrome. The patient, a healthy male infant born at 41 weeks of gestation, was highly distressed and inconsolable, and had been crying persistently, with poor breastfeeding, since the previous day. From day 2 to day 4, the newborn was feverish with mild irritability; breastfeeding was preserved and regularly supported. His clinical condition progressively improved, with defervescence on day 4. He was discharged after 7 days, and neurological examination results indicated only mild impairment in visual fixation and vertical eye tracking and mild axial hypotonia. The Italian PeV-A5 strain was phylogenetically related to three strains detected in Denmark in 2012, as well as to one detected in Australia and one in Greece in 2015, with an average nucleotide identity of 97.9% (range 95.9–100.0%). Enterovirus/PeV infection in the newborn should be ruled out in cases of infants with unexplained fever and/or a sepsis-like syndrome and/or meningoencephalitis. An aetiological diagnosis is essential to avoid the unnecessary administration of antibiotics and to plan long-term follow-up until schooling. Keywords: Neonatal infections, Human parechovirus type 5, CSF, Molecular characterization, CNS infection, Phylogenetic analysis

Published
2019

19. Immunity to SARS-CoV-2 up to 15 months after infection

Author

Marcotte, Harold, primary, Piralla, Antonio, additional, Zuo, Fanglei, additional, Du, Likun, additional, Cassaniti, Irene, additional, Wan, Hui, additional, Kumagai-Braesh, Makiko, additional, Andréll, Juni, additional, Percivalle, Elena, additional, Sammartino, Josè Camilla, additional, Wang, Yating, additional, Vlachiotis, Stelios, additional, Attevall, Janine, additional, Bergami, Federica, additional, Ferrari, Alessandro, additional, Colaneri, Marta, additional, Vecchia, Marco, additional, Sambo, Margherita, additional, Zuccaro, Valentina, additional, Asperges, Erika, additional, Bruno, Raffaele, additional, Oggionni, Tiberio, additional, Meloni, Federica, additional, Abolhassani, Hassan, additional, Bertoglio, Federico, additional, Schubert, Maren, additional, Calzolai, Luigi, additional, Varani, Luca, additional, Hust, Michael, additional, Xue, Yintong, additional, Hammarström, Lennart, additional, Baldanti, Fausto, additional, and Pan-Hammarström, Qiang, additional

Published
2022
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20. Humoral and cell-mediated response against SARS-CoV-2 variants elicited by mRNA vaccine BNT162b2 in healthcare workers: a longitudinal observational study

Author

Cassaniti, Irene, primary, Bergami, Federica, additional, Percivalle, Elena, additional, Gabanti, Elisa, additional, Sammartino, Josè Camilla, additional, Ferrari, Alessandro, additional, Adzasehoun, Kodjo Messan Guy, additional, Zavaglio, Federica, additional, Zelini, Paola, additional, Comolli, Giuditta, additional, Sarasini, Antonella, additional, Piralla, Antonio, additional, Ricciardi, Alessandra, additional, Zuccaro, Valentina, additional, Maggi, Fabrizio, additional, Novazzi, Federica, additional, Simonelli, Luca, additional, Varani, Luca, additional, Lilleri, Daniele, additional, and Baldanti, Fausto, additional

Published
2022
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21. EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

Author

Paolucci, Stefania, primary, Cassaniti, Irene, additional, Novazzi, Federica, additional, Fiorina, Loretta, additional, Piralla, Antonio, additional, Comolli, Giuditta, additional, Bruno, Raffaele, additional, Maserati, Renato, additional, Gulminetti, Roberto, additional, Novati, Stefano, additional, Mojoli, Francesco, additional, Baldanti, Fausto, additional, Bruno, R, additional, Mondelli, M, additional, Brunetti, E, additional, Di Matteo, A, additional, Seminari, E, additional, Maiocchi, L, additional, Zuccaro, V, additional, Pagnucco, L, additional, Mariani, B, additional, Ludovisi, S, additional, Lissandrin, R, additional, Parisi, A, additional, Sacchi, P, additional, Patruno, SFA, additional, Michelone, G, additional, Gulminetti, R, additional, Zanaboni, D, additional, Novati, S, additional, Maserati, R, additional, Orsolini, P, additional, Vecchia, M, additional, Sciarra, M, additional, Asperges, E, additional, Colaneri, M, additional, Di Filippo, A, additional, Sambo, M, additional, Biscarini, S, additional, Lupi, M, additional, Roda, S, additional, Pieri, TC, additional, Gallazzi, I, additional, Sachs, M, additional, Valsecchi, P, additional, Perlini, S, additional, Alfano, C, additional, Bonzano, M, additional, Briganti, F, additional, Crescenzi, G, additional, Falchi, AG, additional, Guarnone, R, additional, Guglielmana, B, additional, Maggi, E, additional, Martino, I, additional, Pettenazza, P, additional, Pioli di Marco, S, additional, Quaglia, F, additional, Sabena, A, additional, Salinaro, F, additional, Speciale, F, additional, Zunino, I, additional, De Lorenzo, M, additional, Secco, G, additional, Dimitry, L, additional, Cappa, G, additional, Maisak, I, additional, Chiodi, B, additional, Sciarrini, M, additional, Barcella, B, additional, Resta, F, additional, Moroni, L, additional, Vezzoni, G, additional, Scattaglia, L, additional, Boscolo, E, additional, Zattera, C, additional, Tassi, MF, additional, Capozza, V, additional, Vignaroli, D, additional, Bazzini, M, additional, Iotti, G, additional, Mojoli, F, additional, Belliato, M, additional, Perotti, L, additional, Mongodi, S, additional, Tavazzi, G, additional, Marseglia, G, additional, Licari, A, additional, Brambilla, I, additional, Barbarini, D, additional, Bruno, A, additional, Cambieri, P, additional, Campanini, G, additional, Cavanna, C., additional, Comolli, G, additional, Corbella, M, additional, Daturi, R, additional, Furione, M, additional, Marone, P, additional, Monzillo, E, additional, Paolucci, S, additional, Parea, M, additional, Percivalle, E, additional, Piralla, A, additional, Rovida, F, additional, Sarasini, A, additional, Zavattoni, M, additional, Adzasehoun, G, additional, Ardizzone, M, additional, Bellotti, L, additional, Brunco, V, additional, Cabano, E, additional, Casali, G, additional, Capella, L, additional, Devitis, D, additional, Dossena, L, additional, Frisco, G, additional, Garbagnoli, G, additional, Gardellini, F, additional, Girello, A, additional, Guerrizio, A, additional, Landini, V, additional, Lucchelli, C, additional, Maliardi, V, additional, Piemontese, P, additional, Pezzaia, S, additional, Premoli, M, additional, Rebuffa, C, additional, Zanello, C, additional, Bagnarino, J, additional, Bergami, F, additional, Bonetti, A, additional, Caneva, G, additional, Cassaniti, I, additional, Corcione, A, additional, Di Martino, R, additional, Di Napoli, A, additional, Ferrari, A, additional, Ferrari, G, additional, Fiorina, L, additional, Gallone, A, additional, Giardina, F, additional, Girardi, A, additional, Mercato, A, additional, Merla, C, additional, Novazzi, F, additional, Ratano, G, additional, Rossi, B, additional, Saveriaempillai, G, additional, Sciabica, IM, additional, Tallarita, M, additional, Vecchio Nepita, E, additional, Vitali, J, additional, Cerino, A, additional, Varchetta, S, additional, Oliviero, B, additional, Mantovani, S, additional, Mele, D, additional, Calvi, M, additional, Tizzoni, M, additional, Nicora, C, additional, Triarico, A, additional, Petronella, V, additional, Marena, C, additional, Muzzi, A, additional, Lago, P, additional, Cutti, S, additional, Novelli, V, additional, Comandatore, F, additional, BatistiBiffignandi, G, additional, Gaiarsa, S, additional, Rettani, M, additional, and Bandi, C, additional

Published
2021
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23. Cytomegalovirus and Epstein-Barr Virus DNA Kinetics in Whole Blood and Plasma of Allogeneic Hematopoietic Stem Cell Transplantation Recipients

Author

Lazzarotto T, Chiereghin A, Piralla A, Piccirilli G, Girello A, Campanini G, Gabrielli L, Costa C, Prete A, Bonifazi F, Busca A, Cairoli R, Colombo AA, Zecca M, Sidoti F, Bianco G, Paba P, Perno CF, Cavallo R, Baldanti F, AMCLI-GLaIT working group., Lazzarotto T, Chiereghin A, Piralla A, Piccirilli G, Girello A, Campanini G, Gabrielli L, Costa C, Prete A, Bonifazi F, Busca A, Cairoli R, Colombo AA, Zecca M, Sidoti F, Bianco G, Paba P, Perno CF, Cavallo R, Baldanti F, AMCLI-GLaIT working group., Tiziana, L, Angela, C, Antonio, P, Giulia, P, Alessia, G, Giulia, C, Liliana, G, Cristina, C, Arcangelo, P, Francesca, B, Alessandro, B, Cairoli, R, Anna Amelia, C, Marco, Z, Francesca, S, Gabriele, B, Pierpaolo, P, Carlo Federico, P, Rossana, C, and Fausto, B

Subjects
Adult, Male, 0301 basic medicine, Herpesvirus 4, Human, medicine.medical_specialty, medicine.medical_treatment, 030106 microbiology, Congenital cytomegalovirus infection, Allogeneic hematopoietic stem cell transplantation, CMV and EBV DNA kinetics, Plasma, Whole blood, Hematology, Transplantation, Cytomegalovirus, Hematopoietic stem cell transplantation, Virus Replication, Virus, 03 medical and health sciences, chemistry.chemical_compound, Internal medicine, medicine, Humans, Aged, Retrospective Studies, CMV and EBV DNA kinetic, business.industry, Hematopoietic Stem Cell Transplantation, virus diseases, Middle Aged, Allografts, medicine.disease, Virology, Transplant Recipients, Kinetics, Blood, 030104 developmental biology, chemistry, Viral replication, DNA, Viral, Female, business, DNA
Abstract

Currently, no consensus has been reached on the optimal blood compartment to be used for surveillance of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) DNAemia. Although several comparative studies have been performed correlating CMV and EBV DNA loads in whole blood (WB) versus plasma, to our knowledge, no studies to date have analyzed the kinetics of both viruses in the 2 blood compartments. In this retrospective noninterventional multicenter cohort study, the kinetics of CMV and EBV DNA in 121 hematopoietic stem cell transplantation (HSCT) recipients were investigated by analyzing in parallel 569 and 351 paired samples from 80 and 58 sequential episodes of CMV and EBV DNAemia, respectively. Unlike previous studies, this study used a single automated molecular method that was CE-marked and Food and Drug Administration-approved for use in quantifying CMV and EBV DNA in both plasma and WB. Furthermore, the complete viral replication kinetics of all episodes (including both the ascending and the descending phases of the active infection) was examined in each patient. The previously observed overall correlation between CMV DNA levels in WB and plasma was confirmed (Spearman's ρ =.85; P

Published
2018

25. Looking for fever in nursing home residents with COVID-19: A false friend?

Author

Antonio Piralla, Fausto Baldanti, Gianluigi Poma, Ambra Raimondi, Carlo Filice, and Valeria Meroni

Subjects
0301 basic medicine, Male, Microbiology (medical), medicine.medical_specialty, 2019-20 coronavirus outbreak, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 030106 microbiology, Population, nursing homes, Real-Time Polymerase Chain Reaction, elderly, Serology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Elderly population, medicine, Humans, lcsh:RC109-216, 030212 general & internal medicine, education, Aged, Aged, 80 and over, fever, education.field_of_study, business.industry, SARS-CoV-2, Serological assay, COVID-19, General Medicine, Infectious Diseases, Perspective, Female, Nursing homes, business
Abstract

Highlights • Elderly patients in nursing homes often present an atypical course of COVID-19 disease. • Fever is one of the pivotal signs in COVID-19 patients. • The prevalence of fever was extremely low in our population of nursing home SARS-CoV-2-positive patients. • This finding must be considered in the diagnostic workup of suspected COVID-19 patients., Objectives Residents in nursing homes represent a frail population of patients, and SARS-CoV-2 infection can spread easily in this setting. Despite a frequent severe evolution of the disease, these patients often present an atypical course with mild initial symptoms. Thus, the aim of this study was to assess the occurrence of fever in elderly SARS-CoV-2 positive patients residing in nursing homes. Methods 231 elderly patients from three nursing homes of Pavia and its Province were enrolled in April-May 2020. Diagnosis of SARS-CoV-2 infection was performed using real-time RT-PCR on nasopharyngeal swab and/or serological assay (LIAISON® SARS-CoV-2 S1/S2 IgG). Positive patients were defined by positivity at nasopharyngeal swab and/or at serology. Results 170 patients (74%) were SARS-CoV-2-positive, 61 subjects (26%) resulted negative at both tests. Fever (T > 37,5 °C) was observed only in four patients (1,7%), three in the SARS-CoV-2-positive group (1,7%) and one in the SARS-CoV-2-negative one (1,6%). Conclusions The prevalence of fever was extremely low in our population of nursing home SARS-CoV-2-positive patients. Thus, this finding must be taken into consideration when screening patients without fever in nursing homes.

Published
2021
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26. SARS-CoV-2 specific T-cell immunity in COVID-19 convalescent patients and unexposed controls measured by ex vivo ELISpot assay

Author

Cassaniti, Irene, primary, Percivalle, Elena, additional, Bergami, Federica, additional, Piralla, Antonio, additional, Comolli, Giuditta, additional, Bruno, Raffaele, additional, Vecchia, Marco, additional, Sambo, Margherita, additional, Colaneri, Marta, additional, Zuccaro, Valentina, additional, Benazzo, Marco, additional, Robotti, Carlo, additional, Calastri, Anna, additional, Maiorano, Eugenia, additional, Ferrari, Alessandro, additional, Cambiè, Giuseppe, additional, and Baldanti, Fausto, additional

Published
2021
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29. Persistence of SARS-CoV-2-specific B and T cell responses in convalescent COVID-19 patients 6–8 months after the infection

Author

Sherina, Natalia, primary, Piralla, Antonio, additional, Du, Likun, additional, Wan, Hui, additional, Kumagai-Braesch, Makiko, additional, Andréll, Juni, additional, Braesch-Andersen, Sten, additional, Cassaniti, Irene, additional, Percivalle, Elena, additional, Sarasini, Antonella, additional, Bergami, Federica, additional, Di Martino, Raffaella, additional, Colaneri, Marta, additional, Vecchia, Marco, additional, Sambo, Margherita, additional, Zuccaro, Valentina, additional, Bruno, Raffaele, additional, Sachs, Michele, additional, Oggionni, Tiberio, additional, Meloni, Federica, additional, Abolhassani, Hassan, additional, Bertoglio, Federico, additional, Schubert, Maren, additional, Byrne-Steele, Miranda, additional, Han, Jian, additional, Hust, Michael, additional, Xue, Yintong, additional, Hammarström, Lennart, additional, Baldanti, Fausto, additional, Marcotte, Harold, additional, and Pan-Hammarström, Qiang, additional

Published
2021
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30. SARS-CoV-2 positivity in rectal swabs: implication for possible transmission

Author

Raffaele Bruno, Fausto Baldanti, Federica Novazzi, Antonio Di Sabatino, Antonio Piralla, and Irene Cassaniti

Subjects
Microbiology (medical), 2019-20 coronavirus outbreak, biology, Coronavirus disease 2019 (COVID-19), Transmission (medicine), business.industry, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Immunology, medicine.disease_cause, biology.organism_classification, Virology, Microbiology, QR1-502, Article, Medicine, Immunology and Allergy, business, Betacoronavirus, Coronavirus Infections, Coronavirus
Published
2020
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31. Severe acute respiratory syndrome coronavirus 2 RNA contamination of inanimate surfaces and virus viability in a health care emergency unit

Author

Colaneri, M., Seminari, E., Novati, S., Asperges, E., Biscarini, S., Piralla, A., Percivalle, E., Cassaniti, I., Baldanti, F., Bruno, R., Mondelli, M. U., Brunetti, E., Di Matteo, A., Maiocchi, L., Zuccaro, V., Pagnucco, L., Ludovisi, S., Lissandrin, R., Parisi, A., Sacchi, P., Patruno, S. F. A., Michelone, G., Gulminetti, R., Zanaboni, D., Maserati, R., Orsolini, P., Vecchia, M., Di Filippo, A., Sambo, M., Lupi, M., Roda, S., Pieri, T. C., Gallazzi, I., Sachs, M., Valsecchi, P., Perlini, S., Alfano, C., Bonzano, M., Briganti, F., Crescenzi, G., Falchi, A. G., Guarnone, R., Guglielmana, B., Maggi, E., Martino, I., Pettenazza, P., di Marco, S. P., Quaglia, F., Sabena, A., Salinaro, F., Speciale, F., Zunino, I., de Lorenzo, M., Secco, G., Dimitry, L., Cappa, G., Maisak, I., Chiodi, B., Sciarrini, M., Barcella, B., Resta, F., Moroni, L., Vezzoni, G., Scattaglia, L., Boscolo, E., Zattera, C., Fidel, T. M., Vincenzo, C., Vignaroli, D., Bazzini, M., Iotti, G., Mojoli, F., Belliato, M., Perotti, L., Mongodi, S., Tavazzi, G., Marseglia, G., Licari, A., Brambilla, I., Barbarini, D., Bruno, A., Cambieri, P., Campanini, G., Cavanna, C., Comolli, G., Corbella, M., Daturi, R., Furione, M., Mariani, B., Marone, P., Monzillo, V., Paolucci, S., Parea, M., Rovida, F., Sarasini, A., Zavattoni, M., Adzasehoun, G., Ardizzone, M., Bellotti, L., Brunco, V., Cabano, E., Casali, G., Capella, L., Devitis, D., Dossena, L., Frisco, G., Garbagnoli, G., Gardellini, F., Girello, A., Guerrizio, A., Landini, V., Lucchelli, C., Maliardi, V., Piemontese, P., Pezzaia, S., Premoli, M., Rebuffa, C., Bagnarino, J., Bergami, F., Bonetti, A., Caneva, G., Corcione, A., Di Martino, R., Di Napoli, A., Ferrari, A., Ferrari, G., Fiorina, L., Gallone, A., Giardina, F., Girardi, A., Mercato, A., Novazzi, F., Ratano, G., Rossi, B., Saverimpilla, G., Sciabica, I. M., Tallarita, M., Nepita, E. V., Vitali, J., Cerino, A., Varchetta, S., Oliviero, B., Mantovani, S., Mele, D., Calvi, M., Tizzoni, M., Nicola, C., Triarico, A., Petronella, V., Marena, C., Muzzi, A., Lago, P., Comandatore, F., Biffignandi, G. B., Gaiarsa, S., Rettani, M., and Bandi, C.

Subjects
0301 basic medicine, Microbiology (medical), Environmental contamination, medicine.medical_treatment, 030106 microbiology, Microbiology, Coronavirus disease 2019, Severe acute respiratory syndrome coronavirus-2, Surfaces, Vero E6 cells, Animals, Betacoronavirus, Chlorocebus aethiops, Coronavirus Envelope Proteins, Equipment Contamination, Fomites, Humans, Intensive Care Units, Microbial Viability, RNA-Dependent RNA Polymerase, Reverse Transcriptase Polymerase Chain Reaction, SARS-CoV-2, Vero Cells, Viral Envelope Proteins, Viral Proteins, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, RNA polymerase, medicine, 030212 general & internal medicine, Continuous positive airway pressure, Respiratory system, Gene, Cytopathic effect, business.industry, RNA, General Medicine, Infectious Diseases, chemistry, Vero cell, business
Abstract

Objectives To detect possible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA contamination of inanimate surfaces in areas at high risk of aerosol formation by patients with coronavirus disease 2019 (COVID-19). Methods Sampling was performed in the emergency unit and the sub-intensive care ward. SARS-CoV-2 RNA was extracted from swabbed surfaces and objects and subjected to real-time RT-PCR targeting RNA-dependent RNA polymerase and E genes. Virus isolation from positive samples was attempted in vitro on Vero E6 cells. Results Twenty-six samples were collected and only two were positive for low-level SARS-CoV-2 RNA, both collected on the external surface of continuous positive airway pressure helmets. All transport media were inoculated onto susceptible cells, but none induced a cytopathic effect on day 7 of culture. Conclusions Even though daily contact with inanimate surfaces and patient fomites in contaminated areas may be a medium of infection, our data obtained in real-life conditions suggest that it might be less extensive than hitherto recognized.

Published
2020
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32. Rapid and sensitive detection of SARS-CoV-2 RNA using the Simplexa™ COVID-19 direct assay

Author

Fausto Baldanti, Federica Novazzi, Silvia Meschi, Giulia Minnucci, Giuseppe Sberna, Eleonora Lalle, Monica Tallarita, Licia Bordi, Veronica Tettamanzi, Concetta Castilletti, Angela Brisci, Maria Rosaria Capobianchi, Antonio Piralla, Francesca Colavita, and Federica Giardina

Subjects
0301 basic medicine, Viral nucleic acid, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 030106 microbiology, Pneumonia, Viral, Sensitivity and Specificity, Article, 03 medical and health sciences, Betacoronavirus, 0302 clinical medicine, Diagnostic Tests, Limit of Detection, Virology, Rapid response, Real-time RT-PCR, SARS-CoV-2, Surveillance, Clinical Laboratory Techniques, Coronavirus Infections, Routine, Humans, Pandemics, Pneumonia, Viral, RNA, Medicine, Viral rna, 030212 general & internal medicine, Detection limit, Concordance analysis, business.industry, Diagnostic Tests, Routine, COVID-19, Molecular biology, 3. Good health, Infectious Diseases, Direct assay, RNA, Viral, business
Abstract

Highlights • High sensitivity of Simplexa™ COVID-19 Direct assay. • 100 % specificity of this new assay. • “Almost perfect” agreement with Corman’s method (κ = 0.938). • High-speed detection, all-in-one reagent mix and no separate extraction required. • Promising for laboratory diagnosis of COVID-19 and field application., Background So far, one of the major drawbacks of the available molecular assays for the diagnosis of severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) is the need for viral nucleic acid extraction from clinical specimens. Objective The aim of this study was to evaluate the performances of a newly designed real-time RT-PCR (Simplexa™ COVID-19 Direct assay), that is established with an all-in-one reagent mix and no separate extraction required. Results The lower limit of detection (LOD) for both target genes resulted the same: 3.2 (CI: 2.9–3.8) log10 cp/mL and 0.40 (CI: 0.2–1.5) TCID50/mL for S gene while 3.2 log10 (CI: 2.9–3.7) log10 cp/mL and 0.4 (CI: 0.2–1.3) TCID50/mL for ORF1ab. The LOD obtained with extracted viral RNA for both S gene or ORF1ab was 2.7 log10 cp/mL. Crossreactive analysis performed in 20 nasopharyngeal swabs confirmed a 100% of clinical specificity of the assay. Clinical performances of Simplexa™ COVID-19 Direct assay were assessed in 278 nasopharyngeal swabs tested in parallel with Corman's method. Concordance analysis showed an "almost perfect" agreement in SARS-CoV-2 RNA detection between the two assays, being κ = 0.938; SE = 0.021; 95% CI = 0.896-0.980. Conclusions The high sensitivity and specificity of this new assay indicate that it is promising for laboratory diagnosis, enabling highspeed detection in just over one hour, which is significantly faster than the up to five hours currently required by traditional extraction followed by amplification technologies, thus allowing prompt decision making regarding isolation of infected patients.

Published
2020
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33. Enterovirus-D68 (EV-D68) in pediatric patients with respiratory infection: The circulation of a new B3 clade in Italy

Author

Susanna Esposito, Silvia Caimmi, Giovanna Lunghi, Federica Giardina, Gian Luigi Marseglia, Luca Ruggiero, Elisabetta De Sando, Sonia Bianchini, Alessia Girello, Nicola Principi, Fausto Baldanti, and Antonio Piralla

Subjects
Male, 0301 basic medicine, medicine.medical_specialty, Adolescent, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Article, 03 medical and health sciences, EV-D68, Nasopharynx, Respiratory infection, Virology, Internal medicine, Enterovirus Infections, medicine, Humans, Child, Respiratory Tract Infections, Phylogeny, Retrospective Studies, Enterovirus D, Human, Molecular Epidemiology, Molecular epidemiology, business.industry, Infant, Outbreak, Subclade, Retrospective cohort study, Sequence Analysis, DNA, Pneumonia, medicine.disease, Pediatric infectious disease, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Enterovirus-D68, Italy, Child, Preschool, Mutation, Enterovirus, Capsid Proteins, Female, Emergency Service, Hospital, business, Respiratory tract
Abstract

Highlights • Human enterovirus D68 (EV-D68) is a common cause of lower respiratory illness in pediatric age. • In most of the cases, EV-D68 is detected as a single infectious agent. • The circulation of EV-D68 B3 lineage has been proven in Italy during 2016. • The continuous global monitoring of epidemiology of EV-D68 should be improved., Background In recent years, several outbreaks due to Enterovirus D-68 (EV-D68) have been reported, and it was confirmed that the virus can cause upper and lower respiratory tract diseases and be associated with the development of neurological problems. Objectives The main aim of this research was to study the genetic characteristics of EV-D68 strains that were circulating in Italy identified during an outbreak of an EV-D68 infection that occurred in Italy during the period March-October 2016. Study design A retrospective study of the circulation of different types and subtypes of EV-D68 was performed. Nasopharyngeal swabs were collected from March 2016 through October 2016 in children admitted to the Emergency Room with respiratory diseases. Results Among 390 children, 22 (59.1% males; mean age 47 months) were found to be infected by EV-D68 and most of them were immunocompetent (72.7%). Pneumonia was diagnosed in 12 (54.5%) children. Phylogenetic analysis of the VP1 region showed that all the strains identified in this study belonged to clade B3. Within B3 subclade, the Italian EV-D68 strains were most closely related to strains detected in Southern China in 2015 as well as to strains detected in US and the Netherlands in 2016. Conclusions These results showed that EV-D68 infections are a common cause of lower respiratory illness in pediatric age. The circulation of one EV-D68 lineage has been proven in Italy and in the European region during 2016. However, further studies are required to investigate whether some strains or lineages may possess a higher affinity for the lower airway or central nervous system.

Published
2018

34. Outbreak of measles genotype H1 in Northern Italy originated from a case imported from Southeast Asia, 2017

Author

Fausto Baldanti, Melissa Baggieri, Chiara Fornabaio, A. Piro, Irene Cassaniti, A. Monteverdi, Fabio Magurano, Francesca Rovida, Angelo Pan, A. Sarasini, N. Brianese, Alessia Girello, Antonio Piralla, Elena Seminari, Federica Giardina, and Danilo Cereda

Subjects
Adult, Microbiology (medical), Genotype, medicine.medical_treatment, Measles, Disease Outbreaks, Measles virus, Communicable Diseases, Imported, Maculopapular rash, medicine, Cluster Analysis, Humans, Infection control, Post-exposure prophylaxis, Asia, Southeastern, Infection Control, biology, Outbreak, General Medicine, Middle Aged, biology.organism_classification, medicine.disease, Virology, Diarrhea, Infectious Diseases, Geography, Italy, medicine.symptom
Published
2019

35. Residual SARS-CoV-2 RNA in nasal swabs of convalescent COVID-19 patients: Is prolonged quarantine always justified?

Author

Piralla, Antonio, primary, Ricchi, Matteo, additional, Cusi, Maria Grazia, additional, Prati, Paola, additional, Vicari, Nadia, additional, Scarsi, Giada, additional, Gandolfo, Claudia, additional, Anichini, Gabriele, additional, Terrosi, Chiara, additional, Percivalle, Elena, additional, Vecchio Nepita, Edoardo, additional, Bergami, Federica, additional, Tallarita, Monica, additional, Di Martino, Raffaella, additional, Ferrari, Alessandro, additional, Rovida, Francesca, additional, Lunghi, Giovanna, additional, Schiavo, Roberta, additional, and Baldanti, Fausto, additional

Published
2021
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37. Low risk for SARS-CoV2 symptomatic infection and early complications in paediatric patients during the ongoing CoVID19 epidemics in Lombardy

Author

Rovida, F., primary, Cereda, D., additional, Novati, S., additional, Licari, A., additional, Triarico, A., additional, Marseglia, G.L., additional, Bruno, R., additional, Baldanti, F., additional, Mondelli, M., additional, Brunetti, E., additional, Di Matteo, A., additional, Seminari, E., additional, Maiocchi, L., additional, Zuccaro, V., additional, Pagnucco, L., additional, Mariani, B., additional, Ludovisi, S., additional, Lissandrin, R., additional, Parisi, A., additional, Sacchi, P., additional, Patruno, S.F.A., additional, Michelone, G., additional, Gulminetti, R., additional, Zanaboni, D., additional, Maserati, R., additional, Orsolini, P., additional, Vecchia, M., additional, Sciarra, M., additional, Asperges, E., additional, Colaneri, M., additional, Di Filippo, A., additional, Sambo, M., additional, Biscarini, S., additional, Lupi, M., additional, Roda, S., additional, Pieri, T.C., additional, Gallazzi, I., additional, Sachs, M., additional, Valsecchi, P., additional, Perlini, S., additional, Alfano, C., additional, Bonzano, M., additional, Briganti, F., additional, Crescenzi, G., additional, Falchi, A.G., additional, Guarnone, R., additional, Guglielmana, B., additional, Maggi, E., additional, Martino, I., additional, Pettenazza, P., additional, Pioli di Marco, S., additional, Quaglia, F., additional, Sabena, A., additional, Salinaro, F., additional, Speciale, F., additional, Zunino, I., additional, De Lorenzo, M., additional, Secco, G., additional, Dimitry, L., additional, Cappa, G., additional, Maisak, I., additional, Chiodi, B., additional, Sciarrini, M., additional, Barcella, B., additional, Resta, F., additional, Moroni, L., additional, Vezzoni, G., additional, Scattaglia, L., additional, Boscolo, E., additional, Zattera, C., additional, Tassi, M.F., additional, Capozza, V., additional, Vignaroli, D., additional, Bazzini, M., additional, Iotti, G., additional, Mojoli, F., additional, Belliato, M., additional, Perotti, L., additional, Mongodi, S., additional, Tavazzi, G., additional, Marseglia, G., additional, Brambilla, I., additional, Barbarini, D., additional, Bruno, A., additional, Cambieri, P., additional, Campanini, G., additional, Comolli, G., additional, Corbella, M., additional, Daturi, R., additional, Furione, M., additional, Monzillo, E., additional, Paolucci, S., additional, Parea, M., additional, Percivalle, E., additional, Piralla, A., additional, Rovida, F., additional, Sarasini, A., additional, Zavattoni, M., additional, Adzasehoun, G., additional, Bellotti, L., additional, Cabano, E., additional, Casali, G., additional, Dossena, L., additional, Frisco, G., additional, Garbagnoli, G., additional, Girello, A., additional, Landini, V., additional, Lucchelli, C., additional, Maliardi, V., additional, Pezzaia, S., additional, Premoli, M., additional, Bonetti, A., additional, Caneva, G., additional, Cassaniti, I., additional, Corcione, A., additional, Di Martino, R., additional, Di Napoli, A., additional, Ferrari, A., additional, Ferrari, G., additional, Fiorina, L., additional, Giardina, F., additional, Mercato, A., additional, Novazzi, F., additional, Ratano, G., additional, Rossi, B., additional, Sciabica, I.M., additional, Tallarita, M., additional, Vecchio Nepita, E., additional, Calvi, M., additional, Tizzoni, M., additional, Nicora, C., additional, Petronella, V., additional, Marena, C., additional, Muzzi, A., additional, and Lago, P., additional

Published
2020
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39. Rapid and sensitive detection of SARS-CoV-2 RNA using the Simplexa™ COVID-19 direct assay

Author

Bordi, Licia, primary, Piralla, Antonio, additional, Lalle, Eleonora, additional, Giardina, Federica, additional, Colavita, Francesca, additional, Tallarita, Monica, additional, Sberna, Giuseppe, additional, Novazzi, Federica, additional, Meschi, Silvia, additional, Castilletti, Concetta, additional, Brisci, Angela, additional, Minnucci, Giulia, additional, Tettamanzi, Veronica, additional, Baldanti, Fausto, additional, and Capobianchi, Maria Rosaria, additional

Published
2020
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42. Circulation of non-polio enteroviruses in 24 EU and EEA countries between 2015 and 2017: a retrospective surveillance study

Author

Bubba, Laura, primary, Broberg, Eeva K, additional, Jasir, Aftab, additional, Simmonds, Peter, additional, Harvala, Heli, additional, Redlberger-Fritz, Monika, additional, Nikolaeva-Glomb, Lubomira, additional, Havlíčková, Martina, additional, Rainetova, Petra, additional, Fischer, Thea K, additional, Midgley, Sofie E., additional, Epštein, Jevgenia, additional, Blomqvist, Soile, additional, Böttcher, Sindy, additional, Keeren, Kathrin, additional, Bujaki, Erika, additional, Farkas, Ágnes, additional, Baldvinsdóttir, Guôrún Erna, additional, Morley, Ursula, additional, De Gascun, Cillian, additional, Pellegrinelli, Laura, additional, Piralla, Antonio, additional, Martinuka, Oksana, additional, Zamjatina, Natālija, additional, Griškevičius, Algirdas, additional, Nguyen, Trung, additional, Dudman, Susanne G., additional, Numanovic, Sanela, additional, Wieczorek, Magdalena, additional, Guiomar, Raquel, additional, Costa, Inês, additional, Cristina, Tecu, additional, Bopegamage, Shubhada, additional, Pastuchova, Katarina, additional, Berginc, Nataša, additional, Cabrerizo, María, additional, González-Sanz, Rubén, additional, Zakikhany, Katherina, additional, Hauzenberger, Elenor, additional, Benschop, Kimberley, additional, Duizer, Erwin, additional, Dunning, Jake, additional, Celma, Cristina, additional, McKenna, James, additional, Feeney, Susan, additional, Templeton, Kate, additional, Moore, Catherine, additional, and Cottrell, Simon, additional

Published
2020
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45. Persistence of SARS-CoV-2-specific B and T cell responses in convalescent COVID-19 patients 6–8 months after the infection

Author

Raffaella Di Martino, Michele Sachs, Fausto Baldanti, Hassan Abolhassani, Valentina Zuccaro, Marco Vecchia, Lennart Hammarström, Federica Meloni, Antonio Piralla, Federico Bertoglio, Tiberio Oggionni, Michael Hust, Qiang Pan-Hammarström, Margherita Sambo, Harold Marcotte, Yintong Xue, Sten Braesch-Andersen, Federica Bergami, Hui Wan, Marta Colaneri, Jian Han, Irene Cassaniti, Maren Schubert, Miranda Byrne-Steele, Antonella Sarasini, Raffaele Bruno, Makiko Kumagai-Braesch, Natalia Sherina, Elena Percivalle, Likun Du, and Juni Andréll

Subjects
T-Lymphocytes, longevity of immune response, Antibodies, Viral, immunological memory, 03 medical and health sciences, 0302 clinical medicine, Immune system, Immunity, antibody, Humans, Medicine, media_common.cataloged_instance, European union, Neutralizing antibody, 030304 developmental biology, media_common, B cell, 0303 health sciences, biology, SARS-CoV-2, business.industry, COVID-19, T cell, General Medicine, Acquired immune system, immunity, Immunoglobulin A, 3. Good health, Vaccination, Immunoglobulin M, Immunoglobulin G, 030220 oncology & carcinogenesis, Immunology, biology.protein, Clinical and Translational Article, Antibody, business
Abstract

Background Monitoring the adaptive immune responses during the natural course of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection provides useful information for the development of vaccination strategies against this virus and its emerging variants. We thus profiled the serum anti-SARS-CoV-2 antibody levels and specific memory B- and T-cell responses in convalescent coronavirus disease-2019 (COVID-19) patients. Methods Altogether 119 samples from 88 convalescent donors who experienced mild to critical disease were tested for the presence of elevated anti-spike and anti-receptor binding domain antibody levels over a period of eight months. In addition, level of SARS-CoV-2 neutralizing antibodies, specific memory B- and T-cell responses were tested in a subset of samples. Findings Anti-SARS-CoV-2 antibodies were present in 85% of the samples collected within 4 weeks after onset of symptoms in COVID-19 patients. Levels of specific IgM/IgA antibodies declined after 1 month while levels of specific IgG antibodies and plasma neutralizing activities remained relatively stable up to 6 months after diagnosis. Anti-SARS-CoV-2 IgG antibodies were still present, though at a significantly lower level, in 80% of the samples collected at 6-8 months after symptom onset. SARS-CoV-2-specific memory B- and T-cell responses developed with time and were persistent in all patients followed up till 6-8 months. Conclusions Our data suggest that protective adaptive immunity following natural infection of SARS-CoV-2 might persist for at least 6-8 months, regardless of disease severity. Development of medium or long-term protective immunity through vaccination might thus be possible. Funding EU-ATAC consortium, the Italian Ministry of Health and SciLife/KAW., Graphical Abstract, In this study, Sherina et al. showed that although the SARS-CoV-2 specific antibody levels decreased over time, the majority of tested COVID-19 patients developed and maintained virus-specific B- and T-cell memory up to at least 6-8 months following infection, regardless of the disease severity.

Published
2021

46. Acute respiratory distress syndrome in adenovirus type 4 pneumonia: A case report

Author

R. Narra, Giovanna Lunghi, Antonio Piralla, S Crotti, Giacomo Grasselli, Anna Orlandi, Patrizia Bono, S. Piconi, Fausto Baldanti, Alessia Girello, and Antonella Zoccoli

Subjects
Adult, 0301 basic medicine, medicine.medical_specialty, Critical Care, medicine.medical_treatment, 030106 microbiology, Polymerase Chain Reaction, law.invention, Adenovirus Infections, Human, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, law, Virology, Internal medicine, Extracorporeal membrane oxygenation, medicine, Humans, 030212 general & internal medicine, Respiratory system, Intensive care medicine, Mechanical ventilation, Respiratory Distress Syndrome, Leukopenia, Respiratory distress, business.industry, Adenoviruses, Human, medicine.disease, Intensive care unit, Hospitalization, Molecular Typing, Pneumonia, Infectious Diseases, chemistry, DNA, Viral, Female, medicine.symptom, business, Cidofovir
Abstract

Human adenoviruses (HAdVs) cause a wide spectrum of clinical syndromes, depending on species and types, from mild respiratory infections to deadly pneumonia: in particular, severe infections occur in immunocompromised patients. In this report, we describe the case of a 36 years-old woman admitted to our intensive care unit (ICU) with severe respiratory distress syndrome caused by adenovirus pneumonia, that required invasive respiratory support (mechanical ventilation and extracorporeal membrane oxygenation). Molecular assays detected the virus in respiratory and plasma specimen and sequencing procedure identified HAdV type 4. Patient improved after cidofovir administration. Leukopenia and subsequent bacterial infection occurred, but the patient recovered completely and was discharged from the hospital after 54days.

Published
2016

47. Genetic variability of the measles virus hemagglutinin gene in B3 genotype strains circulating in Northern Italy

Author

Ciceri, G., primary, Canuti, M., additional, Bianchi, S., additional, Gori, M., additional, Piralla, A., additional, Colzani, D., additional, Libretti, M., additional, Frati, E.R., additional, Baggieri, M., additional, Lai, A., additional, Rovida, F., additional, Zehender, G., additional, Baldanti, F., additional, Magurano, F., additional, Tanzi, E., additional, and Amendola, A., additional

Published
2019
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48. Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load

Author

Sidoti, Francesca, primary, Piralla, Antonio, additional, Costa, Cristina, additional, Scarasciulli, Maria Luisa, additional, Calvario, Agata, additional, Conaldi, Pier Giulio, additional, Paba, Pierpaolo, additional, Perno, Carlo Federico, additional, Gaeta, Aurelia, additional, Antonelli, Guido, additional, Sodano, Giuseppe, additional, Santangelo, Rosaria, additional, Sanguinetti, Maurizio, additional, Vatteroni, Maria Linda, additional, Barzon, Luisa, additional, Palù, Giorgio, additional, Abbate, Isabella, additional, Capobianchi, Maria Rosaria, additional, Piccirilli, Giulia, additional, Lazzarotto, Tiziana, additional, Baldanti, Fausto, additional, and Cavallo, Rossana, additional

Published
2019
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49. Outbreak of measles genotype H1 in Northern Italy originated from a case imported from Southeast Asia, 2017

Author

Rovida, F., primary, Brianese, N., additional, Piralla, A., additional, Sarasini, A., additional, Girello, A., additional, Giardina, F., additional, Cassaniti, I., additional, Fornabaio, C., additional, Seminari, E., additional, Monteverdi, A., additional, Piro, A., additional, Baggieri, M., additional, Cereda, D., additional, Pan, A., additional, Magurano, F., additional, and Baldanti, F., additional

Published
2019
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