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Thrombocytopenia is common in patients with myelodysplastic syndromes (MDS) and immune destruction of platelets could be an important factor for its occurrence. We prospectively analyzed platelet-associated IgG (PAIgG) through platelet immunofluorescence test (PIFT), mean platelet volume (MPV), platelet size deviation width (PDW) and glycocalicin index (GCI) of 54 patients with MDS, classified according to the International Prognostic Scoring System (IPSS). Thrombocytopenia (platelet count

Anti-platelet antibody has been generally recognized as an important etiologic factor in idiopathic thrombocytopenic purpura (ITP) and the detection of platelet associated IgG (PAIgG) is essential to establish the diagnosis and also useful to predict the clinical course. Several methods to detect PAIgG have been proposed, most of which, however, require complicated manipulation and are not practical for clinical use. This paper describes a simple and rapid method to detect PAIgG.This method was devised, utilizing the ability of staphylococcal protein A (SpA) to bind specifically to human IgG. An adhesive percentage of sample platelets to sheep red blood cells coated with SpA was calculated by enumeration of sample platelet before and after treatment. It takes only several hours to complete the assay from the drawing of blood. In a total of 37 cases including 15 cases of ITP, PAIgG was studied, using this method and Fab, anti-Fab assay of Luiken. Binding Index (an adhesive percentage) was 9.3 ± 3.8% in mormal subjects and 26.5 ± 10% in ITP patients, which differnce was statistically significant (pThese results suggest that this new method is reliable to detect PAIgG in addition to its simplicity and rapidity and practical in clinical use.