1. A c-MWCNTs/AuNPs-based electrochemical cytosensor to evaluate the anticancer activity of pinoresinol from Cinnamomum camphora against HeLa cells
- Author
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Haixu, Zhou, Rengui, Huang, Tongchao, Su, Bo, Li, Haoyu, Zhou, Jiali, Ren, and Zhonghai, Li
- Subjects
Cinnamomum camphora ,Nanotubes, Carbon ,Biophysics ,Metal Nanoparticles ,Biosensing Techniques ,Electrochemical Techniques ,General Medicine ,Lignans ,Nanocomposites ,Limit of Detection ,Electrochemistry ,Humans ,Gold ,Physical and Theoretical Chemistry ,Furans ,Electrodes ,HeLa Cells - Abstract
A sensitive, simple, label-free electrochemical cytosensor was developed to evaluate the anticancer activity of pinoresinol against human cervical carcinoma (HeLa) cells. HeLa cells were immobilised on carboxylated multi-walled carbon nanotubes (c-MWCNTs)/gold nanoparticles (AuNPs) nanocomposite-modified glassy carbon electrodes. Scanning electron microscopy, transmission electron microscopy, Fourier transform-infrared spectroscopy, and X-ray diffractometry were used to characterise the morphology, crystallinity, and composition of the nanocomposites. Cyclic voltammetry was used to characterise and optimise the cytosensor. Both c-MWCNTs and AuNPs increased the electron-transfer rate between the HeLa cells and the electrode, retaining good cell compatibility. The concentration of HeLa cells immobilised on the nanocomposite electrode exhibited a good correlation with the impedance values determined by electrochemical impedance spectroscopy in the range of 10
- Published
- 2022
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