1. Inhibition of translation and modification of translation factors during apoptosis induced by the DNA-damaging agent MMS in sea urchin embryos
- Author
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Julia Morales, Sandrine Boulben, Ronan Le Bouffant, Robert Bellé, Odile Mulner-Lorillon, Patrick Cormier, Mer et santé (MS), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Ribosomal Proteins ,Embryo, Nonmammalian ,MESH: Eukaryotic Initiation Factor-2 ,Protein subunit ,Eukaryotic Initiation Factor-2 ,MESH: Methyl Methanesulfonate ,Apoptosis ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,MESH: Ovum ,biology.animal ,Protein biosynthesis ,Animals ,MESH: Animals ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Translation factor ,Phosphorylation ,Sea urchin ,Ovum ,030304 developmental biology ,MESH: DNA Damage ,0303 health sciences ,MESH: Phosphorylation ,EIF4G ,MESH: Apoptosis ,organic chemicals ,fungi ,MESH: Embryo, Nonmammalian ,Translation (biology) ,MESH: Eukaryotic Initiation Factor-4G ,Cell Biology ,Methyl Methanesulfonate ,MESH: Ribosomal Proteins ,MESH: Sea Urchins ,Methyl methanesulfonate ,chemistry ,Biochemistry ,Protein Biosynthesis ,Sea Urchins ,MESH: Protein Biosynthesis ,030220 oncology & carcinogenesis ,embryonic structures ,Eukaryotic Initiation Factor-4G ,DNA Damage - Abstract
International audience; Translational control was investigated in sea urchin eggs and embryos in response to the DNA-damaging agent methyl methanesulfonate (MMS). We have shown in this report that exposure of sea urchin embryos to MMS induces drastic effects on protein synthesis activity, and on translation factors level, integrity and post-translational modifications. In response to the treatment of embryos by the DNA-damaging agent MMS, protein synthesis is inhibited independently of the translation inhibitor 4E-BP and in correlation with phosphorylation of the translation factor eIF2alpha subunit. Furthermore, a low molecular weight form of translation initiation factor eIF4G is detected correlatively with MMS-induced apoptosis. We propose that modifications of translation factors play an important role in protein synthesis modulation that occurs during DNA-damage induced apoptosis.
- Published
- 2008
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