1. The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation
- Author
-
Robert L. Ferris, Rusung Tan, Patrick S. Moore, Hyun Jin Kwun, Huilian Qin, Suzane Ramos da Silva, Yuan Chang, Univ Pittsburgh, Universidade Estadual Paulista (Unesp), and Univ British Columbia
- Subjects
Virulence Factors ,Antigen presentation ,Major histocompatibility complex class I ,Major histocompatibility complex ,medicine.disease_cause ,Article ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Virology ,MHC class I ,medicine ,Humans ,Cytotoxic T cell ,Cloning, Molecular ,Kaposi's sarcoma-associated herpesvirus ,Antigens, Viral ,Immune Evasion ,Sequence Deletion ,030304 developmental biology ,Antigen Presentation ,0303 health sciences ,biology ,Immune evasion ,Antigen processing ,Histocompatibility Antigens Class I ,Nuclear Proteins ,MHC restriction ,3. Good health ,030220 oncology & carcinogenesis ,Herpesvirus 8, Human ,biology.protein ,Latency-associated nuclear antigen 1 - Abstract
Made available in DSpace on 2013-09-27T14:54:12Z (GMT). No. of bitstreams: 1 WOS000288773100013.pdf: 1615338 bytes, checksum: 809906f90b3beafd045fea12e494091f (MD5) Previous issue date: 2011-04-10 Made available in DSpace on 2013-09-30T18:23:19Z (GMT). No. of bitstreams: 1 WOS000288773100013.pdf: 1615338 bytes, checksum: 809906f90b3beafd045fea12e494091f (MD5) Previous issue date: 2011-04-10 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:37:48Z No. of bitstreams: 1 WOS000288773100013.pdf: 1615338 bytes, checksum: 809906f90b3beafd045fea12e494091f (MD5) Made available in DSpace on 2014-05-20T13:37:48Z (GMT). No. of bitstreams: 1 WOS000288773100013.pdf: 1615338 bytes, checksum: 809906f90b3beafd045fea12e494091f (MD5) Previous issue date: 2011-04-10 NIH Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) American Cancer Society UPCI Core Facility Cancer Center KSHV LANA1, a latent protein expressed during chronic infection to maintain a viral genome, inhibits major histocompatibility complex class I (MHCI) peptide presentation in cis as a means of immune evasion. Through deletional cloning, we localized this function to the LANA1 central repeat 1 (CR1) subregion. Other CR subregions retard LANA1 translation and proteasomal processing but do not markedly inhibit LANA1 peptide processing by MHC I. Inhibition of proteasomal processing ablates LANA1 peptide presentation. Direct expression of LANA1 within the endoplasmic reticulum (ER) overcomes CR1 inhibition suggesting that CR1 acts prior to translocation of cytoplasmic peptides into the ER. By physically separating CR1 from other subdomains, we show that LANA1 evades MHC I peptide processing by a mechanism distinct from other herpesviruses including Epstein-Barr virus (EBV). Although LANA1 and EBV EBNA1 are functionally similar, they appear to use different mechanisms to evade host cytotoxic T lymphocyte surveillance. (C) 2011 Elsevier B.V. All rights reserved. Univ Pittsburgh, Inst Canc, Canc Virol Program, Pittsburgh, PA 15213 USA São Paulo State Univ, Botucatu Sch Med, Dept Pathol, São Paulo, Brazil Univ British Columbia, British Columbia Childrens Hosp, Dept Pathol & Lab Med, Vancouver, BC V5Z 1M9, Canada Univ Pittsburgh, Med Ctr, Dept Otolaryngol, Pittsburgh, PA 15213 USA Univ Pittsburgh, Med Ctr, Dept Immunol, Pittsburgh, PA 15213 USA Univ Pittsburgh, Inst Canc, Canc Immunol Program, Pittsburgh, PA 15213 USA São Paulo State Univ, Botucatu Sch Med, Dept Pathol, São Paulo, Brazil NIH: NCI121930 CAPES: BEX1049/05-4 UPCI: P30 CA047904
- Published
- 2011