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Vibration analysis is important for the evaluation of Natural frequencies and their effect on the bone for supportive equipment used while walking, running conditions. A femur bone is longest and taking the maximum load while standing, walking and running conditions. In present experimental research two types of conditions are used to evaluate natural frequencies of dead bone. While carried out experiment, FFT analyzer is used which gives desirable results of Natural frequencies. For analytical analysis, consistent mass matrices are used and natural frequencies are compared between analytical method and FFT analyzer. Average difference error between the two readings by consistent mass matrices and FFT analyzer is found to be less than one in first mode.