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This study investigated the effect of ELOVL6 (elongation of very long chain fatty acids protein 6) and its underlying mechanism on lipid metabolism in bovine adipocytes. The ELOVL6 gene was overexpressed in bovine adipocytes by adenoviruses, and RNA sequencing was performed. Overexpression of ELOVL6 showed reduced proportions of C14:0 (Myristic) and C16:0 (palmitate) fatty acids and increased proportions of C18.0 (stearate) and C20:4n6 (arachidonic) fatty acids in adipocytes. In addition, a total of 2170 differentially expressed genes (DEGs) were found, containing 1802 up-regulated and 368 down-regulated genes. KEGG pathway analysis revealed that the down-regulated genes were linked with the regulation of lipolysis and the Wnt signaling pathway. The up-regulated genes were mainly involved in the FoxO signaling pathway; the PI3K-Akt signaling pathway; and the cAMP signaling pathway. In conclusion, our results suggest that ELOVL6 could affect the fatty acid composition in bovine adipocytes. We identified numerous related DEGs and pathways, which may provide a basis for studying the function and molecular mechanism of the ELOVL6 gene in regulating lipid metabolism.

After weaning, piglets suffer from various stresses, and their immune function declines, causing diarrhoea and death. In recent years, the intestinal health of piglets has been a hot topic in the field of swine nutrition and feedstuff research. The present work aimed to investigate the effects of Clostridium butyricum (C. butyricum) on plasma immune function, antioxidant activity and plasma metabolomics of weaned piglets. A total of 90 weaning piglets of average weight (6.22 ± 0.24 kg BW) and 28±2 days old were randomly divided into three groups: control diet, control diet + 0.1 g/kg antibiotic (colistin sulphate), and control diet + 5 × 108 CFU/kg C. butyricum. The pigs were divided into 6 pens per group with 5 piglets per pen for 21 days. Our results showed that the diet supplemented with 5 × 108 CFU/kg C. butyricum significantly reduced the levels of plasma glucose, total protein, high-density lipoprotein, phosphorus, IL-6, TNF-α, MDA and increased the levels of albumin, triglyceride, low-density lipoprotein, IgG, C3, SOD activity, T-AOC in the plasma (P