1. Recombinant Sendai virus induces T cell immunity against respiratory syncytial virus that is protective in the absence of antibodies
- Author
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Elias Hobeika, Wolfgang J. Neubert, Gert Zimmer, Sascha Bossow, Simone Vallbracht, Kirsten Richter, Stephan Ehl, Georg Herrler, and Brigitte Voges
- Subjects
Cellular immunity ,T-Lymphocytes ,viruses ,T cell ,Immunology ,Respiratory Syncytial Virus Infections ,Biology ,Sendai virus ,Antibodies ,Virus ,Mice ,Immune system ,medicine ,Animals ,Neutralizing antibody ,Mice, Inbred BALB C ,biology.organism_classification ,Virology ,Respiratory Syncytial Viruses ,Vaccination ,medicine.anatomical_structure ,Solubility ,biology.protein ,Antibody ,Genetic Engineering ,Viral Fusion Proteins - Abstract
Respiratory syncytial virus (RSV) causes severe respiratory disease in infants and a vaccine is highly desirable. The fusion (F) protein of RSV is an important vaccine target, but the contribution of F-specific T cells to successful vaccination remains unclear. We studied the immune response to vaccination of mice with a recombinant Sendai virus expressing RSV F (rSeV F). rSeV F induced protective neutralizing antibody and RSV F-specific CTL responses. T cell immunity was stronger than that induced by recombinant vaccinia virus (rVV F), a well characterized reference vector. Vaccination of antibody-deficient mice showed that vaccine-induced RSV F-specific T cells were sufficient for protective immunity. rSeV F induced T cell immunity in the presence of neutralizing antibodies, which did not impair the vaccine response. Although the F protein only contains a subdominant CTL epitope, vaccination with rSeV F is sufficient to induce protective T cell immunity against RSV in mice.
- Published
- 2007
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