1. Flow Dynamics and HSPC Homing in Bone Marrow Microvessels
- Author
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Kishor K. Sivaraj, Ralf H. Adams, M. Gabriele Bixel, Stefan Butz, Dietmar Vestweber, Anjali P. Kusumbe, and Saravana K. Ramasamy
- Subjects
Resource ,0301 basic medicine ,bone marrow ,Hemodynamics ,Bone Marrow Cells ,Mice, Transgenic ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,stem cell homing ,Cell Movement ,Stress, Physiological ,medicine ,Animals ,deep tissue imaging ,Progenitor cell ,lcsh:QH301-705.5 ,blood flow velocities ,hematopoietic parameters ,Hematopoietic stem cell ,Blood flow ,Anatomy ,Hematopoietic Stem Cells ,wall shear stress ,3. Good health ,Mice, Inbred C57BL ,Haematopoiesis ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,030220 oncology & carcinogenesis ,Microvessels ,hematopoietic stem cell ,intravital imaging ,Bone marrow ,Shear Strength ,Blood Flow Velocity ,microvasculature ,Homing (hematopoietic) ,Biomedical engineering ,Cranial window - Abstract
Summary Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing behavior. With our imaging setup, we determined flow patterns at cellular resolution, blood flow velocities and wall shear stress in small arterial vessels and highly branched sinusoidal capillaries, and the cellular dynamics of hematopoietic stem and progenitor cell homing., Graphical Abstract, Highlights • Detailed 3D reconstruction of the calvarial bone marrow microvasculature • Blood flow dynamics at cellular resolution in bone microvessels • Detailed flow map with hemodynamic parameters in arteries and highly branched sinusoids • Cellular dynamics of HSPC homing to bone marrow sinusoids, Bixel et al. use intravital two-photon imaging to determine blood flow patterns at cellular resolution and hemodynamic parameters in individual arterial vessels and sinusoidal capillaries in the bone marrow microvasculature. They report detailed information on the dynamics of hematopoietic stem and progenitor cell homing to highly branched bone marrow sinusoids.
- Published
- 2017