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Transformation products (TPs) of organic micropollutants are still rarely considered in monitoring of wastewater and aquatic environments. For example, occurrence data of ozonated TPs in full-scale wastewater systems is largely lacking. In this study, the efficiency of a suspect screening strategy including 245 previously reported compounds and their TPs was evaluated for assessing the occurrence of different compound classes and their ozonated TPs in wastewater samples collected at different steps of an advanced treatment process including ozonation. After applying blank subtraction and filtering by mass accuracy (5 ppm tolerance), peak height (minimum 1000 counts) and isotopic pattern score (≥ 80%) 189 of the 245 compounds were detected. A decrease in relative concentration levels was observed for parent compounds in wastewater after ozonation and after a subsequent biological treatment process, while formation of tentative TPs after ozonation accompanied by subsequent degradation in a following biological treatment step was found. Plausibility of structural assignments for tentatively identified TPs could be successfully tested by using relative retention time information as comparison criteria. Overall, the screening approach was fast and successful and can be expanded to other compound classes and TPs where reference standards are not readily available.

Artificial sweeteners have been recognized as emerging contaminants due to their wide application, environmental persistence and ubiquitous occurrence. Among them, acesulfame has attracted much attention. After being discharged into the environment, acesulfame undergoes photolysis naturally. However, acesulfame photodegradation behavior and identity of its transformation products, critical to understanding acesulfame's environmental impact, have not been thoroughly investigated. The present study aimed to fill this knowledge gap by a laboratory simulation study in examining acesulfame transformation products and pathways under UV-C photolysis in the presence of TiO2. Photodegradation products of acesulfame were isolated and analyzed using the LC-IM-QTOF-MS coupled with LC Ion Trap MS in the MSn mode. Our results show six new transformation products that have not been previously identified. The molecular structures and transformation pathways were proposed. Further embryotoxicity tests showed that acesulfame transformation products at the low g L−1 level produced significant adverse effects in tail detachment, heart rate, hatching rate and survival rate during fish embryo development. The identification of additional transformation products with proposed transformation pathways of acesulfame, the increased toxicity of acesulfame after photolysis, and the fact that the accumulation of acesulfame transformation products is increasingly likely make acesulfame contamination even more important. Water resource control agencies need to consider legislation regarding acesulfame and other artificial sweeteners, while further studies are carried out, in order to protect the safety of this most vital resource.