1. Quantitative analysis of natural cyclodextrins by high-performance liquid chromatography with pulsed amperometric detection: Application to cell permeation study
- Author
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Heli Raatikainen, Pekka Keski-Rahkonen, Tarja Toropainen, Marko Lehtonen, Tomi Järvinen, and Pekka Jarho
- Subjects
chemistry.chemical_classification ,Cyclodextrins ,Cell Membrane Permeability ,Chromatography ,Cyclodextrin ,Resolution (mass spectrometry) ,Clinical Biochemistry ,Extraction (chemistry) ,Reproducibility of Results ,Cell Biology ,General Medicine ,Repeatability ,Reference Standards ,Permeation ,Sensitivity and Specificity ,Biochemistry ,High-performance liquid chromatography ,Amperometry ,Analytical Chemistry ,chemistry ,Calibration ,Electrochemistry ,Quantitative analysis (chemistry) ,Chromatography, High Pressure Liquid - Abstract
Simple HPLC-PAD methods were developed for quantitation of cyclodextrins (CDs) in aqueous matrices from in vitro cell permeation studies. C-18 solid-phase extraction was used for sample pretreatment. Samples were analysed using acetonitrile-water mobile phase with post-column alkalization by 0.5M NaOH. Zorbax SB-Aq (for alpha-CD) and Zorbax SB-Phenyl (for beta-CD and gamma-CD) columns gave excellent peak shape and sufficient resolution of CD to glucose (2.7-3.2). The methods showed good concentration-response relationship (ror = 0.999), precision (RSD% 0.7-5.1), repeatability (RSD% 3.4-13.7) and accuracy (87-107%). The limits of quantitation were 0.78, 0.46 and 0.52 microg/ml for alpha-CD, beta-CD and gamma-CD (RSD% of 10.6, 8.1 and 16.3, respectively).
- Published
- 2008
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