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2. Polyploidy control in hepatic health and disease

Author

Thomas Reiberger, Felix Eichin, Andreas Villunger, and Valentina C. Sladky

Subjects
0301 basic medicine, Hepatology, Liver Neoplasms, Disease, Biology, Prognosis, medicine.disease, Malignant transformation, Polyploidy, 03 medical and health sciences, Liver disease, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Liver, Centrosome, Hepatocellular carcinoma, Hepatocyte, medicine, Cancer research, Humans, 030211 gastroenterology & hepatology, Ploidy, Liver cancer, Signal Transduction
Abstract

Summary A balanced increase in DNA content (ploidy) is observed in some human cell types, including bone-resorbing osteoclasts, platelet-producing megakaryocytes, cardiomyocytes or hepatocytes. The impact of increased hepatocyte ploidy on normal physiology and diverse liver pathologies is still poorly understood. Recent findings suggest swift genetic adaptation to hepatotoxic stress and the protection from malignant transformation as beneficial effects. Herein, we discuss the molecular mechanisms regulating hepatocyte polyploidisation and its implication for different liver diseases and hepatocellular carcinoma. We report on centrosomes’ role in limiting polyploidy by activating the p53 signalling network (via the PIDDosome multiprotein complex) and we discuss the role of this pathway in liver disease. Increased hepatocyte ploidy is a hallmark of hepatic inflammation and may play a protective role against liver cancer. Our evolving understanding of hepatocyte ploidy is discussed from the perspective of its potential clinical application for risk stratification, prognosis, and novel therapeutic strategies in liver disease and hepatocellular carcinoma.

Published
2021
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3. PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis

Author

Kim, Ju Youn, primary, Wang, Lily Q., additional, Sladky, Valentina C., additional, Oh, Tae Gyu, additional, Liu, Junlai, additional, Trinh, Kaitlyn, additional, Eichin, Felix, additional, Downes, Michael, additional, Hosseini, Mojgan, additional, Jacotot, Etienne D., additional, Evans, Ronald M., additional, Villunger, Andreas, additional, and Karin, Michael, additional

Published
2022
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5. Education for innovation and entrepreneurship in the food system: the Erasmus+ BoostEdu approach and results

Author

Viaggi, Davide, primary, Barrera, Cristina, additional, Castelló, María Luisa, additional, Dalla Rosa, Marco, additional, Heredia, Ana, additional, Hobley, Timothy J., additional, Knöbl, Christoph F., additional, Materia, Valentina C., additional, Xu, Sunny Mosangzi, additional, Romanova, Gergana, additional, Russo, Silvia, additional, Seguí, Lucía, additional, and Viereck, Nanna, additional

Published
2021
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8. PIDDosome-SCAP Crosstalk Controls Diet Dependent Transition from Simple Steatosis to Steatohepatitis

Author

Kim, Ju Youn, primary, Wang, Lily, additional, Sladky, Valentina C., additional, Oh, Tae Gyu, additional, Liu, Junlai, additional, Trinh, Kaitlyn, additional, Eichin, Felix, additional, Downes, Michael, additional, Hosseini, Mojgan, additional, Jacotot, Etienne D., additional, Evans, Ronald M., additional, Villunger, Andreas, additional, and Karin, Michael, additional

Published
2021
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11. E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration

Author

Sladky, Valentina C., primary, Knapp, Katja, additional, Soratroi, Claudia, additional, Heppke, Julia, additional, Eichin, Felix, additional, Rocamora-Reverte, Lourdes, additional, Szabo, Tamas G., additional, Bongiovanni, Laura, additional, Westendorp, Bart, additional, Moreno, Eva, additional, van Liere, Elsbeth A., additional, Bakker, Bjorn, additional, Spierings, Diana C.J., additional, Wardenaar, René, additional, Pereyra, David, additional, Starlinger, Patrick, additional, Schultze, Simon, additional, Trauner, Michael, additional, Stojakovic, Tatjana, additional, Scharnagl, Hubert, additional, Fava, Luca L., additional, Foijer, Floris, additional, de Bruin, Alain, additional, and Villunger, Andreas, additional

Published
2020
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14. Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications

Author

Paul Price, Sharon Cates, Valentina C. Ciccarone, Mary Lynn Tilkins, Elise C. Ohki, Brian Dalby, and Adam Harris

Subjects
animal structures, viruses, Cell Count, Biology, Transfection, Hippocampus, General Biochemistry, Genetics and Molecular Biology, Genes, Reporter, RNA interference, Animals, Humans, Cationic liposome, Viability assay, RNA, Small Interfering, Luciferases, Molecular Biology, Cells, Cultured, Cerebral Cortex, Neurons, Liposome, fungi, DNA, Lamin Type A, beta-Galactosidase, Lipids, In vitro, Culture Media, Rats, Cell biology, Lipofectamine, Reagent, Liposomes, embryonic structures, RNA Interference, Plasmids
Abstract

Lipofectamine 2000 is a cationic liposome based reagent that provides high transfection efficiency and high levels of transgene expression in a range of mammalian cell types in vitro using a simple protocol. Optimum transfection efficiency and subsequent cell viability depend on a number of experimental variables such as cell density, liposome and DNA concentrations, liposome-DNA complexing time, and the presence or absence of media components such as antibiotics and serum. The importance of these factors in Lipofectamine 2000 mediated transfection will be discussed together with some specific applications: transfection of primary neurons, high throughput transfection, and delivery of small interfering RNAs.

Published
2004
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15. Improving the transfection efficiency of post-mitotic neurons

Author

E.C Ohki, Paul Price, Valentina C. Ciccarone, and Mary Lynn Tilkins

Subjects
Central Nervous System, animal structures, viruses, Genetic Vectors, Cell, Biology, Hippocampal formation, Transfection, Hippocampus, Virus, Fetus, Genes, Reporter, Glial Fibrillary Acidic Protein, medicine, Animals, Cation Exchange Resins, Mitosis, Cells, Cultured, Cerebral Cortex, Neurons, Reporter gene, General Neuroscience, Electroporation, fungi, beta-Galactosidase, Immunohistochemistry, Lipids, Molecular biology, Rats, Cell biology, medicine.anatomical_structure, Lipofectamine, Phosphopyruvate Hydratase, embryonic structures, Indicators and Reagents
Abstract

The transfection of post-mitotic cells, including primary cortical and hippocampal neurons, has proven for the most part to be inefficient. Methods such as DNA/Ca++ phosphate co-precipitation, electroporation, cationic lipids and micro-injection are often toxic to the cell and rarely give a transfection efficiency exceeding 3% of the surviving culture. Virus transfection methods using modified viruses such as adeno and semliki-forest virus have been shown to be more efficient but the procedures are often time consuming and virus infections may interfere with protein processing. In this study, we evaluated the transfection efficiency of cells from E18 rat embryonic cortical and hippocampal tissues using three cationic lipids: LIPOFECTAMINE, LIPOFECTAMINE Plus, and LIPOFECTAMINE 2000. The method of transfection was by a traditional reporter gene beta-galactosidase (pCMV.SPORT-beta-gal). Results show that out of the three cationic lipids tested, LIPOFECTAMINE 2000 allows for a significantly higher transfection efficiency. Transfection efficiency with LIPOFECTAMINE or LIPOFECT-AMINE Plus was

Published
2001
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18. A specific combination of phytoestrogens ameliorates the mitochondrial dysfunction in Leber's hereditary optic neuropathy

Author

Vincenzo Petrozza, Annalinda Pisano, Anna Ghelli, Valerio Carelli, Massimiliano Mancini, Carmela Preziuso, Carla Giordano, Valentina C. Tropeano, and Luciano Petruzziello

Subjects
medicine.medical_specialty, business.industry, Leber's hereditary optic neuropathy, Cell Biology, medicine.disease, chemistry.chemical_compound, Endocrinology, chemistry, Internal medicine, Molecular Medicine, Medicine, Phytoestrogens, business, Molecular Biology
Published
2013
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