1. Epidemic multidrug-resistant Acinetobacter baumannii related to European clonal types I and II in Rome (Italy)
- Author
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D'Arezzo, S., Capone, A., Petrosillo, N., Visca, P., Grab, Ballardini, M., Bartolini, S., Bordi, E., Di Stefano, A., Galie, M., Minniti, R., Meledandri, M., Pacciani, L., Parisi, G., Prignano, G., Santini, Claudio, Valmarin, M., Venditti, Mario, Ziantoni, S., S., Darezzo, A., Capone, N., Petrosillo, Visca, Paolo, and ON BEHALF OF, Grab
- Subjects
Acinetobacter baumannii ,DNA, Bacterial ,Microbiology (medical) ,Genotype ,Rome ,Biology ,Epidemiological typing ,Integron ,Genetic analysis ,beta-Lactamases ,Integrons ,Microbiology ,Antibiotic resistance ,Bacterial Proteins ,multidrug resistance ,acinetobacter baumannii ,epidemiology ,integrons ,nosocomial infection ,typing ,Drug Resistance, Multiple, Bacterial ,Intensive care ,Cluster Analysis ,Humans ,Hospital infection ,Typing ,Gene Rearrangement ,Cross Infection ,Molecular Epidemiology ,Molecular epidemiology ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,DNA Fingerprinting ,Virology ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Random Amplified Polymorphic DNA Technique ,Multiple drug resistance ,Intensive Care Units ,Infectious Diseases ,biology.protein ,bacteria ,Acinetobacter Infections - Abstract
The molecular epidemiology and the genetic basis of antibiotic resistance in 88 multidrug-resistant (MDR) Acinetobacter baumannii strains isolated during 18 months from infected patients in seven intensive care units (ICUs) in Rome were investigated. Random amplified polymorphic DNA and macrorestriction analysis identified two predominant clonal types, genetically related to the European epidemic clones I (type 2) and II (type 1), accounting for 98.9% of A. baumannii ICU isolates. Type 1 was isolated from all ICUs under survey. Class 1 integrons of 2.2 and 2.5 kb were detected in type 1 and type 2 isolates, respectively. The integron structures were similar to those previously determined for epidemic A. baumannii strains from various European countries, and suggestive of integron rearrangement/exchange among isolates related to the European epidemic clones I and II. Carbapenem resistance was associated with the presence of the blaOXA-58 gene in type 1 isolates. The results indicate that the A. baumannii type 1 clone has a high potential of spreading among hospitals.
- Published
- 2009
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