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Start Over Author "Venter, Estelle" Publisher elsevier bv
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2. Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen

Author

Annandale, C Henry, Smuts, Mario P, Ebersohn, Karen, du Plessis, Lizette, Venter, Estelle H, Stout, Tom A E, LS Voortplanting Inwendige Ziekten, dES/dFAH FR, and LS Klinische Reproductie

Subjects
Male, 0301 basic medicine, 040301 veterinary sciences, medicine.medical_treatment, Semen, Bull, Capripoxvirus, Specimen Handling, 0403 veterinary science, Andrology, 03 medical and health sciences, Semen quality, Cryoprotective Agents, Endocrinology, Food Animals, Lumpy skin disease, medicine, Animals, Sanitation methods, Sperm plasma membrane, Cryopreservation, biology, Artificial insemination, 04 agricultural and veterinary sciences, General Medicine, biology.organism_classification, medicine.disease, Spermatozoa, Lumpy skin disease virus, 030104 developmental biology, Cattle, Animal Science and Zoology, Viral load, Semen Preservation
Abstract

Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR. The ejaculate was split into a control sample (C), a sample spiked with a 4 log TCID50 dose of an LSDV isolate (HD) and a 103 dilution of the virus suspension (ND) and frozen routinely. Two straws from each of the different semen treatment groups (HD, ND and C) were subsequently thawed and subjected to swim-up, single layer centrifugation, Percoll® density gradient and a Percoll® density gradient with added trypsin. For one set of straws, semen quality variables were recorded, and viral DNA status determined using PCR; the other set was used for positive staining electron microscopy. Samples determined to be positive for LSDV DNA by PCR were then subjected to virus isolation (VI). Complete elimination of LSDV from semen did not occur with use of any of the processing methods. Trypsin did reduce the viral load, and eliminated LSDV from the ND sample, but severely negatively influenced semen quality. The LSDV virions, as assessed by electron microscopy, were associated with the sperm plasma membrane. Further investigation is needed to establish the efficacy of immuno-extenders for rendering semen free from LSDV.

Published
2018
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