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Start Over Author "Wang, Bingyun" Publisher elsevier bv
14 results on '"Wang, Bingyun"'

3. Protegrin-1 inhibits porcine ovarian granulosa cell apoptosis from H2O2-induced oxidative stress via the PERK/eIF2α/CHOP signaling pathway in vitro

Author

Yufeng Lin, Wang Bingyun, Xiaoshu Zhan, Julang Li, Chen Zhisheng, Chen Shengfeng, Jiawei Yao, Can-Ying Liu, Bai Yinshan, Hui Zhang, Xuan Li, and Bo Pan

Subjects
endocrine system, Ovarian Granulosa Cell, Equine, Kinase, Chemistry, Endoplasmic reticulum, Follicular atresia, CHOP, medicine.disease_cause, Cell biology, Food Animals, Apoptosis, medicine, Animal Science and Zoology, Signal transduction, Small Animals, Oxidative stress
Abstract

In mammals, oxidative stress-induced apoptosis of granulosa cells is one of the major causes of follicular atresia, affecting ovarian physiological function. Protegrin-1 (PG-1) is an antimicrobial peptide with effective antimicrobial activity, immunomodulatory function, and porcine growth-promoting effects. PG-1 has been detected in porcine ovaries follicles. This study aimed to investigate the effect of PG-1 on oxidative stress-induced apoptosis of porcine ovarian granulosa cells and the underlying molecular mechanism. Granulosa cells were obtained from porcine follicles and treated with H2O2 to establish the oxidative stress model, and then treated with or without PG-1 (10 μg/mL). PG-1 significantly suppressed H2O2-induced apoptosis in granulosa cells after 24 h of treatment. Furthermore, these results revealed that PG-1 increased the mRNA and protein expression of anti-apoptotic B cell lymphoma/leukemia 2 (BCL2) and the BCL2/Bcl-2-associated X protein (BAX) ratio while decreasing the expression of pro-apoptotic BAX and active caspase-3. Using western blot analysis, it was found that PG-1 decreased the phosphorylation of RNA-like endoplasmic reticulum kinase (PERK) and the α-subunit of eukaryotic initiation factor 2 (eIF2α) as well as the protein expression level of CCAAT enhancer-binding protein homologous protein (CHOP), all of which were increased by H2O2. Moreover, inhibitors against PERK and phospho-eIF2ɑ both suppressed the H2O2-induced granulosa cells apoptosis and enhanced the anti-apoptosis effect of PG-1. Taken together, our findings demonstrated that PG-1 inhibited porcine ovarian granulosa cell apoptosis from oxidative stress via the PERK/eIF2α/CHOP signaling pathway in vitro, which suggests the novel regulatory function of the antimicrobial peptide in the ovary.

Published
2022

6. Protegrin-1 inhibits porcine ovarian granulosa cell apoptosis from H2O2-induced oxidative stress via the PERK/eIF2α/CHOP signaling pathway in vitro

Author

Li, Xuan, primary, Lin, Yufeng, additional, Yao, Jiawei, additional, Pan, Bo, additional, Zhan, Xiaoshu, additional, Chen, Zhisheng, additional, Bai, Yinshan, additional, Zhang, Hui, additional, Wang, Bingyun, additional, Chen, Shengfeng, additional, Li, Julang, additional, and Liu, Canying, additional

Published
2022
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