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Your search keyword '"Westerhout, Ellen M."' showing total 6 results
Start Over Author "Westerhout, Ellen M." Publisher elsevier bv
6 results on '"Westerhout, Ellen M."'

1. Dendritic cells potently purge latent HIV-1 beyond TCR-stimulation, activating the PI3K-Akt-mTOR pathway

Author

van Montfort, Thijs, primary, van der Sluis, Renée, additional, Darcis, Gilles, additional, Beaty, Doyle, additional, Groen, Kevin, additional, Pasternak, Alexander O., additional, Pollakis, Georgios, additional, Vink, Monique, additional, Westerhout, Ellen M., additional, Hamdi, Mohamed, additional, Bakker, Margreet, additional, van der Putten, Boas, additional, Jurriaans, Suzanne, additional, Prins, Jan H., additional, Jeeninga, Rienk, additional, Thomas, Adri A.M., additional, Speijer, Dave, additional, and Berkhout, Ben, additional

Published
2019
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3. Liquid chromatography-tandem mass spectrometric assay for the PI3K/mTOR inhibitor GSK2126458 in mouse plasma and tumor homogenate

Author

Dolman, M. Emmy M, Westerhout, Ellen M., Hamdi, Mohamed, Schellens, Jan H M, Beijnen, Jos H., Sparidans, Rolf W., Sub Clinical Pharmacology, Sub Medicinal Chemistry & Chemical biol., Molecular Pharmacy, Pharmacoepidemiology and Clinical Pharmacology, Sub Clinical Pharmacology, Sub Medicinal Chemistry & Chemical biol., Molecular Pharmacy, Pharmacoepidemiology and Clinical Pharmacology, Oncology, Cancer Center Amsterdam, Amsterdam Public Health, and Human Genetics

Subjects
Spectrometry, Mass, Electrospray Ionization, Electrospray, Bioanalysis, Mouse, Tumor homogenate, Clinical Biochemistry, Pharmaceutical Science, GSK2126458, Analytical Chemistry, Mice, Neuroblastoma, Plasma, chemistry.chemical_compound, Tandem Mass Spectrometry, Neoplasms, Drug Discovery, Animals, Protein precipitation, LC-MS/MS, Acetonitrile, Protein Kinase Inhibitors, Spectroscopy, Phosphoinositide-3 Kinase Inhibitors, Sulfonamides, Chromatography, Elution, TOR Serine-Threonine Kinases, Selected reaction monitoring, Reproducibility of Results, Triple quadrupole mass spectrometer, Dilution, Pyridazines, chemistry, Calibration, Quinolines, Female, Chromatography, Liquid
Abstract

A quantitative bioanalytical liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay for GSK2126458, a dual PI3K/mTOR inhibitor, was developed and validated. Plasma and tumor homogenate samples were pre-treated using protein precipitation with acetonitrile containing dabrafenib as internal standard. After dilution with water, the extract was directly injected into the reversed-phase liquid chromatographic system. The eluate was transferred into the electrospray interface with positive ionization and compounds were detected in the selected reaction monitoring mode of a triple quadrupole mass spectrometer. The assay was completely validated for plasma in a 4–4000 ng/ml calibration range with r 2 = 0.9996 ± 0.0003 using double logarithmic calibration ( n = 5). Within-run precisions ( n = 6) were 2.0–5.3% and between-run (3 runs; n = 18) precisions 2.7–5.8%. Accuracies were between 101 and 105% for the whole calibration range. The drug was sufficiently stable under all relevant analytical conditions. Finally, the assay was successfully applied to determine plasma and tumor drug levels after oral administration of GSK2126458 to mice with AMC711T neuroblastoma xenografts.

Published
2015

4. Dendritic Cells Potently Purge Latent HIV-1 in TCR-Activated Cells via the PI3K-Akt-mTOR Pathway: Implications for Shock and Killl Strategies and Reservoir Analysis

Author

van Montfort, Thijs, primary, van der Sluis, Renne, additional, Darcis, Gilles, additional, Beaty, Doyle, additional, Groen, Kevin, additional, Pasternak, Alexander O., additional, Pollakis, Georgios, additional, Vink, Moniek, additional, Westerhout, Ellen M., additional, Hamdi, Mohamed, additional, Bakker, Margreet, additional, van der Putten, Boas, additional, Jurriaans, Suzanne, additional, Prins, Jan, additional, Jeeninga, Rienk, additional, Thomas, Adri A.M., additional, Speijer, Dave, additional, and Berkhout, Ben, additional

Published
2018
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