19 results on '"Wonyong Kim"'
Search Results
2. Fr533 POTENTIAL BIOMARKERS PREDICTING LONG-TERM REMISSION WITH ADALIMUMAB THERAPY IN PATIENTS WITH ULCERATIVE COLITIS BY FECAL METABOLOME, LIPIDOME, AND MICROBIOME PROFILING
- Author
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Eun Soo Kim, Hyung-Kyoon Choi, Sung Jae Shin, Soo Jung Park, Kang-Moon Lee, You-Sun Kim, Chang Hwan Choi, Dennis Teng, Jong Pil Im, Hyo Jong Kim, Sung-Ae Jung, Hyun-Soo Kim, Hyungkil Kim, Wonyong Kim, Tae Oh Kim, Seung Yong Shin, Young Ho Kim, Jun Lee, Jong-Hwa Kim, Sang-Bum Kang, Dong Il Park, Jiwon Kim, Eun Sun Kim, and Seok-Young Kim
- Subjects
medicine.medical_specialty ,Hepatology ,business.industry ,Adalimumab therapy ,Gastroenterology ,Lipidome ,medicine.disease ,Ulcerative colitis ,Potential biomarkers ,Internal medicine ,medicine ,Metabolome ,In patient ,Microbiome ,business ,Feces - Published
- 2021
3. Differentiation of RotaTeq ® vaccine strains from wild-type strains using NSP3 gene in reverse transcription polymerase chain reaction assay
- Author
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Van Thai Than, Wonyong Kim, Sunyoung Jeong, and Inseok Lim
- Subjects
0301 basic medicine ,viruses ,Virulence ,Biology ,medicine.disease_cause ,Virology ,Virus ,law.invention ,Microbiology ,Vaccination ,Reverse transcription polymerase chain reaction ,03 medical and health sciences ,fluids and secretions ,030104 developmental biology ,law ,Rotavirus ,Genotype ,medicine ,Multiplex ,Polymerase chain reaction - Abstract
RotaTeq® is a live attenuated human-bovine reassortant vaccine against rotaviruses that is used worldwide. However, shedding of the virus used in RotaTeq® has been detected in the feces of children following vaccination by the oral route, possibly affecting community immunity. Therefore, a simple and efficient method to discriminate between virulent and RotaTeq® vaccine strains is required. In this study, a novel one-step multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay targeting the NSP3 gene was developed to detect RotaTeq® vaccine strains in fecal samples. RotaTeq® vaccine viruses were successfully distinguished from known wild-type rotavirus genotypes. In addition, the developed assay was able to detect rotaviruses in clinical stool samples obtained from South Korea during the 2011-2013 rotavirus seasons. Of the 1106 stool specimens from children with acute gastroenteritis that were screened, 286 rotaviruses were genotyped. RotaTeq® vaccine strains were identified in 39 samples (13.6%). The novel RT-PCR assay that was developed could be used to detect and discriminate between RotaTeq® vaccine strains that are shed in fecal matter, and to estimate the quantification of virus that has been shed after vaccination.
- Published
- 2016
4. Evidence of multiple reassortment events of feline-to-human rotaviruses based on a rare human G3P[9] rotavirus isolated from a patient with acute gastroenteritis
- Author
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Tinh Huu Nguyen, Van Thai Than, Hien Dang Thanh, and Wonyong Kim
- Subjects
Rotavirus ,0301 basic medicine ,Lineage (genetic) ,Genotype ,viruses ,Immunology ,Reassortment ,Genome, Viral ,Biology ,medicine.disease_cause ,Microbiology ,Genome ,Rotavirus Infections ,Feces ,03 medical and health sciences ,medicine ,Animals ,Humans ,Immunology and Allergy ,Gene ,Phylogeny ,Genetics ,NSP1 ,General Veterinary ,Phylogenetic tree ,virus diseases ,Sequence Analysis, DNA ,General Medicine ,Virology ,Gastroenteritis ,030104 developmental biology ,Infectious Diseases ,Child, Preschool ,Acute Disease ,Cats ,RNA, Viral ,Female ,Reassortant Viruses - Abstract
A rare human/feline-like rotavirus G3P[9] strain, CAU14-1-262, from a 2-year-old girl with severe gastroenteritis was isolated and sequenced. The 11 gene segments of the CAU14-1-262 strain possessed a novel genotype constellation, G3-P[9]-I3-R3-C3-M3-A3-N3-T1-E3-H6, which was identified for the first time. Phylogenetic analysis of this strain identified the following genome origins: VP7, VP4, VP6, VP1-VP3, NSP1, NSP2, and NSP4 genes possessed an AU-1-like genotype 3 constellation with high sequence identity to those of the feline and human/feline-like rotaviruses; NSP5 possessed a H6 lineage, with highest sequence identity to the human/feline-like E2541 strain; and the NSP3 gene possessed a Wa-like genotype 1 constellation with high sequence identity to those of the of human rotaviruses. These results provided evidence of multiple reassortment events in G3P[9] rotavirus CAU14-1-262 and possibility of feline-to-human interspecies transmission.
- Published
- 2016
5. Tu1890 THE EFFICACY AND SAFETY OF ADALIMUMAB FOR PATIENTS WITH MODERATELY TO SEVERELY ACTIVE ULCERATIVE COLITIS AND PREDICTORS OF RESPONSE IN KOREA
- Author
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Jong-Hwa Kim, Kang-Moon Lee, Do Hyun Kim, Young Keun Kim, Chang Hwan Choi, Sung Jae Shin, Eun-Soo Kim, Hyungkil Kim, You-Sun Kim, Jun Lee, Dennis Teng, Soo Jung Park, Seung Yong Shin, Hyo-Jong Kim, Tae-Oh Kim, Sang-Bum Kang, Wonyong Kim, Jong Pil Im, Eun Sun Kim, Sung-Ae Jung, Hyun Soo Kim, Dong Il Park, and Jiwon Kim
- Subjects
medicine.medical_specialty ,Hepatology ,business.industry ,Internal medicine ,Gastroenterology ,Adalimumab ,medicine ,business ,medicine.disease ,Ulcerative colitis ,medicine.drug - Published
- 2020
6. A systematic review of genetic diversity of human rotavirus circulating in South Korea
- Author
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Van Thai Than, Sunyoung Jeong, and Wonyong Kim
- Subjects
Rotavirus ,Microbiology (medical) ,medicine.medical_specialty ,Genotype ,viruses ,Molecular Sequence Data ,Rotavirus Infections ,Genome, Viral ,Biology ,Serogroup ,medicine.disease_cause ,Microbiology ,fluids and secretions ,Republic of Korea ,Human rotavirus ,Epidemiology ,Genetics ,medicine ,Humans ,Amino Acid Sequence ,Antigens, Viral ,Molecular Biology ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Recombination, Genetic ,Genetic diversity ,Phylogenetic tree ,Rotavirus Vaccines ,Genetic Variation ,virus diseases ,Sequence Analysis, DNA ,Virology ,Rotavirus vaccine ,Infectious Diseases ,Population Surveillance ,RNA, Viral ,Capsid Proteins ,Sequence Alignment - Abstract
Rotavirus infections continue to be the leading cause of severe diarrhea in young Korean children. Rotavirus data acquired from uninterrupted surveillance studies between 1989 and 2009 in South Korea were analyzed to better understand the genetic diversity and evolution. The relationship between rotaviruses and the currently licensed rotavirus vaccine viruses was also examined. The most prevalent rotavirus strains, with genotype G1P[8], followed by G3P[8], G4P[6], and G2P[4], accounted for approximately 76.7% of the total identified strains, and more recently, rotavirus G9P[8] has significance increased to be the fifth most common genotype. Phylogenetic analyses underscored the heterogeneity between viral populations within each genotype, with different lineages and sub-lineages. Although the currently licensed rotavirus vaccines are effective, safe, and economical, additional data from rotavirus monitoring is necessary to evaluate the efficacy of these vaccines for their sustained use in South Korea. The present study provides comprehensive and up-to-date information regarding the epidemiology, genetic diversity, and evolution of the circulating rotaviruses in South Korea.
- Published
- 2014
7. Peptoniphilus rhinitidis sp. nov., isolated from specimens of chronic rhinosinusitis
- Author
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Jin Yeul Ma, Min Young Jung, In-Soon Park, Wonyong Kim, Jae Hoon Cho, Joong-Su Kim, Soo-Je Park, Yeseul Shin, Jayoung Paek, and Young-Hyo Chang
- Subjects
Male ,food.ingredient ,Molecular Sequence Data ,Microbial Sensitivity Tests ,Gram-Positive Bacteria ,Peptoniphilus ,DNA, Ribosomal ,Microbiology ,food ,RNA, Ribosomal, 16S ,Genotype ,otorhinolaryngologic diseases ,Cluster Analysis ,Humans ,Sinusitis ,Gene ,Gram-Positive Bacterial Infections ,Phylogeny ,Rhinitis ,biology ,Clostridiales ,Fatty Acids ,Sequence Analysis, DNA ,Ribosomal RNA ,biology.organism_classification ,16S ribosomal RNA ,Incertae sedis ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Infectious Diseases ,Chronic Disease ,Bacteria - Abstract
Chronic rhinosinusitis (CRS) is an inflammatory disorder of the nasal cavity and paranasal sinus related to bacterial infection. A previous study suggested that a specific bacterial group may have an important role in the course of CRS. In this study, bacteria isolated from CRS patients were characterized. A total of 15 strains were identified as Gram-positive anaerobic cocci (GPAC), which were able to utilize peptone as a sole carbon source. Sequencing of the 16S ribosomal RNA gene revealed that the isolates were closely related to members of the genus Peptoniphilus (>97% similarity) within the Clostridiales Family XI. Incertae Sedis. Genotypic and phenotypic characterization suggests that these isolates represent a novel species of the genus Peptoniphilus associated with CRS. The type strain of Peptoniphilus rhinitidis is 1-13T (=KCTC 5985T=JCM 17448T).
- Published
- 2014
8. Expression and immunogenic analysis of recombinant polypeptides derived from capsid protein VP1 for developing subunit vaccine material against hepatitis A virus
- Author
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Jong-Hwa Park, Dae Kyun Chung, Hyun Ho Lee, Wonyong Kim, In-Sik Chung, and Kyoung Ok Jang
- Subjects
Antigenicity ,viruses ,Protein subunit ,Antibodies, Viral ,medicine.disease_cause ,law.invention ,Interferon-gamma ,Mice ,Affinity chromatography ,law ,Escherichia coli ,medicine ,Animals ,Cloning, Molecular ,Cells, Cultured ,Viral Structural Proteins ,Mice, Inbred BALB C ,biology ,Molecular mass ,Interleukin-6 ,Chemistry ,virus diseases ,Viral Vaccines ,Hepatitis A ,biochemical phenomena, metabolism, and nutrition ,Virology ,Molecular biology ,Recombinant Proteins ,Capsid ,Immunoglobulin G ,biology.protein ,Recombinant DNA ,Capsid Proteins ,Female ,Immunization ,Hepatitis A virus ,Antibody ,Spleen ,Biotechnology - Abstract
Three recombinant polypeptides, VP1-His, VP1-3N-His, and 3D2-His, were produced by Escherichia coli expression system. Recombinant VP1-His, VP1-3N-His, and 3D2-His were expressed as bands with molecular weights of 32, 38, and 30 kDa, respectively. These were purified by affinity chromatography using Ni-NTA Fast-flow resin and/or ion-exchange chromatography using DEAE-Sepharose Fast-flow resin. Intraperitoneal immunizations of recombinant polypeptides successfully elicited the productions of VP1-His, VP1-3N-His, and 3D2-His specific IgG antibodies (IgG subclass distribution of IgG1>IgG2a>IgG2b>IgG3) in sera and induced the secretions of cytokines IFN-γ and IL-6 in spleen cells. Sera from recombinant VP1-His-, VP1-3N-His-, and 3D2-His-immunized mice neutralized the propagation of HAV. The highest neutralizing activity was shown in sera from recombinant VP1-3N-His-immunized mice. These results suggest that recombinant VP1-3N-His can be a useful source for developing hepatitis A virus (HAV) subunit vaccine candidates.
- Published
- 2014
9. Beta-catenin promoter polymorphism is associated with asthma risk in Korean subjects
- Author
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Byoung Whui Choi, Sang-In Chung, Seong Jun Seo, Yoosik Yoon, Kijeong Kim, Hyeong-Kyu Lee, Seok-min Kim, Sungmin Bae, Dong-Seok Kim, Wonyong Kim, and Haeyong Lee
- Subjects
Adult ,Male ,Risk ,medicine.medical_specialty ,Clinical Biochemistry ,Gene Expression ,Single-nucleotide polymorphism ,Polymorphism, Single Nucleotide ,Internal medicine ,Genotype ,Humans ,Medicine ,SNP ,Promoter Regions, Genetic ,Gene ,Genetic Association Studies ,beta Catenin ,Aged ,Asthma ,Genetics ,Korea ,business.industry ,Haplotype ,Promoter ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,Logistic Models ,Endocrinology ,Haplotypes ,Case-Control Studies ,Female ,business - Abstract
Objectives The effects of β-catenin promoter haplotypes on its mRNA expression levels and asthma risks were investigated in Korean subjects. Design and methods The genotype analyses were conducted by a Taqman method for 684 Korean subjects, 400 controls and 284 with asthma. Measurement of mRNA expression levels in peripheral blood nucleated cells were conducted on subjects whose buffy coat fractions were available (n = 185). Logistic regression analyses were conducted to test the associations of the β-catenin promoter haplotypes with asthma risks. Results Four SNPs, − 10,288C>T (rs7630377), − 6,426C>G (rs9859392), − 4,361G>C (rs9870255), and − 765G>A (rs3864004), were identified in the promoter region of the β-catenin gene, and three common haplotypes were constructed from them. Haplotype ht1[CCGG] was associated with decreased β-catenin mRNA expression levels and a lower asthma risk with an odds ratio of 0.53, while ht2[TGCA] was associated with increased mRNA expression levels and a higher asthma risk with an odds ratio of 2.34. Ht3[TCGG] had no significant effects on both. Conclusions Our findings show that β-catenin promoter polymorphism affects its mRNA expression levels, and also is significantly associated with the asthma risk of Korean subjects.
- Published
- 2012
10. Guanylate cyclase activator YC-1 potentiates apoptotic effect of licochalcone A on human epithelial ovarian carcinoma cells via activation of death receptor and mitochondrial pathways
- Author
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Chung Soo Lee, Sang Won Kwak, Jeong Jae Lee, Min Sung Lee, Wonyong Kim, Yun Jeong Kim, Eon Sob Park, Soon Chul Myung, and Seon Ae Lee
- Subjects
Programmed cell death ,Indazoles ,Licochalcone A ,Poly (ADP-Ribose) Polymerase-1 ,Apoptosis ,Carcinoma, Ovarian Epithelial ,chemistry.chemical_compound ,Chalcones ,Cell Line, Tumor ,Ovarian carcinoma ,Survivin ,Humans ,Drug Interactions ,Neoplasms, Glandular and Epithelial ,Caspase ,Cell Nucleus ,Ovarian Neoplasms ,Pharmacology ,biology ,Carcinoma ,Antineoplastic Agents, Phytogenic ,Neoplasm Proteins ,Cell biology ,Enzyme Activation ,chemistry ,Guanylate Cyclase ,Cell culture ,Caspases ,Proteolysis ,Cancer cell ,biology.protein ,Cancer research ,Female ,Poly(ADP-ribose) Polymerases ,Tumor Suppressor Protein p53 ,Apoptosis Regulatory Proteins ,Signal Transduction - Abstract
Natural phenol licorice compounds have been shown to induce apoptosis in cancer cells. 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1) may enhance the sensitivity of cancer cells to anticancer drugs. However, the combined effect of licochalcone A and YC-1 on cell death in ovarian cancer cells has not been studied. We assessed the combined effect of licochalcone A and YC-1 on apoptosis in human epithelial ovarian carcinoma cell lines in relation to the cell death process. In the OVCAR-3 and SK-OV-3 cell lines, licochalocone A induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels; an increase in Bax levels; loss of the mitochondrial transmembrane potential; cytochrome c release; activation of caspases (-8, -9 and -3); cleavage of PARP-1; and an increase in the tumor suppressor p53 levels. YC-1 enhanced licochalcone A-induced apoptosis-related protein activation, nuclear damage and cell death. These results suggest that YC-1 may potentiate the apoptotic effect of licochalcone A on ovarian carcinoma cell lines by increasing the activation of the caspase-8- and Bid-dependent pathway and the mitochondria-mediated apoptotic pathway, leading to caspase activation. The combination of licochalcone A and YC-1 may confer a benefit in the treatment of human epithelial ovarian adenocarcinoma.
- Published
- 2012
11. Prostate Specific Antigen Velocity per Prostate Volume: A Novel Tool for Prostate Biopsy Prediction
- Author
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Se Young Choi, Wonyong Kim, Young Sun Kim, Soon Chul Myung, In Ho Chang, and Tae-Hyoung Kim
- Subjects
Adult ,Male ,Oncology ,medicine.medical_specialty ,Prostate biopsy ,Biopsy ,Urology ,Prostate cancer ,Prostate ,Internal medicine ,Odds Ratio ,medicine ,Humans ,Ultrasound, High-Intensity Focused, Transrectal ,Aged ,Aged, 80 and over ,PSA Velocity ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Prostatic Neoplasms ,Middle Aged ,Prostate-Specific Antigen ,medicine.disease ,Prostate-specific antigen ,Treatment Outcome ,medicine.anatomical_structure ,ROC Curve ,Quartile ,Multivariate Analysis ,Transrectal ultrasonography ,business - Abstract
Objective To investigate whether altered prostate specific antigen (PSA) levels due to individual prostate growth may affect the PSA velocity (PSAV). Materials and Methods Between January 2000 and December 2009, a total of 159 men with at least 2 transrectal ultrasonography (TRUS) procedures and concurrent PSA measurements underwent prostate biopsy because of suspicion of prostate cancer. We measured PSAV, prostate volume velocity (PVV), PSA density (PSAD), PSAD velocity (PSADV), and PSAV per initial volume. We then classified the total group into a prostate cancer (PC) group and non-PC group, and compared the 2 groups. We investigated which variables were exact to predict prostate biopsy using univariate and multivariate analyses, and assessed the diagnostic performance using the receiver operating characteristic (ROC) curve. Results PVV showed a positive correlation with initial prostate volume in the total and non-PC group; PVV showed a positive correlation with PSAV, and initial prostate volume correlated with PSAV in the non-PC group. The PC group showed smaller prostate volumes, higher PSAD, higher PSADV, higher PSAV per initial volume, and longer follow-up periods. After adjusting for confounding factors, the odds ratios of prostate cancer across the quartile of PSAVD were 1, 1.889, 3.226, and 7.125 ( P for trend=.007 ), and PSAV per initial volume were 1, 2.924, 2.937, and 7.536 ( P for trend=.031 ). On the ROC curve, the areas under the curves (AUC) of PSAV per initial volume were higher than for PSAV and PSADV. Conclusion Altered PSA levels due to individual prostate growth may affect the use of PSAV to predict prostate biopsy outcomes in follow-up.
- Published
- 2011
12. Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation
- Author
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Hunseung Kang, Hyun Ju Jung, Joo Yeol Kim, Yeon Soo Han, Seung Han Oh, and Wonyong Kim
- Subjects
Cytoplasm ,Physiology ,Arabidopsis ,Gene Expression ,RNA-binding protein ,Plant Science ,Genes, Plant ,Stress, Physiological ,Escherichia coli ,Genetics ,medicine ,Cellular localization ,Cell Nucleus ,Zinc finger ,Base Sequence ,biology ,Arabidopsis Proteins ,Nucleotides ,RNA-Binding Proteins ,RNA ,Zinc Fingers ,Cold-shock domain ,biology.organism_classification ,Adaptation, Physiological ,Cold Temperature ,Complementation ,Biochemistry ,Mutagenesis ,Seedlings ,Mutation ,Seeds ,Cold Shock Proteins and Peptides ,Cold sensitivity ,medicine.symptom - Abstract
Among the three zinc finger-containing glycine-rich RNA-binding proteins, named AtRZ-1a, AtRZ-1b, and AtRZ-1c, in the Arabidopsis thaliana genome, AtRZ-1a has previously been shown to enhance cold and freezing tolerance in Arabidopsis. Here, we determined and compared the functional roles of AtRZ-1b and AtRZ-1c in Arabidopsis and Escherichia coli under cold stress conditions. AtRZ-1b, but not AtRZ-1c, successfully complemented the cold sensitivity of E. coli BX04 mutant cells lacking four cold shock proteins. Domain deletion and site-directed mutagenesis showed that the zinc finger motif of AtRZ-1b is important for its complementation ability, and that the truncated N- and C-terminal domains of AtRZ-1b and AtRZ-1c harbor the complementation ability. Despite an increase in transcript levels of AtRZ-1b and AtRZ-1c under cold stress, overexpression or loss-of-function mutations did not affect seed germination or seedling growth of Arabidopsis under cold stress conditions. AtRZ-1b and AtRZ-1c proteins, being localized to the nucleus, have been shown to bind non-specifically to RNA sequences in vitro, in comparison to AtRZ-1a that is localized to both the nucleus and the cytoplasm and binds preferentially to G- or U-rich RNA sequences. Taken together, these results demonstrate that the three AtRZ-1 family members showing different cellular localization and characteristic nucleic acid-binding property have a potential to contribute differently to the enhancement of cold tolerance in Arabidopsis and E. coli.
- Published
- 2010
13. β-Catenin mediates the anti-adipogenic effect of baicalin
- Author
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Sang-In Chung, Wonyong Kim, Haeyong Lee, Kijeong Kim, Sungmin Bae, and Yoosik Yoon
- Subjects
medicine.medical_specialty ,Biophysics ,Peroxisome proliferator-activated receptor ,Biology ,Biochemistry ,Fatty acid-binding protein ,Mice ,chemistry.chemical_compound ,3T3-L1 Cells ,Internal medicine ,Enhancer binding ,CCAAT-Enhancer-Binding Protein-alpha ,medicine ,Animals ,Molecular Biology ,beta Catenin ,Flavonoids ,chemistry.chemical_classification ,Lipoprotein lipase ,Adipogenesis ,Wnt signaling pathway ,Cell Biology ,Cell biology ,PPAR gamma ,Wnt Proteins ,Endocrinology ,chemistry ,Low-density lipoprotein ,Baicalin - Abstract
beta-Catenin reportedly inhibits adipogenesis through the down-regulations of peroxisome proliferator-activated receptor (PPAR)gamma and CCAAT/enhancer binding protein (C/EBP)alpha. We report that baicalin, a natural flavonoid compound, inhibits adipogenesis by modulating beta-Catenin. During 3T3-L1 cell adipogenesis, beta-Catenin was down-regulated, but baicalin treatment maintained beta-Catenin expression. Anti-adipogenic effects of baicalin were significantly attenuated by beta-Catenin siRNA transfection. beta-Catenin siRNA rescued the reduced expressions of PPARgamma, C/EBPalpha, fatty acid binding protein 4 and lipoprotein lipase by baicalin. Furthermore, baicalin modulated members of the WNT/beta-Catenin pathway by maintaining the expressions of low-density lipoprotein receptor-related protein 6, disheveled (DVL)2 and DVL3. These findings suggest that beta-Catenin mediates the anti-adipogenic effects of baicalin.
- Published
- 2010
14. Characterization of BNT2, an intrinsically curved DNA of Escherichia coli O157:H7
- Author
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Moon Kyoo Park, Wonyong Kim, Jang Won Yoon, Mi-Sun Park, Jong-Chul Kim, Carolyn J. Hovde, and Seung-Hak Cho
- Subjects
DNA, Bacterial ,Operon ,Biophysics ,Virulence ,Biology ,Escherichia coli O157 ,medicine.disease_cause ,Biochemistry ,Article ,chemistry.chemical_compound ,Plasmid ,medicine ,Promoter Regions, Genetic ,Molecular Biology ,Escherichia coli ,Regulation of gene expression ,Base Sequence ,Gene Expression Regulation, Bacterial ,Cell Biology ,Spacer DNA ,Molecular biology ,Thymine ,chemistry ,Mutagenesis, Site-Directed ,Nucleic Acid Conformation ,DNA - Abstract
The gene regulation by intrinsically curved DNA is one way for bacterial sensing of and response to environmental changes. Previously, we showed that the genetic element BNT2 upstream of the ecf ( eae -positive conserved fragment) operon in the Escherichia coli O157:H7 virulence plasmid (pO157) has characteristics typical of intrinsically curved DNA, including the presence of multi-homopolymeric adenine:thymine tracts (AT tracts) and electrophoretic anomaly at 4 °C. Here we report that a local intrinsic curvature induced by the two phased AT tracts within the unusual promoter sequence of BNT2 played a major role for its temperature-dependent promoter activity. The base substitution of the AT tract in the spacer DNA between the −35 and the unusual −10 regions of the BNT2 promoter with non-AT tract sequence reduced intrinsic curvature slightly at 4 °C, but greatly affected its transcriptional activity. This implies that such a local intrinsic curvature within the unusual promoter of BNT2 is important for thermoregulation of the ecf operon.
- Published
- 2010
15. Tsukamurella spumae sp. nov., A Novel Actinomycete Associated with Foaming in Activated Sludge Plants
- Author
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Sun-Woo Nam, Wonyong Kim, Seung Bum Kim, Jongsik Chun, Michael Goodfellow, and Jolanta Zakrzewska-Czerwińska
- Subjects
Tsukamurella ,Molecular Sequence Data ,Microbial Sensitivity Tests ,medicine.disease_cause ,Ribotyping ,Applied Microbiology and Biotechnology ,Microbiology ,RNA, Ribosomal, 16S ,Tsukamurella spumae ,Actinomycetales ,Botany ,medicine ,Ribosomal DNA ,Ecology, Evolution, Behavior and Systematics ,Base Composition ,Base Sequence ,Sewage ,biology ,Nucleic Acid Hybridization ,biology.organism_classification ,16S ribosomal RNA ,Bacterial Typing Techniques ,Phenotype ,Taxon ,Taxonomy (biology) ,Bacteria - Abstract
Summary A polyphasic taxonomic study was undertaken to establish the taxonomic position of six representative strains isolated from activated sewage sludge foam. The organisms were found to have chemical and morphological properties consistent with their assignment to the genus Tsukamurella . DNA:DNA relatedness studies showed that five out of the six isolates formed a distinct genomic species, the remaining strain was most closely associated with this taxon. The five isolates had a unique phenotypic profile that served to distinguish them from representatives of the validly described species of Tsukamurella . The combination of the genotypic and phenotypic data indicated that the five strains should be classified as a new species in the genus Tsukamurella . The name proposed for this taxon is Tsukamurella spumae , the type strain is N1171 T (= DSM 44113 T = NCIMB 13947 T ). It was also shown that some of the reference strains were misclassified as Tsukamurella paurometabola .
- Published
- 2003
16. Prevalence and spread of integron–IS26 in imipenem-resistant Acinetobacter baumannii clinical isolates in South Korea
- Author
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Ji Young Ahn, Eui Chong Kim, Xue Min Li, Seong Geon Hong, Sun Hoe Koo, Geon Park, Won Keun Song, Jong Hee Shin, Sung Heui Shin, Ji Hyun Cho, Tae Yeal Choi, Sung Hee Lee, Kyungwon Lee, Young Uh, Young Jin Park, Jung Oak Kang, Wonyong Kim, Dong-Min Kim, Hye Soo Lee, Yeon Joon Park, Jong Wook Lee, Dae Soo Moon, Dongeun Yong, Chulhun L. Chang, Sook Jin Jang, Wee Gyo Lee, Ok Yeon Jeoung, and Seok Jeong
- Subjects
Acinetobacter baumannii ,Microbiology (medical) ,Imipenem ,medicine.drug_class ,Antibiotics ,Microbial Sensitivity Tests ,Drug resistance ,Integron ,Integrons ,Microbiology ,Drug Resistance, Bacterial ,Republic of Korea ,Prevalence ,medicine ,Humans ,Pharmacology (medical) ,biology ,General Medicine ,biology.organism_classification ,Anti-Bacterial Agents ,Infectious Diseases ,DNA Transposable Elements ,biology.protein ,Acinetobacter Infections ,medicine.drug ,Beta lactam antibiotics - Published
- 2009
17. Transgenic expression of rotavirus capsid protein (VP7) in alfalfa for edible vaccine
- Author
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Kyeong Ryeol Lee, Kyung Hee Roh, In Sig Chung, Jong Bum Kim, Jong Hwa Park, Eun Young Lee, Sun Hee Kim, Wonyong Kim, and Hyun Uk Kim
- Subjects
Capsid ,Transgene ,Rotavirus ,medicine ,Bioengineering ,General Medicine ,Biology ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Virology ,Biotechnology - Published
- 2014
18. HNF1B Polymorphism Associated With Development of Prostate Cancer in Korean Patients
- Author
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June Hyun Han, Soon Chul Myung, Joon Seol Bae, Kyung Do Kim, In Ho Chang, Jaehyouk Lee, Shin Young Lee, Hyoung Doo Shin, Wonyong Kim, and Hae Jong Kim
- Subjects
Male ,Risk ,Oncology ,medicine.medical_specialty ,Urology ,Single-nucleotide polymorphism ,Disease ,Lower risk ,Polymorphism, Single Nucleotide ,Prostate cancer ,Internal medicine ,Odds Ratio ,Humans ,Medicine ,Aged ,Hepatocyte Nuclear Factor 1-beta ,Genetic association ,Aged, 80 and over ,Korea ,Polymorphism, Genetic ,business.industry ,Haplotype ,Prostatic Neoplasms ,Odds ratio ,Middle Aged ,HNF1B ,medicine.disease ,Haplotypes ,Case-Control Studies ,Regression Analysis ,business - Abstract
Objective To identify whether the genetic variations in HNF1B are associated with the development of prostate cancer in Korean patients. Genome-wide association studies have found the HNF1B gene at 17q12 to be a major causal gene for the risk of prostate cancer. Methods We evaluated the association of 47 single nucleotide polymorphisms (SNPs) in the HNF1B gene with prostate cancer risk and clinical characteristics (Gleason score and tumor stage) in Korean men (240 case subjects and 223 control subjects) using unconditional logistic regression analysis. Results Of the 47 SNPs, 14 were associated with prostate cancer risk ( P = .002-.02); 9 SNPs were associated with a lower risk of prostate cancer (odds ratio 0.67-0.71, P = .005-.05), and 5 SNPs were associated with a greater risk of disease (odds ratio 1.49-1.51, P = .002-.02). In an analysis involving only patients with prostate cancer, 1 SNP (rs11868513) in the HNF1B gene was more frequent in patients with tumors with a greater stage than in those with a lower tumor stage. Two SNPs (rs4430796 and rs2074429) and 1 haplotype ( Block3_ht1 ) were more frequent in patients with Gleason score of ≥7 than in those with Gleason score Conclusion As in studies from other populations, our findings indicate that HNF1B is also associated with prostate cancer risk in the Korean population.
- Published
- 2011
19. Development of transgenic plants expressing hepatitis A virus VP1
- Author
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Kyung Il Kim, Dong-Hwa Shon, In-Sik Chung, Jeon Hwang-Bo, Ha-Young Chung, Wonyong Kim, Ho-Yong Chung, K. H. Yoo, and Jong-Bum Kim
- Subjects
Bioengineering ,Genetically modified crops ,Biology ,Applied Microbiology and Biotechnology ,Virology ,Hepatitis a virus ,Biotechnology - Published
- 2009
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