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Objective This study aimed to explore the effects of lentivirus-mediated ornithine decarboxylase (ODC) gene on the proliferation and apoptosis of fibroblast-like synoviocytes (FLSs) in rats with rheumatoid arthritis (RA). Methods Twenty Lewis rats were randomized into control group (ten rats without processing) and RA group (ten rats of adjuvant-induced arthritis). The third-generation FLSs were randomized into test, control and blank groups. MTT assay and flow cytometry were employed to detect cell proliferation and apoptosis, respectively. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of tumor necrosis factor α (TNF-α), interferon-γ (IFN-γ), and interleukin-2 (IL-2). Results Lewis rats in the RA group became ill from 11 days on and got seriously ill 18 days after modeling. However, rats in the control group had no obvious change. MTT assay showed that the test group had higher cell proliferation than the blank and control groups ( P 1 P 2 P 1 P 2 P P > 0.05). Conclusion The results indicated that overexpression of ODC gene promotes the proliferation while suppressing apoptosis of FLSs in rats with RA.

This paper provides a method of using multi-channel Phase Diversity technique for online calibration of non-common path aberration of AO system. The PD technique estimate the phase aberration by collecting one or more frames images with short time of exposure at focal plane and out-of-focus plane, meanwhile reconstruct the target image. Under the working condition of closed-loop AO system, the static aberration of imaging path can be detected online by PD algorithm and transfer the wavefront aberration coefficient into the initial form of the deformable mirror, thus compensate for the static aberration of the non-common optical path. The experiment result shows that the imaging quality is improved by a large margin after calibration, the FWHM of the point source target decreased from 8.90 to 7.64; the wavefront RMS of imaging path decreased from 0.133 λ to 0.033 λ, and PV deceased from 0.79 λ to 0.17 λ, in accordance with the wavefront aberration measured by the closed-loop. This fully testified the ability of the application in optical detection of the Phase Diversity. There is no need to modify the original adaptive optical path, and it can precisely solve the system aberration in the condition of high SNR, and is one of the ideal optical detection techniques of large-diameter optical imaging system.