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Inulin, as a dietary fiber, exerted prominent anti-obesity effects by modulating gut microbiota. However, the possible relationship and interplay of gut microbiome and function of distal intestine is still unclear now. This study aimed to investigate the possible targets of microbes and the related intestinal genes mediated by inulin. C57 BL/6 male mice were randomly allocated to chow diet (Chow) group, high-fat diet (HFD) group, and HFD supplemented with 3 % inulin (Inulin) group. Compared with HFD treatment, inulin supplementation significantly decreased the body weight, fat deposition, and fasting blood glucose level. In addition, mice treated with inulin had a remarkable alteration in the structure of cecal microbiota and transcriptomic profiling of ileum. In particular, inulin supplementation significantly reversed the HFD induced expression of Bacteroides, Allobaculum and nonrank_f_Bacteroidates_S24-7_group, and reversed the expression of genes belonging to phospholipase A2 (PLA2) family and cytochrome P450 (CYP450) family. In summary, inulin might alleviate HFD-induced fat deposition and metabolic disorders via regulating lipid metabolism of ileum, while the interaction between the sPLA2s and gut microbes might play important roles in the process.

Methicillin-resistant Staphylococcus aureus (MRSA) has become the most important pathogen of hospital-acquired (HA) or community-acquired (CA) infections. However, it is unclear of the cytokines responsible for pathological hyper-inflammation in sepsis related cytokine storm for MRSA infection. In this study, we selected typical HA-MRSA strain (YNSA163: ST239-t030-SCCmecⅢ) and two CA-MRSA isolates (YNSA7: ST59-t439-SCCmecⅣa and YNSA53: ST59-t437-SCCmecⅤb) from our previous research, infected on BALB/c mice, and analyzed the cytokine storm patterns during infection process. The animal experiments revealed the most serious lethal effect on BALB/c mice caused by YNSA7 strain infection, followed by YNSA53, and no BALB/c mice died for YNSA163 infection. Histopathological analyses revealed that lung was the most seriously damaged organs, followed by spleen and kidney, especially for CA-MRSA infection. The severe inflammatory reactions, tissue destruction, and massive exudation of inflammatory mediators and cells could be identified in CA-MRSA strains infected mice. Interleukin-6 (IL-6) and IL-10 were both highly expressed in spleen and lung of YNSA7 and YNSA53 dead cases compared with YNSA53 survived and YNSA163 cases, which demonstrated cytokine storm pattern for CA-MRSA strains infection. The results of IL-6 intervention experiment verified that the enhanced IL-6 secretion was responsible for the host lethality of YNSA7 infection. RNA-sequencing results among three MRSA isolates indicated most of the differentially expressed genes referred to cellular process, metabolism and genetic information processing of bacteria. Specifically, clpP, chp chemotaxis inhibit, fnbB, pathogencity island protein and virulence associated protein E were highly expressed in YNSA7 strain. In general, CA-MRSA strains provoked cytokine storm on BALB/c mice led to severe infection and lethality, the up-regulated of some virulence genes might play important role in pathogenesis.

The Cuonadong deposit is located in southern Tibet and represents the first large-scale tin polymetallic deposit in the Himalayan region. Sn-(W) skarn mineralization is spatially related to leucogranites, whereas Be-Nb-Ta mineralization mainly develops in pegmatites. Alteration and veining in this deposit can be divided in to five stages: prograde skarn (stage I), retrograde skarn (stage II), cassiterite-quartz vein (stage III), cassiterite-sulfide vein (stage IV) and fluorite quartz vein (stage V). 40Ar 39Ar dating of muscovite from the skarn and phlogopite from the cassiterite-sulfide vein yield isochron ages of 15.4 ± 0.3 Ma and 15.0 ± 0.3 Ma, respectively, whereas U Pb dating of cassiterite from the skarn yields a Tera-Wasserburg low-intercept precise age of 14.2 ± 0.2 Ma. Zircon and monazite U Pb ages of the causative stanniferous leucogranites are 15.3 ± 0.1 Ma and 14.9 ± 0.2 Ma, respectively. Mineralization ages are consistent with the emplaced ages of the granites within the analytical uncertainties, which indicates that the tin polymetallic mineralization is genetically related to the Miocene leucogranites. Sn-bearing leucogranites have zircon eHf(t) values that vary from −13.3 to −8.5 (-10.7 on average), with an average TDM2 value of 1.56 Ga, which reveals that the leucogranites are derived from partial melting of ancient metasedimentary rocks. According to the zircon trace elements, the Miocene leucogranites are highly differentiated reduced S-type granites that formed at relatively high temperature. The average homogenization temperatures of fluid inclusions in different minerals that formed at stages II, III, IV and V are 351, 315, 240 and 175 °C, respectively, whereas their salinities are 8.3, 4.9, 9.5 and 3.8 wt% NaCl equiv., respectively. The C-H-O-S-Pb isotopes indicate that the ore-forming fluid and materials mainly originated from Miocene leucogranite. Dehydration and partial melting of mica in the Greater Himalayan crystalline complex due to east-west extension at 18–14 Ma developed the stanniferous leucogranite and ore-controlling fault system. Because of the pervasive occurrence of gneiss domes and Miocene Sn-bearing leucogranites similar to Cuonadong, the Himalaya has strong potential to be a new globally important Sn-(W) rare metal metallogenic belt.

Background Maternal stress and nutrition during pregnancy are two of the most commonly studied factors in the context of fetal development. However, few investigators have considered the combined effects of stress and diet in pregnant women and their offspring. Aims To determine the impact of maternal stress on offspring birth weight, and the potential mediating role of maternal dietary patterns. Methods A total of 3542 pregnant women and their singleton live births were recruited from Shanghai Maternal-Child Pairs Cohort (Shanghai MCPC). Maternal stress was assessed using the Life Event Scale for Pregnant Women (LESPW) in early and late pregnancy. Food frequency questionnaire (FFQ) was performed to evaluate maternal diet in late pregnancy. Multiple linear regression was conducted to estimate the associations between maternal stress and child birth weight. Logistic regression was performed to calculate the association between maternal stress and small for gestational age (SGA)/large for gestational age (LGA). The bootstrap method was used to investigate the mediating effects of maternal dietary patterns. Results Maternal subjective events stress (β = 0.367) and total stress (β = 0.079) in early pregnancy, and positive objective life events stress (β = 0.275) in late pregnancy, were positively associated with birth weight. Maternal dietary pattern of “high-fat and sugar” mediated the associations between subjective events stress and total stress during early pregnancy and birth weight. An increased risk for LGA was observed among women exposed to relatively higher stress during early pregnancy (OR, 1.416; 95% CI, 1.035-1.937). No statistically significant associations were found between maternal stress during late pregnancy and child birth weight or SGA/LGA. Conclusions Maternal life events stress during early pregnancy significantly increases neonatal birth weight and risk for LGA, which may mediate by a “high-fat and sugar” dietary pattern. Limitations The lack of biological markers limits the explanation of the mechanism. The assessment of diet used food intake frequency to evaluate dietary characteristics, which may miss information of energy intake.

Background Recent research suggested that oxytocin might be associated with prenatal anxiety or depression. Also, our previous study indicated that resilience could buffer the effect of stress on prenatal anxiety and depression, which is a kind of psychological adjustment ability to stress in life. The effects of oxytocin and resilience are supposed to be similar but no study was carried out to find the association between them. Current evidence on the effect of oxytocin on prenatal mental health were with small sample size and equivocal results, especially with a lack of studies in Chinese pregnant women. Aims To investigate the relationship between oxytocin and resilience, and its role in regulating prenatal anxiety and depression among Chinese women. Methods The study was based on a sub-sample of the Shanghai Maternal-Child Pairs Cohort (Shanghai MCPC) by stratified random sampling. A total of 632 participants completed blood collection for serum oxytocin measurement and questionnaires pertaining to early pregnancy stress and resilience in the second trimester, which were assessed by the Life Event Scale for Pregnancy Women (LESPW) and the revised Resilience Scale for Adults (RSA), respectively. Prenatal anxiety and depression were assessed by Self-Rating Anxiety Scale (SAS) and the Center for Epidemiological Survey, Depression Scale (CES-D), respectively, at the third trimester. Correlation analysis and stratified linear regression analyses were conducted to understand the role of oxytocin in regulating prenatal anxiety and depression. Results The concentration of serum oxytocin in the second trimester ranged from 98.14 pg⋅ml−1 to 197.28 pg⋅ml−1, and there was no significant difference between different gestational age (r = 0.024, P = 0.579). The association between stress and prenatal anxiety was stronger in high oxytocin group than that in low oxytocin group, with B value of 3.338 and 2.638, respectively, after adjusting for residence, educational level, family income and parity. The same effect was found in the association between stress and prenatal depression, with B value of 2.360 and 2.060, respectively. However, it was not significantly associated with early pregnancy stress (Z = 1.933, P = 0.630), prenatal anxiety (Z = 1.387, P = 0.165), prenatal depression (Z = −0.027, P = 0.978), nor resilience (F = 1.282, P = 0.280). Limitations The existence of recall bias and restricted residential areas of the participants may limit the extrapolation of the study. Also, unsynchronized sampling timing of the oxytocin and psychological resilience measures is another limitation. Conclusions Serum oxytocin levels in the second trimester had an effect of modification between early pregnancy stress and prenatal anxiety and depression. Pregnant women with high oxytocin in the second trimester should receive more attention and further study is needed on the mechanism of oxytocin for the mental health of pregnant women.

Resveratrol is a dietary polyphenol that interacts with gut microbiota to possess various biological activities. To identify the microbial metabolites of resveratrol, fresh feces from 12 volunteers were cultured in vitro. Their urine samples were collected after taking a commercial capsule containing 600 mg of resveratrol. Metabolites were characterized and quantified by UPLC-Q-Exactive plus orbitrap MS/MS. The results showed that dihydroresveratrol, 3-(4-hydroxyphenyl)-propionic acid, and lunularin were the major microbial metabolites of RSV with interindividual differences. 3-(4-Hydroxyphenyl)-propionic acid significantly attenuated the inflammatory response of LPS-treated RAW264.7 cells and DSS-induced colitis in antibiotics-treated pseudo-germ-free mice by regulating MAPK and NF-κB pathways. In contrast, dihydroresveratrol did not exhibit significant anti-inflammatory effects, and lunularin exhibited pro-inflammatory effects in cells. This study may help to better understand the health effects of resveratrol and its microbial metabolites.

Associations between individual exposure to ozone (OWe aimed to explore the associations of gestational OA total of 7834 pregnant women were included. Individual ONearly 13.29 % of participants developed GDM. After controlling for covariates, we observed increased GDM risks per IQR increment of OIn summary, we found that gestational O

Prebiotics are effective in modulating gut microbiota and may further benefit colitis remission. 2'-fucosyllactose (2'FL) and galactooligosaccharide (GOS) are the main prebiotics in human and animal milk, respectively. This study aimed to investigate the colitis-preventing effects of 2'FL and GOS, and explore the underlying mechanisms involving the gut microbiota. The chronic colitis was induced by 1.5 % dextran sulfate sodium (DSS) for 4 consecutive cycles and manifested as aggravation of colitis symptoms, gut barrier disruption, and colonic inflammation. We found that 2'FL was more effective than GOS against colitis at the same dosage (500 mg/kg bw). 2'FL and GOS have a differential response on gut microbiota, reflecting the inhibition of Romboutsia and the enrichment of Akkermansia, Bifidobacterium, Faecalibaculum, and unclassified_f_Lachnosipiaceae. In addition, the differential response on microbial metabolome was reflected in the elevation of secondary bile acids, which activated Takeda G protein-coupled receptor 5 (TGR5) and further suppressed the nuclear factor-κB (NF-κB) pathway. Furthermore, fecal bacterial transplantation confirmed the critical role of gut microbiota in the prevention of colitis by 2'FL and GOS. Overall, microbiota and microbial metabolites are essential parts of 2'FL and GOS against colitis, and their differential responses may account for different effects in alleviating colitis.

Prenatal fine particulate matter (PMWe included 267 mother-child pairs in this analysis. Prenatal PMThe PMPrenatal exposure to PM

Ethylhexyl methoxycinnamate (EHMC) is one of the most frequently used UV filters in sunscreens and other cosmetic products. Its ubiquitous presence in various environmental matrices and its endocrine disrupting properties have been widely reported. However, we know little about the effect of EHMC exposure on humans, mainly due to its fast metabolism. In this study, urine and plasma of EHMC-dosed rats were analysed to identify its major metabolites. Five metabolites were found, with four firstly reported. Two metabolites were putatively identified as 4-methoxycinnamic acid (4-MCA) and 4'-methoxyacetophenone (4'-MAP). Quantitative results revealed that their excretion concentrations were much higher than the parent compound. Because of these high concentrations, for the human biomonitoring study, EHMC and these two metabolites were detected simultaneously in urine samples from Chinese children and adolescents. The results indicated wide exposure to EHMC, 4-MCA and 4'-MAP. The correlation between urinary concentration of EHMC and 4-MCA as well as 4-MCA and 4'-MAP provided important clues as to the sources and metabolic pathways among these three compounds. Several demographic factors were also assessed with the exposure level. As the first human exposure study of EHMC in a Chinese population, this report would help to establish an exposure database facilitating health risk assessment of EHMC.

Increasing evidence supports that maternal exposure to vanadium (V) is associated with adverse birth outcomes including preterm birth and low birth weight. However, the effect of V exposure on intrauterine fetal growth and the underlying biological mechanism are still unclear. The present study includes 227 mother-infant pairs from the Shanghai Maternal-Child Pairs Cohort to assess the gender-specific effect of intrauterine V exposure on fetal growth and related cytokines. Maternal blood samples were collected to measure V concentration and biomarkers of growth. We used multiple linear regression to evaluate the gender-specific effect of prenatal V exposure on birth parameter and growth-related cytokines. Mixed-effect models were applied to assess the non-linear association between gestational V exposure and intrauterine fetal growth. Covariates adjusted in the regression models as potential confounders including maternal age, pre-pregnancy body mass index, gestational weeks, parity, socio-demographic status, etc. Results showed that prenatal V exposure was negatively associated with birth weight (β = −64.73) in female newborns and body length (β = −0.10) in male. During the fetal period, maternal V exposure was associated with decreased biparietal diameter (β = −0.91), head circumference (β = −2.96), femur length (β = −0.72) and humerus length (β = −0.64) in male. Trimester-specific analyses showed that serum V concentration in the second trimester was associated with significant reductions in intrauterine growth parameters. Besides, prenatal V exposure could down-regulate the expression of growth hormone (GH) in both maternal blood (β = −0.23) and umbilical cord blood (β = −1.66) in male fetuses, and the expression of brain derived neurotrophic factor (BDNF) in cord blood in females (β = −0.52). Our results suggest that prenatal V exposure has a gender-specific effect on fetal growth and the second trimester may be a sensitive window. The disruption of grow-related cytokines may potentially be the biological mechanism of these effects.

Background It has been widely reported that gestational exposure to fine particulate matters (PM2.5) is associated with a series of adverse birth outcomes. However, the discrepancy between ambient PM2.5 concentrations and personal PM2.5 exposure would significantly affect the estimation of exposure-response relationship. Objective Our study aimed to predict gestational personal exposure to PM2.5 from the satellite-driven ambient concentrations and analyze the influence of other potential determinants. Method We collected 762 72-h personal exposure samples from a panel of 329 pregnant women in Shanghai, China as well as their time-activity patterns from Feb 2017 to Jun 2018. We established an ambient PM2.5 model based on MAIAC AOD at 1 km resolution, then used its output as a major predictor to develop a personal exposure model. Results Our ambient PM2.5 model yielded a cross-validation R2 of 0.96. Personal PM2.5 exposure levels were almost identical to the corresponding ambient concentrations. After adjusting for time-activity patterns and meteorological factors, our personal exposure has a CV R2 of 0.76. Conclusion We established a prediction model for gestational personal exposure to PM2.5 from satellite-based ambient concentrations and provided a methodological reference for further epidemiological studies.

The nature and evolution of the lithospheric mantle underlying Northeast (NE) China were investigated by assessing the mineral chemistry, water contents, and noble gas (He-Ar) isotopes of peridotite xenoliths captured by Cenozoic basalts from the Changbaishan and Longgang regions. The xenoliths, which have 863–1141 °C equilibration temperatures, primarily comprise spinel lherzolites and rare spinel harzburgites. The Mg# (Fo) values of olivine in the peridotite xenoliths vary from 86.9 to 91.3. The clinopyroxenes have high Ti/Eu and low (La/Yb)N, and their chondrite-normalized rare earth elements (REEs) exhibit light REE-depletion to -enrichment patterns, indicating that the mantle underneath the investigated region was predominantly subjected to partial melting (1%–10%) and was metasomatized by silicate melts. The measured 3He/4He ratios of the Changbaishan xenoliths have a narrow range from 5.8 Ra to 8.4 Ra with an average of 7.4 Ra. The 3He/4He isotopic ratios of the Longgang xenoliths varied from 4.7 Ra to 8.1 Ra with an average of 5.9 Ra; slightly lower than the Changbaishan xenoliths. The whole-rock H2O contents of the studied peridotite ranged from 9 to 132 ppm. The high H2O contents in excess of 50 ppm (up to 132 ppm) might represent newly accreted and cooled asthenospheric materials, while those with H2O contents lower than 50 ppm (as little as 9 ppm) may represent thinned, relic, ancient lithospheric mantle. These geochemical evidences, in combination with published data, indicated that the lithospheric mantle beneath the Changbaishan and Longgang in NE China is dominated by the younger and more fertile lithospheric mantle with a minor ancient and refractory keel. In addition, the lithospheric mantle of this area was metasomatized by melts related to the recent subduction event (e.g., Pacific oceanic plate). Therefore, the westward-dipping Pacific oceanic plate subduction had an important contribution to the transformation of the lithospheric mantle beneath NE China.

Ambient fine particulate matter (PM2.5) pollution has been implicated in the development of hypertensive disorders of pregnancy. However, evidence on the effects of PM2.5-derived chemical constituents on gestational blood pressure (BP) is limited, and the potential mechanisms underlying the association remain unclear. In this study, we repeated three consecutive 72-h personal air sampling and BP measurements in 215 pregnant women for 590 visits during pregnancy. Individual PM2.5 exposure level was assessed by gravimetric method and 28 PM2.5 chemical constituents were analyzed by ED-XRF method. Plasma biomarkers of endothelial function and inflammation were measured using multiplexed immunoassays. Robust multiple linear regression models were used to estimate the associations among personal PM2.5 exposure and chemical constituents, BP changes (compared with pre-pregnancy BP) and plasma biomarkers. Mediation analyses were performed to evaluate underlying potential pathways. Result showed that exposure to PM2.5 was significantly associated with increases in systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) in the early second trimester. Meanwhile, elevated concentration of lead (Pb) constituent in PM2.5 was significant associated with increases in DBP and MAP after adjusting for PM2.5 total mass. PM2.5 and Pb constituent also presented positive associations with plasma biomarkers of endothelial function (ET-1, E-selectin, ICAM-1) and inflammation (IL-1β, IL-6, TNFα) significantly. After multiple adjustment, elevated ET-1 and IL-6 were significantly correlated with increased gestational BP, and respectively mediated 1.24%-25.06% and 7.01%-10.69% of the increased BP due to PM2.5 and Pb constituent exposure. In conclusion, our results suggested that personal exposure to PM2.5 and Pb constituent were significantly associated with increased BP during pregnancy, and the early second trimester might be the sensitive window of PM2.5 exposure. The endothelial dysfunction and elevated inflammation partially mediated the effect of PM2.5 and Pb constituent on BP during pregnancy.

Background Prenatal anxiety/depression has been a major public health problem globally with higher prevalence in developing countries, which leads to negative health outcomes for both mothers and children. Maternal stress varies over the course of pregnancy and the stress occurring in early pregnancy is the most critical. However, few studies have focused on the impact of resilience to stress on mental health of pregnant women. Aims To explore the effect of resilience to stress on prenatal anxiety/depression in pregnant women. Methods 2813 participants were recruited from Shanghai Maternal-Child Pairs Cohort (Shanghai MCPC) in this study. The Life Event Scale for Pregnancy Women (LESPW) was used to assess stress at 12–16 weeks of pregnancy and at 32–36 weeks of pregnancy, respectively. Resilience was assessed by the revised Resilience Scale for Adults (RSA) at 12–16 weeks of pregnancy. The prenatal anxiety and depression were assessed at 32–26 weeks of pregnancy by Self-Rating Anxiety Scale (SAS) and the Center for Epidemiological Survey, Depression Scale (CES-D), respectively. Hierarchical linear regression analyses were conducted to explore the direct effect of stress and resilience on prenatal anxiety and depression. The indirect effects of mediation were analyzed by structural equation models, and the indirect effect of modification was examined by stratification analysis. Results There were 11.1% and 10.3% of pregnant women in Shanghai MCPC indicating anxiety and depressive mood, respectively. The stress caused by both subjective and objective events at 32–36 weeks of pregnancy is less than that at 12–16weeks of pregnancy. We found that resilience was a protective factor for maternal mental health and meanwhile it was both an effect modifier and a mediator to the association between stress and prenatal anxiety/depression, with the mediating effect ratio of 15.1% and 23.8%, respectively. Limitations The existence of recall bias, missing data, and restricted residential areas of the participants may limit the generalizability of the study. The measurements of resilience, prenatal anxiety, and depression were not designed specifically for pregnant women, so that they might have missed some important indications of mental issues related to pregnant women only. Conclusions Resilience could be a direct and indirect protective factor for prenatal anxiety and depression caused by pregnancy stress.

The Xiaoshuijing Syenogranite is the host of Mo mineralisation recently discovered in the Tengchong Terrane of southwestern China. Here, we present new zircon U-Pb ages and molybdenite Re-Os ages, whole-rock geochemical data, and zircon Hf isotopic data for the Xiaoshuijing Syenogranite hosting disseminated Mo mineralisation. The syenogranite’s mineralogical and geochemical characteristics indicate it is a high-K, alkaline, and peraluminous granite. LA-ICP-MS analysis of zircon yields an age of 65 ± 1 Ma (n = 14, MSWD = 2.1), and yields negative zircon eHf(t) values ranging from −18.23 to −12.92, interpreted to have a crustal source. The Re-Os Mo age of 64 ± 2 Ma (n = 4, MSWD = 2.2) for the mineralisation is the same as the syenogranite’s age, within error. The geochemistry also indicates that the syenogranite is related to a thickened crust resulting from the eastward subduction of the Neo-Tethyan oceanic lithosphere shortly before the collision of the Indian and Eurasian continental crusts.

Background: The association between antibiotic use during pregnancy and neonatal birth outcomes has received considerable attention. Most of the previous assessment of antibiotic exposure during pregnancy relied on questionnaires and clinical prescriptions, and very few studies examined pregnancy exposure to antibiotics using human biomonitoring data. Objective: To explore the association between the cumulative exposure of antibiotics during the whole pregnancy and neonatal birth measurements using biomonitoring data of antibiotics in meconium. Methods: Three hundred and sixty nine pregnant women within the Maternal Psychological and Environmental Assessments of Kids Cohort Study were randomly selected into this study. Eighteen common antibiotics of six categories (six β‑lactams, three tetracyclines, four sulfonamides, one phenicols, one lincosamides and three fluoroquinolones) were selected as the target antibiotics in meconium. The measurement was conducted by ultraperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry platform. Neonatal birth measurements were obtained from the medical records. Multiple linear regression models were used to examine the associations of antibiotic exposure with neonatal birth outcome (birth weight, birth length) and placental growth indicators (placental surface area, placental weight). Logistic regressions were used to evaluate associations with small for gestational age (SGA) and large for gestational age (LGA). Results: Twelve of the eighteen antibiotics were found in 62.1% of the meconium, with detection rates ranging from 0.3% to 43.9%. The three antibiotics with the highest detection rates were chlortetracycline (43.9%), penicillin (16.5%) and chloramphenicol (10.8%), respectively. The highest antibiotic concentration among detected antibiotics was penicillin (24,243.15 μg/kg). The concentration of penicillin was positively associated with the birth weight (β: 0.025; 95% CIs: 0.003–0.047). A significant positive association was also observed between the concentration of chlortetracycline and the placental surface area (β: 2.559; 95% CIs: 0.296–4.822). These associations were sex related and mainly observed in female newborns. Exposure to penicillin was also found to be associated with increased risk of LGA, which was consistent with changes in birth weight. Conclusions: Pregnancy exposure to certain antibiotics was associated with altered fetal growth and development, which may affect the normal growth trajectory of infants and children in later life. Keywords: Cumulative exposure of antibiotics, Meconium, Biomonitoring data, Birth outcomes, Placental growth indicators, LGA

Carbon capture has become an important technology to mitigate ever-increasing CO2 emissions worldwide, and alkali waste is a potential source of CO2 capture material. Slagging-gasification is a novel technology for treating municipal solid waste (MSW), and the gasification fly ash (GFA) is the only solid residue that is not reused at present due to its high heavy metal content. GFA contains high amounts of Ca(OH)2 and Ca(OH)Cl, making it protentional for CO2 capture. In this study, GFA and washed gasification fly ash (WGFA) were treated with CO2 for different treatment periods. Weight changes of samples were recorded to evaluate the efficiency of CO2 capture. To assess the properties of treated GFA, pH value, leached heavy metal concentration, mineral composition, and microscopic morphology were studied. The results revealed that GFA and WGFA could adsorb 18.8% and 23.7% CO2 of their weights, respectively. Carbonation could immobilize heavy metals including Pb, Zn, and Cu when a proper treatment period was applied. An excessive treatment period decreased the efficiency of heavy metal immobilization. Pre-washing is recommended as a pre-treatment method for GFA carbonation, which increased the efficiency to adsorb CO2, improved the pH of carbonated GFA, and enhanced the effect to immobilize heavy metals. Ministry of Education (MOE) This research is supported by the Ministry of Education, Singapore, under its Academic Research Fund Tier 1 grant (RG139/20).

Maternal emotional symptoms during pregnancy increase the risk of neurodevelopmental problems in offspring, and microbiota have been shown to be a potential mechanism underlying the link. However, the associations among maternal prenatal emotional symptoms, the meconium microbiota, and offspring neurodevelopment have yet to be fully elucidated. The aim of this prospective cohort study was to assess the relationship between maternal prenatal emotional symptoms and neurodevelopment of the child at 24 months of age, and to investigate the potential role of the neonatal meconium microbiota in the relationship.A total of 410 mother-child pairs (152 women in the Symptoms group vs. 258 women in the No-symptoms group) were recruited from the ongoing Shanghai Maternal-Child Pairs Cohort. This study included a subgroup of women who were assessed for maternal anxiety and depressive symptoms at 32-36 weeks of gestation. Neonatal meconium samples were collected after birth for 16 S sequencing. Children's neurodevelopment was measured using the Ages and Stages Questionnaire, Third Edition (ASQ-3) and Strengths and Difficulties Questionnaire (SDQ) at 24 months postnatally (n = 287).Compared with the No-symptoms group, children in the Symptoms group had a higher degree of hyperactivity and total difficulties at 24 months of age. Increases in alpha diversity, distinct overall composition, enriched relative abundance of Proteobacteria, and different predicted microbial functions were observed in the meconium of neonates exposed to maternal prenatal emotional symptoms. The neonatal gut microbiota alpha diversity and relative abundance of genera from the Proteobacteria phylum and Lactobacillus were negatively correlated with children's degree of prosocial behavior, tendency toward hyperactivity, and poor fine motor development. In addition, mediating effects of neonatal meconium microbial richness and the relative abundance of Lactobacillus were observed between maternal emotional symptoms and children's prosocial behavior.Maternal prenatal emotional symptoms are associated with alterations in the offspring meconium microbiota and children's neurodevelopment at 24 months of age, and the microbial richness indices and Lactobacillus may play a mediating role. Future research is needed to identify and understand the biological pathways and metabolisms linking the relationships among maternal emotional symptoms, meconium microbiota, and neurodevelopment of children.

Cadmium (Cd) pollution in mining areas is the most important challenge for soil environment management in China. Assessing the actual Cd pollution risk in various mining areas and identifying the core areas requiring supervision can provide a basis for government departments and industries to carry out detailed further investigations in key areas. In this study, we collated published data on metal mine circumjacent soil contaminated by Cd in China from 2002 to 2020 to conduct a comprehensive study on soil cadmium pollution and ecological and health risks in mining areas. The temporal and spatial variations of Cd concentrations and the pollution source were investigated. Results indicated that the Cd concentration in soil was strongly associated with the types of mining area. The Cd pollution in the circumjacent soil of lead-zinc and tungsten mines with high heavy metal pollution discharging coefficient was more serious than the soil around other mines. Identification of temporal and spatial variations for soil Cd in China indicated that the high Cd concentrations were found in the central, southern, and southwestern regions of China, and the distribution of mining activities in these regions are relatively concentrated. Meanwhile, a temporal turning point in the mean soil Cd concentration occurred in these regions in 2012, which indicated that the heavy metal control management policy implemented by the government was effective. The ecological risk of soil Cd pollution around mining areas was moderate to high. Health risk assessment showed that some regions adjacent mining areas had a high non-carcinogenic risk, notably, lead-zinc and tungsten mining areas were more serious. Supervision should focus on reducing ecological risks and protecting the safety of agricultural products rather than concentrating on health risks. The research results provide a reference for the priority management of contaminated soil in mining areas.

Conventional adsorbents for decontaminating arsenic exhibit low efficacy for the removal of arsenite (As(III)). This study aims to develop a robust As adsorbent from natural magnetite (M0) via a facile ball milling process, and evaluate their performance for decontaminating As(III) and As(V) in water and soil systems. The ball milling process decreased the particle size and crystallinity of M0, resulting in pronounced As removal by the ball-milled magnetite (Mm). Ball milling under air facilitated the formation of Fe-OH and Fe-COOH functional groups on Mm interface, contributing to effective elimination of As(III) and As(V) via hydrogen bonding and complexation mechanisms. Synergistic oxidation effects of hydroxyl and carboxyl groups, and reactive oxygen species (O2·-, and ·OH) on the transformation of As(III) to As(V) during the adsorption were proposed to explain the enhanced As(III) removal by Mm. A short-term soil incubation experiment indicated that the addition of Mm (10 wt%) induced a decrease in the concentration of exchangeable As by 30.25%, and facilitated the transformation of water-soluble As into residual fraction. Ball milling thus is considered as an eco-friendly (chemical-free) and inexpensive (scalable, one-stage process) method for upgrading the performance of natural magnetite towards remediating As, particularly for tackling the highly mobile As(III). Refereed/Peer-reviewed

Molecular mechanisms underlying wing evolution and development have been a point of scientific inquiry for decades. Phloem-feeding aphids are one of the most devastating global insect pests, where dispersal of winged morphs lead to annual movements, migrations, and range expansions. Aphids show a polyphenic wing dimorphism trait, and offer a model to study the role of environment in determining morphological plasticity of a single genotype. Despite recent progresses in the genetic understanding of wing polyphenism, the influence of environmental cues remains unclear. To investigate the involvement of miRNAs in wing development, we sequenced small RNA libraries of the English grain aphid, Sitobion avenae (F.), across six different developmental stages. As a result, we identified 113 conserved and 193 S. avenae-specific miRNAs. Gene Ontology and KEGG pathway analyses of putative target mRNAs for the six differentially expressed miRNAs are enriched for wing development processes. Dietary uptake of miR-263a, miR-316, and miR-184a agomirs and antagomirs led to significantly higher mortality (>70%) and a lower proportion of winged morphs (

The applications of biogenic Mn oxides (BMOs) formed by Mn-oxidizing fungus in decontaminating heavy metals have attracted increasing attention. In this study, an efficient Mn-oxidizing fungus was isolated from soil and identified as Cladosporium halotolerans strain XM01. The Mn(II) adsorption and oxidation activities of this strain were investigated, showing significantly high removal and oxidation rates of soluble Mn(II) of 99.9% and 88.2%, respectively. Dynamic analysis of the Mn(II) removal process demonstrated the oxidation process of Mn(II) to Mn(III) was the rate-limiting step in the Mn(II) metabolic process. The XRD and SAED characterization showed that more layers were orderly accumulated along the c-axis with the formation of fungal BMOs, which might lead to the decrease in its specific surface area. The adsorption of Cd(II) by the formed BMOs was investigated and compared with two typical abiotic Mn oxides, indicating that the adsorption capacity decreased with the following order: immature BMO, mature BMO, δ-MnO2, acid birnessite, while the fixation capacity decreased in the order of acid birnessite, mature BMO, δ-MnO2, immature BMO. The inverse correlation between the capacity of Cd(II) adsorption and fixation of immature and mature BMOs was probably attributed to the increase in the layer stacking of BMOs. This result indicates an interesting phenomenon of high reservation of Cd(II) resulting from sequential transformation from strong adsorption to strong fixation with the formation of BMOs. This study offers considerable insights into fungal Mn oxidation mechanisms and provides theoretical guidance for fungal BMOs in heavy metals bioremediation.

Human exposure to lead (Pb) is a growing global public health concern. Elevated blood lead is thought to cause the mental retardation of >0.6 million children globally each year, and has recently been attributed to ~18% of all-cause mortality in the US. Due to the severe health risk, the international community, led by the United Nations Environment Programme (UNEP) and the World Health Organization (WHO), is actively supporting the global phase-out of lead-based paint by 2020. However, there are many significant hurdles on the way to achieving this goal. In light of the importance of the lead-based paint issue, and the urgency of achieving the 2020 phase-out goal, this review provides critical insights from the existing scientific literature on lead-based paint, and offers a comprehensive perspective on the overall issue. The global production and international trade of lead-based paints across Asia, Africa, Latin America, and Europe are critically discussed – revealing that lead-based paints are still widely used in many low and middle-income developing countries, and that the production and trade of lead-based paint is still wide-spread globally. In India, as well as many south-east Asian, African, Latin American and European countries, lead concentrations in paints often exceed 10,000 mg/kg. This will certainly pose a serious global threat to public health from surfaces painted with these products for many decades to come. The sources and pathways of exposure are further described to shed light on the associated health risk and socioeconomic costs. Finally, the review offers an overview of the potential intervention and abatement strategies for lead-based paints. In particular, it was found that there is a general lack of consensus on the definition of lead based paint; and, strengthening regulatory oversight, public awareness, and industry acceptance are vital in combating the global issue of lead based paint. Keywords: Lead-based paint, Lead exposure, Blood lead level, Soil contamination

The reactions of ethyl and butyl xanthates with Pb2+, Cd2+ and Zn2+ were investigated to determine the solubility products of metal xanthates using UV/Visible spectroscopic method and software WinSGW and MEDUSA. The effects of solution pH, chemical reactions and ionic strength on the determination of solubility products of metal xanthates were investigated. For a given metal, the solubility product of metal xanthate was found to decrease with the increase of the carbon chain length of the alkyl group. The determined solubility products of metal xanthates were in the order of ZnX2 > CdX2 > PbX2 and M(EX)2 > M(BX)2. The accurate Ksp constant allowed more accurate calculation of the minimal concentration of xanthate ions for treating heavy metal-containing waste water to selectively separate metal ions, avoiding the potential presence of toxic xanthates in the treated water.

Previous studies have linked prenatal polybrominated diphenyl ether (PBDE) exposure to adverse birth outcomes. Reductions in placental size might mediate those adverse effects. We examined whether umbilical cord blood PBDEs concentrations were associated with placental size at birth. A total of 121 mother-newborn pairs residing in Wenzhou were enrolled in this study. Concentrations of 19 PBDEs in umbilical cord blood were determined by gas chromatography tandem mass spectrometry (GC-MS). Placental length, breadth and surface area was measured by medical professionals after delivery. BDE-206, 207, 208, 209 and the total of 19 PBDE congeners concentrations were negatively associated with placental length, breadth and surface area. Our results suggest that prenatal PBDEs exposure may suppress placental growth, resulting in reduced placental size.

The Early Cretaceous granitoids that are widespread in the Tengchong terrane of Southwest China play a critical role in understanding the tectonic framework associated with the Tethyan oceans. In this study, we present a detailed description of zircon U–Pb ages, whole-rock geochemistry and Hf isotopes for the Laoxiangkeng pluton in the eastern Tengchong terrane and elucidate their petrogenesis and geodynamic implications. Zircon U–Pb dating of the Laoxiangkeng pluton yields ages of 114 ± 1 Ma and 115 ± 1 Ma, which imply an Early Cretaceous magmatic event. The Laoxiangkeng pluton enriched in Si and Na, is calc-alkaline and metaluminous, and has the characteristics of highly fractionated I-type granites. Zircons from the pluton have calculated e Hf ( t ) values of −12.7 to −3.7 and two-stage model ages of 1327–1974 Ma, respectively, indicating a mixed source of partial melting of Paleo-Neoproterozoic crust-derived compositions with some inputs of mantle-derived magmas. By integrating all available data for the regional tectonic evolution of the eastern Tethys tectonic domain, we conclude that the Early Cretaceous magmatism in the Tengchong terrane was produced by the northeastward subduction of the Meso-Tethyan Bangong–Nujiang Ocean.

3D shapes and 2D images usually contain complementary information for each other, and thus joint analysis of both of them will benefit some problems existing in different domains. Leveraging the connection between 2D images and 3D shapes, it's potential to mine lacking information of one modal from the other. Stemming from this insight, we design and implement a CNN architecture to jointly analyze shapes and images even with few training data guidance. The core of our architecture is a domain adaptation algorithm, which builds up the connection between underlying feature spaces of images and shapes, then aligns and correlates the intrinsic structures therein. The proposed method facilitates the recognition and retrieval tasks. Experiments on the shape recognition tasks show that our approach has superior performance under the difficult setting: zero-shot learning and few-shot learning. We also evaluate our method on the retrieval tasks, and demonstrate the effectiveness of the proposed method.

Gestational diabetes mellitus (GDM) has become a new global epidemic with a rapidly increasing prevalence. Previous studies have suggested that air pollution is associated with GDM risk, but the results are inconsistent, and mechanistic studies are limited. Based on a hospital-based cohort, a total of 6374 participants were included in this study. Individual daily PM2.5 exposure at a 1-km resolution was predicted using a full-spatiotemporal-coverage model. The results of multiple linear regression showed that glycated hemoglobin (HbA1c) was significantly associated with PM2.5 both in the 1-month preconception and in the first trimester of pregnancy. Additionally, HbA1c decreased 0.437% (95% CI: − 0.629, − 0.244) as the serum 25-hydroxyvitamin D (25(OH)D) increased by one interquartile range (IQR) (9.2 ng/ml). An IQR increase in PM2.5 exposure was also negatively associated with serum 25(OH)D (estimated change% and 95% CI: − 7.249 (− 9.054, − 5.408) in the 1-month preconception and − 13.069 (− 15.111, − 10.979) in the first trimester of pregnancy). Mediation analysis showed that serum 25(OH)D status mediated the association between HbA1c and PM2.5 exposure both in the preconception and in the first trimester (mediated percent: 2.00% and 4.05% (Sobel p ＜ 0.001), respectively). The result suggested a vicious cycle among PM2.5 exposure, lower serum VD status and a higher HbA1c. More studies are warranted since the protective effect of 25(OH)D against glucose disorders associated with air pollution in this study was limited.

Enterovirus A71 (EV-A71) is an important emerging virus posing a threat to children under five years old. Circular RNAs (circRNAs), a novel class of endogenous RNAs, have been recognized to play important roles in the onset and development of viral diseases. However, it has not been determined which specific circRNAs are involved in the pathological mechanisms of EV-A71 infection. In this study, RNA-sequencing (RNA-seq) was conducted to characterize differentially expressed circRNAs during the process of EV-A71 infection. Overall, 8726, 10405 and 4710 circRNAs were detected in the control, EV-A71-12 h and EV-A71-24 h groups, respectively, of which 1851 and 951 circRNAs were differentially expressed in the EV-A71-12 h and EV-A71-24 h groups versus the control group. The overlapping circRNAs in the EV-A71-infected groups were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, which further demonstrated that the host genes of these circRNAs were principally implicated in activities associated with the progression of viral infection, such as immune system process, Wnt signaling pathway, etc. Additionally, qRT-PCR detection showed that six selected circRNAs were identical to the sequencing data. To excavate the key circRNAs in EV-A71 infection, we comprehensively evaluated and integrated the relationship of circRNA/miRNA/mRNA, and eventually screened 2 key circRNA regulatory axes, namely hsa_circ_0017115/hsa-miR-150-5p/EGR1 and hsa_circ_0005060/hsa-miR-4685-5p/MMP2. In summary, our findings not only provide the first comprehensive expression and functional profile of circRNAs in response to EV-A71 infection, but also offer a novel direction to elucidate the molecular mechanism underlying viral pathogenesis and the cellular immune response in host-EV-A71 interactions.

Triptolide (TP), a primary bioactive ingredient isolated from the traditional Chinese herbal medicine Tripterygium wilfordii Hook. F. (TWHF), has attracted great interest for its therapeutic biological activities in inflammation and autoimmune disease. However, its clinical use is limited by severe testicular toxicity, and the underlying mechanism has not been elucidated. Our preliminary evidence demonstrated that TP disrupted glucose metabolism and caused testicular toxicity. During spermatogenesis, Sertoli cells (SCs) provide lactate as an energy source to germ cells by glycolysis. The transcription factors GATA-binding protein 4 (GATA4) and specificity protein 1 (Sp1) can regulate glycolysis. Based on this evidence, we speculate that TP causes abnormal glycolysis in SCs by influencing the expression of the transcription factors GATA4 and Sp1. The mechanism of TP-induced testicular toxicity was investigated in vitro and in vivo. The data indicated that TP decreased glucose consumption, lactate production, and the mRNA levels of glycolysis-related transporters and enzymes. TP also downregulated the protein expression of the transcription factors GATA4 and Sp1, as well as the glycolytic enzyme phosphofructokinase platelet (PFKP). Phosphorylated GATA4 and nuclear GATA4 protein levels were reduced in a dose- and time-dependent manner after TP incubation. Similar effects were observed in shGata4-treated TM4 cells and BALB/c mice administered 0.4 mg/kg TP for 28 days, and glycolysis was also inhibited. Gata4 knockdown downregulated Sp1 and PFKP expression. Furthermore, the Sp1 inhibitor plicamycin inhibited PFKP protein levels in TM4 cells. In conclusion, TP inhibited GATA4-mediated glycolysis by suppressing Sp1-dependent PFKP expression in SCs and caused testicular toxicity.

Abundant geothermal springs occur along the Moxi fault located in western Sichuan Province (the eastern edge of the Qinghai-Tibet plateau), highlighted by geothermal water outflow with an unusually high temperature of 218 °C at 21.5 MPa from a 2010-m borehole in Laoyulin, Kangding. Earthquake activity occurs relatively more frequently in the region and is considered to be related to the strong hydrothermal activity. Geothermal waters hosted by a deep fault may provide evidence regarding the deep underground; their aqueous chemistry and isotopic information can indicate the mechanism of thermal springs. Cyclical variations of geothermal water outflows are thought to work under the effect of solid earth tides and can contribute to understanding conditions and processes in underground geo-environments. This paper studies the origin and variations of the geothermal spring group controlled by the Moxi fault and discusses conditions in the deep ground. Flow variation monitoring of a series of parameters was performed to study the geothermal responses to solid tides. Geothermal reservoir temperatures are evaluated with Na-K-Mg data. The abundant sulfite content, dissolved oxygen (DO) and oxidation-reduction potential (ORP) data are discussed to study the oxidation-reduction states. Strontium isotopes are used to trace the water source. The results demonstrate that geothermal water could flow quickly through the Moxi fault the depth of the geothermal reservoir influences the thermal reservoir temperature, where supercritical hot water is mixed with circulating groundwater and can reach 380 °C. To the southward along the fault, the circulation of geothermal waters becomes shallower, and the waters may have reacted with metamorphic rock to some extent. Our results provide a conceptual deep heat source model for geothermal flow and the reservoir characteristics of the Moxi fault and indicate that the faulting may well connect the deep heat source to shallower depths. The approach of hot spring variation research also has potential benefits for earthquake monitoring and prediction.

ZSM-5, as a hydrophobic zeolite, has a good adsorption capacity\ud for methyl tert-butyl ether (MTBE) in batch adsorption studies. This\ud study explores the applicability of ZSM-5 as a reactive material in\ud permeable reactive barriers (PRBs) to decontaminate the MTBE-containing\ud groundwater. A series of laboratory scale fixed-bed column tests were\ud carried out to determine the breakthrough curves and evaluate the\ud adsorption performance of ZSM-5 towards MTBE under different operational\ud conditions, including bed length, flow rate, initial MTBE concentration\ud and ZSM-5 dosage, and regeneration tests were carried out at 80, 150 and\ud 300°C for 24 h. Dose-Response model was found to best describe the\ud breakthrough curves. MTBE was effectively removed by the fixed-bed column\ud packed with a ZSM-5/sand mixture with an adsorption capacity of 31.85\ud mg∙g-1 at 6 cm bed length, 1 mL∙min-1 flow rate, 300 mg∙L-1 initial MTBE\ud concentration and 5% ZSM-5 dosage. The maximum adsorption capacity\ud increased with the increase of bed length and the decrease of flow rate\ud and MTBE concentration. The estimated kinetic parameters can be used to\ud predict the dynamic behaviour of column systems. In addition,\ud regeneration study shows that the adsorption capacity of ZSM-5 remains\ud satisfactory (>85%) after up to four regeneration cycles.

Knowledge of the origins of polycyclic aromatic hydrocarbon (PAH) in vegetables is essential to reduce human health risks induced by dietary exposure. The current study developed a vegetation-advanced multimedia model, SESAMe-Veg, to identify the major uptake pathway of 15 priority PAHs in vegetables and assess the PAHs in edible parts of cabbages and carrots in Jinzhong City, Shanxi Province, China. The model was well evaluated against site- and plant-specific measurements. Edible parts exhibited lower PAH concentrations than the other parts for both vegetables. The estimated concentrations of ΣPAH15 were 79 ng/g in cabbage shoots and 83 ng/g in carrot roots. Higher concentrations were estimated in shoots of the leafy vegetable than in roots of the root vegetable for most PAHs. Although air-shoot is the major transport pathway, 98% was deposition of particles, which was attached outside and could be removed relatively easily by washing. Soils might be the origin of PAHs inside vegetables, especially for lighter PAHs. PYR was more likely to be stored in roots than other congeners. The translocation of PAHs inside vegetables was negligible. Adulthood dietary exposure to local vegetables probably caused a high health risk; however, contributions from consuming cabbages and especially carrots were low. Females generally exhibited slightly higher risks than males of exposure to PAHs in local vegetables. Considering the dominant role of particle deposition, carefully vegetable washing before ingestion could reduce this risk. This study has provided a functional tool to evaluate vegetable contamination by PAHs. Capsule A vegetation-advanced multimedia model of PAHs in different parts of vegetables and other environmental media was developed to evaluate the potential health risk to local populations of different sexes and ages via vegetable ingestion.