1. Coexpressed Auxiliary Subunits Exhibit Distinct Modulatory Profiles on AMPA Receptor Function
- Author
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Alexandru Rusu, Ling Zhang, Jakob von Engelhardt, Paul Farrow, Anton Schulmann, Konstantin Khodosevich, Eric Jacobi, Rolf Sprengel, and Hannah Monyer
- Subjects
Cell type ,physiology [Excitatory Postsynaptic Potentials] ,Patch-Clamp Techniques ,Neuroscience(all) ,Cell ,Nerve Tissue Proteins ,AMPA receptor ,Gating ,genetics [Neuronal Plasticity] ,Biology ,Synaptic Transmission ,Gene Knockout Techniques ,Mice ,methods [Patch-Clamp Techniques] ,medicine ,physiology [Neuronal Plasticity] ,Animals ,Humans ,ddc:610 ,Receptors, AMPA ,Neurons ,metabolism [Nerve Tissue Proteins] ,CKAMP44 protein, mouse ,Neuronal Plasticity ,General Neuroscience ,Dentate gyrus ,musculoskeletal, neural, and ocular physiology ,metabolism [Receptors, AMPA] ,metabolism [Calcium Channels] ,Excitatory Postsynaptic Potentials ,Membrane Proteins ,Long-term potentiation ,metabolism [Dentate Gyrus] ,Receptor Desensitization ,physiology [Neurons] ,Cell biology ,Electrophysiology ,medicine.anatomical_structure ,nervous system ,metabolism [Neurons] ,physiology [Synaptic Transmission] ,Dentate Gyrus ,Synapses ,genetics [Receptors, AMPA] ,Calcium Channels ,physiology [Synapses] ,metabolism [Membrane Proteins] ,TARP gamma-8 protein, mouse - Abstract
Summary Gating properties and surface trafficking of AMPA receptors (AMPARs) are modulated by auxiliary subunits. Here we studied the function of coexpressed auxiliary subunits belonging to two different classes. We focused on TARP γ-8 and CKAMP44 in dentate gyrus (DG) granule cells, since both subunits are highly expressed in this cell type. TARP γ-8 and CKAMP44 decrease the rate of deactivation but have an opposing influence on receptor desensitization, which accounts for their differential modulation of synaptic short-term plasticity. Furthermore, long-term plasticity (LTP) requires TARP γ-8 but not CKAMP44. The coexpression of both auxiliary subunits is necessary for the efficient targeting of AMPARs to the cell surface of DG granule cells. Finally, electrophysiological and biochemical evidence support the notion that CKAMP44 and TARP γ-8 can be contained in the same AMPAR complex. Video Abstract
- Published
- 2014
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