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Background and Aims To investigate the superiority of individualized dietary advice based on dietary assessment for patients with type 2 diabetes. Methods and Results A total of 136 Japanese adults with type 2 diabetes were randomized into either individualized or conventional dietary advice groups after dietary assessment using a self-administered brief-type diet history questionnaire. All participants received three 30-minute face-to-face dietary advice sessions by dietitians at 1, 3, 5 months from study entry. The individualized group received dietary advice based on individual dietary intakes. The conventional group received dietary advice using generalized pamphlets. The primary outcome was the change in HbA1c over 6 months, and secondary outcomes were changes in weight, serum triglyceride, high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), and dietary intakes. In total, 126 participants were included in the analysis. After adjustment for age, sex, and baseline measurements, HbA1c significantly decreased larger in the individualized group [−1.1%, 95% CI: −1.3 to −0.8)] than the conventional group [−0.7% (95% CI: −1.0 to −0.4)] (P=0.0495). Weight, serum triglyceride, and LDL-C significantly lowered, and HDL-C significantly increased only in the individualized group, without a significant difference to the conventional group. In dietary changes, the individualized group decreased intakes of energy, confectioneries, meats, oil and fats, and sugar-sweetened beverages. The conventional group decreased alcohol intake, and increased total fat and saturated fatty acid intakes. Conclusions Individualized dietary advice among patients with type 2 diabetes was superior to conventional dietary advice in lowering HbA1c. Trial Registration UMIN000037268 ( https://www.umin.ac.jp/ctr/index.htm ) in July 4, 2019.

Palm fatty acid distillate (PFAD), a by-product of refining process of crude palm oil can be used as a potential feedstock for biodiesel production. However, the application of palm oil-based biodiesel is often hinder by its poor cold flow properties (CFP). Biodiesel fuel with poor CFP may crystallize and result in clogging of fuel lines, filters and injectors that cause engine operability problems. For that, a vacuum distillation method was designed and its feasibility and efficiency in improving the CFP was examined. A total of 13.60wt% of total saturated fatty acid methyl esters were successfully removed from the PFAD biodiesel, resulting in the improvement of the cloud point (CP), cold filter plugging point (CFPP) and pour point (PP) of PFAD biodiesel from 20 °C, 19 °C, and 15 °C to 13 °C, 11 °C, and 9 °C, respectively. It is remarkable that the improved CFPP satisfied the requirements for grade C summer biodiesel for temperate climates in EN 14212 standard. Additionally, Sarin (UFAME) empirical correlation was evaluated and it was found to have a good prediction of CFP for PFAD biodiesel, with lower than 2 °C deviation.

The family of Brassicaceae accounts for one of the earliest cataloged annual oilseed referred to as Mustard. Brassica nigra and Brassica rapa var. toria are two of the commercially important varieties of mustard seed which are endowed with biomedical properties. The present study was designed for the comparative scrutiny of quality and physicochemical parameters of both oilseed mustard varieties. Oil content, near infrared reflectance analysis, moisture content, color, pungency, free fatty acid value, acid value, presence of argemone test and gas chromatography were studied. B. nigra and B. rapa toria showed oil content corresponding to 37.68% and 43.06% respectively. Less moisture content was observed in B. rapa toria (5.33 %) in comparison to B. nigra. Higher color units were investigated in B. nigra in comparison to B. rapa toria. B. rapa toria showed lesser pungency with 0.034 % allyl isothiocynate content. B. nigra illustrated free fatty acid (FFA) content of 0.79% and high acid value (1.58%). While B.rapa toria showed less FFA and acid value. Argemone oil contamination was not seen in oil of both varieties. B. nigra showed 6.45%, 69.25%, 24.29% of saturated, monounsaturated and polyunsaturated fatty acids respectively. B. rapa toria demonstrated valuable gas chromatographic analysis with lesser saturated fatty acid and higher monounsaturated fatty acid. In comparison to B. nigra, higher oil content, lesser moisture, low FFA value, less acid value and favorable fatty acid composition analysis has been shown by B. rapa toria there upon, indicating superior quality of B. rapa toria oilseed.

Aims There is still controversy on optimal percentage of macronutrient intakes especially carbohydrate on prevention of type 2 diabetes (T2D). The aim of the current study was to evaluate the iso-energetic substitution of dietary macronutrients in relation to incidence of T2D. Methods This prospective study was conducted on 6547 subjects who were followed for 3.0 ± 1.6 years. Dietary intakes were evaluated using a valid and reliable semi-quantitative food frequency questionnaire. Biochemical variables and anthropometrics were evaluated at baseline and follow-up examinations. Multivariate Cox proportional hazard regression models were used to estimate the hazards ratio (HR) of T2D. Results A one unit higher proportion of carbohydrate from whole grain at the expense of carbohydrate from non-whole grain (HR = 0.82, 95% CI = 0.71–0.95), protein (HR = 0.75, 95% CI = 0.60–0.94), total fat (HR = 0.79, 95% CI = 0.67–0.93), saturated fatty acid (HR = 0.79, 95% CI = 0.65–0.96), poly-unsaturated fatty acid (HR = 0.81, 95% CI = 0.68–0.96) and mono-unsaturated fatty acid (HR = 0.75, 95% CI = 0.60–0.94) was associated with decreased risk of T2D. The substitution of protein and fats was not associated with diabetes. Risk of T2D increased from quartiles 1–4 for trans-fatty acid (HR: 1.00, 1.27, 0.95, 1.97, Ptrend = 0.01). Conclusions A higher proportion of carbohydrate from whole grain in replacement of refined grain, and other macronutrients was associated with decreased risk of T2D. Also our study suggests a positive association between trans-fatty acid intake and risk of T2D.

The monounsaturated fatty acid (MUFA) oleic acid (OA) has previously been shown to reverse saturated fatty acid palmitic acid (PA)-induced hepatic insulin resistance (IR). However, its underlying molecular mechanism is unclear. In addition, previous studies have also shown that the ω-3 polyunsaturated fatty acid (PUFA) eicosapentaenoic acid (EPA) reverses PA-induced muscle IR, but whether EPA plays the same role in hepatic IR and its possible mechanism involved need to be further clarified. Here, we confirmed that EPA reversed PA-induced IR in HepG2 cells and compared the proteomic changes after treatment with different free fatty acids (FFAs). A total of 234 proteins were determined to be differentially expressed after MUFA treatment. Their functions were mainly related to responses to stress and endogenous stimuli, lipid metabolic process, and protein binding. For PUFA treatment, the PA-induced expression changes of 1326 proteins could be reversed by EPA treatment, 415 of which were mitochondrial proteins, with most of the functional proteins involved in oxidative phosphorylation (OXPHOS) and tricarboxylic acid (TCA) cycle. Mechanistic studies revealed that the protein encoded by JUN and reactive oxygen species (ROS) play a role in OA- and EPA-reversed PA-induced IR, respectively. EPA or OA alleviated PA-induced abnormal adenosine triphosphate (ATP) production, ROS generation, and calcium (Ca2+) content. Importantly, H2O2-activated production of ROS increased the protein expression of JUN, further resulting in IR in HepG2 cells. Taken together, we demonstrate that ROS/JUN is a common response pathway employed by HepG2 cells toward FFA-regulated IR.

Background: Chronic consumption of dairy products with a saturated fatty acid (SFA)-reduced, monounsaturated fatty acid (MUFA)-enriched content was shown to impact favourably on brachial artery flow-mediated dilatation (FMD). However, their acute effect on postprandial cardiometabolic risk biomarkers requires investigation.\ud \ud Objective: The effects of sequential high-fat mixed meals rich in fatty acid (FA)-modified or conventional (control) dairy products on postprandial FMD (primary outcome) and systemic cardiometabolic biomarkers in adults with moderate cardiovascular risk (≥50% above population mean) were compared. \ud \ud Methods: In a randomized cross-over trial, fifty-two participants (mean ± SEM age 53 ± 2 y; BMI 25.9 ± 0.5 kg/m2) consumed high-dairy fat breakfast (0 min; ~50 g total fat: Modified: 25 g SFAs, 20 g MUFAs; Control: 32 g SFAs, 12 g MUFAs) and lunch (330 min; ~30 g total fat; Modified: 15 g SFAs, 12 g MUFAs; Control: 19 g SFAs, 7 g MUFAs). Blood samples were obtained before and until 480 min after breakfast, with FMD assessed at 0, 180, 300 and 420 min. Data were analysed by linear mixed models.\ud Results: Postprandial changes in cardiometabolic biomarkers were comparable between the different dairy meals, with the exception of a tendency for a 4% higher area under the curve (AUC) for the %FMD response following the modified dairy fat meals (P = 0.075). Plasma total lipid FA analysis revealed that incremental AUC responses were 53% lower for total SFAs, 214% and 258% higher for total cis-MUFAs (predominantly cis-9 18:1), and trans-18:1 respectively following the modified, relative to control dairy meals (all P < 0.0001). \ud \ud Conclusions: In adults at moderate cardiovascular risk, acute consumption of sequential high-fat meals containing FA-modified dairy products had little impact on postprandial endothelial function or systemic cardiometabolic biomarkers, but a differential effect on the plasma total lipid FA profile, relative to conventional dairy fat meals.

Macronutrients - carbohydrates, fats, and proteins - supply the nutrients required for optimal functioning. Inadequate intake compromises both physical and brain health. We synthesized research on macronutrients from whole meals on cognitive function in healthy adults and identified underlying mechanisms. Intake of simple carbohydrates ('sugars') is consistently associated with decreased global cognition whereas consumption of complex carbohydrates correlates with successful brain aging and improved memory both in the short- and long-term. Saturated fatty acid intake correlates with decreased memory and learning scores whereas omega-3 intake correlates positively with memory scores. Protein intake boosts executive function and working memory when task-demands are high. Individual differences affecting the macronutrient-cognition relationship are age, physical activity, and glucose metabolism. Neural correlates reflect findings on cognitive functions: cortical thickness and cerebral amyloid burden correlate with sugar intake, inflammatory status and cerebral glucose metabolism correlate with fatty acid intake. Key mechanisms by which dietary macronutrients affect the brain and cognition include glucose and insulin metabolism, neurotransmitter actions, and cerebral oxidation and inflammation. In conclusion, macronutrient intake affects cognitive function both acutely and in the long-term, involving peripheral and central mechanisms. A healthy diet supports brain integrity and functionality, whereas inadequate nutrition compromises it. Studying diet can be key to nutritional recommendations, thereby improving the landscape of mental health and healthy brain aging.

A four-component skeletal mechanism consisting of 1,4-hexadiene, methyl decanoate, methyl trans-3-hexenoate, n-hexadecane was developed to represent biodiesel fuel from various sources. The methyl decanoate and n-hexadecane were selected to represent saturated fatty acid methyl esters, and trans-3-hexenoate and 1,4-hexadiene were applied to adjust degree of unsaturation. A skeletal mechanism for this four-component biodiesel surrogate fuel with PAH was first formulated based on decoupling methodology, which contained a detailed C1 mechanism, a reduced C2–C3 mechanism, PAH mechanism and sub-mechanisms of four surrogate fuels, including 314 reactions and 98 species. After that, the skeletal mechanism was widely verified against various fundamental combustion experiments for each pure component and their mixtures. Furthermore, the experimental results of biodiesel soot volume fractions from biodiesel spray combustion in a constant-volume combustion vessel were used to verify the accuracy of the mechanism, and the skeletal mechanism was also coupled into the CFD-software to simulate the combustion characteristics of a diesel engine. Results showed that the calculated results agreed well with the experimental data including ignition delay times (IDTs), primary species concentrations, laminar flame speed, soot prediction and in-cylinder pressure of the engine. Overall, the developed compact skeletal mechanism was suitable for the combustion simulation of biodiesel fuel.

Background: The saturated fatty acid stearic acid (C18:0) lowers HDL cholesterol compared with palmitic acid (C16:0). However, the ability of HDL particles to promote cholesterol efflux from macrophages (cholesterol efflux capacity; CEC) may better predict coronary heart disease (CHD) risk than HDL cholesterol concentrations.Objective: We examined effects of exchanging dietary palmitic acid for stearic acid on ATP-binding cassette transporter A1 (ABCA1)-mediated CEC, and other conventional and emerging cardiometabolic risk makers.Design: In a double-blind, randomized, crossover study with two 4-week isocaloric intervention periods, 34 healthy men and postmenopausal women (61.5 +/- 5.7 years, BMI: 25.4 +/- 2.5 kg/m(2)) followed diets rich in palmitic acids or stearic acids. Difference in intakes was 6% of daily energy. ABCA1-mediated CEC was measured from J774 macrophages to apolipoprotein (apo)B-depleted serum.Results: Compared with the palmitic-acid diet, the stearic-acid diet lowered serum LDL cholesterol (-0.14 mmol/L; p = 0.010), HDL cholesterol (-0.09 mmol/L; p =

Microalgae accumulate lipid triacylglycerol (TAG), a promising feedstock for production of natural edible oils and biofuels. To make products derived from microalgal TAG economically viable, increasing TAG content and productivity are of high importance. To increase TAG content, two endogenous key enzymes of TAG biosynthesis: plastidial lysophosphatidic acid acyltransferase (NeoLPAAT1) and endoplasmic reticulum-located diacylglycerol acyltransferase 2 (NeoDGAT2) were co-overexpressed in oleaginous microalga Neochloris oleoabundans. The neutral lipid content in NeoLPAAT1-NeoDGAT2 co-overexpressing transformant detected by Nile red staining increased 2-fold without compromising cell growth. The transcriptional levels of NeoLPAAT1 and NeoDGAT2 levels were 1.9-fold higher in the transformant than wild type. Considerably higher lipid accumulation was found in the transformant than wild type: total lipid content (73.72 ± 4.17 % DCW) increased 1.6-fold, TAG content (50.63 ± 6.15 % DCW) increased 2.1-fold, total lipid productivity (16.84 ± 0.66 mg/L/day) increased 1.9-fold, and TAG productivity (11.68 ± 0.90 mg/L/day) increased 2.1-fold. Fatty acid composition was slightly altered in the transformant compared to wild type; saturated fatty acid C16:0 increased to 26% from 20%, whereas C18:0 was reduced to 7% from 14%. Long-term stability of NeoLPAAT1-NeoDGAT2 co-overexpression was observed in the transformant continuously maintained on solid medium in a period of 4 years. The results suggested that targeted engineering of genes in pathway located at different organelles should be possible in microalgal lipid metabolism.

Sugar-smoking contributes to improving flavor attributes of meat products. However, there is rather limited information concerning the relationship between sugar-smoking process parameters and volatile compound (VC) fingerprinting as well as related quality attributes of sugar-smoked chicken. In this work, the changes in VC across the whole sugar-smoking process were determined and analyzed and physicochemical properties, free fatty acid, thiobarbituric acid reactive substances values, and E-nose were also performed to characterize the quality properties of sugar-smoked chicken breast (CB) and chicken skin (CS). Results suggested that a higher amount (P

In this study, iron sources and their optimum concentrations for high-quality biodiesel production were determined on Auxenochlorella protothecoides microalgae. Three iron compounds—ferrous sulfate, ferric ethylenediaminetetraacetic acid (EDTA), and ferric chloride—were assessed regarding concentration, specific growth rate, lipid, and lipid quality in relation to fatty acid methyl ester (FAME) composition of microalgal biomass. Auxenochlorella protothecoides demonstrated high resistivity to high concentrations of iron species, still showing an ability to grow in the presence of iron concentrations as high as 21.60 mM. The highest saturated fatty acid (SFA), at 78.5% of FAME, was observed at 1.15 mM ferric chloride, and the highest biomass generation was observed at 1.08 mM ferrous sulfate. In addition, biodiesel and diesel fuel qualities were satisfactory with microalgae cultivated at 0.2 and 14.4 mM ferrous sulfate, 7.19 mM ferric EDTA, and 0.07–21.58 mM ferric chloride concentrations. The former may be more practical, since the use of this iron compound gives the desired result in a broader range. Overall, it can be inferred that high-quality lipid and biodiesel production can be obtained by changing the concentrations and sources of iron compounds.

Spexin (SPX) is a novel satiety factor that putatively binds the galanin receptors R2 and R3 (GalR2/R3). SPX reduces body weight, and circulating SPX is decreased in obesity. It is unknown how SPX and its receptors are regulated in the hypothalamus, critical for energy homeostasis. We therefore examined the regulation of hypothalamic Spx, GalR2 and GalR3 gene expression in mouse primary and immortalized hypothalamic neurons. We report that Spx, GalR2 and GalR3 mRNA levels were regulated by acute treatments of palmitate, a dietary saturated fatty acid, as well as the nitric oxide (NO) donor sodium nitroprusside (SNP), but through a pathway independent of cyclic GMP and protein kinase G. Additionally, the palmitate- and NO-mediated induction of Spx and galanin receptors was blocked with the PKC inhibitor k252c. Furthermore, palmitate induced mRNA levels of endoplasmic reticulum (ER) stress markers, including Chop, Grp78 and Bax/Bcl2, as well as C/ebp-β, whereas SNP induced Bax/Bcl2 and C/ebp-β. Transcriptional changes in Spx, GalR2, GalR3, C/ebp-β and ER stress marker mRNAs were blocked by pre-treatment with at least one of the chemical chaperones PBA or TUDCA. We also describe the presence of OCT-1 and C/EBP-β response elements in the 5' regulatory region of Spx and demonstrate that SNP increases binding of C/EBP-β to this region, but not Oct-1 mRNA nor OCT-1 binding. Our findings suggest an acute modulation of anorexigenic SPX signaling by palmitate and NO. Furthermore, ER stress and C/EBP-β appear to mediate the changes in Spx, GalR2 and GalR3 in hypothalamic neurons.

Obesity is currently a worldwide phenomenon. The consumption of calorie-rich foods is responsible for most obesity cases, but not all humans exposed to high-calorie diets develop obesity. According to recent studies, exposure to fat-rich diets may be the actual cause of obesity. Dietary long-chain fatty acids affect brain function and are linked to food intake and motivation-related behaviors. Recently, many studies have shown that different types of fatty acids play different roles in animals. In our study, the effects of stearic acid (a saturated fatty acid) and oleic acid (a monounsaturated fatty acid) in diets on hedonic feeding behaviors were investigated, and changes of feeding-related protein levels in the brain were detected to explore the possible mechanism underlying the effects of these fatty acids. As a result, mice fed a diet containing stearic acid, compared to a diet containing oleic acid, exhibited increased food intake, hedonic eating, and an operant response to sucrose and locomotor activity. Furthermore, stearic acid corresponded to a higher level of leptin in serum than oleic acid. In addition, the stearic acid treated group had lower protein levels of p-JAK2 and p-STAT3 in the VTA and a higher dopamine concentration in the NAc than the oleic acid-treated group. Meanwhile, the protein level of TH in the NAc was higher and the protein level of the DA transporter in the VTA was lower in the stearic acid-fed group than in the oleic acid-fed group. In conclusion, these findings indicated that a diet containing stearic acid can increase hedonic feeding behavior and affect mesolimbic dopamine system signals in mice. Moreover, the lowering of serum leptin and leptin signaling in the VTA may contribute to this effect.

Short-term (3-day) consumption of a high fat diet (HFD) rich in saturated fats is associated with a neuroinflammatory response and subsequent cognitive impairment in aged, but not young adult, male rats. This exaggerated effect in aged rats could be due to a “primed” microglial phenotype observed in the normal aging process in rodents in which aged microglia display a potentiated response to immune challenge. Here, we investigated the impact of HFD on microglial priming and lipid composition in the hippocampus and amygdala of young and aged rats. Furthermore, we investigated the microglial response to palmitate, the main saturated fatty acid (SFA) found in HFD that is proinflammatory. Our results indicate that HFD increased gene expression of microglial markers of activation indicative of microglial priming, including CD11b, MHCII, CX3CR1, and NLRP3, as well as the pro-inflammatory marker IL-1β in both hippocampus and amygdala-derived microglia. Furthermore, HFD increased the concentration of SFAs and decreased the concentration of polyunsaturated fatty acids (PUFAs) in the hippocampus. We also observed a specific decrease in the anti-inflammatory PUFA docosahexaenoic acid (DHA) in the hippocampus and amygdala of aged rats. In a separate cohort of young and aged animals, isolated microglia from the hippocampus and amygdala exposed to palmitate in vitro induced an inflammatory gene expression profile mimicking the effects of HFD in vivo. These data suggest that palmitate may be a critical nutritional signal from the HFD that is directly involved in hippocampal and amygdalar inflammation. Interestingly, microglial activation markers were increased in response to HFD or palmitate in an age-independent manner, suggesting that HFD sensitivity of microglia, under these experimental conditions, is not the sole mediator of the exaggerated inflammatory response observed in whole tissue extracts from aged HFD-fed rats.

Metabolic inflammation (metaflammation) is characteristic of obesity-related metabolic disorders, associated with increased risk of development of type 2 diabetes, nonalcoholic fatty liver disease (NAFLD), or cardiovascular disease. Metaflammation refers to a chronic, low-grade systemic inflammation as opposed to the classical transient and acute inflammatory responses of the innate immune system. Metaflammation is driven by a range of adverse dietary factors, including saturated fatty acids and some sugars, suggesting that certain dietary triggers may be particularly relevant beyond simple excessive dietary intake presenting as obesity. Importantly, obese patients with diabetes have a higher risk of infection and display gut microbiota profiles characteristic of dysfunctional immunity. Targeting metaflammation has also emerged as a strategy to attenuate metabolic disease. In this review we explore how different nutrition interventions may reconfigure disrupted metabolic inflammation in type 2 diabetes and nonalcoholic fatty liver disease by reestablishing a conventional proinflammatory program in innate immune cells and/or correcting dysbiosis to dampen systemic inflammation. We begin by reviewing concepts of metabolic inflammation relating to IL-1β inflammation and how it is induced by dietary and/or metabolic stressors. We then explore whether and how dietary interventions may attenuate processes pertaining to metaflammation, either directly or indirectly via the microbiome. Hence, we hope to bring new perspectives to alleviate the metaflammation typifying metabolic disease.

The recommendation to limit dietary saturated fatty acid (SFA) intake has persisted despite mounting evidence to the contrary. Most recent meta-analyses of randomized trials and observatio...

Adipose tissue and the liver play significant roles in the regulation of whole-body energy homeostasis, but they have not evolved to cope with the continuous, chronic, nutrient surplus seen in obesity. In this review, we detail how prolonged metabolic stress leads to adipose tissue dysfunction, inflammation, and adipokine release that results in increased lipid flux to the liver. Overall, the upshot of hepatic fat accumulation alongside an insulin-resistant state is that hepatic lipid enzymatic pathways are modulated and overwhelmed, resulting in the selective buildup of toxic lipid species, which worsens the pro-inflammatory and pro-fibrotic shift observed in nonalcoholic steatohepatitis.

Changes in the physiological state and adhesion of probiotics under stresses affect their interaction with the host. The effects of osmotic (3% NaCl, 6% NaCl, and 3% NaCl + 3% KCl), acid (pH 5.0), and alkali (pH 8.0) stress on the physiological characteristics, adhesion ability and pathogen adhesion inhibition of Lactobacillus plantarum KLDS 1.0328 were investigated. The results showed that 6% NaCl resulted in lower acid production, growth and antimicrobial activity of cells compared to control and 3% NaCl treatment. Reduced surface hydrophobicity, aggregation ability, adhesion ability and pathogen adhesion inhibition were observed when L. plantarum KLDS 1.0328 was exposed to 6% NaCl. These changes were weakened when NaCl was partially replaced by KCl. Exposure to stresses other than alkali stress significantly increased the ratio of unsaturated fatty acid to saturated fatty acid in the cell membrane. The autoaggregation and adhesion ability of cells were increased under pH 5.0 treatment. The results demonstrated that the abilities of cells to adhere and inhibit pathogen adhesion were significantly positively correlated with the ability to coaggregate with pathogens. This study provides a basis for our understanding of the response of L. plantarum to stresses and the related molecular mechanisms.

Cross talk between endothelial cells and adipocytes is vital to adipocyte functions, but little is known about the mechanisms or factors controlling the process. Angiogenesis is a critical component linking the endothelium to healthy adipogenesis, yet it is not known if or how it is involved in adipocyte physiology. Therefore, the purpose of this study was to determine the effect of angiopoietin-1 (Ang-1) and -2 (Ang-2) as well as their receptor, Tie-2, on adipocyte physiology. 3T3-L1 pre- and mature adipocytes were found to express Ang-1, Ang-2, and Tie-2, which decrease upon polyunsaturated fatty acid treatment. Furthermore, 3T3-L1 cells treated with recombinant Ang-1 or Ang-2 increased expression of the antiapoptotic gene Bcl-x and decreased expression of the proapoptotic gene Casp-8. Next, preadipocytes were treated with saturated fatty acids (SFAs) to induce cell stress. SFA-mediated splicing of X-box-binding protein-1 was reduced by co-treatment with Ang-1, and cell viability was improved in the presence of SFAs + Ang-1. Taken together, these results indicate that Ang-1 may protect preadipocytes from SFA-induced apoptosis and endoplasmic reticulum stress.

© 2020 Tam et al. Obesity and elevation of circulating free fatty acids are associated with an accumulation and proinflammatory polarization of macrophages within metabolically active tissues, such as adipose tissue, muscle, liver, and pancreas. Beyond macrophages, neutrophils also accumulate in adipose and muscle tissues during high-fat diets and contribute to a state of local inflammation and insulin resistance. However, the mechanisms by which neutrophils are recruited to these tissues are largely unknown. Here we used a cell culture system as proof of concept to show that, upon exposure to a saturated fatty acid, palmitate, macrophages release nucleotides that attract neutrophils. Moreover, we found that palmitate up-regulates pannexin-1 channels in macrophages that mediate the attraction of neutrophils, shown previously to allow transfer of nucleotides across membranes. These findings suggest that proinflammatory macrophages release nucleotides through pannexin-1, a process that may facilitate neutrophil recruitment into metabolic tissues during obesity.

Several conditions are marked by increased susceptibility to, and enhanced severity of, bacterial infections. Alcohol use disorder, one of these conditions, is known to predispose to bacterial pneumonia by suppressing the lung's innate immune system, and more specifically by disrupting critical alveolar macrophage (AM) functions. Recently, we established that chronic ethanol consumption also perturbs surfactant lipid homeostasis in the lung and that elevated concentrations of free fatty acids contribute to blocking essential AM functions, such as agonist-induced cytokine expression. In this study, we extend these observations by showing that elevated free fatty acid levels impair metabolic responses to lipopolysaccharide (LPS) in AMs. In particular, we show that the glycolytic reprogramming characteristic of LPS-stimulated AMs is blunted by the saturated fatty acid palmitate, whereas oleate, an unsaturated fatty acid, or ethanol alone, had no effect on this adaptive metabolic response. Additionally, we found that elevated concentrations of palmitate induced mitochondrial oxidative stress and that glycolytic reprogramming and cytokine production to LPS could be partially restored in AMs by either pharmacologically blocking palmitate entry into mitochondria or administering a mitochondrial-specific antioxidant. Taken together, these findings suggest that alcohol and elevated levels of saturated fatty acids conspire to impair pulmonary innate immunity by altering metabolic responses in AMs. Additionally, our findings suggest that targeting the mechanisms involved in fatty acid metabolism can restore pulmonary immunity and possibly limit bacterial pneumonia in individuals with alcohol use disorder.

Background and aims Because of the conflicting research results, the association between saturated fatty acid (SFA) consumption and the risk of stroke remains controversial. We conducted a meta-analysis to evaluate potential dose–response relation between SFA intake and stroke. Methods and results PubMed, Embase, the Cochrane Library Central Register of Controlled Trials, and Web of Science were searched. Summary relative risks (RRs) of the highest vs. the lowest category of SFA intake and their 95% confidence intervals (CIs) were pooled by random-effects models. Linear or nonlinear dose–response trend estimations were evaluated with data from categories of SFA consumption in each study. Fourteen studies involving a total of 598,435 participants were eligible for high vs. low meta-analysis, and 12 studies involving a total of 462,268 participants were eligible for the dose–response relation assessment. Higher dietary SFA intake was associated with a decreased overall risk for stroke (RR, 0.87; 95% CI, 0.78–0.96; I2 = 37.8%). A linear relation between SFA and stroke was explored (P = 0.01), the pooled RR of stroke per 10 g/day increase in SFA intake was 0.94 (95% CI, 0.89–0.98; P = 0.01). Conclusion This meta-analysis further demonstrated that a higher consumption of dietary SFA is associated with a lower risk of stroke, and every 10 g/day increase in SFA intake is associated with a 6% relative risk reduction in the rate of stroke. Further research is needed to explore the influence of specific SFA types and different macronutrient replacement models of SFA on the stroke risk.

Objective: To investigate the association of postprandial and fasting plasma saturated fatty acid (SFAs), monounsaturated fatty acid (MUFAs) and polyunsaturated fatty acid (PUFAs) concentrations with hand and knee osteoarthritis (OA).Design: In the population-based NEO study clinical hand and knee OA were defined by the ACR classification criteria. Structural knee OA was defined on MRI. Hand and knee pain was determined by Australian/Canadian Hand Osteoarthritis Index (AUSCAN) and KOOS, respectively. Plasma was sampled fasted and 150 min after a standardized meal, and subsequently analysed using a nuclear magnetic resonance platform. Logistic regression analyses were used to investigate the association of total fatty acid, SFA, MUFA, total PUFA, omega-3 PUFA and omega-6 PUFA concentrations with clinical hand and knee OA, structural knee OA and hand and knee pain. Fatty acid concentrations were standardized (mean 0, SD 1). Analyses were stratified by sex and corrected for age, education, ethnicity and total body fat percentage.Results: Of the 5,328 participants (mean age 56 years, 58% women) 7% was classified with hand OA, 10% with knee OA and 4% with concurrent hand and knee OA. In men, postprandial SFAs (OR (95% CI)) 1.23 (1.00; 1.50), total PUFAs 1.26 (1.00; 1.58) and omega-3 PUFAs 1.24 (1.01; 1.52) were associated with hand OA. SFAs and PUFAs were associated with structural, but not clinical knee OA. Association of fasting fatty acid concentrations were weaker than postprandial concentrations.Conclusion: Plasma postprandial SFA and PUFA levels were positively associated with clinical hand and structural knee OA in men, but not in women. (C) 2019 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

The aim of this research was to study the influence of the composition of saturated fatty acids of biodiesel on the performance, emissions and characteristics of combustion in an engine with compression ignition. An increase in saturated fatty acids may eliminate biodiesel deficiencies. Various 55% mixtures of saturated fatty acids with diesel fuel were studied and compared. Five different combinations of blend were prepared using the following esters such as Palm oil methyl ester (POME), Coconut oil methyl ester (COME), Neem oil methyl ester (NOME), Mahua oil methyl ester (MOME), Jatropha oil methyl ester (JOME) and Pongamia oil methyl ester (PONOME). All five combinations (BD1-BD5) were tested and compared for physicochemical characteristics such as density, viscosity, iodine number, saponification number, energy consumption, thermal efficiency, exhaust gas temperature, toxic gas emissions. Five blended combinations showed viscosity around 5.13 to 5.56 centistokes. The Cetane number of the five blends were in the range of 52–56. The heating value of the five combinations was in the range of 38.92 to 40.65 MJ/kg. Iodine values were found to be in the range of 50–73. The brake thermal efficiency percentage were in the range of 27.15–27.4. All five blends found to emits less amount of Greenhouse gases. The results of the research state that all five combinations of biodiesel are unique, and they show very good performance properties as fuel along with less discharges toxic emissions.

Background & Aims The fatty acid translocase, also known as CD36, is a well-established scavenger receptor for fatty acid (FA) uptake and is abundantly expressed in many metabolically active tissues. In the liver, CD36 is known to contribute to the progression of non-alcoholic fatty liver disease and to the more severe non-alcoholic steatohepatitis, by promoting triglyceride accumulation and subsequent lipid-induced endoplasmic reticulum (ER) stress. Given the recent discovery that the hepatocyte-secreted proprotein convertase subtilisin/kexin type 9 (PCSK9) blocks CD36 expression, we sought to investigate the role of PCSK9 in liver fat accumulation and injury in response to saturated FAs and in a mouse model of diet-induced hepatic steatosis. Methods In this study, we investigated the role of PCSK9 on the uptake and accumulation of FAs, as well as FA-induced toxicity, in a variety of cultured hepatocytes. Diet-induced hepatic steatosis and liver injury were also assessed in Pcsk9-/- mice. Results Our results indicate that PCSK9 deficiency in cultured hepatocytes increased the uptake and accumulation of saturated and unsaturated FAs. In the presence of saturated FAs, PCSK9 also protected cultured hepatocytes from ER stress and cytotoxicity. In line with these findings, a metabolic challenge using a high-fat diet caused severe hepatic steatosis, ER stress inflammation and fibrosis in the livers of Pcsk9-/- mice compared to controls. Given that inhibition of CD36 ablated the observed accumulation of lipid in vitro and in vivo, our findings also highlight CD36 as a strong contributor to steatosis and liver injury in the context of PCSK9 deficiency. Conclusions Collectively, our findings demonstrate that PCSK9 regulates hepatic triglyceride content in a manner dependent on CD36. In the presence of excess dietary fats, PCSK9 can also protect against hepatic steatosis and liver injury. Lay summary The proprotein convertase subtilisin/kexin type 9 (PCSK9) is a circulating protein known to reduce the abundance of receptors on the surface of liver cells charged with the task of lipid uptake from the circulation. Although PCSK9 deficiency is known to cause lipid accumulation in mice and in cultured cells, the toxicological implications of this observation have not yet been reported. In this study, we demonstrate that PCSK9 can protect against cytotoxicity in cultured liver cells treated with a saturated fatty acid and we also show that Pcsk9 knockout mice develop increased liver injury in response to a high-fat diet., Graphical abstract Unlabelled Image, Highlights • PCSK9 reduces the expression of hepatic CD36 in mice. • CD36 is a known driver of liver steatosis and injury. • PCSK9 protects from palmitate-induced ER stress and ROS in cultured hepatocytes. • High-fat diet causes severe hepatic steatosis, ER stress, inflammation and insulin resistance in Pcsk9-/- mice. • PCSK9 expression protects mice from diet-induced liver injury.

The toxicity of polychlorinated biphenyls (PCBs) and their transformation have been intensively investigated in recent years. However, the potential mechanisms of biotransformation in a lake food web remain unclear. To explore the correlation between the PCBs and δ 15 N values or FAs, six fish species were collected from Dongting Lake, and various tissues were dissected to analyze the δ 15 N values, FAs and PCBs. Based on the wet weight (ww), the concentration of ∑PCBs 115 ranged from 0.04 to 9.77 ng g −1 , and the highest level was found in the gonad of Cyprinus carpio . The toxicity equivalent (TEQ) of PCBs ranged from 0.003 to 2.39 pg g −1 , and the highest level was found in the fat of Silurus asotusy . The PCB levels in fish collected from Dongting Lake were at the low end of the global range. PCB28, 52, 95, 99, 101, 105 110, 118, 138, 153, 155 and 209 were found in all tissues. PCBs were distributed in a tissue-specific and species-specific manner in fish. PCB153 and 138 had a positive correlation with the TEQ in liver, gill, intestine and skin of fish on the basis of lipid weight (lw). Docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) and polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) had a positive correlation with some PCB congeners in the intestine. PCB52, ∑tetra-PCBs and Ind-PCBs had a positive correlation with the δ 15 N values in liver of fish on the basis of ww. PUFA/SFA and DHA/EPA might be indicators for the transfer of PCB congeners.

This study investigated the long-term effect of dietary fatty acid saturation on eggshell quality and bone characteristics of laying hens at end of lay. Five isoenergetic (3,011.5 kcal AME/kg DM) and isonitrogenous (169 g CP/kg DM) diets were formulated using different lipid sources at a constant 30 g/kg inclusion level. The control, polyunsaturated omega-3 (PUFA n-3), polyunsaturated omega-6 (PUFA n-6), monounsaturated omega-9 (MUFA n-9), and saturated fatty acid (SFA) diets consisted of linseed- and fish oil (50:50 blend), fish oil, sunflower oil, high oleic acid sunflower oil, and tallow, respectively. A total of 200 individually caged Hy-Line Silver-Brown hens (20 wk of age) were randomly allocated to the 5 treatments (n = 40 replicates/treatment) and received the experimental diets for 54 wk. During weeks 58, 62, 66, 70, and 74 of age (end-of-lay period), 20 eggs/treatment per day (n = 140 eggs/treatment per week) were selected for determining eggshell quality traits. At 74 wk of age, 10 birds per treatment (n = 10 birds/treatment) were randomly selected for the determination of bone quality characteristics. Data were statistically analyzed (P0.05) using a fully randomized 1-way ANOVA. Dietary treatment had no effect (P0.05) on eggshell quality traits. The MUFA n-9 treatment with the highest unsaturated to SFA ratio (UFA: SFA) resulted in a higher (P0.05) femur weight (10.34 g) as well as femur- (52.99%) and tibia ash (51.07%) content than the SFA treatment. Also, the PUFA n-3 diet resulted in a higher (P0.05) femur weight (10.21 g), femur ash (51.82%), and percentage femur (0.57%) compared to the SFA diet. Results suggested that prolonged feeding of diets varying in FA profile had no negative effect on eggshell quality, whereas UFA: SFA and long-chain n-3 PUFA affected the bone quality (especially the femur) of hens at the end of lay.

Eco-friendly alternative way for waste straw management is needed. Black soldier fly (Hermetia illucens) larvae (BSFL) are outstanding insects for bioconversion of organic waste, which can convert separate or mixed waste, i.e. manure, kitchen waste and straw, into biomass rich in protein and fat. In this study, through orthogonal experiment, maize straw, fermented with Aspergillus oryzae for 24 h in a ratio of 4000:1, was identified for BSFL bioconversion. Bioconversion performance of BSFL on fermented maize straw was assessed. With this fermented maize straw as feedstuff, larval stage of BSF was significantly prolonged compared with the control feedstuff (wheat bran). Adult duration was significantly shortened and the fecundity of females was significantly decreased. The harvested dry BSF larvae contained 41.76% crude protein, 30.55%, crude fibre, and 8.24% crude ash. The grease of BSFL, compared to the larvae collected from the control diet, had a higher proportion of monounsaturated fatty acid (24.86%) and polyunsaturated fatty acid (25.37%), while a lower proportion of saturated fatty acid (45.41%). Dry matter reduction of maize straw was 48.41%. After bioconversion by BSFL, the residual maize straw was significantly changed in crude protein, crude fat and nitrogen free extract content. By comparing with organic fertilizer NY525-2012, maize straw residue had a potential application in agriculture and nursery as a novel fertilizer. This new alternative method will benefit the comprehensive bioconversion of maize straw with pretreatment by insect.

Biodiesel has gained much attention as it is a promising renewable substitute fuel to conventional diesel in the future. Although biodiesel has numerous advantages such as lower toxicity, higher flash point and its biodegradability over conventional diesel, the challenges of its poor cold flow properties and oxidation stability limit its application. This is attributed to the high level of polyunsaturated fatty acid methyl esters in the biodiesel feedstock. Oxidation stability is determined by the fatty acid compositions and natural antioxidants of the biodiesel. A higher amount of unsaturated fatty acid methyl esters are susceptible to oxidative degradation and this in turns shortens the induction period and improves the cold flow properties of the biodiesel. Conversely, a full saturated fatty acid also results in higher cetane number and increases the oxidation stability of the biodiesel but tends to worsen the cold flow properties and this may result in low heating value and high viscosity which may affect the engine in a negative way during lower temperature climate especially winter. Partial hydrogenation of biodiesel is one of the techniques to improve the challenges of cold flow properties and oxidation stability. This review paper seeks to summarize the developments in terms of the production, cold flow properties, oxidation stability, catalytic material for the hydrogenation process, and feedstock for the production and ways to reduce trans-isomers in hydrogenated biodiesel.

Oil palm inflorescence ash (FMA) has been used in post-harvest processing and preservation of food crops. In this study effect of oil palm inflorescence ash as a processing aid on the chemical composition and metagenomics of fermented African oil bean seed (Pentaclethra macrophylla, Benth) was investigated. The result showed that the African oil bean seeds sample fermented with FMA had higher total amino acid (TAA) content (88.48 g/100 g crude protein (cp)) and better protein quality compared to the sample fermented without the ash (NFM) (81.04 g/100 g cp) and the unfermented (UFM) sample (8.07 g/100 g cp). The FMA had lower residual anti-nutritional factors and total saturated fatty acid (4.07%) hence has better dietary value than the NFM and UFM samples. New aromatic volatile compounds were also produced in the FMA which further improved its sensory quality compared to the NFM and UFM samples. The metagenomic study revealed that FMA had fewer spoilage bacteria but higher fermentative yeasts than the NFM. Thus the findings of the current study provide evidence that African oil bean seeds fermented with oil palm inflorescence ash have better chemical and microbial composition than the one processed without the oil palm ash.

Background The fatty acid (FA) composition of membrane phospholipid reflects at least in part dietary fat composition. Saturated FA (SFA) suppress Sirt1 activity, while monounsaturated FA (MUFA) counteract this effect. Objective We explored a role of Sirt1 in homeostatic control of the fatty acid composition of membrane phospholipid in the presence of SFA overload. Methods and results Sirt1 deficiency in cardiomyocytes decreased the expression levels of liver X receptor (LXR)-target genes, particularly stearoyl-CoA desaturase-1 (Scd1), a rate-limiting enzyme in the cellular synthesis of MUFA from SFA, increased membrane SFA/MUFA ratio, and worsened left ventricular (LV) diastolic function in mice fed an SFA-rich high fat diet. In cultured cardiomyocytes, Sirt1 knockdown (KD) exacerbated the palmitate overload-induced increase in membrane SFA/MUFA ratio, which was associated with decrease in the expression of LXR-target genes, including Scd1. Forced overexpression of Scd1 in palmitate-overloaded Sirt1KD cardiomyocytes lowered the SFA/MUFA ratio. Nicotinamide mononucleotide (NMN) increased Sirt1 activity and Scd1 expression, thereby lowering membrane SFA/MUFA ratio in palmitate-overloaded cardiomyocytes. These effects of NMN were not observed for Scd1KD cardiomyocytes. LXRα/βKD exacerbated palmitate overload-induced increase in membrane SFA/MUFA ratio, while LXR agonist T0901317 alleviated it. NMN failed to rescue Scd1 protein expression and membrane SFA/MUFA ratio in palmitate-overloaded LXRα/βKD cardiomyocytes. The administration of NMN or T0901317 showed a dramatic reversal in membrane SFA/MUFA ratio and LV diastolic function in SFA-rich HFD-fed mice. Conclusion Cardiac Sirt1 counteracted SFA overload-induced decrease in membrane phospholipid unsaturation and diastolic dysfunction via regulating LXR-mediated transcription of the Scd1 gene.

Projet AbsoFrom.; International audience; The study was designed to compare the effect of diets of equal calcium and fat content from pressed-cooked cheese, butter, palm oil or a chow diet, on blood lipids, faecal fat excretion and body composition in rats. Palm oil intake resulted in higher faecal saturated fatty acid (SFAs) excretion compared with pressed-cooked cheese or butter, showing the importance of the positional distribution of SFAs in triacylglycerol. However, compared with butter, cheese fat intake resulted in higher faecal fat excretion, confirming the importance of the dairy-matrix effect in the excretion of fat. Despite a high consumption of fat, animals of pressed-cooked cheese group did not gain weight (no differences in any groups) and did not accumulate fat compared with the control group, contrary to the situation with rats fed with the same amount of fat provided by vegetable oil and extracted from butter that gained fat mass compared with controls.

The effects of lipid composition, crystallization temperature, Solid fat content (SFC), microstructure on the rheological properties of blends of fully hydrogenated palm kernel oil (FHPKO) or fully hydrogenated coconut oil (FHCNO) with rapeseed oil (RSO) in the range of 20–100% were investigated using small and large deformation rheology. The variation of chemical composition, crystallization temperature (5–30 °C), SFC of the blends resulted in different rheological properties. Shear modulus was positive correlation with the content of saturated fatty acid of the sample. The storage modulus (G′) and yield force increased with the addition of FHPKO or FHCNO and were dramatically influenced by SFC and crystallization temperature. The values of G′ in FHPKO/RSO blends were higher than those of corresponding FHCNO/RSO blends. Combined analysis of SFC and microstructure related to rheological behavior revealed that smaller average crystal diameter, higher SFC and higher fractal dimension led to higher storage modulus of fat crystal networks.

BackgroundDietary recommendations to limit red meat are based on observational studies linking intake to cardiovascular disease (CVD) risk together with the potential of its saturated fatty acid (SFA) content to raise low-density lipoprotein (LDL) cholesterol. However, the relation of white meat to CVD risk, and the effects of dietary protein source on lipoprotein particle subfractions, have not been extensively evaluated.ObjectiveWe tested whether levels of atherogenic lipids and lipoproteins differed significantly following consumption of diets with high red meat content compared with diets with similar amounts of protein derived from white meat or nonmeat sources, and whether these effects were modified by concomitant intake of high compared with low SFAs.MethodsGenerally healthy men and women, 21-65 y, body mass index 20-35 kg/m2, were randomly assigned to 1 of 2 parallel arms (high or low SFA) and within each, allocated to red meat, white meat, and nonmeat protein diets consumed for 4 wk each in random order. The primary outcomes were LDL cholesterol, apolipoprotein B (apoB), small+medium LDL particles, and total/high-density lipoprotein cholesterol.ResultsAnalysis included participants who completed all 3 dietary protein assignments (61 for high SFA; 52 for low SFA). LDL cholesterol and apoB were higher with red and white meat than with nonmeat, independent of SFA content (P&nbsp

An experiment was conducted to study the effect of solid state fermentation of de-oiled rice bran (DORB) with Rhizopus oryzae on in vitro protein digestibility, anti-nutritional factors and fatty acid profile. The fermentation of DORB with Rhizopus oryzae significantly reduced the in vitro protein digestibility of DORB (p

Fourier transform infrared microspectroscopy (FTIR) is a promising method for the analysis of biological samples. Recent studies reported that FTIR imaging allows determination of the distribution of several biomolecules in a sample with no staining or extraction. In this study, FTIR was used to monitor biochemical changes in C. elegans nematodes cultured in nematode maintenance media (CeMM) without supplementation and with supplementation with either a long chain polyunsatured omega 3 fatty acid, eicosapentaenoic acid (EPA) or a saturated fatty acid, palmitic acid (PA) at 100 μM. EPA is an omega 3 fatty acid with documented health benefits while PA is generally consumed in diets. Worms were placed on BaF2 slides, and FTIR spectra were collected from single worms in transmission mode using a focal plane array detector. Principal component analysis grouped the FTIR spectra into three clusters corresponding to spectra of worms cultured with no supplementation, worms cultured with supplementation with EPA, and worms cultured with supplementation with PA. The major differences between the FTIR spectra reside in the vibrations corresponding to unsaturated fatty acids (3008 cm−1), lipids (2928, 2848, and 1744 cm−1), and proteins (1680, 1648, and 1515 cm−1). This indicates that supplementation with EPA or PA leads to biochemical alterations related to unsaturated fatty acids, lipids, and proteins. Furthermore, supplementing mutant strains (tub-1 and fat-3) CeMM with PA resulted in the appearance of the vibration 3008 cm−1, an increase in the intensity of the vibration 1744 cm−1, and a new vibration at 1632 cm−1, which is assigned to the amide I of β-pleated sheet component of proteins, in the spectra of tub-1 and fat-3 mutant strains. The results illustrated the potential use of FTIR alongside other techniques such as gas chromatography and staining techniques to investigate lipid metabolism and fat accumulation as well as induced changes in protein structures.

GPR173 is a highly conserved G protein coupled receptor associated with the hypothalamic-pituitary-gonadal reproductive axis. It is expressed in the brain and ovaries, however considerable knowledge about its function remains unknown. One putative ligand for this receptor is phoenixin (PNX), a newly identified reproductive peptide involved in hypothalamic coordination of the estrous cycle. In order to characterize GPR173, it is vital to determine how Gpr173 is regulated in the hypothalamus. Since the hypothalamus senses compounds from the blood, such as nutrients and chemicals, we examined the effect of palmitate, a saturated fatty acid, and bisphenol A (BPA), an endocrine disrupting chemical, on Gpr173 gene expression. Immortalized hypothalamic neurons were treated with palmitate or BPA for 2–24 h and Gpr173 mRNA levels were assessed with RT-qPCR. Palmitate and BPA both reduced Gpr173 mRNA levels, in part through the mitogen-activated protein kinase (MAPK), p38. Pre-treatment with palmitate for 24 h blocked the PNX-induction of phosphorylated cAMP response element-binding protein (CREB) levels. In conclusion, nutrition levels and environmental chemicals may influence reproductive function through modulation of Gpr173 expression, which may prove to be a future therapeutic target in reproductive health.

This study aimed to evaluate the effect of the process time (5, 10, and 15 min) and flow rate (10, 20, and 30 mL/min) of cold plasma technology on physio-chemical characteristics (pH), bioactive compounds (DPPD, Total Phenolic Compounds, ACE-inhibitory activity values), fatty acid composition, and volatile compounds profile of chocolate milk drink. The mild (lower flow rate and process time) and more severe (higher flow rate and process time) conditions led to a reduction of the bioactive compounds (total phenolic compounds and ACE-inhibitory activity), changes in fatty acid composition (increased saturated fatty acid and decreased monounsaturated fatty acid and polyunsaturated fatty acid), less favorable health indices (higher atherogenic, thrombogenic and hypercholesterolemic saturated fatty acids and lower desired fatty acids), and lower number of volatile compounds. In contrast, in intermediate cold plasma conditions, an adequate concentration of bioactive compounds, fatty acid composition, and health indices, and increased number of volatile compounds (ketones, esters, and lactones) were observed. Overall, cold plasma technology has proven to be an interesting alternative to chocolate milk drinks, being of paramount importance the study of the cold plasma process parameters.

Vibrio parahaemolyticus is one of the major pathogens caused diseases in cultured mud crab (Scylla paramamosain). Mud crabs lack an adaptive immune system, their defenses depend almost on innate immunity. Evaluation of the molecular responses of mud crabs to pathogens is essential for control of disease occurrence in farmed animals. In this study, the impacts of V. parahaemolyticus on immunity-related genes and metabolites in mud crabs of different groups (PG, SG and MG refer to controlled, survival and moribund groups, respectively) were investigated. Our results revealed that V. parahaemolyticus infection stimulated significant expressions of immune-related genes (prophenoloxidase, alpha 2-macroglobulin, lysosomal-associated membrane protein, Rab5, C-type lectin B and anti-lipopolysaccharide factor 5) in the MG within 72 h post-infection. The ATP content was significantly reduced in all tissues except muscle of moribund mud crabs. A total of 668 metabolites (including 190 down-regulated and 145 up-regulated) were identified and assigned to 77 pathways in both SG and MG. Metabolites involved in the saturated fatty acid are up-regulated, whereas unsaturated fatty acid and amino acid metabolisms are down-regulated in the immune system of mud crabs during the bacterial infection in MG. Furthermore, a reduction of hemocyte number and an increase of microbial abundance was found in MG. Our results demonstrated that V. parahaemolyticus induced death of mud crabs through reducing the metabolites associate with energy biosynthesis and innate immune system (i.e. proliferation of hemocyte and melanization), resulting in decrease of ATP in different tissues and failed to clearance of pathogens, respectively. The findings of this study provide a basic information of the responses of mud crab on bacterial infection, which is essential for prevention and control of diseases in mud crab aquaculture.

Petroleum is one of the most important pollutants in the marine ecosystem, and oil spills have a long-term effect on the marine environment. After an oil spill, petroleum continues to diffuse, flowing to the intertidal zone where it accumulates. Ulva pertusa is the main macroalgae species in the intertidal zone of the ocean, and petroleum is toxic to marine macroalgae. Fatty acids, which are involved in various physiological activities, including metabolism, are indispensable substances in organisms. Thus, an organism's fatty acid composition reflects its physiological state. Because fatty acids are carbon-rich compounds, their stable carbon isotope composition can be measured to assess the state of the organism. In this study, the effects of the water accommodated fraction (WAF) of different concentrations of 180# fuel oil and 0# diesel oil on the chlorophyll a and fatty acid carbon stable isotope composition (δ13CFAs) of U. pertusa were assessed. The fatty acid carbon stable isotope value varied among the different petroleum treatments, and high WAF concentrations significantly inhibited the synthesis of unsaturated fatty acids. Thus, exposure to petroleum products affected the synthesis of fatty acids in U. pertusa and also caused carbon stable isotope fractionation during the fatty acid synthesis process. The results indicate that the saturated fatty acid δ13C16:0 and the unsaturated fatty acid δ13C18:1 can be used as biomarkers to evaluate the degree of petroleum-induced stress in U. pertusa and that carbon stable isotope analysis of U. pertusa can be used as an environmental risk assessment tool in the intertidal zone.

Saturated fatty acid (SFA) intake is associated with obesity, insulin resistance, and hepatic steatosis, but scant work examines the impact of SFA type upon these outcomes. We tested the hypothesis that an obesogenic diet prepared with medium chain SFA (MCSFA), mostly as lauric acid-derived from coconut oil, reduces obesity-induced outcomes compared to obesogenic diets prepared with increasing amounts long chain SFA (LCSFA), primarily palmitic acid. Mice were fed (16 weeks) a control, low fat diet or obesogenic diets prepared with differing content of MCSFA or LCSFA in which polyunsaturated and monounsaturated fatty acids (PUFA; MUFA) were kept constant. Inclusion of MCSFA in an obesogenic diet prevented hepatic lipid accumulation and lowered indices of insulin resistance. Obesogenic diets reduced hepatic levels of de novo lipogenesis proteins (SCD1 and FASN) but elevated the adipose levels of mRNA for the pro-inflammatory markers Mcp-1 and Tnfα. Lipidomic analysis of plasma indicated that MCSFA intake resulted in a different lipidomic signature than LCSFA intake, prevented elevation of pro-inflammatory ceramides, but elevated concentrations of some lipids associated with elevated cardiovascular disease risk. Intake of the obesogenic diets in an SFA-type dependent manner elevated plasma concentrations of several phosphatidylcholine (PC) lipids having the long chain PUFA (LCPUFA) arachidonic acid (ARA) and docosahexaenoic acid (DHA), altered phospholipid ethers, and changed the triacylglyceryl environments of these LCPUFA. Our data indicate that (1) MCSFA reduce the severity of some obesogenic co-morbidities, (2) SFA-type modulates lipidomic signatures associated with cardiovascular disease and diabetes, and (3) dietary SFA type impacts LCPUFA metabolism.

An experimental study on the isobaric molar heat capacities of ethyl nonanoate and ethyl dodecanoate was performed at temperatures between 303 K and 393 K and at pressures between 0.1 MPa and 25.2 MPa. An increase of isobaric molar heat capacity with temperature increase was observed, and temperature was found to have a greater effect on isobaric molar heat capacity than pressure. Then the isobaric molar heat capacity data of 18 saturated fatty acid methyl and ethyl esters in literature were selected, to get a general correlation for the isobaric molar heat capacity of saturated fatty acid alkyl esters. The average absolute relative deviation and the maximum deviation of the present correlation from experimental data are lower than 0.70% and 3.82%, respectively. At last, to test the predictive ability of the proposed correlation, the heat capacities of ethyl nonanoate and ethyl dodecanoate were calculated. The average absolute relative deviation and the maximum deviation from our experimental results are 0.88% and 2.10%, respectively.

Background Both obesity and high dietary fat intake activate the nucleotide oligomerization domain–like receptor protein 3 (NLRP3) inflammasome. Objective We aimed to examine NLRP3 inflammasome activity in the airways of obese asthmatic patients after macronutrient overload and in immune cells challenged by inflammasome triggers. Methods Study 1 was a cross-sectional observational study of nonobese (n = 51) and obese (n = 76) asthmatic adults. Study 2 was a randomized, crossover, acute feeding study in 23 asthmatic adults (n = 12 nonobese and n = 11 obese subjects). Subjects consumed 3 isocaloric meals on 3 separate occasions (ie, saturated fatty acid, n-6 polyunsaturated fatty acid, and carbohydrate) and were assessed at 0 and 4 hours. For Studies 1 and 2, airway inflammation was measured based on sputum differential cell counts, IL-1β protein levels (ELISA), and sputum cell gene expression (Nanostring nCounter). In Study 3 peripheral blood neutrophils and monocytes were isolated by using Ficoll density gradient and magnetic bead separation and incubated with or without palmitic acid, LPS, or TNF-α for 24 hours, and IL-1β release was measured (ELISA). Results In Study 1 NLRP3 and nucleotide oligomerization domain 1 (NOD1) gene expression was upregulated, and sputum IL-1β protein levels were greater in obese versus nonobese asthmatic patients. In Study 2 the saturated fatty acid meal led to increases in sputum neutrophil percentages and sputum cell gene expression of Toll-like receptor 4 (TLR4) and NLRP3 at 4 hours in nonobese asthmatic patients. In Study 3 neutrophils and monocytes released IL-1β when challenged with a combination of palmitic acid and LPS or TNF-α. Conclusion The NLRP3 inflammasome is a potential therapeutic target in asthmatic patients. Behavioral interventions that reduce fatty acid exposure, such as weight loss and dietary saturated fat restriction, warrant further exploration.

The aim of this study was to evaluate the effects of dietary non-gossypol cottonseed oil (CSO) or cottonseed meal (CSM) and their interactions on the texture properties, structure, nutritional composition, and edible safety of egg yolk. A total of 162 24-wk-old Hy-line Brown laying hens were randomly allocated into 9 diet treatments with 6 replicates of 3 hens per cage. A 3 × 3 factorial design using corn soybean meal-based diets supplemented with 0, 6, or 12% CSM and 0, 2, or 4% CSO in place of soybean meal and soybean oil, respectively, was utilized. The experiment lasted for 8 wk. Eggs obtained from the CSO groups had an egg yolk gel structure, and the hardness of egg yolk increased significantly (P0.001) after 4°C storaged for 2 wk; the texture properties of eggs storage at 25°C had opposite trend. There were no differences in texture properties of fresh egg yolk among the different groups (P0.05). The saturated fatty acid (SFA) content of egg yolk increased in a CSO dose-dependent manner, whereas opposite effects (P0.001) were found in the monounsaturated fatty acids (MUFA) and the omega-3/omega-6 fatty acid ratio. CSO-containing diets significantly reduced the cholesterol content (P0.05). No significant difference was observed among the different groups on the contents of moisture, crude protein, crude fat, phospholipid, potassium, or iron (P0.05) in the egg yolk. Total gossypol residues were increased with the increased amount of CSM (P0.05), and these changes were independent of time (P0.05). The total gossypol concentration in the yolk was 2.01 to 5.16 mg/kg. These results suggest that CSO has a key influence on egg yolk quality, reducing both its taste and nutritional value. Egg yolk gelation was significantly associated with the change of fatty acid composition caused by CSO and storage conditions. Free gossypol will remain in the egg yolk. Although the residue is low, the edible safety risk of eggs maybe exists.

The study aimed to determine the fatty acid composition, vitamin E content of meat from light lambs in relation to slaughtering season (winter and spring) and age (45 and 60 days). In each season, twenty lambs were subdivided into two groups corresponding to the experimental ages. All lambs received maternal milk and a supplementation of hay and commercial concentrate from 30 days to slaughter. The total saturated fatty acid proportion was affected by the slaughter season, showing greater values in the spring compared to the winter, with significant difference for 60-day lambs. In winter, the meat showed a greater monounsaturated fatty acid proportion for both the 45-day and 60-day lambs, and the lowest n-6/n-3 fatty acid ratio in lambs of 45 days. The higher contents of polyunsaturated fatty acid were obtained in the spring. In this season, the meat from 60-day lambs contained greater proportions of conjugated linoleic acids than that from the lambs slaughtered in the winter. The age and slaughter season were not associated with the differences in nutritional indices. The greater slaughter age improved the vitamin E content of meat. In conclusion, the meat from the older lambs had better properties as vitamin E and polyunsaturated fatty acid contents in spring; in winter, monounsaturated fatty acids resulted higher in both 45 and 60-d lambs.

Background Elevated low-density lipoprotein (LDL) cholesterol is a major risk factor for cardiovascular disease. Dietary guidance recommends reducing saturated fatty acid, trans fatty acid, and cholesterol intakes to reduce circulating LDL cholesterol. Cholesterol intake may also affect high-density lipoprotein (HDL)-cholesterol concentrations, but its impact has not been fully quantified. Objectives The aims of this study were to investigate the dose-response relation between changes in dietary cholesterol intake and changes in lipoprotein-cholesterol markers for cardiovascular disease risk and to provide a reference for clinicians on how changes in dietary cholesterol intake affect circulating cholesterol concentrations, after accounting for intakes of fatty acids. Methods We used a Bayesian approach to meta-regression analysis, which uses Markov chain Monte Carlo techniques, to assess the relation between the change in dietary cholesterol (adjusted for dietary fatty acids) and changes in LDL and HDL cholesterol based on the use of data from randomized dietary intervention trials. Results Fifty-five studies (2652 subjects) were included in the analysis. The nonlinear Michaelis-Menten (MM) and Hill models best described the data across the full spectrum of dietary cholesterol changes studied (0-1500 mg/d). Mean predicted changes in LDL cholesterol for an increase of 100 mg dietary cholesterol/d were 1.90, 4.46, and 4.58 mg/dL for the linear, nonlinear MM, and Hill models, respectively. Conclusions The change in dietary cholesterol was positively associated with the change in LDL-cholesterol concentration. The linear and MM models indicate that the change in dietary cholesterol is modestly inversely related to the change in circulating HDL-cholesterol concentrations in men but is positively related in women. The clinical implications of HDL-cholesterol changes associated with dietary cholesterol remain uncertain.

Background Dietary intakes of total and specific types of saturated fatty acids (SFAs) in relation to total mortality remain largely unknown in China. Objective We assessed the associations of total and individual SFA intakes with total mortality in a Chinese nationwide population. Methods This prospective analysis included 7888 women and 6495 men, aged >20 y, from the China Health and Nutrition Survey (1989-2011). Cumulative mean of SFA intake was calculated based on 3 d of 24-h records in each round of the survey. Multivariable Cox proportional hazard models were used to estimate HR and 95% CI. Results There were 1011 deaths during a median of 14 y of follow-up. In isocaloric nutrient density models that replace total carbohydrates, intakes of total SFAs and even-chain SFAs were associated with higher total mortality in women but not in men. Compared with the lowest quartiles, the HRs (95% CIs) in the highest quartiles were 1.65 (1.03, 2.62) for total SFAs (P-trend = 0.042) and 1.83 (1.13, 2.94) for even-chain SFAs (P-trend = 0.016) in women. Intake of odd-chain SFAs was associated with lower total mortality both in men [HR (95% CI): 0.64 (0.47, 0.87); P-trend = 0.01] and in women [HR (95% CI): 0.59 (0.42, 0.84); P-trend = 0.0013]. Moreover, intake of medium-chain SFAs was linked with lower total mortality in men [HR (95% CI): 0.64 (0.44, 0.93); P-trend = 0.013]. However, isocalorically replacing 1% of energy from unsaturated fatty acids with even-chain SFAs was associated with higher total mortality in men [HRs (95% CIs): 1.08 (0.97, 1.20) and 1.39 (1.20, 1.60) for replacing PUFAs and MUFAs, respectively]. Conclusions Total SFA and even-chain SFA intake was associated with higher total mortality in women, whereas intake of odd-chain SFAs was related to lower total mortality in both sexes. This analysis of cohort was registered at clinicaltrials.gov as NCT03281512.

The Healthy Diet Indicator (HDI), which is based on adherence to World Health Organization's (WHO) nutrition guidelines, is used worldwide. In 2015, the WHO updated the Fact Sheet for their recommended healthy diet. We investigated diet quality assessed by the updated HDI (HDI-2015) and factors related to adherence to this diet in a Japanese population. We conducted a cross-sectional study of 1370 participants from 8 workplaces, 1 college, and 2 communities. All participants completed a brief-type self-administered diet history questionnaire. The HDI-2015 assesses 7 components: fruits and vegetables, total fat, saturated fatty acid, polyunsaturated fatty acid, free sugar, dietary fiber, and potassium. Only 6.6% of subjects demonstrated high adherence to HDI-2015 (met ≥6 components), whereas 52.0% demonstrated moderate adherence (4–5 components), and 41.4% demonstrated low adherence (0–3 components). Male sex, older age, and regular physical activity were associated with higher adherence. The contemporary Japanese population has an overall low diet quality as evaluated by the updated HDI score. Improving adherence to healthier dietary patterns may promote better health in Japan., Highlights • Contemporary Japanese population has an overall low diet quality. • High adherers to WHO Healthy Diet Indicator are men, older age, and active persons. • High adherers associated with diets higher in beneficial nutrients and vitamins

In the present work we are reporting the synthesis and binding interaction of a saturated fatty acid containing 9-aminoacridine derivative (AC-PA) with ct-DNA and β-cyclodextrin (β-CD). From Steady-state fluorescence experiments this newly synthesized 9-aminoacridine derivative, AC-PA, shows more efficient binding interaction with ct-DNA as compared to the 9-aminoacridine (AC). The extent of interaction of AC-PA and AC with ct-DNA was found out by calculating the fluorescence quenching by using Stern–Volmer quenching equation. The calculated quenching constants of AC-PA and AC are (4.5 ± 0.5) × 103M−1 (3.7 ± 0.5) × 103M−1 respectively. The mechanism of fluorescence quenching of AC-PA and AC, were understand by using Stern–Volmer plots as well as time-resolved fluorescence experiments. The fluorescence quenching of AC-PA and AC by ct-DNA are static in nature and take place by formation of ground state complexes. The binding mode between AC-PA and AC were understood by DNA melting analysis experiment. The DNA melting analysis experiments were reveals that the binding interactions between fluorophores (AC-PA and AC) with ct-DNA are intercalative in nature. The melting temperature and mode of binding intercalative mode of binding between AC-PA and AC were further confirmed by DSC and CD experiments. The steady-state and time-resolved fluorescence parameters of AC-PA are quite sensitive towards the formation of inclusion complexes between AC-PA and β-CD. Long hydrophobic tail containing acridine conjugate (AC-PA) shows more efficient binding interactions with the β-CD and the calculated binding constants value of AC-PA is 0.51 × 102M−1. Whereas, the parent molecule, AC not showing any binding interactions with β-CD.